1,724,084 research outputs found
First person – Kriti Chaplot
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Disease Models & Mechanisms, helping early-career researchers promote themselves alongside their papers. Kriti Chaplot is first author on ‘SOD1 activity threshold and TOR signalling modulate VAP(P58S) aggregation via reactive oxygen species-induced proteasomal degradation in a Drosophila model of amyotrophic lateral sclerosis’, published in DMM. Kriti is a PhD student in the lab of Dr Girish Ratnaparkhi at the Indian Institute of Science Education and Research, Pune, India. Her main research interest is delineating cellular mechanisms that perturb aggregation in neurodegenerative diseases
Regulation of IGF-i Signaling by SOCS3 in Human Coronary Artery Smooth Muscle Cells
No full textPlaque disruption with superimposed thrombosis is the main cause of unstable angina, myocardial infarction and sudden death. Instability of a plaque depends on the balance between release of extracellular matrix by human coronary artery smooth muscle cells (hCASMCs) and cytokines involved in inflammation. Insulin-like growth factor (IGF-i) is one of the most potent inducers of proliferation in hCASMCs, mainly through the activation of phosphatidyl inositol3 (Pl3)-Kinase and mitogen induces protein (MAP) Kinase pathways. Cytokines on the other hand use other pathways such as Jak/STAT (Janus activated kinase/ signal transducers and activators of transcription) to induce their effects. Recent studies have shown the role of suppressors of cytokine signaling, (SOCS) not only in inhibition cytokine signaling, but also in inhibiting growth factor signaling, such as insulin and IGF-i. They act in an auto regulatory negative feedback loop. For the first time I observed a dose dependent increase of SOCS3, which is mostly understood as an inhibitor of STAT3 related pathways, in response to both IGF-i and tumor necrosis factor (TNF)-a, in human coronary artery smooth muscle cells. I also found that SOCS3 inhibits cell proliferation mediated by IGF-i. However, its role in the regulation of smooth muscle cell proliferation in presence of growth factors and cytokines is not yet understood. In addition, underlying cellular and molecular mechanisms of SOCS3 action are unknown. Therefore I proposed an overall hypothesis that SOCS3 involves STAT3 pathway to attenuate IGF-i mediated human coronary artery smooth muscle cell proliferation and atheromatous cytokine TNF-a regulates this effect. I used SOCS3 over-expression and SOCS3 siRNA approaches to confirm the role of SOCS3 in hCASMCs proliferation. Following IGF-i stimulation, cells over-expressing SOCS3 were susceptible to a higher degree of apoptosis and became resistant to IGF-i induced proliferation. SOCS3 siRNA on the other hand, increased IGF-i induced hCASMCs proliferation. Western blot analysis revealed a reduction in IGF-i induced phosphorylation of Akt and Insulin Receptor Substrate-i in cells overexpressed SOCS3. For the first time, my studies showed activation of STAT3 in response to IGF-i. AG490, an inhibitor of STAT3, inhibited IGF-i induced proliferation of hCASMCs as detected by BrdU cell proliferation assay. Over-expression of SOCS3 in hCASMCs also resulted in suppression of IGF-i induced STAT3 activation and nuclear localization as detected by fluorescence microscopy. IGF-l and not TNF-a induced STAT3 phosphorylation; this effect was blocked upon over-expression of SOCS3 in hCASMCs. Inununoprecipitation studies revealed that SOCS3 associated with IGF-i receptor (IGF-iR) upon IGF-i stimulation, indicating its inhibitory role on IGF-i pathway upon association with the receptor. Finally to test the effect of SOCS3 on IGF-i treated hCASMCs in presence of atheromatous cytokine TNF-a, the cells were treated with both IGF-i and TNF-a and SOCS3 expression was found to be completely blocked. Nuclear factor-xB (NF- kB) promoter activity was activated with treatment of TNF-a in contrast to STAT3 activation by IGF-i. Since SOCS3 promoter region has response elements to both these transcription factors, a series of immunoprecipitation experiments revealed that antagonistic interaction of transcription factors STAT3 (activated by IGF-i) and NF-kB (activated by TNF-a) blocked induction of SOCS3 upon adding IGF-i together with TNF- a. Pre-treatment of cells with tyrphostin AG490 resulted in appearance of SOCS3 upon treatment of cells with IGF-i together with TNF-a. These novel findings suggest that SOCS3 expression in hCASMCs regulates the activity of IGF-l signaling. This leads to decreased cell proliferation in hCASMCs which might result in instability of atherosclerotic plaque. Thus, blocking SOCS3 in an atherosclerotic plaque lesion may serve as an important target in regulating IGF-i signaling.ProQuest Traditional Publishing Optionxvi, 208 page
