1,721,046 research outputs found

    Ethylene-responsive genes are differentially regulated during abscission, organ senescence and wounding in peach (Prunus persica)

    No full text
    Ethylene-responsive genes from peach (Prunes persica, L. Batsch) were isolated by differential screening of a cDNA library constructed from abscission zones in which cell separation had been evoked by treatment with the ethylene analogue propylene. DNA and deduced protein sequences of four selected clones, termed Prunus persica Abscission zone (PpAz), revealed homology to thaumatin-like proteins (PpAz8 and PpAz44), to proteins belonging to the PR4 class of pathogenesis-related (PR) proteins (PpAz89), and to fungal and plant beta-D-xylosidases (PpAz152). Expression analyses conducted on embrioctomized and CEPA-treated fruitlets as well as on fruit explants have shown that PpAz8, PpAz44 and PpAz89 are preferentially transcribed in the cells of the fruit abscission zone rather than in the non-zone tissues. The PpAz152 transcript showed a different accumulation pattern being consistently and promptly induced by wounding and only slightly stimulated by propylene. By contrast, a complex pattern of transcript accumulation was found for the four genes in response to the wounding of leaves and during organ development and senescence. Based on this evidence, the existence of multiple regulatory pathways underlying the differential expression of the four PpAz genes in the different tissues and physiological processes is hypothesized

    Identification of differential photosynthetic-related changes in response to temperature and light intensity in a ‘winter’ and ‘spring’ barley genotype and in the derived segregating doubled-haploid population

    No full text
    The winter cold-resistant parent 'Nure' is an Italian two-rowed feeding barley cultivar, while the spring and cold-sensitive parent 'Tremois' is a French two-rowed malting variety. The response of the two genotypes have been characterised under several conditions of acclimation and stress to abiotic factors (chilling and freezing temperature, high light intensity, high temperature) using chlorophyll fluorescence and gas exchange analysis. The genotypes were contrasting not only for different levels of stress tolerance acquired after acclimation but also for a different capacity to induce earlier responses to stress conditions; this was especially evident in variation of chlorophyll fluorescence parameters at high light intensity associated to acclimation temperature and in different sugars accumulation in leaves and roots at low temperature.A segregating population of doubled haploid (DH) lines was derived by anther culture from the F1 generation of the 'winter' x 'spring' barley cross 'Nure' x 'Tremois', and a first analysis of a total of 136 Doubled-Haploid lines has been carried out

    Genetic dissection of heading date and yield under Mediterranean dry climate in barley (Hordeum vulgare L.)

    No full text
    Understanding the genetics of drought tolerance is a major goal of plant breeders, especially in the Mediterranean region due to its sensitivity to drought and climate changes. Here we investigated genomic regions associated with heading date and yield under rainfed (R) and irrigated (I) conditions, along with some drought tolerance indices derived from phenotypic data collected on a panel of 83 European barley cultivars evaluated in field trials in Foggia, southern Italy, for three growing seasons. A genome wide association analysis (GWAS) was carried out using gene-based SNPs incorporated in the Illumina iSELECT Chip with known map position. A total of 4169 polymorphic SNPs were considered after filtering out SNPs with >10 % missing data and <10 % minimum allele frequency. Heritability was high for heading date and moderate for yield. Principle components analysis partitioned the barley germplasm in three subgroups characterized by different row-type and growth habit. A significant association with heading date was detected on chromosome 2HS, where the different alleles of associated markers were able to significantly affect heading date in different cultivars. Moreover, GWAS uncovered a significant association with yield and relative yield under R conditions on chromosome 5HS. These results provide a clue for further analysis on genetic control of barley flowering time and production in response to water stress under Mediterranean dry climate

    Going Beyond Counting First Authors in Author Co-citation Analysis

    Full text link
    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

    Full text link
    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Ethylene responsive genes are differentially regulated during peach fruit abscission

    No full text
    Ethylene responsive genes from peach (Prunus persica (L.) Batsch) were isolated by differential screening of a cDNA library constructed from abscission zones in which cell separation had been evoked by treatment with the ethylene analogue propylene. DNA and deduced protein sequences of four selected clones, termed as Prunus persica Abscission zone (PpAz), revealed homology to thaumatin-like proteins (PpAz8 and PpAz44), to proteins belonging to the PR4 class of pathogenesis related (PR) proteins (PpAz89), and to fungal and plant beta-D-xylosidases (PpAz152). Expression analyses conducted on embryoctomized and CEPA treated fruitlets as well as on fruit explants have shown that PpAz8, PpAz44 and PpAz89 are preferentially transcribed in the cells of the fruit abscission zone rather than in the non-zone tissues. PpAz152 transcript showed a different accumulation pattern being consistently and promptly induced by wounding and only slightly stimulated by propylene

    Appropriate Similarity Measures for Author Cocitation Analysis

    Full text link
    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
    corecore