1,720,958 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Clinical evaluation and study of L-DOPA decarboxylase gene and the novel apoptotic gene, BCL2L12, isoforms in gastric cancer
No full textIn the present study, the expression analysis of both DDC and BCL2L12 mRNA isoforms has been accomplished, in gastric cancer.
Stomach tissue specimens were obtained and the expression levels of DDC and BCL2L12 splice variants were assessed by a regular PCR methodology on a thermal cycler. A highly-sensitive, multiplex Real-Time PCR using TaqMan probes was also developed on a thermal cycler in order to achieve the quantification of the BCL2L12-A mRNA levels. Total protein was extracted from homogenized stomach tissues and the presence of the alt-DDC protein in stomach specimens was investigated by immunoblotting. The specificity of anti alt-DDC antibodies was evaluated using mass spectrometry.
The chemotherapy response of the gastric cancer cell line AGS was analyzed with BCL2L12 and DDC gene expression being compared to the mRNA levels of other apoptosis-related genes.
TaqMan Real-Time PCR analysis depicted an overexpression of BCL2L12-A in cancerous samples. Moreover, a statistically significant relationship was found between the expression of BCL2L12-A in stomach tissues and both the TNM stage of the disease and the grade of the tumor. Survival analysis has also depicted that BCL2L12-A negative patients have survived for a longer period than the BCL2L12-A positive ones. Regular PCR analysis for neuronal and non-neuronal DDC variants showed minor changes between healthy and cancer tissues. The expression of alt-DDC was detected in few stomach tissues and analysis depicted a non statistically significant up-regulation in cancer specimens. Western blotting analysis revealed the presence of the alt-DDC protein in gastric samples, though in varying levels.
The chemosensitivity assay revealed that apoptotic genes and DDC appeared to behave in a diverse manner in the AGS cell line.
The expression profiles of DDC and BCL2L12 in stomach cancer cells suggest their potential application in the discrimination and monitoring of the appropriate curative treatment, in the vein of a more drastic disease treatment.Η παρούσα διδακτορική διατριβή στοχεύει στη μελέτη και την κλινική αξιολόγηση ισομορφών mRNA των γονιδίων DDC και BCL2L12 στον καρκίνο του στομάχου.
Συλλέχθηκαν καρκινικά και φυσιολογικά δείγματα στομάχου και πραγματοποιήθηκε ανάλυση της έκφρασης διαφόρων ισομορφών mRNA των γονιδίων DDC και BCL2L12, με τη μέθοδο της συμβατικής PCR. Αναπτύχθηκε μεθοδολογία πολλαπλής ποσοτικής PCR σε πραγματικό χρόνο (multiplex qReal-Time PCR) με χρήση ανιχνευτών TaqMan, για τον σχετικό ποσοτικό προσδιορισμό των επιπέδων έκφρασης του εναλλακτικού μεταγράφου BCL2L12-Α στα δείγματα ιστού. Σε έναν αριθμό δειγμάτων στομάχου πραγματοποιήθηκε στύπωση Western και ανοσοεντοπισμός της πρωτεΐνης alt-DDC. Η ειδικότητα του ειδικού αντισώματος διερευνήθηκε με φασματομετρία μάζας.
Η απόκριση της καρκινικής κυτταρικής σειράς του στομάχου, AGS, σε χημειοθεραπευτικούς παράγοντες, αναλύθηκε με τον προσδιορισμό των επιπέδων mRNA των γονιδίων DDC και BCL2L12 αλλά και άλλων γονιδίων που σχετίζονται με την απόπτωση.
Τα αποτελέσματα έδειξαν την αυξημένη έκφραση του μετάγραφου BCL2L12-A στους καρκινικούς ιστούς σε σύγκριση με τους παρακείμενους μη καρκινικούς. Ακόμη, παρατηρήθηκε θετική σχέση με το σύστημα TNM και τον βαθμό διαφοροποίησης, ενώ κατά την ανάλυση επιβίωσης οι ασθενείς με αρνητικούς για την έκφραση του BCL2L12-Α καρκίνους επιβίωσαν για μεγαλύτερο χρονικό διάστημα από εκείνους που παρουσιάζουν θετική έκφραση του BCL2L12-Α.
Tα μετάγραφα νευρικού και μη νευρικού τύπου του γονιδίου DDC παρουσίασαν μικρές διαφοροποιήσεις μεταξύ των δειγμάτων ιστού. Το μετάγραφο alt-DDC δε βρέθηκε να εκφράζεται στην πλειονότητα των ιστών, ενώ παρουσίασε αυξημένη έκφραση στα καρκινικά δείγματα. Τα πειράματα ανοσοστύπωσης κατέδειξαν πως τα επίπεδα της πρωτεΐνης alt-DDC στο συγκεκριμένο τύπο ιστού ποικίλλουν και συσχετίζονται με τα αντίστοιχα επίπεδα mRNA.
Η μελέτη της επίδρασης χημειοθεραπευτικών παραγόντων στα καρκινικά κύτταρα στομάχου AGS με τη συμβατική PCR αποκάλυψε σημαντικές μεταβολές στην έκφραση του γονίδιου BCL2L12. Ακόμη μεγαλύτερες μεταβολές παρατηρήθηκαν στα επίπεδα έκφρασης του γονιδίου DDC σχεδόν σε όλα τα φάρμακα που χρησιμοποιήθηκαν. Επιπλέον, οι μεθοδολογίες ποσοτικής PCR πραγματικού χρόνου (qReal-Time PCR), για τα γονίδια DDC και BCL2L12 επιβεβαίωσαν την πλειονότητα των ευρημάτων της μεθόδου RT-PCR. Τα ευρήματα ανέδειξαν τη σημασία των γονιδίων BCL2L12 και DDC στην επιλογή της καταλληλότερης χημειοθεραπευτικής αγωγής με μελλοντικό στόχο την αποτελεσματικότερη αντιμετώπισή της
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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