29 research outputs found
Screening and identification of Piper species as rootstocks resistance against the root knot nematode under glasshouse condition
Get PDFA study was conducted to evaluate the reaction of different Piper species to root knot nematode (Meloidogyne incognita) and the nematode reproduction were studied in the nematode infested pot culture experiment. Three Piper species viz., Piper colubrinum, Piper argyrophyllum and cultivated Piper nigrum varieties of IISR Sakthi, IISR Thevam, Panniyur-1 and Karimunda were screened. In this study, seven rootstocks were evaluated for resistance based on the growth of pepper cuttings and resistance indexes to Meloidogyne incognita, which were surveyed 45 days after inoculation with M. incognita. The observation was recorded viz., nematode soil population (200 cc), number of galls, number of egg mass, number of females, gall index. All the Piper species show varying degree of response. Out of seven rootstocks used in this experiment IISR Sakthi were found to be highly resistant, Piper colubrinum, IISR Thevam and Karimunda resistant to root knot nematode and these cultivars can be used as a source of resistance. Black pepper (Piper nigrum L.), in particular, suffer extensive damage due to root-knot nematodes, and only a few wild species are known to be resistant. Grafting of cultivated plants to rootstocks of known resistant Piper species rootstocks could be an effective method to resolve this problem
A Study on Adoption Level of Black Gram VBN 8 among Farmers of Pudukkottai District, Tamil Nadu
Get PDFPulses are important in Indian agriculture because of their high protein content (17–25%) compared to grains (6–10%), as well as their capacity to fix atmospheric nitrogen and to enhance soil fertility. Black gram (Vigna mungo L.), also known as Urd bean, Urid, or mash, is a major pulse crop. The study on adoption of Black gram VBN 8 was conducted in Pudukkottai district of Tamil Nadu. Out of 13 blocks in the district, three blocks were selected based on the area under black gram VBN 8 cultivation. The data collected were analysed and presented using descriptive statistics. The results of the study revealed that three fifth (60.80%) of the respondents belonged to medium level of adoption category, followed by 20.00 per cent and 19.20 per cent of the respondents in low and high level of adoption categories
Multiple shoot regeneration in kokum and camboge
Get PDFKokum (Garcinia indica Choisy) and camboge (Garcinia gummi-gutta (L.) Rob.), widely distributed in the Western Ghats remained neglected even though they are highly valued in South Indian cuisine and in traditional systems of medicine. Recently Garcinia has excited the scientific community for the indication that the fruit rind is the richest natural source of hydroxy citric acid, a potential anti obesity agent (Heymsfield et al. 1998; Muthulakshmi & Sarah 1999). The present paper deals with development of tissue culture protocols for multiplication of kokum and camboge
Multiple shoot regeneration in kokum and camboge
Get PDFKokum (Garcinia indica Choisy) and camboge (Garcinia gummi-gutta (L.) Rob.), widely distributed in the Western Ghats remained neglected even though they are highly valued in South Indian cuisine and in traditional systems of medicine. Recently Garcinia has excited the scientific community for the indication that the fruit rind is the richest natural source of hydroxy citric acid, a potential anti obesity agent (Heymsfield et al. 1998; Muthulakshmi & Sarah 1999). The present paper deals with development of tissue culture protocols for multiplication of kokum and camboge
Multiple shoot regeneration in kokum and camboge
Get PDFKokum (Garcinia indica Choisy) and camboge (Garcinia gummi-gutta (L.) Rob.), widely distributed in the Western Ghats remained neglected even though they are highly valued in South Indian cuisine and in traditional systems of medicine. Recently Garcinia has excited the scientific community for the indication that the fruit rind is the richest natural source of hydroxy citric acid, a potential anti obesity agent (Heymsfield et al. 1998; Muthulakshmi & Sarah 1999). The present paper deals with development of tissue culture protocols for multiplication of kokum and camboge
Effect of Probiotic Yeast, Sacchromyces cerevisiae on Tissue Biochemical and Carotenoid Variation in Freshwater Ornamental Fish Carassius auratus (Linnaeus, 1758)
