20 research outputs found
Serum vitamin B-12 in children presenting with vasovagal syncope
No full textBackground and Objectives: The present study aims to determine the serum vitamin B-12 in children presenting with vasovagal syncope. Methods and Study Design: This is a prospective review of 160 children presenting with vasovagal syncope. Subgroup analysis was done based on the results of head up tilt test. Results: Head up tilt test gave positive results in 80 children and yielded negative results in the remaining 80 children. The tilt test positive children had significantly lower thyroid stimulating hormone concentrations (p=0.06), total iron binding capacity (p=0.04) and serum vitamin B-12 (p=0.01). The prevalence of vitamin B-12 deficiency was significantly higher in the tilt positive group (80% vs 52.5%, p=0.001). Out of 80 children with positive tilt test, 8 children (10%) showed cardioinhibitory response, 22 children (27.5%) demonstrated a vasodepressor response, 24 children (30%) displayed mixed response and 26 children (32.5%) had the postural orthostatic tachycardia syndrome. Erythrocyte sedimentation rate was significantly lower in the mixed response group than in the vasodepressor group (6.2 +/- 0.8 mm/h vs 14.3 +/- 2.5 mm/h, p=0.001). Serum vitamin B-12 was significantly lower in the postural orthostatic tachycardia syndrome (POTS) group than in the vasodepressor group (240.8 +/- 38.2 pg/mL vs 392.7 +/- 27.1 pg/mL, p=0.001). The prevalence of vitamin B-12 deficiency was significantly higher in the POTS group than in the vasodepressor group (92.3% vs 45.5%, p=0.001). Conclusions: Vitamin B-12 deficiency causes reduction in myelinization, deceleration in nerve conduction and elevation in serum concentrations of noradrenaline. These factors may contribute to the impairment of autonomic functions which are involved in the pathogenesis of vasovagal syncope
Effects of hemodialysis period on levels of blood trace elements and oxidative stress
No full textAs a treatment method for chronic renal failure (CRF), hemodialysis (HD) alters inorganic components containing trace elements. It was shown that decreased renal function is accompanied by insufficient antioxidant systems and/or increased free oxygen radicals. In this study, we aimed to investigate the effects of HD on trace element levels and oxidative stress markers. We included 111 CRF patients on HD treatment three times a week and 24 healthy controls. Patients were divided into four groups according to HD duration. Plasma malondialdehyde (MDA), protein carbonyls and total sulfhydryl (-SH) levels, zinc (Zn), copper (Cu), and magnesium (Mg) levels, and erythrocyte superoxide dismutase (SOD) activity were measured from blood taken from patients before HD. SH levels and SOD activity in all groups were significantly lower than those in the control group (p < 0.001). All groups had significantly higher plasma MDA levels than did controls (p < 0.001). Whereas there was no significant difference in -SH levels and SOD activity between groups, increased periods of HD were associated with increases in MDA. MDA levels of the third and fourth groups were significantly higher than in the first and second groups (p < 0.05, p < 0.001, respectively). There was no significant difference of Zn, Cu, Mg, and protein carbonyl levels in and between all groups. However, plasma Cu levels and MDA concentrations were correlated (r = 0.26, p < 0.01). Prolonged exposure to HD can cause increased oxidative damage but has no effect on trace element concentration
Dietary Fructose Activates Insulin Signaling and Inflammation in Adipose Tissue: Modulatory Role of Resveratrol
