1,218 research outputs found
Fabrication and Characterization of Fluidic Channel and Dispensing Nozzle Integrated Microcantilever Heaters
No full textIn this paper, microchannel and dispensing nozzle integrated microcantilever heaters are proposed as a new platform enabling drop-on-demand bubble jet printing with fast and efficient heating and real-time printing process monitoring for the first time. Microcantilever heaters with integrated microchannel and dispensing nozzle are batch fabricated via ion implantation and polysilicon sacrificial process. Then, local temperature of fabricated microcantilever heaters are calibrated with micro Raman thermometry. Temperature calibrated microcantilever heaters are characterized under various pulsed heating powers sufficient for bubble generation and bubble jet microdroplet dispensing with a reference printing solution. Upon pulsed heating, heating thermal time constants are extracted from both electrothennal and electrothermomechanical responses, each of which are analyzed with temperature-dependent electrical resistance and resonance frequency, respectively. Besides the fast and efficient heating capability, our proposed system can down modulate viscosity of non-Newtonian ink solutions via high-frequency shearing at various eigenmodes and monitor the size and position of thermally induced micro/nano bubbles within the microchannel via multiple eigenmodes measurements. These promising aspects will accelerate on-demand thermal bubble jet printing towards high-resolution printed electronics
An approach toward SNP detection by modulating the fluorescence of DNA-templated silver nanoclusters
No full textA rapid,homogeneous,andin-situlabelableformatdesignedtodetectsinglenucleotidepolymorphism
(SNP) ispresented.Fluorescentsilvernanoclustersproducedfollowinghydride-mediatedreductionof
Agþ boundtoapartialdouble-strandedoligodeoxynucleotidewereemployedasprobesforSNPs.The
sensingmechanismisbasedonthefluorescenceenhancementofsilvernanoclustersthroughan
irreversibleclustertransferuponhybridization.Thekeyelementofourdesignismodulatingthe‘‘turn
on’’ mechanismbyintroducingacompetitorthatcanbedisplacedinresponsetoatargetDNA.Ina
controlledmodelsystem,thefluorescenceintensityofsilvernanoclustersisapproximately3-fold
enhanceduponhybridizationwithaperfectlymatchedtargetDNA,andsingle-basemismatch
detectionisachievedwithin5min,regardlessofthepositionofmismatches.Humanaldehyde
dehydrogenase2(ALDH2),whichisresponsiblefortheoxidationofaldehydestocarboxylicacids,is
then utilizedasatargetforSNP.Uponadditionofaperfectlymatchedsequence,dramaticallyenhanced
fluorescenceintensityisobserved(ca.48-fold),andpromptSNPgenotypingisaccomplished.11sciescopu
sj-docx-1-tam-10.1177_17588359231189421 – Supplemental material for Pretreatment endocrine symptoms and recurrence-free survival among young premenopausal patients with breast cancer: a prospective cohort study
No full textSupplemental material, sj-docx-1-tam-10.1177_17588359231189421 for Pretreatment endocrine symptoms and recurrence-free survival among young premenopausal patients with breast cancer: a prospective cohort study by Danbee Kang, Juhee Cho, Seri Park, Hyo Jung Kim, Seok Won Kim, Jeong Eon Lee, Jonghan Yu, Se Kyung Lee, Ji-Yeon Kim, Seok Jin Nam and Yeon Hee Park in Therapeutic Advances in Medical Oncology</p
Detection of EGFR Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung Carcinoma
No full textThe detection of epidermal growth factor receptor (EGFR) mutation, based on tissue biopsy samples, provides a valuable guideline for the prognosis and precision medicine in patients with lung cancer. In this study, we aimed to examine minimally invasive bronchial washing (BW)-derived extracellular vesicles (EVs) for EGFR mutation analysis in patients with lung cancer. A lab-on-a-disc equipped with a filter with 20-nm pore diameter, Exo-Disc, was used to enrich EVs in BW samples. The overall detection sensitivity of EGFR mutations in 55 BW-derived samples was 89.7% and 31.0% for EV-derived DNA (EV-DNA) and EV-excluded cell free-DNA (EV-X-cfDNA), respectively, with 100% specificity. The detection rate of T790M in 13 matched samples was 61.5%, 10.0%, and 30.8% from BW-derived EV-DNA, plasma-derived cfDNA, and tissue samples, respectively. The acquisition of T790M resistance mutation was detected earlier in BW-derived EVs than plasma or tissue samples. The longitudinal analysis of BW-derived EVs showed excellent correlation with the disease progression measured by CT images. The EGFR mutations can be readily detected in BW-derived EVs, which demonstrates their clinical potential as a liquid-biopsy sample that may aid precise management, including assessment of the treatment response and drug resistance in patients with lung cancer
