1,720,991 research outputs found
Part 1: Preparation of lipid films for phospholipid liposomes v2
No full textThis protocol (like its companion protocol for liposome suspension and extrusion) was original created by Krista Longnecker and Jamie Collins for creating lipsomes to be used in lipid photo-oxidation experiments. The results of these experiments are detailed in Chapter 4 of: Collins, J. R. 2017. The remineralization of marine organic matter by diverse biological and abiotic processes. Ph.D. thesis. Cambridge, Massachusetts: Massachusetts Institute of Technology, 300 pp; doi:10.1575/1912/8721 A manuscript of the chapter is forthcoming. </p
Two-step protocol: Preparation and extrusion of phospholipid liposomes v2
No full textThe protocols in this collection were original created by Krista Longnecker and Jamie Collins for creating lipsomes to be used in lipid photo-oxidation experiments. The results of these experiments are detailed in Chapter 4 of: Collins, J. R. 2017. The remineralization of marine organic matter by diverse biological and abiotic processes. Ph.D. thesis. Cambridge, Massachusetts: Massachusetts Institute of Technology, 300 pp; doi:10.1575/1912/8721 A manuscript of the chapter is forthcoming. </p
Preparation and extrusion of phospholipid liposomes v1
No full textThes protocols were original created by Krista Longnecker and Jamie Collins for creating lipsomes to be used in lipid photo-oxidation experiments. The results of these experiments are detailed in Chapter 4 of: Collins, J. R. 2017. The remineralization of marine organic matter by diverse biological and abiotic processes. Ph.D. thesis. Cambridge, Massachusetts: Massachusetts Institute of Technology, 300 pp; doi:10.1575/1912/8721 A manuscript of the chapter is forthcoming. </p
Preparation of lipid films for phospholipid liposomes v1
No full textThis protocol (like its companion protocol for liposome suspension and extrusion) was original created by Krista Longnecker and Jamie Collins for creating lipsomes to be used in lipid photo-oxidation experiments. The results of these experiments are detailed in Chapter 4 of: Collins, J. R. 2017. The remineralization of marine organic matter by diverse biological and abiotic processes. Ph.D. thesis. Cambridge, Massachusetts: Massachusetts Institute of Technology, 300 pp; doi:10.1575/1912/8721 A manuscript of the chapter is forthcoming. </p
Part 2: Extrusion and suspension of phospholipid liposomes from lipid fims v2
No full textThe protocol can be used to create and extrude phospholipid liposomes. This extrusion protocol roughly follows some guidelines published by the manufacturer. However, we found that the manufacturer's directions were insufficient in several areas. In particular, the manufacturer guidance on initial preparation of lipids was incomplete; 'good' liposomes cannot be prepared directly from dry lipid mixtures. In addition, our protocol provides supplementary guidance for extrusion and suspension of different classes of polar lipids; efficacy of the various matrixes we recommend was largely determined through trial and error. The manufacturer directions (downloaded March 4, 2017) are attached to this protocol as a .pdf document. This protocol was original created by Krista Longnecker and Jamie Collins for creating lipsomes to be used in lipid photo-oxidation experiments. The results of these experiments are detailed in Chapter 4 of: Collins, J. R. 2017. The remineralization of marine organic matter by diverse biological and abiotic processes. Ph.D. thesis. Cambridge, Massachusetts: Massachusetts Institute of Technology, 300 pp; doi:10.1575/1912/8721 A manuscript of the chapter is forthcoming. </p
Extrusion and suspension of phospholipid liposomes from lipid fims v1
No full textThe protocol can be used to create and extrude phospholipid liposomes. This extrusion protocol roughly follows some guidelines published by the manufacturer. However, we found that the manufacturer's directions were insufficient in several areas. In particular, the manufacturer guidance on initial preparation of lipids was incomplete; 'good' liposomes cannot be prepared directly from dry lipid mixtures. In addition, our protocol provides supplementary guidance for extrusion and suspension of different classes of polar lipids; efficacy of the various matrixes we recommend was largely determined through trial and error. The manufacturer directions (downloaded March 4, 2017) are attached to this protocol as a .pdf document. This protocol was original created by Krista Longnecker and Jamie Collins for creating lipsomes to be used in lipid photo-oxidation experiments. The results of these experiments are detailed in Chapter 4 of: Collins, J. R. 2017. The remineralization of marine organic matter by diverse biological and abiotic processes. Ph.D. thesis. Cambridge, Massachusetts: Massachusetts Institute of Technology, 300 pp; doi:10.1575/1912/8721 A manuscript of the chapter is forthcoming. </p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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