Do food brands enjoy higher brand equity from fortification in the UK? An investigation from Millennials’ perspective towards Kellogg's
No full textPurpose: With the increasing trend of using micronutrient fortification as a tool to boost immunity and fight various deficiency diseases and disorders, it is interesting to explore what it brings for the food manufacturers apart from the increased expenses that stem from the added vitamins and minerals and the modernised technological processes used. This research investigates if the food manufacturers enjoy high customer-based brand equity for their fortified food brands in the UK and if yes how can they optimise it by controlling the different contributing factors. A brand’s brand equity directly translates from the values in the form of brand meanings, advocacy and loyalty that the customers hold for that brand and this study aims to examine how peer influence, willingness to pay and the health labels on the packaging contribute towards increasing these values.
Methodology: The research is focussed on studying the responses from 156 UK millennials (20-40-year-olds), collected through the quantitative method of using a self-administered online questionnaire that are then statistically analysed using SPSS and PROCESS macro for SPSS to deduce important relationships and critical business and marketing implications.
Findings: Results from the detailed analysis indicate that all the three factors significantly contribute towards the customer-based brand equity of fortified food brands in a positive way, with the impact from willingness to pay being the highest, peer influence being the next highest and the health labels being the last. It is also revealed that the effect from the health labels is mediated by peer influence with a positive correlation. Moreover, a critical marketing implication has been uncovered from this study that suggests that the entire packaging mix (pack colours, pictures, brand name, health labels) of a fortified food brand contributes towards consumer decision making as opposed to only health labels as a standalone factor.
Key Words: Fortified foods, functional foods, willingness to pay, Reference Intake, peer influence, health labels, CBBE(Consumer Based Brand Equity
III. Vieux-russe kriti, prés. krĭnju
No full textVaillant André. III. Vieux-russe kriti, prés. krĭnju. In: Revue des études slaves, tome 14, fascicule 1-2, 1934. pp. 78-80
III. Vieux-russe kriti, prés. krĭnju
No full textVaillant André. III. Vieux-russe kriti, prés. krĭnju. In: Revue des études slaves, tome 14, fascicule 1-2, 1934. pp. 78-80
Early Marriage and Health among Women at Midlife: Evidence from India.
No full textJOURNAL OF MARRIAGE AND FAMIL
Early Marriage and Health in Midlife: Evidence of “Weathering” in Indian Women
No full textAmerican Sociological Associatio
Advanced Deep Learning Techniques for Automated Cell Nuclei Segmentation in Biomedical Images
No full textThis dataset includes the U-Net architecture algorithm along with sample data files, which contain both the microscopic images used for analysis and their corresponding masked images. The algorithm was developed and trained using Python, enhancing its ability to perform precise segmentation in biomedical imaging applications
Supplemental Material - Modern Marriage in a Traditional Society: The Influence of College Education on Marriage in India
No full textSupplementary Material for Modern Marriage in a Traditional Society: The Influence of College Education on Marriage in India by Kriti Vikram in Journal of Family Issues.</p
KRITI – the vegetation of Crete database
No full textThe TURBOVEG database on the Cretan covers the South Aegean island of Crete including surrounding islands. It contains plot data on all kinds of woody and non-woody vascular plant vegetation. Bryophytes are included in exceptional cases. It includes relevés from all available sources but chiefly own unpublished relevés. The database provides information on about 90% of the regional flora and of more than 100 endemics. KRITI (GIVD ID EU-GR-001) is a database to be continued. Publications dealing with selected vegetation types or areas have been published
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