1,720,990 research outputs found

    Pseudomonas spp. isolated in diary herds. A new protocol with PCR real time by using “DUAL FRET” probes for ovine mastitis

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    A novel and rapid realtime polymerase chain reaction (PCR) molecular approach for the detection of Pseudomonas aeruginosa, P. fluorescens and P. putida in biofilms in sheep dairy farms is described. This strategy is based on early detection of the subsequent rate: Pseudomonas vs. total bacteria in biofilm specimens using particular bi-functional FRET oligonucleotide probes named DUAL-FRET

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Antibacterial activity of two products containing hydrogen peroxide and silver salts

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    Abstract Objectives The purpose of this study was to examine the antibiofilm and the antibacterial activity efficacy of H2O2 alone and combined with silver salts. Materials and methods We used standard and innovative methods to assess the antibacterial effects of (i) H2O2 at 300 e 600ppm (parts per million), and at 3%; (ii) the same H2O2 concentrations with the addition of Ag+ salts at 5 and 10ppm, against a large number of microorganisms, in planctonic and sessile (biofilm) forms. Results All of the microorganisms tested (including some nosocomial pathogens, such as Legionella pneumophila, Staphylococcus aureus, Pseudomonas aeruginosa) were inhibited at concentrations lower than those commonly used in dental unit waterlines (MIC < 600ppm) Conclusions H2O2 with silver salts displayed greater antibiofilm efficacy than the negative control (H2O2 without silver ions)

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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