No full textThe effects of high-fructose diet on adipose tissue insulin signaling and inflammatory process have been poorly documented. In this study, we examined the influences of long-term fructose intake and resveratrol supplementation on the expression of genes involved in insulin signaling and the levels of inflammatory cytokines and sex hormones in the white adipose tissues of male and female rats. Consumption of high-fructose diet for 24 weeks increased the expression of genes involved in insulin signaling including IR, IRS-1, IRS-2, Akt, PI3K, eNOS, mTOR, and PPAR , despite induction of proinflammatory markers, iNOS, TNF , IL-1 , IL-18, MDA, and ALT, as well as anti-inflammatory factors, IL-10 and Nrf2 in adipose tissues from males and females. Total and free testosterone concentrations of adipose tissues were impaired in males but increased in females, although there were no changes in their blood levels. Resveratrol supplementation markedly restored the levels of MDA, IL6, IL-10, and IL-18, as well as iNOS, Nrf2, and PI3K mRNA, in adipose tissues of both genders. Dietary fructose activates both insulin signaling and inflammatory pathway in the adipose tissues of male and female rats proposing no correlation between the tissue insulin signaling and inflammation. Resveratrol has partly modulatory effects on fructose-induced changes
Resveratrol improves hepatic insulin signaling and reduces the inflammatory response in streptozotocin-induced diabetes
No full textWOS:000360511100008PubMed:26071184Diabetes mellitus is a heterogeneous metabolic disorder essentially characterized by deficiency of insulin secretion, insulin receptor or post-receptor events. This study aims to investigate the effects of resveratrol administration on the metabolic characteristics, hepatic functions, histopathological features and insulin signaling pathway components in streptozotocin induced diabetes. Male Wistar rats were randomly divided into four groups: (1) control/vehicle; (2) control/20 mg/kg resveratrol; (3) diabetic/vehicle; and (4) diabetic/20 mg/kg resveratrol. Histopathological examinations were carried out to reveal hepatic tissue damage and inflammation. In addition to hepatic glucose, lipid, insulin, ALT, AST, resistin and XOD contents, gene and protein expressions of insulin signaling pathway components such as insulin RI3, IRS-1, IRS-2, eNOS, PI3K, Alct, and FOX03a were analyzed by qRT-PCR and Western blot. The rats in the diabetes group had significantly lower terminal body weight and hepatic insulin level, but significantly higher hepatic glucose, total cholesterol, triglyceride and resistin concentrations. Diabetes triggered the inflammatory process in the liver tissues that was evidenced by histopathological deformations and increase in the hepatic ALT and AST levels. Hepatic inflammation was considerably associated with insulin signaling pathway ever since a significant down-regulation of insulin signaling components; IRS-1, IRS-2, PI3K, Alct and mTOR have been identified in the diabetic group. To some extent, resveratrol treatment reversed the diabetes-induced changes in the liver tissues. Taken together, resveratrol partly improved hepatic dysfunction induced by diabetes. This may be due to the healing activity of resveratrol on insulin signaling pathway, resistin levels and hepatic glucose-lipid contents. 0 2015 Elsevier B.V. All rights reserved.TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [3501/112T159]This study was supported by grants from TUBITAK Research Fund (3501/112T159)
Monitoring of pre-competition dehydration- induced skeletal muscle damage and inflammation levels among elite wrestlers
No full textBACKGROUND: Weight loss practice before competitions is a common method in weight sports. OBJECTIVE: The current study aimed to monitor Plasma Osmolarity (P-Osm), skeletal muscle damage and inflammation levels induced by pre-competition dehydration among elite wrestlers. METHODS: Twenty-four male elite wrestlers participated in the study. A survey information form that assessed pre-competition weight loss practices were administered to the wrestlers. Additionally, Total Creatine Kinase (CK), Lactate Dehydrogenase (LDH), Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), C-reactive Protein (C-RP) and P-Osm, analyses were evaluated by a specialist at the biochemical laboratory. P-Osm level for euhydration is 290, then dehydration occurs. The wrestlers were divided into two groups according to P-Osm levels as weight loss and non-weight loss group. RESULTS: There were differences in the pre and post-test P-Osm levels of weight loss group. Similarly; it was identified that there were also statistically significant differences in terms of ALT, LDH and CK levels of weight loss group between pre and post-test. CONCLUSIONS: It was seen that P-Osm and levels of all indicators of skeletal muscle