1,721,607 research outputs found
Flow cytometry characterization of Het-F and KO-M mice.
No full text(A) Cell lineage frequencies for BM, spleen and PB from young and aged, KO-M and WT-M mice were determined by flow cytometry for myeloid cells, B-cells, T-cells, NK cells and erythroid precursors. There was a significant decrease in B220+ B cells in the spleen of young KO-M mice as compared to WT-M mice. (B) Cell lineage frequencies for BM, spleen and PB from young and aged, Het-F and WT-F mice were determined by flow cytometry for myeloid cells, B cells, T cells, NK cells and erythroid precursors. There were no significant differences observed in these compartments. Error bars represented mean ± s.d. *pt-test. (PDF)</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Characterization of the Het-F and KO-M mice.
No full text(A) CBCs for the young and aged KO-M mice versus WT-M mice showed no significant differences, except in aged mice which had an increase in MCV. (B) CBCs for the young and aged Het-F mice versus WT-F mice showed no significant differences. (C) Splenomegaly was present in young KO-F mice (0.13 g in KO-F mice versus 0.06 g in WT-F mice, p = 0.0001) and aged KO-F mice (0.212 g in KO-F mice versus 0.085 g in WT-F mice, p = 0.0062). We also observed mild splenomegaly in young KO-M mice (0.11 g in KO-M mice versus 0.07 g in WT-M mice, p = 0.0102), but no significant differences between other matched cohorts. (D) Histopathologic examination of BM and spleen by H&E staining. BM sections showed moderately to markedly increased numbers of myeloid cells and decreased numbers of erythroid cells from young to aged KO-F mice. The myeloid to erythroid ratio was typically elevated, up to 20:1 in some fields. Emperipolesis was often noted. Megakaryocytes were present in expected to increased numbers. Sections of spleen from KO-F mice showed extramedullary erythropoiesis and granulopoiesis that increased with age. Representative images (40x magnification) including small fields (100x magnification) are shown. Error bars represented mean ± s.d. *pt-test. (PDF)</p
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
H-NS-dependent regulation of flagellar synthesis is mediated by a LysR family protein
No full textH-NS regulates the flagellar master operon (flhDC) and thus is necessary for flagellation of Escherichia coli. However, the molecular mechanism of its regulation has remained unknown. Genetic screening of a transposon insertion abolishing the H-NS effect revealed a previously unidentified gene, named hdfR, encoding a LysR family protein. Binding of purified HdfR to the flhDC promoter was demonstrated by a DNA mobility shift assay, indicating that HdfR is a transcriptional regulator for the flagellar master operon. Furthermore, the expression of the hdfR gene was shown to be negatively regulated by H-NS
Two novel flagellar components and H-NS are involved in the motor function of Escherichia coli
No full textA mutation in H-NS results in non-flagellation of Escherichia coli due to a reduced expression of the flhDC master operon. We found that the hns-negative strain restored its flagellation in the presence of flhDC, although the resulting strain was still non-motile. Since the intracelluar levels of motor components MotA, MotB, and FliG in the Delta hns strain were unaltered, the non-motility indicates that H-NS affects flagellar function as well as biogenesis. We obtained an insertion in ycgR, a putative gene encoding a protein of 244 amino acid residues, which suppresses the motility defect of hns-deficient cells. The abnormally low swimming speed of hns mutant cells was fully restored by an insertion in ycgR, as assessed with computer-assisted motion analysis. A similar suppressor phenotype was observed with a multicopy expression of yhjH, a putative gene encoding a polypeptide of 256 amino acid residues. Since the flagella of most hns-deficient cells were not rotating, except a few with reduced speed, the suppression appears to increase the number of rotating flagella as observed with tethered bacteria. The ycgR and yhjH genes contain the consensus sequence found among the class III promoters of the flagellar regulon, and their expression monitored with a lacZ fusion requires FlhDC. These findings suggest that ycgR and yhjH, together with H-NS, are involved in the motor function and constitute new members of the flagellar regulon. (C) 2000 Academic Press
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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