Development of mature forebrain organoids by spatially and temporally controlling neurodevelopmental patterning cues
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Generation of prostate cancer assembloids to investigate the role of cancer-associated fibroblasts in resistance to androgen-targeted therapy
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Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
No full textThe development of advanced tumor models has long been encouraged because current cancer models have shown limitations such as lack of three-dimensional (3D) tumor architecture and low relevance to human cancer. Researchers have recently developed a 3D in vitro cancer model referred to as tumor organoids that can mimic the characteristics of a native tumor in a culture dish. Here, experimental procedures are described in detail for the establishment of bladder tumor organoids from a carcinogen-induced murine bladder tumor, including culture, passage, and maintenance of the resulting 3D tumor organoids in vitro. In addition, protocols to manipulate the established bladder tumor organoid lines for genetic engineering using lentivirus-mediated transduction are described, including optimized conditions for the efficient introduction of new genetic elements into tumor organoids. Finally, the procedure for orthotopic transplantation of bladder tumor organoids into the wall of the murine bladder for further analysis is laid out. The methods described in this article can facilitate the establishment of an in vitro model for bladder cancer for the development of better therapeutic options.11Ysciescopu
Development of mature forebrain organoids by spatially and temporally controlling neurodevelopmental cues for schizophrenia modeling
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sj-tif-2-taj-10.1177_20406223231176175 – Supplemental material for Air pollution and mortality in patients with chronic obstructive pulmonary disease: a cohort study in South Korea
No full textSupplemental material, sj-tif-2-taj-10.1177_20406223231176175 for Air pollution and mortality in patients with chronic obstructive pulmonary disease: a cohort study in South Korea by Suna Kang, Yun Soo Hong, Jihwan Park, Danbee Kang, Hyunsoo Kim, Jin Lee, Woojin Kim, Sung-Won Kang, Eliseo Guallar, Juhee Cho and Hye Yun Park in Therapeutic Advances in Chronic Disease</p
A comparative study on the process efficiencies and microbial community structures of six full-scale wet and semi-dry anaerobic digesters treating food wastes
No full textThe purpose of this study was to investigate the effect of different types of food wastes on the process efficiencyand microbial community structures in full-scale anaerobic digesters and to identify parameters that affect thesecriteria. Six full-scale anaerobic digesters were investigated; three were operated under “wet” condition (totalsolids TS ≤10%), and three were run under “semi-dry” condition (10% ≤TS ≤20%). Removal efficiency ofvolatile solids was much higher in the wet digesters (75.2 ± 3.8%) than in the semi-dry digesters(42.6 ± 5.5%). The bacterial and archaeal communities were distinctly characterized by familiesPorphyromonadaceae, Sphingobacteriaceae, Syntrophomonadaceae, and Methanobacteriaceae in the wet digesters;and of Clostridiaceae, Patulibacteraceae, Pseudonocardiaceae, Lachnospiraceae, Rikenellaceae, andMethanomicrobiaceae in the semi-dry digesters. The discriminant parameters identified were TS content ofinfluent, concentration of total ammonia nitrogen and the ratio of soluble chemical oxygen demand (COD) toCOD in the digester.The purpose of this study was to investigate the effect of different types of food wastes on the process efficiencyand microbial community structures in full-scale anaerobic digesters and to identify parameters that affect thesecriteria. Six full-scale anaerobic digesters were investigated; three were operated under “wet” condition (totalsolids TS ≤10%), and three were run under “semi-dry” condition (10% ≤TS ≤20%). Removal efficiency ofvolatile solids was much higher in the wet digesters (75.2 ± 3.8%) than in the semi-dry digesters(42.6 ± 5.5%). The bacterial and archaeal communities were distinctly characterized by familiesPorphyromonadaceae, Sphingobacteriaceae, Syntrophomonadaceae, and Methanobacteriaceae in the wet digesters;and of Clostridiaceae, Patulibacteraceae, Pseudonocardiaceae, Lachnospiraceae, Rikenellaceae, andMethanomicrobiaceae in the semi-dry digesters. The discriminant parameters identified were TS content ofinfluent, concentration of total ammonia nitrogen and the ratio of soluble chemical oxygen demand (COD) toCOD in the digester.1
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