damage were higher than non-weight loss group. However, it was found that there were not any differences in terms of C-RP levels.Afyon Kocatepe University, Scientific Research Coordination Department [16.KARIYER.84]The authors wish to acknowledge all the national team wrestlers and coaches. They also thank Sefa Demir and Emel Kaya who are working as nurses in the Afyon Kocatepe University Hospital for their technical assistance in taking the blood samples. Abstract of this study was presented as an oral presentation at 2nd International Conference on the Changing World and Social Research, 14-16 Oct 2016, Barcelona-Spain. Finally, the study was financially funded by Afyon Kocatepe University, Scientific Research Coordination Department with protocol number 16.KARIYER.84
The effects of general and spinal anesthesia on systemic inflammatory response in patients undergoing total knee arthroplasty
No full textObjectives: This study aims to compare the systemic inflammatory responses (SIRs) developing after total knee arthroplasty (TKA) performed under general or spinal anesthesia. Patients and methods: This prospective study included 40 patients (8 males, 32 females; mean age 67.15 +/- 9.27 years; range 51 to 89 years) who underwent TKA in our clinic between February 2014 and July 2014. Patients were grouped to receive general (group 1, n= 20) or spinal anesthesia (group 2, n= 20). Levels of pro-inflammatory markers [Interleukin-6 (IL-6), IL-8, IL-1 beta, tumor necrosis factor-alpha (TNF-alpha) and C-reactive protein (CRP)] were studied from the venous blood samples obtained immediately before induction to anesthesia (T-1), immediately after closure of the operative wound (T-2), and at 24 hours postoperatively (T-3). Results: In both groups, levels of CRP and IL-6 were significantly increased at T-3 compared to those achieved at T-1. Changes in the levels of TNF-alpha in both groups were similar. There were no significant differences between the groups in terms of the changes within the levels of the studied markers at the respective time intervals. Conclusion: According to our study results, SIRs developing after TKA performed under general or spinal anesthesia are similar.Scientific Research Projects Coordination Unit of Afyon Kocatepe University, TurkeyThis study was supported by the Scientific Research Projects Coordination Unit of Afyon Kocatepe University, Turkey
A comparison of miRNAs with proinflammatory cytokines and procalcitonin of sepsis in the intensive care unit A comparison of miRNAs with proinflammatory cytokines and procalcitonin
No full textAim: The development of sepsis, the low efficacy of antibiotics used, long-term antibiotic use, and the development of resistance to antibiotics are significant problems in patients in intensive care units. The use of biological markers is promising for the diagnosis and treatment of sepsis. In this study, proinflammatory cytokines, procalcitonin and four miRNA expressions were analyzed in a time-dependent manner in a patient group and control group, and the correlations between them were examined
Dietary fructose activates ınsulin signaling and ınflammation in adipose tissue: modulatory role of resveratrol
No full textWOS:000373088900001PubMed:27066503The effects of high-fructose diet on adipose tissue insulin signaling and inflammatory process have been poorly documented. In this study, we examined the influences of long-term fructose intake and resveratrol supplementation on the expression of genes involved in insulin signaling and the levels of inflammatory cytokines and sex hormones in the white adipose tissues of male and female rats. Consumption of high-fructose diet for 24 weeks increased the expression of genes involved in insulin signaling including IR, IRS-1, IRS-2, Akt, PI3K, eNOS, mTOR, and PPAR gamma, despite induction of proinflammatory markers, iNOS, TNF alpha, IL-1 beta, IL-18, MDA, and ALT, as well as anti-inflammatory factors, IL-10 and Nrf2 in adipose tissues from males and females. Total and free testosterone concentrations of adipose tissues were impaired in males but increased in females, although there were no changes in their blood levels. Resveratrol supplementation markedly restored the levels of MDA, IL6, IL-10, and IL-18, as well as iNOS, Nrf2, and PI3K mRNA, in adipose tissues of both genders. Dietary fructose activates both insulin signaling and inflammatory pathway in the adipose tissues of male and female rats proposing no correlation between the tissue insulin signaling and inflammation. Resveratrol has partly modulatory effects on fructose-induced changes.Gazi University Research FundGazi University [BAP 02/2010-27]This study was supported by grants from Gazi University Research Fund (BAP 02/2010-27)
