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Erratum: Engraftment, neuroglial transdifferentiation and behavioral recovery after complete spinal cord transection in rats (Surg Neurol Int 2018 9 :19 DOI: 10.4103/sni.sni_369_17)
No full textIn the article titled “Engraftment, neuroglial transdifferentiation and behavioral recovery after complete spinal cord transection in rats”, published on pages 19, Issue 1, Volume 9 of Surgical Neurology International,[1] the name of the authors is written incorrectly as The “How to cite this article” section should read correctly as “Luzzi S, Crovace AM, Lacitignola L, Valentini V, Francioso E, Rossi G, Invernici G, Galzio RJ, Crovace A”
Effects of GLUBRAN-2 on the Burst Pressure of Jejunal Loops Thermofused With Vessel Sealing Devices
No full textBackground: Off-label use of radiofrequency vessel sealing devices for intestinal thermofusion has been reported as an alternate approach for closing the small and large intestines. The study aimed to evaluate if reinforcing the thermofusion line with a modified N-butyl-2-CyanoAcrylate and methacryloxysulpholane produced improved burst pressure values in ex vivo swine jejunal loops. Materials and Method: A suture-less full-thickness jejunal biopsy was performed with different radiofrequency vessel-sealing devices (Ligasure 5 mm: RFVS-1; Atlast 10 mm: RFVS-2; Cayman Maryland: RFVS-3), and reinforcement with modified cyanoacrylate Glubran-2 (G2) at the thermofusion defect was applied. Burst pressure(BP) values were compared with a control group, wherein a cold blade was utilized to obtain the biopsy, followed by the closing of the jejunum with seven Gambee sutures. Results: Seventy (n = 70) jejunal loop samples were distributed into the experimental groups.The RFVS-1 and -2 groups exhibited BP values similar to those of the suture group. The RFVS-3 group showed significantly lower BP values (P < 0.05) than the suture group. Conversely, in the groups wherein G2 was applied, all BP values were comparable to those of the suture group. BP test in the RFVS-3G2 group showed significantly (P < 0.05) higher values in the group using the same instrument without the glue (RFVS-3). Conclusions: G2 has been shown to improve the BP on the defects created by instruments that are not completely efficient in intestinal thermofusion and sealing. This experimental model showed that the performance of full-thickness biopsies with RFVS devices and reinforcement with G2 provide feasible and promising results
Biomechanical evaluation of an absorbable fixation strap for use in total laparoscopic gastropexy in dogs
No full textOBJECTIVE To compare load-to-failure results for laparoscopic absorbable fixation straps (AFSs) deployed at various angles and for AFSs versus absorbable knotless (barbed) suture when used in simulated total laparoscopic gastro-pexy (TLG) in specimens from cadaveric dogs. SAMPLE 30 stomach and abdominal body wall specimens. PROCEDURES Specimens were assigned to 1 of 3 groups for use in simulated TLG con-structs for comparisons of load-to-failure results for single AFSs deployed at 30°, 60°, or 90° (AFS-angle group; n = 10) or for a gastropexy span of 4 to 5 cm achieved with 3-0 absorbable knotless (barbed) monofilament suture applied in a simple continuous pattern (TLG-1; 10) versus 8 AFSs applied with a deployment angle > 30° (TLG-2; 10). A 1-way ANOVA was used to compare results among AFS deployment angles (30°, 60°, or 90°) and between TLG-1 and TLG-2. RESULTS Mean ± SD load to failure for the AFS-angle group was significantly higher for the AFS deployment angles of 60° (8.00 ± 3.90 N) and 90° (12.71 ± 8.00 N), compared with 30° (5.17 ± 1.90 N). However, no substantial difference was detected in the mean ± SD load to failure for TLG-1 (39.18 ± 7.1 N) versus TLG-2 (31.43 ± 10.86 N). CONCLUSIONS AND CLINICAL RELEVANCE Results of the present study supported the potential use of AFSs in gastro-pexy in dogs; however, prospective clinical research with adequate long-term follow-up is warranted before recommendations can be made
Survival of mesenchymal stem cells in collagenase induced tendonitis in an ovine model.
Full text linkThe pourpose of this study was to evaluate the efficacy and
the survival of local injection of allogenic MSC marked with
Red Fluorescent Protein (RFP) (Lentigen-Italy) in
collagenase induced tendonitis in the ovine Achille's
tendon.The study was performed after the approval by the
National Animal Care and Use Committee
Four sheep (2 years old, female, 45 bwt) have been
enrolled in the study. After some days for the acclimatation,
the sheep have been investigated to exclude any previous
Achilles' tendon lesion. Three weeks before starting of the
study one sheep was randomly selected for Bone Marrow
harvesting for MSCs cultivation. The MSCs obtained has
been trasfected with a lentivirus for integration of a gene for
expression of Red Fluorescent Protein (RFP). After a week
the other 3 sheep was injected in both Achilles' tendon with
400 U.I. of Collagenase IA of Cl. hystoliticum (Sigma-
Aldrich-Italy). After two weeks the left Achilles' tendon of
each sheep was injected with a solution of 6x106 RFPMSCs
( MSCRFP ) in 1 ml of fibrine glue (TISSUCOL ,
Baxter). The remaining tendons were used as negative
control and received the same volume of saline solution as
placebo. At 3-4-6 weeks from the treatment the tendons
were harvested and evaluated for morphology, collagen I
and III expression, and visualized at fluorescence
microscope to assess RFP expression of the grafted
MSCRFP. The results of these investigations evidenced
the presence of MSCRFP in the treated
tendons respect to the control ones at 3, 4 and 6 weeks after
the treatment. Moreover, the RFP positive tissue showed high
expression of collagen I and low collagen III with good
morfphology in comparison to the lesions treated with
placebo.The presence of high expression of collagen I and
low collagen III with good morfopholgy, in term of restored
tendon architecture, can be related to the MSCRFP injected
into tendon lesions, as a large number of cells can survive in
the site of injection.