No full textThe present work aims at assessing Bakers’s yeast, Saccharomyces cerevisiae as an efficient and eco-friendly probiotic feed additives for ornamental fish, Carassias auratus. For this study an indoor culture experiment was carried out 300l plastic trough containing 200l water in triplicate. In each tank five fishes at fingerlings stage (1.58 ± 0.14g) were reared for a period of 30 days. An uniform water quality parameters such as temperature (28.0 ± 1.0 0C), pH (7.60 ± 0.12), dissolved oxygen (4.80 ± 0.82mg/l) and ammonia (< 0.08 mg/l) were maintained. The results inferred that, the percentage increase in the biochemical constituents in both skin and muscle tissue of C. auratus received 2% yeast added diet (EDY2) registered higher values when compared to control and other experimental diets fed fishes. The total carotenoid content in the acetone extracted skin tissues of C. auratus fed with 2% yeast supplemented diet varied from 0.748+ 0.042 in 0 day to 0.944 + 0.014 µg/g wet tissue on 30th day of experiment. Likewise, in the muscle tissue of C. auratus extracted with acetone, the total carotenoid varied from 0.376+0.014 (0 day) to 0.541 + 0.008 µg/g wet tissue (30 day). The carotenoid content of both the tested tissues on methanol extract was low, when compared with acetone extract. The overall results inferred that S. cerevisiae supplementation @ 2g/ 100g diet distingly altered the carcass composition of C. auratus and also the accumulation of cartenoids in skin and muscle tissues. S. cerevisiae contains bioactive lead molecules, which accelerated the vital physical and metabolic activities in host fish species
Dynamic localization of the Na<sup>+</sup>-HCO<sub>3</sub><sup>-</sup> co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia
No full textFinely tuned regulation of transport protein localization is vital for epithelial function. The Na+-HCO3− co-transporter NBCn1 (also known as SLC4A7) is a key contributor to epithelial pH homeostasis, yet the regulation of its subcellular localization is not understood. Here, we show that a predicted N-terminal β-sheet and short C-terminal α-helical motif are essential for NBCn1 plasma membrane localization in epithelial cells. This localization was abolished by cell–cell contact disruption, and co-immunoprecipitation (co-IP) and proximity ligation (PLA) revealed NBCn1 interaction with E-cadherin and DLG1, linking it to adherens junctions and the Scribble complex. NBCn1 also interacted with RhoA and localized to lamellipodia and filopodia in migrating cells. Finally, analysis of native and GFP-tagged NBCn1 localization, subcellular fractionation, co-IP with Arl13B and CEP164, and PLA of NBCn1 and tubulin in mitotic spindles led to the surprising conclusion that NBCn1 additionally localizes to centrosomes and primary cilia in non-dividing, polarized epithelial cells, and to the spindle, centrosomes and midbodies during mitosis. We propose that NBCn1 traffics between lateral junctions, the leading edge and cell division machinery in Rab11 endosomes, adding new insight to the role of NBCn1 in cell cycle progression.Finely tuned regulation of transport protein localization is vital for epithelial function. The Na+-HCO3- co-transporter NBCn1 (also known as SLC4A7) is a key contributor to epithelial pH homeostasis, yet the regulation of its subcellular localization is not understood. Here, we show that a predicted N-terminal β-sheet and short C-terminal α-helical motif are essential for NBCn1 plasma membrane localization in epithelial cells. This localization was abolished by cell-cell contact disruption, and co-immunoprecipitation (co-IP) and proximity ligation (PLA) revealed NBCn1 interaction with E-cadherin and DLG1, linking it to adherens junctions and the Scribble complex. NBCn1 also interacted with RhoA and localized to lamellipodia and filopodia in migrating cells. Finally, analysis of native and GFP-tagged NBCn1 localization, subcellular fractionation, co-IP with Arl13B and CEP164, and PLA of NBCn1 and tubulin in mitotic spindles led to the surprising conclusion that NBCn1 additionally localizes to centrosomes and primary cilia in non-dividing, polarized epithelial cells, and to the spindle, centrosomes and midbodies during mitosis. We propose that NBCn1 traffics between lateral junctions, the leading edge and cell division machinery in Rab11 endosomes, adding new insight to the role of NBCn1 in cell cycle progression.</p
FISSR-PCR: a simple and sensitive assay for highthroughput genotyping and genetic mapping