The role of ADAMTS-2, collagen type-1, TIMP-3 and papilin levels of uterosacral and cardinal ligaments in the etiopathogenesis of pelvic organ prolapse among women without stress urinary incontinence
No full textObjective(s): To investigate the potential role of 'a disintegrin-like and metalloproteinase with thrombospondin type motifs-2 (ADAMTS-2), collagen type-1, tissue inhibitor of metalloproteinases-3 (TIMP-3) and papilin' levels in the uterosacral ligament (USL) and cardinal ligament (CL) of the uterus on the etiopathogenesis of pelvic organ prolapse (POP) among postmenopausal women without stress urinary incontinence (SUI). Study design: A total of 45 postmenopausal women, 22 diagnosed as POP stage III-IV and 23 age- and body mass index (BMI)-matched controls referred for hysterectomy due to POP or benign gynecological disease, respectively, were recruited prospectively for our study. The biopsies of the USL and CL were obtained during hysterectomy. ADAMTS-2, collagen type-1, TIMP-3 and papilin levels were determined by enzyme-linked immunosorbent assay (ELISA) method after tissue homogenization. We excluded patients who smoked or presented with SUI. Results: There were no differences in terms of demographic features including age, BMI, obesity, duration of menopause, gravidity, parity, delivery modes and family history for POP between the POP and non-POP groups. Significant differences in the levels of ADAMTS-2, collagen type-1, TIMP-3 and papilin of USL were noted among the groups. Females with POP had lower levels of ADAMTS-2, collagen type-1, TIMP-3 and papilin in the USL compared to non-POP females. All investigated markers in the CL were also decreased in the POP group, but this relationship was not statistically significant. When age, duration of menopause, gravidity, parity and obesity were taken as covariates, only the USL papilin levels were negatively predictive for the development of POP. Conclusion(s): ADAMTS-2, collagen type-1, TIMP-3 and papilin levels of the USL play essential roles in the etiopathogenesis of POP among postmenopausal women without SUI. Moreover, significantly decreased USL papilin levels in females with POP suggest the importance of the USL and the impact of papilin on the development of POP. (C) 2018 Elsevier B.V. All rights reserved
Dietary Fructose Activates Insulin Signaling and Inflammation in Adipose Tissue: Modulatory Role of Resveratrol
No full textThe effects of high-fructose diet on adipose tissue insulin signaling
and inflammatory process have been poorly documented. In this study, we
examined the influences of long-term fructose intake and resveratrol
supplementation on the expression of genes involved in insulin signaling
and the levels of inflammatory cytokines and sex hormones in the white
adipose tissues of male and female rats. Consumption of high-fructose
diet for 24 weeks increased the expression of genes involved in insulin
signaling including IR, IRS-1, IRS-2, Akt, PI3K, eNOS, mTOR, and PPAR
gamma, despite induction of proinflammatory markers, iNOS, TNF alpha,
IL-1 beta, IL-18, MDA, and ALT, as well as anti-inflammatory factors,
IL-10 and Nrf2 in adipose tissues from males and females. Total and free
testosterone concentrations of adipose tissues were impaired in males
but increased in females, although there were no changes in their blood
levels. Resveratrol supplementation markedly restored the levels of MDA,
IL6, IL-10, and IL-18, as well as iNOS, Nrf2, and PI3K mRNA, in adipose
tissues of both genders. Dietary fructose activates both insulin
signaling and inflammatory pathway in the adipose tissues of male and
female rats proposing no correlation between the tissue insulin
signaling and inflammation. Resveratrol has partly modulatory effects on
fructose-induced changes
