2,754 research outputs found

    The Evolution of Extracellular Hemoglobins of Annelids, Vestimentiferans, and Pogonophorans.

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    The evolution of extracellular hemoglobins of annelids, vestimentiferans, and pogonophorans was investigated by applying cladistic and distance-based approaches to reconstruct the phylogenetic relationships of this group of respiratory pigments. We performed this study using the aligned sequences of globin and linker chains that are the constituents of these complex molecules. Three novel globin and two novel linker chains of Sabella spallanzanii described in an accompanying paper (Pallavicini, A., Negrisolo, E., Barbato, R., Dewilde, S., Ghiretti-Magaldi, A., Moens, L., and Lanfranchi, G. (2001) J. Biol. Chem. 276, 26384--26390) were also included. Our results allowed us to test previous hypotheses on the evolutionary pathways of these proteins and to formulate a new most parsimonious model of molecular evolution. According to this novel model, the genes coding for the polypeptides forming these composite molecules were already present in the common ancestor of annelids, vestimentiferans, and pogonophorans

    The evolution of extracellular hemoglobins of annelids, vestimentiferans, and pogonophorans.

    No full text
    The evolution of extracellular hemoglobins of annelids, vestimentiferans, and pogonophorans was investigated by applying cladistic and distance-based approaches to reconstruct the phylogenetic relationships of this group of respiratory pigments. We performed this study using the aligned sequences of globin and linker chains that are the constituents of these complex molecules. Three novel globin and two novel linker chains of Sabella spallanzanii described in an accompanying paper (Pallavicini, A., Negrisolo, E., Barbato, R., Dewilde, S., Ghiretti-Magaldi, A., Moens, L., and Lanfranchi, G. (2001) J. Biol. Chem. 276, 26384--26390) were also included. Our results allowed us to test previous hypotheses on the evolutionary pathways of these proteins and to formulate a new most parsimonious model of molecular evolution. According to this novel model, the genes coding for the polypeptides forming these composite molecules were already present in the common ancestor of annelids, vestimentiferans, and pogonophorans

    Scultura - Testina di Dioniso Barbato

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    Testina marmorea di Dioniso barbato ritenuta un tempo opera rinascimentale di imitazione dell\u27antico, per la quale è stata proposta l\u27ipotesi di una possibile antichità o quanto meno di una rielaborazione rinascimentale di un marmo antico frammentario. L\u27iconografia trova confronti nelle erme dionisiache di epoca ellenistica e romana; in particolare per l\u27acconciatura è interessante mettere in relazione il nostro esemplare con un\u27erma dei Musei Civici di Padova, datata al II secolo d.C.. La resa delle ciocche della barba e degli zigomi rimanda ad un\u27erma arcaistica ornamentale dei Musei Vaticani, datata genericamente a epoca romana, e ad un\u27altra erma del I secolo a.C. conservata a Roma presso il Palazzo dei Conservatori. Tracce di una rilavorazione, databile al XVI secolo, si individuerebbero invece nei particolari dell\u27attaccatura della barba e delle estremità dei baffi, oltre che nei fori di trapano in corrispondenza delle ghiandole lacrimali

    Replication data for "The Hamiltonian p-median Problem: Polyhedral Results and Branch-and-Cut Algorithms"

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    Dataset containing the experimental results of the research work "The Hamiltonian p-Median Problem: Polyhedral Results and Branch-and-Cut Algorithms"

    Reproduction of sphaerococcus coronopifolius (Gigartinales, Rhodophyta) in natural populations of the Lazio coasts (Central Italy) and in culture

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    As Sphaerococcus coronopifolius Stackhouse (Gigartinales, Rhodophyta) is a seaweed that has considerable potential for commercial uses, and as its life history in the various seas where it lives has not yet been thoroughly explored, we carried out a pilot study on the reproduction in nature and in vitro culture of field specimens established on the coasts of the Lazio region (Tyrrhenian Sea, central Italy). Spermatangia were not observed on the gametophyte (S. coronopifolius) and the tetrasporophyte of the species (Haematocelis fissurata) was not found either in the study site or in culture. As the carpospores developed directly into the gametophyte, we may conclude that this species reproduces itself, in both the study site and in culture, through a direct-type life history. It may thus be possible to use the variety of the clonal individuals growing on the coasts of Lazio for a future cultivation of S. coronopifolius on a large scale. © 2016 Adac. Tous droits réservés

    The pregnancy diagnosis in buffalo species: laboratory methods

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    Pregnancy diagnosis plays an important role in the reproduction management of ruminants since embryonic mortality has a substantial impact on the fertility of a herd. Most of the embryonic losses occur during the first days after fertilization and during the process of implantation. So it is very important to discriminate, with an early pregnancy diagnosis, non-pregnant from pregnant animals. Hormone analysis to detect pregnancy may be utilize as a more simple technique as an alternative of rectal palpation or ultrasound. In the last years, a large polymorphic family of placentaexpressed proteins has been discovered in ruminant species and used for pregnancy diagnosis. Members of this family are named pregnancy-associated glycoproteins (PAG), being synthesized in the mono-and binucleate cells of the ruminant’s trophectoderm. Part of them is released in the maternal blood circulation where they can be assayed by different laboratory techniques. Due to large variety of expressed molecules and to large variations in the posttranslational processing of the PAG, different immuno-systems present different ability to quantify the PAG released in blood. The assay of PAG can also bring very interesting information for researchers working in programs focused on the study of embryonic and fetal mortalities, as well as on embryo biotechnology, animal nutrition or infections diseases resulting in pathologies affecting the pregnancy

    Pregnancy-associated glycoproteins (PAGs): clinical application for pregnancy follow-up in Buffalo Cow

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    Pregnancy diagnosis is an important part in reproduction management of ruminant and pregnancy loss has a remarkable impact on the fertility of domestic animals. Buffalo are considered to have low reproductive efficiency also in relation to the seasonality of the species. Most of the embryonic losses occur during the first days after fertilization and during the process of implantation. For that reason it is very important to discriminate, with an early pregnancy diagnosis, pregnant from non pregnant animals particularly when breeding technique such as “out of breeding season mating” or artificial insemination are applied. In the last years, a large polymorphic family of placenta-expressed proteins has been discovered in ruminant species and used for pregnancy diagnosis. Members of this family are named pregnancy-associated glycoproteins (PAG), being synthesized in the mono-and binucleate cells of the ruminant’s trophectoderm. Part of them are released in the maternal blood circulation where they can be assayed by different laboratory techniques. Recently, Barbato et al. (2013), by using biochemical procedures, have isolated from buffalo cotyledons different molecules of the PAG family that have been used to produce antisera for radioimmunoassay (RIA) development. With this method is possible to distinguish between pregnant and no pregnant buffaloes starting from day 25 of gestation. Moreover, the use of PAG assay is useful throughout the gestation period in order to revel the presence of incorrect pregnancy diagnosis and embryonic/fetal mortality. The PAG assay can help veterinarians and breeders in the management of reproduction and represents a powerful tool for investigator involved in studying factors affecting embryo and fetal mortality
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