Get PDFThe recently developed Inter-Simple Sequence Repeat PCR (ISSR-PCR) or microsatellite primed PCR or Simple Sequence Repeat (SSR)-Anchored PCR technique detects polymorphic markers in a wide variety of genomes. Usually the ISSR primers are either 5' end-labeled with γ[32P]ATP or one of the α[32P] labeled dNTPs is added to the PCR reaction and the PCR products are resolved on PAGE and autoradiographed. Alternatively, cold PCR products are resolved on agarose gel electrophoresis. In the present study, we show that informativity, sensitivity and speed of the ISSR-PCR can be substantially enhanced by adding fluorescent nucleotide in the PCR reaction followed by resolution of PCR products on an ABI 377 automated sequencer. The informativeness, measured as a number of detectable amplified fragments, was two-fold higher and the quantity of required template DNA is two-fold lower than the regular ISSR-PCR. We have termed this method as FISSR-PCR and show its usefulness in generating large number of species and varietal specific markers in plants, insects, parasites of insects and human and various infectious organisms. Further, we show that the FISSR markers are inherited and segregated in Mendelian fashion as demonstrated on a panel of 99 F2 offspring derived from a cross of two divergent silkworm strains. The FISSR-PCR marker assay could be a method of choice for large scale screening of varieties/cultivars and highthroughput genotyping in mapping of genomes where microsatellite information is scanty or absent
Sustainable potato production in a changing climate: Challenges and innovations
No full textClimate change presents a major threat to global agriculture, primarily due to the increasing frequency and intensity of extreme weather events. As the third most important food crop after rice and wheat, potatoes play a crucial role in achieving food security and alleviating malnutrition due to their high productivity and nutritional value. However, potato cultivation remains highly susceptible to environmental stressors, with projections indicating a potential 32 % reduction in tuber yield by 2050. Climate-induced stress influences the virulence and population dynamics of pests and pathogens, heightening the risk of sudden outbreaks due to increased host susceptibility. The development of stress-tolerant potato varieties and the extensive application of fungicides accelerate pathogen resistance evolution, ultimately limiting their long-term efficacy. Additionally, excessive chemical inputs elevate production costs and pose significant environmental risks. To mitigate these challenges, weather-based approaches offer a promising solution by enabling timely management practices through increasing the accuracy of real-time forecasting and dissemination of weather information to farming communities. The integration of proper agronomic practices, suitable breeding techniques and forecasting weather information with crop protection measures can enhance resilience to climate-induced stress. Adopting the Internet of Things (IoT) technologies in precision agriculture can optimize resource use, improve decision-making and contribute to global food security
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No full textNot AvailableThe disease outbreak in the aquaculture industry, coupled with ban on antibiotic usage due to residues in animal tissues and spreading of antimicrobial resistance ignites to find an alternative approach for control of
bacterial infections in aquaculture, especially in the larval stages of fishes. Use of organic acids holds a promising substitute to antibiotics. To study the efficacy of organic acids, a bath treatment experiment was
carried out in 12 L plastic tubs to find the effective concentration at which no mortality of larvae treated with
different organic acids viz.,acetic acid, lactic acid, malic acid, formic acid and citric acid concentrations for 10, 15, 30 and 60 minutes respectively. The milk fish larvae were treated with six concentrations(In percentage)
viz., 1, 0.2, 0.1, 0.075, 0.05, 0.025, with all the organic acids for different time intervals and later complete water was exchanged. The study was conducted separately for all organic acids at six different concentrations. Survival rates of the fish larvae were noted at the end of each acid treatment for respective time period and after 24 hours. Total mortality was observed at 1, 0.2, and 0.1 per cent for all organic acids for all time intervals. Results revealed that the effective concentrations at which no mortality observed were 0.05, 0.075, 0.05,
0.025 and 0.075 per cent for acetic acid, lactic acid, malic acid, formic acid and citric acid, respectively. From this study, it can be deduced that, the above organic acid concentrations may be used for treating the fish larvae at which no mortality observed, as a safe concentration. Further, these concentrations may enhance immune response and reduce the pathogenic microbial load (Vibrio spp.) from the larvae which cause mortality in stressed conditions.Not Availabl
