1,721,075 research outputs found
Bioinspired Magnetic Nanochains for Medicine
Full text linkSuperparamagnetic iron oxide nanoparticles (SPIONs) have been widely used for medicine, both in therapy and diagnosis. Their guided assembly into anisotropic structures, such as nanochains, has recently opened new research avenues; for instance, targeted drug delivery. Interestingly, magnetic nanochains do occur in nature, and they are thought to be involved in the navigation and geographic orientation of a variety of animals and bacteria, although many open questions on their formation and functioning remain. In this review, we will analyze what is known about the natural formation of magnetic nanochains, as well as the synthetic protocols to produce them in the laboratory, to conclude with an overview of medical applications and an outlook on future opportunities in this exciting research field
Nanocomposite Hydrogels from Nanodiamonds and a Self‐Assembling Tripeptide
Full text linkWe report the successful assembly of a tripeptide in the presence of nanodiamonds (NDs) into nanocomposite hydrogels. The presence of NDs does not hinder peptide self-assembly and gelation, whilst improving the viscoelastic properties of the hydrogels. Gelation kinetics are not affected by NDs, while the elastic moduli of the peptide hydrogels are significantly increased by the NDs. Increased resistance of the gels against applied stress can also be attained depending on the amount of NDs loaded in the nanocomposite. Raman micro-spectroscopy and TEM confirmed the presence of NDs on the surface, and not in the interior, of peptide nanofibers. Peptide-ND non-covalent interactions are also probed by Raman and Fourier-transformed infrared spectroscopies. Overall, this work enables the embedding of NDs into nanocomposite hydrogels formed through the self-assembly of a simple tripeptide at physiological pH, and it provides key insights to open the way for their future applications in biomaterials, for instance exploiting their luminescence and near-infrared responsiveness
Design and fabrication of magnetically responsive nanocarriers for drug delivery
Full text linkMagnetically-assisted delivery of therapeutic agents to the site of interest, which is referred to as magnetic drug targeting, has proven to be a promising strategy in a number of studies. One of the key advantages over other targeting strategies is the possibility to control remotely the distribution and accumulation of the nanocarriers after parenteral administration. However, preparation of effective and robust magnetically responsive nanocarriers based on superparamagnetic iron oxide nanocrystals (SPIONs) still represents a great scientific challenge, since spatial guidance of individual SPIONs is ineffective despite the presence of high magnetic field gradient. A strategy to overcome this issue is the clustering of SPIONs to achieve sufficient magnetic responsiveness. In this mini-review, we address current and future strategies for the design and fabrication of magnetically responsive nanocarriers based on SPIONs for magnetically-targeted drug delivery, including the underlying physical requirements, the possibility of drug loading, and the control of drug release at the targeted site
Dipeptide self-assembly into water-channels and gel biomaterial
Full text linkDipeptides are convenient building blocks for supramolecular gel biomaterials that can be produced on a large scale at low cost and do not persist in the environment. In the case of unprotected sequences, hydrophobicity is a key requirement to enable gelation, with Phe-Phe standing out for its self-assembling ability. Conversely, more hydrophilic sequences such as homochiral dipeptides Phe-Val and Val-Phe neither fibrillate nor gel aqueous buffers and their crystal structures reveal amphipathic layers. In this work, we test emerging rules for the design of self-assembling dipeptides using heterochiral Phe-Val and Val-Phe. Each dipeptide is characterized by H-1- and C-13-NMR, LC-MS, circular dichroism, infrared and Raman spectroscopies, rheology, electron microscopy, and single-crystal X-ray diffraction. In particular, d-Phe-l-Val is the first heterochiral dipeptide to self-assemble into supramolecular water-channels whose cavity is defined by four peptide molecules arranged head-to-tail. This minimalistic sequence is devoid of amyloid character as probed by thioflavin T fluorescence and it displays excellent biocompatibility in vitro. The dataset provided, through comparison with the literature, significantly advances the definition of molecular design rules for minimalistic unprotected dipeptides that self-assemble into water-channels and biocompatible gels, to assist with the future development of supramolecular biomaterials with fine control over nanomorphological features for a variety of applications