These results showed that intralocal administration of MSCs
into the tendon lesion can lead to a good effect on injured
tendon . The local infusion delivery entails injecting MSCs
directly into the tissue of interest and guarantees a higher
number of engrafted cells and optimal therapuetic effect.
Besides the survival of high numbers of positive RFP -MSCs
in treated samples have been demonstrated at 3, 4 (Fig.1)
and 6 weeks from the treatment. We have evaluated also that
quality of tendon healing in MSCRFP treated tendons has
been based on a better architecture of collagen fibers and
high expression of collagen I respect to collagen III (Fig.2),
related to the control tendons.
The data obtained in this study confirm that MSCs allograft
have a positive effect on tendon healing, its lack of significant
immunogenicity permitting allogenic transplantation without
immunosuppressive drugs and that the local injection in the
tendon allows the survival of MSCs with good engraftment
effincency
Activation of PI3-kinase/Akt induced small bowel cell apoptosis during laparoscopic ischaemia-reperfusion of swine jejunum
No full textEx-Vivo Evaluation of “First Tip Closing” Radiofrequency Vessel Sealing Devices for Swine Small Intestinal Transection
No full textThis study compared burst pressure (BP), number of activations, and histological assessment of ex vivo swine small intestine loops transected by stapler, a single fulcrum radiofrequency vessel sealing (RFVS) device, and the newly-developed jaws RFVS. Fifty (n = 50) 20 cm long jejunal loops were randomly assigned to be transected with RFVS devices and linear stapler (Caiman5, Caiman Maryland, Caiman12, Ligasure Atlas, and Stapler group as control respectively). Caiman5, Caiman12 and stapler required only one activation to complete the sealing. The mean BP in Caiman5 and Caiman Maryland groups were significantly lower (p < 0.05) than the S group as control and the other RFVS devices studied. RFVS Caiman12 and Ligasure Atlas produced mean BP values that were close to the Control and did not differ between them. The lumen was totally closed in the Caiman12 and Ligasure Atlas groups. The findings of this investigation were promising; we discovered that Caiman12 and Ligasure Atlas produce comparable mechanical capabilities as well as stapled intestinal closure, however Caiman12 need a single activation to complete the transection
Surgery plus chondroprotection for canine cranial cruciate ligament (CCL) rupture: a proton-NMR study.
No full textErratum of Surgery plus chondroprotection for canine cranial cruciate ligament (CCL) rupture: a proton-NMR study.
Vet Comp Orthop Traumatol. 2006;19(4):239-4
Histology and immunohistochemistry study of ovine tendon grafted with cBMSCs and BMMNCs after collagenase-induced tendinitis.
No full textSurvival of bone marrow mesenchymal stem cells labelled with red fluorescent protein in an ovine model of collagenase-induced tendinitis
Full text linkObjective: The aim of this study was to track the survival and efficacy of allogeneic bone marrow mesenchymal stem cells (BM-MSC) marked with red fluorescent protein (BM-MSCRFP) in an ovine model of collagenase-induced tendinopathy. Methods: Bone marrow was harvested from one donor sheep and BM-MSC were isolated, cultivated and transfected with red fluorescent protein (BM-MSCRFP). Collagenase was injected into both Achilles tendons in the remaining nine sheep. After two weeks the left tendon was injected with a solution of 6 x 106 BM-MSCRFP and fibrin glue, while only fibrin glue was administered to the contra-lateral tendon in each sheep. After three, four and six weeks the tendons were harvested and evaluated for morphology, collagen I deposition, presence of CD34+ cells, and fluorescent labelled BM-MSC. Results: We demonstrated that delivery of BM-MSC into tendon lesions had positive effects on the injured tendons. The BM-MSCRFP survived at three, four and six weeks after treatment, leading to better quality healing of tendons as compared to the controls, where no labelled cells were detected. Interestingly, we demonstrated high expression of CD34+ cells in tendons that had been treated with BM-MSCRFP. Clinical relevance: Mesenchymal stem cell allografts have a positive effect on tendon healing and local injection of BM-MSC directly into the tendon allows the homing of BM-MSC for good efficiency of engraftment
1H NMR investigation of normal and osteo-arthritic synovial fluid in the horse.
No full textProton magnetic resonance spectroscopy (1H NMR) has been successfully used in the study of many biological fluids. The data presented here report on the metabolic profiles of normal equine synovial fluids compared with osteoarthritic (OA) fluids. Twenty-five OA synovial fluid samples and eight normal ones were collected from the forelimb fetlock joint in 22 horses, aged between five and 24 years. 1H NMR spectroscopy was carried out with a Bruker Avance DRX 500 equiped with a cryo-magnet working at 11 Tesla, and 'Mestre-C 4.9.9.6' software was used to analyze the spectra. The study assessed the increase of lactate, alanine, acetate, N-acetylglucosamine, pyruvate, citrate, creatine/creatinine, glycerol, HDL choline, and a-glucose in OA synovial fluid. The variations observed in samples from horses with OA compared to those in the control group, and similar data found in other studies, confirm that this technique may be useful in the study of joint metabolism. Its practical application may be in the evaluation of the treatment of OA in athletic horses
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