16 research outputs found

    FIGURE 3 in Massaria broussonetiae sp. nov. and M. racemosae sp. nov. (Massariaceae, Pleosporales) on moraceous hosts

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    FIGURE 3. Massaria racemosae (holotype, MFLU 19–2135) a Substrate. b, c Ascomata on substrate. d Vertical section of an ascoma. e Peridium. f Paraphyses. g–l Asci (l in Congo Red). m–t Ascospores (s in Congo Red, t in Indian ink). Scale bars: a = 1 cm, b = 1000 µm, c = 500 µm, d = 200 µm, g–l = 50 µm, e, m–s = 20 µm, f = 5 µm.Published as part of Samarakoon, Milan C., Liu, Jian-Kui, Karunarathna, Samantha C., Mukjang, Nilita & Cheewangkoon, Ratchadawan, 2022, Massaria broussonetiae sp. nov. and M. racemosae sp. nov. (Massariaceae, Pleosporales) on moraceous hosts, pp. 263-274 in Phytotaxa 559 (3) on page 269, DOI: 10.11646/phytotaxa.559.3.4, http://zenodo.org/record/702174

    The use of surface immunofluorescence assay (SIFA) in the microbiological diagnosis of Lyme borreliosis: a case report

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    In this report we describe the use of a newly developed immunofluorescence technique performed with living spirochetes to detect serum antibody to B. burgdorferi s.l. in a case of early Lyme borreliosis. The immunofluorescence method used (surface immunofluorescence assay: SIFA) proved useful in the serological evaluation of suspected cases of Lyme disease

    Functional Activities of Antibodies Directed against Surface Lipoproteins of Borrelia hermsii

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    Enriched preparations for mouse polyclonal immunoglobulin G (IgG) antibodies reactive with surface-exposed epitopes (Ab-SEE) of the 22-kDa and 24-kDa membrane lipoproteins of living Borrelia hermsii (HS 1 strain) cells were obtained by an antibody absorption technique using living spirochetes. In vitro, the antibody preparations both inhibited spirochetal growth and were borreliacidal in the presence of complement. The monovalent Fab antibody fragments, prepared from antibody-enriched preparations, did not inhibit the growth of the bacteria, whereas they killed the bacteria in the presence of complement. The two-dimension gel electrophoresis of B. hermsii cells showed that 3H-labeled fatty acids incorporated into the 22-kDa and 24-kDa lipoproteins were resolved into one and three compact spots, respectively. The spots were recognized by the Ab-SEE preparations reactive with the 22-kDa and 24-kDa proteins, by Western blotting. © 1995, Center For Academic Publications Japan. All rights reserved

    Thermoplastic disks used for commercial orthodontic aligners: complete physicochemical and mechanical characterization

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    Invisible orthodontic aligners (IOAs) have been introduced in the orthodontic field as an innovative alternative for fixed brackets, in relation to their ability to be easily inserted/removed from the oral cavity without affecting the chewing ability and the aesthetic of the patients. The paper provides a complete physicochemical and mechanical characterization of thermoplastic materials in the form of disks used for commercial IOAs. A wide palette of specific techniques is considered, from tensile tests and dynamic-mechanical analysis, to X-Ray diffraction (XRD), differential scanning calorimetry (DSC), Fourier transformation infrared spectroscopy (FTIR-ATR) analyses and water absorption tests. The disks are investigated before and after immersion into staining beverages (red wine, coffee, nicotine and artificial saliva), in terms of colour variations, transparency, and microscopic surface modifications by means of colorimetry, UV-VIS absorbance and scanning electron microscopy (SEM). Among all the samples, polyurethane (PU) exhibited the highest crystallinity and the highest values of mechanical and thermal resistance, while the poly(ethylene terephthalate)-glycol (PETG) samples presented better transparency and less ability to absorb water. Moreover, red wine and coffee give noticeable colour variations after 14 days of immersion, together with a slight reduction of transparency
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