1,721,010 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Effect of temperature on functional response of platelet concentrates stored in PVC bags
No full textHaemostatic efficacy of platelet concentrates prepared and stored in locally available PVC triple bags was compared against a Japanese bag. In vitro functional parameters studied included shape change, aggregation and secretion in response to ADP. We have observed remarkable difference in the aggregatory response of platelets stored at slightly varying temperatures. The stimulatory responses of platelets stored with constant agitation at 70 strokes per min and 23+/-2 degrees C, deteriorated drastically by the time platelets were stored for 48 h. Both the rate and the extent of aggregation were affected showing no response to ADP at 72 h. However, when platelets were stored in a BOD incubator, thermostated at 22+/-0.5 degrees C, with continuous horizontal agitation at 70 strokes per min, 50 per cent functional response was retained till 72 h. We also demonstrated fragmentation of platelet membrane during storage. The membrane fragments collected bq. high speed centrifugation, expressed PF3 activity. Shedding of microvesicles indicates alterations at the membrane level that possibly cause functional lesion during storage. Our data suggest the significance of controlling the storage temperature steadily, to get maximum post transfusion efficacy
Cultivation of endothelial progenitor cells on fibrin matrix and layering on dacron/polytetrafluoroethylene vascular grafts
No full textCompletely biological tissue-engineered vascular graft is an upcoming substitute for damaged blood vessel, but its clinical use is currently limited due to poor mechanical strength. Therefore, at present, polymeric small-diameter vascular grafts lined with endothelial cells (ECs) to reduce graft thrombosis may be a more viable option. Successful construction of EC-seeded artificial grafts faces some challenges such as (i) retention of endothelial lining; and (ii) availability of differentiated autologous cells. Biomaterial surfaces that are modified by depositing extracellular matrix (ECM) components may stabilize cells in the lumen against forces of blood flow. Adult stem cells such as endothelial progenitor cells (EPCs) circulate in the blood and they usually attach to the exposed matrix at the injured blood vessel site. Depending on the signaling capabilities of ECM, cells may differentiate into ECs,, and if a similar composition of the matrix is provided in vitro, EPCs isolated from blood might get differentiated and thus autologous cells for tissue engineering may be obtained. In this in vitro study, ECM scaffold consisting of biomolecules such as fibrin, fibronectin, and gelatin along with growth factors is found to have supported differentiation of EPC into EC. Further, the ECM precoated on Dacron and polytetrafluoroethylene is found to have supported the formation of EC monolayer that synthesized nitric oxide, and resisted shear stress. Thus, biomimetic fibrin composite is found to be suitable not only to seed cells on currently available artificial grafts but also to obtain differentiated EC from EPC
Protease action and generation of beta-thromboglobulin-like protein followed by platelet activation
No full textbeta-Thromboglobulin (betaTG) is a platelet specific protein present in the a-granules and secreted into the surrounding medium on cell activation. The sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of platelet releasate after inhibition of metalloproteinases with ethyleneglycol-bis-(beta-aminoethyl ether)N,N'-tetra acetic acid (EGTA) showed disappearance of an 8.0-kDa band. In the absence of the cation chelators, a 48-kDa band disappeared and concurrently, the 8.0-kDa band intensity increased suggesting that the former may be the immediate precursor of the latter. The Western blot stained using specific antibodies, isolated from single-cell clones of hybridoma, against 8.0-kDa protein recognized not only 48- and 8.0-kDa bands but few others too. The data suggest that one or more high molecular weight (HMW) protein is released from alpha-granules and is broken down into smaller fragments after release to form beta-thromboglobulin (beta-TG)-like proteins by the action of metal-dependent proteases. (C) 2002 Published by Elsevier Science Ltd
A SPECTROPHOTOMETRIC METHOD FOR FOLLOWING INITIAL RATE KINETICS OF BLOOD-PLATELET AGGREGATION
No full textA stable matrix for generation of tissue-engineered nonthrombogenic vascular grafts
No full textThe potential of freeze-dried fibrin glue (FG) in combination with growth factor (GF) and gelatin (GEL) is evaluated for use as a matrix for endothelialization of artificial vascular grafts made of polytetrafluoroethylene (PTFE, Teflon) and polyethyleneterephthalate (Dacron). Improved adhesion and proliferation of human umbilical vein endothelial cells are demonstrated on different substrates coated with the FG-GF/FG-GF-GEL mixture, compared with the respective bare surfaces. The strength of adhesion of endothelial cells on the coated matrices was found to be adequate to resist shear stress when monolayers were exposed to forces of flow in an in vitro parallel plate flow chamber. The monolayers maintained physiological nonthrombogenic character as evidenced by in vitro platelet adhesion and response to agonist measurements. Nitric oxide synthesis by cells grown on the study matrices was also found to be normal. Thus, the matrix composition and the coating technique, as presented here, can be easily applied to generate tissue-engineered biomaterials with a nonthrombogenic endothelial cell monolayer for cardiovascular implants. The freeze-drying of the coated matrix ensures prolonged stability and thus the materials can be stored in a ready-to-use state for endothelial cell sodding or seeding
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