196,249 research outputs found
Kolin kansallispuiston luontokeskus
No full textDiplomityö perustuu 1997 järjestettyyn Kolin kansallispuiston luontokeskuksen suunnittelukilpailuun osallistuneeseen ehdotukseen.
Suunnitelma koostuu nykyisestä suorakulmaisesta hotellirakennuksesta, erillisestä kaarevaseinäisestä luontokeskuksesta sekä rakennusten väliinsä rajaamasta ulkonäyttelytilasta.
Hotellin tärkein piirre on tilojen avautuminen Pielisen jylhään järvimaisemaan.
Tästä on suunnitelmassa pyritty ottamaan mahdollisimman paljon irti.
Hotellin ulkoasua on pelkistetty ja sovitettu luontokeskuksen puujulkisivuihin.
Puurakenteisen luontokeskuksen kaareva seinä on suunnattu länteen, ilta-aurinkoon.
Seinää reunustaa kapea kattoikkunanauha, jonka kautta auringon valon sävyt ja auringon kierto välittyvät sisätilaan.
Luontokeskus toimii näin eräänlaisena havaintolaitteena, luonnonvalon observatoriona.
Toiminnallisesti ehdotuksen tärkeimmäksi lähtökohdaksi on otettu ajoneuvoliikenteen minimoiminen Kolin lakialueella.
Seurauksena tästä hotellin tilat on järjestelty uudelleen.
Luontokeskuksen kaarevaa seinää myötäilevä kulkuväylä toimii alueen kevyen liikenteen selkärankana yhdistäen sisäänkäynnit sisääntuloaukioon ja pohjoispäädyn terasseihin.
Samalla se on osa Kolin laella kulkevaa vaellusreittiä.
Rakennukset muodostavat ehjän dynaamisen kokonaisuuden, joka toimii kansallispuiston eteistilana rajautuen ympäristöstään selkeästi ihmisen aikaansaamana rakennelmana.
Pinta-alat:.
Luontokeskus yhteensä (hym) 1235 m[2], brutto 1530 m[2], Hotelli yhteensä (hym) 4058 m[2], brutto 5330 m[2]
Kolin Tespiti İçin Moleküler Baskılama Tabanlı Biyosensör Geliştirilmesi
No full textBiosensors are systems that can perform quantitative and/or qualitative analysis of substances in liquid or gasenvironment through their biological recognition sites and transform the acquired data into detectable signals.Biosensors are able to detect physical changes (i.e. as density, mass concentration, etc.) by means of recognitionsites and correlate them with electrical or optical quantities (i.e. current, voltage and impedance). In this study,three molecularly imprinted pencil graphite electrodes with differing numbers of choline recognition sites, at E-1M, E-3 M and E-5 M concentration, were used as electrochemical biosensors. An increase in choline receptorconcentration on the electrode surface was expected to correlate with an increase in PGE surface bound cholineand thus lead to electrical changes. The study was conducted in a three-electrode cell with Ag/AgCl as thereference electrode, platinum wire as the counter electrode and PGE as the working electrode. Cyclicvoltammetry and electrochemical impedance measurements were conducted in 10 mM phosphate buffer solutioncontaining 5mM K3[FeCN6]-3/-4redox pair. As expected, as increasing amount of choline was bound to thecomplementary recognition sites on choline imprinted electrodes, a correlating change in current, voltage andimpedance was observed. The dynamic detection range for choline expanded as the choline concentrationimprinted on the electrodes increased. Using the E-1 M PGE electrode, 72 pM limit of detection, up to 7.2 nMlimit of linearity was attained.Biyolojik sensörün kısaltması olarak kullanılan biyosensörler, maddelerin sıvı ya da gaz ortamda nicel veya nitel tayinini sahip olduğu biyolojik tanıma bölgeleri sayesinde yapabilen ve elde ettiği verileri tespit edilebilir sinyallere çeviren sistemlerdir. Biyosensörler, uygun tanıma bölgeleri aracılığıyla fiziksel değişiklikleri (yoğunluk, kütle, derişim vb.) tespit edebilmekte ve bunları elektriksel veya optik büyüklüklerle (akım, gerilim, empedans vb.) ilişkilendirmektedir. Bu çalışmada, E-1 M, E-3 M ve E-5 M olmak üzere 3 farklı derişimde