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Molecular genetics confirms the existence of different Management Units belonging to a single phylogenetic lineage within Italian populations of Alosa fallax (Lacepede, 1803)
The twaite shad Alosa fallax (Lacepede, 1803), belonging to the family Clupeidae, is a polytypic species
characterized by both landlocked and anadromous populations. These two forms have been known by historical
times in Italian waters and often considered as distinct taxonomic units. However, taxonomy of Italian shads has
been questioned, since it is based on morphological characteristics, not always reliable.
To clarify the phylogenetic relationships among Italian twaite shad populations, both landlocked and anadromous
ones were investigated by molecular markers, namely mitochondrial Cytochrome b (Cytb) gene sequencing. In
particular, new samples from Lake Omodeo (Sardina Island), Taro and Po Rivers were compared with previously
analyzed populations from Northern and Central Italy. Genetic analyses showed that the landlocked twaite
shad population from Lake Omodeo was characterized by a single Cytb haplotype (A. fallax hap1), whilst the
new samples from Taro River and Po River by two haplotypes (A. fallax hap3 and A. fallax hap5). These three
haplotypes are the most represented both in landlocked and anadromous Italian populations. These additional data
confirmed the occurrence of different Management Units (MUs) belonging to a single phylogenetic lineage within
Italian waters, without evidence of a separation at specific level of the A. fallax group. The Cytb phylogenetic
analysis do not support the existence of a separated species for Italian landlocked populations, as previously
hypothesized
Confirmed association between monoamine oxidase A molecular polymorphisms and Sudden Infant Death Syndrome.
We published in the February 2009 issue of Neurogenetics the article “Association of dopamine transporter and monoamine oxidase molecular polymorphisms with Sudden Infant Death Syndrome and stillbirth: new insights into the serotonin hypothesis” . The aim of our work was to investigate the contribution of additional neurotransmitters and related genes, different from the serotonin transporter (SLC6A4, 5-HTT) in the development of SIDS and stillbirth. The main conclusion discussed in the paper was that the frequency of MAOA allele 4R (having increased transcriptional activity) was statistically higher in SIDS and stillbirth than in the Control Group. The conclusions in our article have recently been submitted to criticism by Klintschar and Heimbold with a Letter to the Editor of Neurogenetics. We would kindly reply to their two main observations
Genes regulating neurotransmitters metabolism are associated with the Sudden Infant Death Syndrome and the stillbirth
Genetic data on endangered twaite shad (Clupeidae) assessed in landlocked and anadromous populations: One or more species?
Seven Italian populations of twaite shad Alosa fallax from Northern and Central Italy were investigated to assess genetic diversity by Cytochrome b (Cytb) gene sequencing. The two ecotypes historically referred to landlocked and anadromous populations were investigated for the first time from a genetic point of view, to clarify their phylogenetic relationships. Moreover, results obtained from populations coming from separated Adriatic and Tyrrhenian basins were compared with data assessed in samples of allis shad Alosa alosa from the Atlantic basin. All the Italian samples were recognized at species level as A. fallax, differing for five mutations from A. alosa. The analyses confirmed the occurrence of a single phylogenetic lineage and of a single species within Italian waters, in both landlocked and migratory populations. The minimum spanning network identified six haplotypes for A. fallax and two haplotypes for A. alosa. The neighbour-joining tree and the maximum likelihood on the Cytb gene sequences confirmed two distinct lineages for A. alosa and A. fallax, without evidence of a separation at specific level within the A. fallax group. A weak separation due to incipient population differentiation was detected between anadromous and landlocked Italian populations, supporting the idea of a recent separation. The molecular data herein collected do not support the existence of the already controversial incipient species Alosa agone. Despite this, the two ecotypes could be considered as different management units from a conservation viewpoint. © 2014 Springer International Publishing Switzerland
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