A biocatalytic and thermoreversible hydrogel from a histidine-containing tripeptide
Full text linkWe report the first histidine-containing self-assembling tripeptide devoid of capping groups that forms a thermoreversible hydrogel under physiological conditions and catalyses hydrolysis of an ester, providing a minimalist building block for functional soft materials
Single-atom substitution enables supramolecular diversity from dipeptide building blocks
Full text linkDipeptides are popular building blocks for supramolecular gels that do not persist in the environment and may find various applications. In this work, we show that a simple substitution on the aromatic side-chain of phenylalanine with either fluorine or iodine enables supramolecular diversity upon self-assembly at neutral pH, leading to hydrogels or crystals. Each building block is characterized by H-1- and C-13-NMR spectroscopy, LC-MS, circular dichroism, and molecular models. The supramolecular behaviour is monitored with a variety of techniques, including circular dichroism, oscillatory rheology, transmission electron microscopy, attenuated total reflectance Fourier-transformed infrared spectroscopy, visible Raman spectroscopy, synchrotron-radiation single-crystal X-ray diffraction and UV Resonance Raman spectroscopy, allowing key differences to be pinpointed amongst the halogenated analogues
Microwave-Assisted Cyclization of Unprotected Dipeptides in Water to 2,5-Piperazinediones and Self-Assembly Study of Products and Reagents
No full textDipeptides and their cyclized 2,5-piperazinedione (or diketopiperazine, DKP) derivatives are attractive building blocks for supramolecular hydrogels. The Phe-Phe, (p-nitro)-Phe-Phe, and Phe-Val dipeptides and their corresponding DKPs are studied for self-assembly in water. The DKPs were obtained in high yields by microwave-assisted cyclization of the dipeptides in water, demonstrating that use of their methyl ester derivatives as reported in the literature is not necessary for successful cyclization. Single-crystal XRD structures are reported for two DKPs as well as stable hydrogels at neutral pH
Self‐assembling tripeptide forming water‐bound channels and hydrogels
Full text linkD-Ser(tBu)-L-Phe-L-Trp is described as a self-assembling tripeptide that yields nanofibrillar hydrogels at physiological conditions (phosphate buffer at pH 7.4). The peptide is characterized by several spectroscopic methods, such as circular dichroism and fluorescence, oscillatory rheometry, and transmission electron microscopy. Single-crystal X-ray diffraction reveals supramolecular packing into water-bound channels and allows the visualization of the intermolecular interactions holding together peptide stacks
Metal Ions Trigger the Gelation of Cysteine‐Containing Peptide‐Appended Coordination Cages
Full text link: We report a series of coordination cages that incorporate peptide chains at their vertices, prepared through subcomponent self-assembly. Three distinct heterochiral tripeptide subcomponents were incorporated, each exhibiting an L-D-L stereoconfiguration. Through this approach, we prepared and characterized three tetrahedral metal-peptide cages that incorporate thiol and methylthio groups. The gelation of these cages was probed through the binding of additional metal ions, with the metal-peptide cages acting as junctions, owing to the presence of sulfur atoms on the peripheral peptides. Gels were obtained with cages bearing cysteine at the C-terminus. Our strategy for developing functional metal-coordinated supramolecular gels with a modular design may result in the development of materials useful for chemical separations or drug delivery
Luminescent supramolecular hydrogels from a tripeptide and nitrogen-doped carbon nanodots
Full text linkThe combination of different components such as carbon nanostructures and organic gelators into composite nanostructured hydrogels is attracting wide interest for a variety of applications, including sensing and biomaterials. In particular, both supramolecular hydrogels that are formed from unprotected D,L-tripeptides bearing the Phe-Phe motif and nitrogen-doped carbon nanodots (NCNDs) are promising materials for biological use. In this work, they were combined to obtain luminescent, supramolecular hydrogels at physiological conditions. The self-assembly of a tripeptide upon application of a pH trigger was studied in the presence of NCNDs to evaluate effects at the supramolecular level. Luminescent hydrogels were obtained whereby NCND addition allowed the rheological properties to be fine-tuned and led to an overall more homogeneous system composed of thinner fibrils with narrower diameter distributio
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