moleküler baskılanmış, farklı sayıda kolin tanıma bölgelerine sahip, kalem grafit elektrotlar, elektrokimyasal biyosensörler olarak kullanılmıştır. Elektrot yüzeyindeki kolin reseptörü derişimindeki artışın, elektrod yüzeyine bağlı kolindeki artışla ilişkili olması ve dolayısıyla elektriksel değişikliklere yol açması beklenmektedir. Çalışma, üç elektrotlu hücrede, referans elektrot olarak Ag/AgCl, karşı elektrot olarak platin tel ve çalışma elektrotu olarak PGE kullanılarak gerçekleştirilmiştir. Elektrotların açık hücre potansiyeli, dönüşümsel voltametri ve elektrokimyasal empedans ölçümleri, 5mM K3[FeCN6] -3/-4 redoks çifti içeren 10 mM fosfat tampon çözeltisi içerisinde alınmıştır. Çözelti içerisindeki kolinin, kolin baskılanmış elektrodlar üzerindeki tamamlayıcı tanıma alanlarına bağlanmasıyla, elektrodlarda beklenen akım, voltaj ve empedans değişimleri gözlenmiştir. Baskılanan molekül derişiminin artışıyla bağıntılı olarak, tespit aralığında da bir artış gözlenmiştir. Sonuç olarak, E-1 M kolin baskılanan PGE, 7.2 nM-72 pM tespit aralığındaki kolin konsantrasyonunda en yüksek farklılaşmayı göstermiştir
Aula Patientium
No full textVor dem Hintergrund einer Landschaft mit See hat eine nackte Figur den Rücken, auf dem "Patiar ut Potiar" zu lesen ist, zum Betrachter gewendet und den ersten Schritt gewagt, eine dornige Himmelsleiter zu besteigen. In den Wolken thront Christus mit zwei Kronen in Händen, umgeben von Heiligen und EngelnJacob Kolin delin Tugij ; Con. Meyer Tig. fecitEin weiteres Exemplar, ein Zustandsdruck mit Wappen des Abts Augustin II. Reding von Biberegg im linken Vordergrund befindet sich unter derselben Signatur ebenfalls im Bestand der Graphischen SammlungErschienen als Frontispiz in: Iob Christianus, Das ist: Wol-meinende Erinnerung, Der Aller-Heiligsten, Hoch-nothwendigen, Christlichen Tugen Der Gedult: denen vnder so vilfältigen Trübsalen und Beschwärden deß Gemüths, Leibs und Glückes seüfftzenden Christen zu Hilff und Trost : Wie auch Nicht weniger den Predigern, und Seel-Sorgeren zu sonderen Dienst und Kom[m]lichkeiten: In Vier Theil, Mit beigesetzten nothwendigen Registern verfasset, in Druck verfertiget, und verlegt / durch Ioan. Franciscum Suter, der H. Schrifft Licentiatum und Pfarr-Herrn zu Walchwei
Vascular phenotype in angiogenic and non-angiogenic lung non-small cell carcinomas.
No full textWe have previously described a group of non-small cell lung carcinomas without morphological evidence of neo-angiogenesis. In these tumours neoplastic cells fill up the alveoli and the only vessels present appear to belong to the trapped alveolar septa. In the present study we have characterised the phenotype of the vessels present in these non-angiogenic tumours, in normal lung and in angiogenic non-small cell lung carcinomas. The vessels, identified by the expression of CD31, were scored as mature when expressing the epitope LH39 in the basal membrane and as newly formed when expressing alphaVbeta3 on the endothelial cells and/or lacking LH39 expression. In the nine putative non-angiogenic cases examined, the vascular phenotype of all the vessels was the same as that of alveolar vessels in normal lung: LH39 positive and alphaVbeta3 variable or negative. Instead in 104 angiogenic tumours examined, only a minority of vessels (mean 13.1%; range 0--60%) expressed LH39, while alphaVbeta3 (in 45 cases) was strongly expressed on many vessels (mean 55.5%; range 5--90%). We conclude that in putative non-angiogenic tumours the vascular phenotype is that of normal vessels and there is no neo-angiogenesis. This type of cancer may be resistant to some anti-angiogenic therapy and different strategies need to be developed
What motivates interest in attending a familial cancer genetics clinic?
