184,724 research outputs found
Architecture of a transcribing-translating expressome
No full textCoupling transcription and translation
In bacteria, the transcription of DNA into mRNA by RNA polymerase is coupled to the translation of that mRNA into protein by the ribosome. How this coupling is achieved has been unclear. Kohler
et al.
show that RNA polymerase and the ribosome from
Escherichia coli
can form a so-called expressome complex. Electron microscopy structural analysis, together with functional experiments, revealed details of the coupled complex. The coupling could allow translation to prevent transcriptional pausing, backtracking, and termination.
Science
, this issue p.
194
</jats:p
Viggo Kohler and family
No full textPhotograph shows a composite picture of Viggo Kohler and his family. Standing: Lucile Kohler Gruy (adopted daughter of Viggo); seated (l to r): Joseph Gruy (husband of Lucile) with Viggo Gruy in his lap; Joe Gruy, Jr. (painted into the picture 1935-1936); Viggo Kohler with Dagmar Gruy (Cole) on his lap
Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6
No full textDilcher M, Kohler B, Fischer von Mollard G. Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. JOURNAL OF BIOLOGICAL CHEMISTRY. 2001;276(37):34537-34544.SNARE proteins are required for fusion of transport vesicles with target membranes. Previously, we found that the yeast Q-SNARE Vti1p is involved in transport to the cis-Golgi, to the prevacuole/late endosome, and to the vacuole. Here we identified a previously uncharacterized gene, VTS1, and the R-SNARE YKT6 both as multicopy and as low copy suppressors of the growth and vacuolar transport defect in uti1-2 cells. Ykt6p was known to function in retrograde traffic to the cis-Golgi and homotypic vacuolar fusion. We found that VTI1 and YKT6 also interacted in traffic to the prevacuole and vacuole, indicating that these SNARE complexes contain Ykt6p, Vti1p, plus Pep12p and Ykt6p, Vti1p, Vam3p, plus Vam7p, respectively. As Ykt6p was required for several transport steps, R-SNAREs cannot be the sole determinants of specificity. To study the role of the 0 layer in the SNARE motif, we introduced the mutations vti1-Q158R and ykt6-R165Q. SNARE complexes to which Ykt6p contributed a fourth glutamine residue in the 0 layer were nonfunctional, suggesting an essential function for arginine in the 0 layer of these complexes. vti1-Q158R cells had severe defects in several transport steps, indicating that the second arginine in the 0 layer interfered with function
Kohler Buys 303 Garage
No full textW. R. Kohler of LaHarpe purchased 303 garage on South street, previously owned by H. L. Sayers and C. E. Shapel. Shapel employed George Sweatt as an autorepair man, the occupation he listed on his WWI draft card
Bibliotheca geographica Palaestinae. Chronologisches Verzeichniss der auf die Geographie des heiligen Landes bezüglichen Literatur, von 333 bis 1878, und Versuch einer Cartographie, par R. Röhricht.
No full textKohler Charles. Bibliotheca geographica Palaestinae. Chronologisches Verzeichniss der auf die Geographie des heiligen Landes bezüglichen Literatur, von 333 bis 1878, und Versuch einer Cartographie, par R. Röhricht.. In: Bibliothèque de l'école des chartes. 1891, tome 52. pp. 451-452
Bibliotheca geographica Palaestinae. Chronologisches Verzeichniss der auf die Geographie des heiligen Landes bezüglichen Literatur, von 333 bis 1878, und Versuch einer Cartographie, par R. Röhricht.
No full textKohler Charles. Bibliotheca geographica Palaestinae. Chronologisches Verzeichniss der auf die Geographie des heiligen Landes bezüglichen Literatur, von 333 bis 1878, und Versuch einer Cartographie, par R. Röhricht.. In: Bibliothèque de l'école des chartes. 1891, tome 52. pp. 451-452
L. Bourrat, J. Dechaume, R. Gallavardin, P.-F. Girard, C. Kohler, R. Pellet, L, Thévenin, L. Vérel, L'Enfance irrégulière. Psychologie clinique
No full textFauville Arthur. L. Bourrat, J. Dechaume, R. Gallavardin, P.-F. Girard, C. Kohler, R. Pellet, L, Thévenin, L. Vérel, L'Enfance irrégulière. Psychologie clinique. In: Revue Philosophique de Louvain. Troisième série, tome 44, n°3, 1946. pp. 461-462
FURTHER EPR AND ENDOR STUDIES OF DIPHENYLMETHYLENE
No full textR. W. Brandon, G. L. Closs, C. E. Davoust, C. A. Hutchison, B, E. Kohler, and R. Silbey, J. Chem. Phys. 43, 2006 (1965). C. A. Hutchison and B. E. Kohler, J. Chem. Phys. 51, 3327 (1969).Author Institution: Department of Chemistry, Harvard UniversityThe original magnetic resonance studies of dipheny Imethylene in single crystal have been extended. EPR and ENDOR experiments on diphenylmethylene in diphenylethylene, benzophenone, and 4-bromopheoyl ether will be discussed. The orientation and conformation of this ground state triplet in each of these hosts will be described, with a view toward the understanding of the differences in the optical spectrum in each host
Mississippi State University Research / Demonstration Module
No full textA final report on the Mississippi State Module Planning Grant prepared by E. T. Kohler and R. C. Maxson in September of 1968
Kohler, Jean-Marie. Les migrations des Mosi de l'Ouest
No full textPageard R. Kohler, Jean-Marie. Les migrations des Mosi de l'Ouest . In: Journal de la Société des Africanistes, 1973, tome 43, fascicule 2. pp. 280-281
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