No full textThe motivation of people who seek advice about a family history of cancer was explored in a cross sectional study of new cancer referrals to five regional cancer genetics centres in England: the PACT (patient and clinical team) psychosocial study. One hundred sixty-two people took part. Measures were source of referral, estimated and perceived cancer risk, level of cancer worry, and personal and family-centred reasons for wanting to be seen in clinic. General practitioners referred more people than hospital doctors, and referred a larger proportion of people at low genetic risk of developing cancer. More than half of the participants had been the first to raise the issue of their family history of cancer. Personal motivation for referral is clearly different for those who have had a diagnosis of cancer and for those with children, compared to unaffected and childless people, and is characterised by altruistic concern for other family members rather than a perception of increased personal risk. Men and people from ethnic minorities are very significantly under-represented. Understanding people's motivation may be useful in targeting genetic counselling for people with a family history of cancer. cancer genetics services - familial cancer - familial cancer clinics - genetic counselling - reasons for attendingThis revised version was published online in July 2006 with corrections to the Cover Date
Aula Patientium
No full textVor dem Hintergrund einer Landschaft mit See hat eine nackte Figur den Rücken, auf dem "Patiar ut Potiar" zu lesen ist, zum Betrachter gewendet und den ersten Schritt gewagt, eine dornige Himmelsleiter zu besteigen. In den Wolken thront Christus mit zwei Kronen in Händen, umgeben von Heiligen und Engeln. Im Vordergrund links hält ein Engel das Wappen des Abts Augustin II. Reding von BibereggJacob Kolin delin Tugij ; Con. Meyer Tig. fecitEin weiteres Exemplar, ein Zustandsdruck ohne das Wappen des Abts Augustin II. Reding von Biberegg im linken Vordergrund, befindet sich unter derselben Signatur ebenfalls im Bestand der Graphischen SammlungErschienen als Frontispiz in: Iob Christianus, Das ist: Wol-meinende Erinnerung, Der Aller-Heiligsten, Hoch-nothwendigen, Christlichen Tugen Der Gedult: denen vnder so vilfältigen Trübsalen und Beschwärden deß Gemüths, Leibs und Glückes seüfftzenden Christen zu Hilff und Trost: Wie auch Nicht weniger den Predigern, und Seel-Sorgeren zu sonderen Dienst und Kom[m]lichkeiten: In Vier Theil, Mit beigesetzten nothwendigen Registern verfasset, in Druck verfertiget, und verlegt / durch Ioan. Franciscum Suter, der H. Schrifft Licentiatum und Pfarr-Herrn zu Walchwei
Risk factors for cataract subtypes waterclefts and retrodots: two case-control studies.
No full textUNLABELLED: Waterclefts and retrodots are independently associated with visual impairment, yet a review identified no data on risk factors. PURPOSE: To investigate risk factors for these two human lens cataract subtypes. METHOD: Two nested case-control studies: The host study comprised 1078 subjects (55 years) attending the Somerset and Avon Eye Study (SAES). In total, 197 watercleft cases (Oxford grade 0.2 in either eye) and 199 retrodot cases (Oxford grade 1.0 in either eye) were individually age/gender matched to controls. Detailed ophthalmic and potential risk factor data were collected, including body mass index (BMI), smoking, alcohol, diabetes, hypertension, analgesics, vitamin supplementation, nutrition, sunlight exposure, dehydration, hormonal (women), blood lipids, glucose, urea, creatinine, uric acid, and vitamin levels. RESULTS: For waterclefts, univariable analysis identified BMI, alcohol intake, vitamin status, sunlight, urea, creatinine, and uric acid as possible risk factors. Multivariable analysis identified two independent associations. Total number of 'any' analgesics in the previous year: adjusted P<0.01 (U-shaped risk profile, unadjusted high vs medium use (=reference) OR 2.39, 95% CI 1.35-4.26 with medium use vs none (=reference) OR 0.43, 95% CI 0.26-0.72); total sunlight: adjusted P=0.03 (unadjusted highest exposure vs lowest (=reference) OR 3.25, 95% CI 1.11-9.50). For retrodots, univariable analysis identified alcohol, HRT, and lipids. Multivariable analysis identified two independent associations. Mean number of alcohol units consumed per month, adjusted P=0.02 and HDL cholesterol levels, adjusted P=0.02 (unadjusted ORs NS both). CONCLUSION: This is the first available published information on risk factors for the human cataractous lens features waterclefts and retrodots
Sıçanda deneysel miyokardiyal iskemi - reperfüzyon modelinde CDP- kolin ve metabolitlerinin etkileri
No full textSitidin-5'-difosfatkolin (CDP-kolin) endojen olarak sentezlenen bir mononükleotiddir. Aynı zamanda, serebral iskemik durumlarda bir ilaç olarak kullanılmaktadır. Bu çalışmada, CDP-kolin'in sıçanlarda miyokardiyal iskemireperfüzyon hasarı gelişimine karşı koruyucu etkileri olup olmadığı araştırıldı. Anestezi altındaki sıçanların sol ana koroner arteri 7 dakika süre ile bağlanarak ve takiben 7 dakika süresince arterdeki bağ serbest hale getirilerek kısa dönem iskemi-reperfüzyon oluşturuldu. Reperfüzyon kaynaklı ventriküler taşikardi (VT), ventriküler fibrilasyon (VF), kan basıncı değişiklikleri ve ölüm oranları değerlendirildi. Kontrol grubundaki sıçanlarda VT, VF ve ölüm oranları sırasıyla %85, %62 ve %54 olarak gözlendi. lntravenöz (i.v.) yol ile verilen CDP-kolin (100, 250 ve 500 mg/kg), kan basıncında kısa süreli bir artışa neden oldu. 250 ve 500 mg/kg dozlarında verilen CDP-kolin, VT ve VF insidanslarını azaltırken iskemi-reperfüzyona bağlı ölümü tamamen engelledi. 250 mg/kg CDP-kolin'e eşdeğer dozda kolin (64 mg/kg; i.v.) veya sitidin (111 mg/kg; i.v.), iskemi-reperfüzyona bağlı VT oluşumunu büyük oranda azaltırken, VF gelişimini ve ölümü tamamen bloke etti. Kolin ve sitidin, kan basıncını düşürdü. Anestezi altındaki sıçanların sol ana koroner arteri 30 dakika süre ile bağlanarak ve takiben 3 saat süresince arterdeki bağ serbest hale getirilerek uzun dönem iskemi-reperfüzyon oluşturuldu. İskemi-reperfüzyona bağlı nekroz miktarı ölçüldü. CDP-kolin, 250 mg/kg dozunda, nekroz miktarını ve nekroz miktarının risk bölgesine oranını, kontrol grubuna göre, anlamlı olarak azalttı. Bu çalışmadan elde edilen bulgular, CDP-kolin'in miyokardiyal iskemireperfüzyon hasarında kardiyoprotektif etkiye sahip olduğunu göstermektedir. Kısa dönem iskemi-reperfüzyon hasarında hem kolin hem de sitidin, CDP-kolin'in bu koruyucu etkisinde rol oynuyor gözükmektedir.Cytidine-5'-diphosphocholine (CDP-choline) is an endogenously synthesized mononucleotide. lt is, also, a drug used for the treatment of cerebral ischemic situations. In the present study we aimed to determine whether CDP-choline can have any protective effect against the outcome of the myocardial ischemia-reperfusion injury in rats. Short term ischemia-reperfusion was produced in anaesthetized rats by ligature of the left main coronary artery for 7 min followed by reperfusion period for the next 7 min. Reperfusion-induced ventricular tachycardia (VT), ventricular fibrillation (VF), changes in arterial pressure and lethality rate were evaluated. In control rats, VT, VF and lethality were observed in %85, %62 and %54 animals, respectively. Intravenous (i.v.) CDP-choline (100, 250 and 500 mg/kg) produced short term increase in blood pressure. At the dose of 250 and 500 mg/kg, CDP-choline reduced the incidence of VT, VF and lethality when injected in ischemic period. CDP-choline, at the dose of 250 mg/kg and 500 mg/kg, completely prevented the death. Choline (64 mg/kg; i.v.) or cytidine (111 mg/kg; i.v.) administration at the dose of equimolar to 250 mg/kg CDP-choline, greatly attenuated the incidence of VT and abolished the occurence of VF and death. Blood pressure of rats was decreased in choline or cytidine injected rats. Long term ischemia-reperfusion was produced in anaesthetized rats by ligature of the left main coronary artery for 30 min followed by reperfusion period for the next 3 h. lschemia-reperfusion induced infarct size was measured. Compared to the control group, CDP-choline, at the dose of 250 mg/kg, reduced infarct size induced by ischemia-reperfusion and infarct size/risk zone. Results show that CDP-choline exerts cardioprotective effect in myocardial ischemia-reperfusion injury. Both cheline and cytidine seem to be involved in CDP-choline's effect in short term ischemia-reperfusion
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