867 research outputs found
Donor Variability and Seeding Density Shape NK-Cell Proliferation and Surface Receptor Expression: Insights from an Integrated Phenotypic and Genetic Analysis
No full textNatural killer (NK) cells are promising candidates for adoptive immunotherapy, but their clinical application requires standardized expansion protocols that yield functional cells in sufficient numbers. This study examined how initial seeding density and donor-intrinsic variability affect NK cell proliferation and receptor phenotype during in vitro expansion in a G-Rex® 24-well plate under IL-2 stimulation. NK cells from healthy donors were analyzed longitudinally by flow cytometry, and targeted SNP sequencing of selected receptor genes (IL2RA, IL2RB, FCGR3A, NCR1, KLRK1, and ICAM-1) was performed to assess potential genetic contributions. A seeding density of 2.0 × 106 cells/cm2 promoted high expansion rates and favorable expression of activating receptors including CD16a, NKp46, and NKG2D. Nonetheless, marked inter-donor differences were observed. Some donors exhibited impaired proliferation and aberrant receptor expression, possibly associated with high-priority SNPs and distinct haplotype structures. Others showed robust proliferation despite the absence of identifiable genetic drivers, suggesting the involvement of variants in other genes or non-genetic mechanisms such as epigenetic priming or adaptive NK-cell differentiation. These results highlight the influence of both culture conditions and donor-intrinsic factors on NK-cell expansion outcomes. Integrating phenotypic and genetic analyses may improve the reproducibility and personalization of NK-cell-based manufacturing protocols for therapeutic use
Interview with Cornelius Holtorf : [Entrevista com Cornelius Holtorf]
No full textA presente entrevista, aqui apresentada em versão bilingue, tem como objetivo principal dar a conhecer, principalmente para os jovens pesquisadores, o trabalho de Cornelius Holtorf, arqueólogo importante no cenário internacional, e também algumas perspectivas inovadoras de estudos patrimoniais com que o autor vem trabalhando ao longo de sua carreira. Esperamos que a entrevista sirva para incentivar novas pesquisas em contexto brasileiro e latino-americano e para gerar reflexões importantes sobre o futuro do patrimônio nacional.The present interview, presented here in a bilingual version, has as main objective to make known, mainly for young researchers, the work of Cornelius Holtorf, an important archaeologist in the international scenario, and also some innovative perspectives of heritage studies with which the author has been working throughout their career. We hope that the interview will serve to encourage new research in the Brazilian and Latin American context and to generate important reflections on the future of national heritage.</p
Two Coons in a Wreck
No full textCover of the script for a play from the Denison's Blackface Plays series, entitles 'Two Coons in a Wreck.' This item was found in the Cornelius Recreation Club Collection. The Club put on several minstrel shows in the 1940s.This is one item from a collection of materials that once belonged to the Cornelius Recreation Club, which was active in Cornelius, Oregon from 1947-1950. Their stated purpose was "to provide recreational activities for both the young and older people of the community," and their regular social activities included dances, sports, picnics and holiday parties. The Club also hosted several minstrel shows. These offensive musical plays featured white community members wearing blackface and dressed in clownish outfits, denigrating African American people and perpetuating racist stereotypes. These shows were presumably given to all-white local audiences
The Mealtime Minstrels
No full textCover of a script for a minstrel show entitled 'The Mealtime Minstrels.' This item was found in the Cornelius Recreation Club Collection. The Club put on several minstrel shows in the 1940s.This is one item from a collection of materials that once belonged to the Cornelius Recreation Club, which was active in Cornelius, Oregon from 1947-1950. Their stated purpose was "to provide recreational activities for both the young and older people of the community," and their regular social activities included dances, sports, picnics and holiday parties. The Club also hosted several minstrel shows. These offensive musical plays featured white community members wearing blackface and dressed in clownish outfits, denigrating African American people and perpetuating racist stereotypes. These shows were presumably given to all-white local audiences
Caught with the Goods
No full textCover of the script for a play entitled 'Caught with the Goods.' In the play, a man and his valet get into a bit of a bind. This item was found in the Cornelius Recreation Club Collection.This is one item from a collection of materials that once belonged to the Cornelius Recreation Club, which was active in Cornelius, Oregon from 1947-1950. Their stated purpose was "to provide recreational activities for both the young and older people of the community," and their regular social activities included dances, sports, picnics and holiday parties. The Club also hosted several minstrel shows. These offensive musical plays featured white community members wearing blackface and dressed in clownish outfits, denigrating African American people and perpetuating racist stereotypes. These shows were presumably given to all-white local audiences
Swanee River Minstrels
No full textCover of a script for a minstrel show entitled 'Swanee River Minstrels.' This item was found in the Cornelius Recreation Club Collection. The Club put on several minstrel shows in the 1940s.This is one item from a collection of materials that once belonged to the Cornelius Recreation Club, which was active in Cornelius, Oregon from 1947-1950. Their stated purpose was "to provide recreational activities for both the young and older people of the community," and their regular social activities included dances, sports, picnics and holiday parties. The Club also hosted several minstrel shows. These offensive musical plays featured white community members wearing blackface and dressed in clownish outfits, denigrating African American people and perpetuating racist stereotypes. These shows were presumably given to all-white local audiences
Dixie Moon Minstrels
No full textCover of a script for a minstrel show entitled 'Dixie Moon Minstrels.' This item was found in the Cornelius Recreation Club Collection. The Club put on several minstrel shows in the 1940s.This is one item from a collection of materials that once belonged to the Cornelius Recreation Club, which was active in Cornelius, Oregon from 1947-1950. Their stated purpose was "to provide recreational activities for both the young and older people of the community," and their regular social activities included dances, sports, picnics and holiday parties. The Club also hosted several minstrel shows. These offensive musical plays featured white community members wearing blackface and dressed in clownish outfits, denigrating African American people and perpetuating racist stereotypes. These shows were presumably given to all-white local audiences
Mush and Poke, Butchers
No full textCover of the script for a play from the Denison's Blackface Plays series, entitles 'Mush and Poke, Butchers.' This item was found in the Cornelius Recreation Club Collection. The Club put on several minstrel shows in the 1940s.This is one item from a collection of materials that once belonged to the Cornelius Recreation Club, which was active in Cornelius, Oregon from 1947-1950. Their stated purpose was "to provide recreational activities for both the young and older people of the community," and their regular social activities included dances, sports, picnics and holiday parties. The Club also hosted several minstrel shows. These offensive musical plays featured white community members wearing blackface and dressed in clownish outfits, denigrating African American people and perpetuating racist stereotypes. These shows were presumably given to all-white local audiences
Bend Down, Sister
No full textCover of a script for a play, in which several women discuss their personal lives.This is one item from a collection of materials that once belonged to the Cornelius Recreation Club, which was active in Cornelius, Oregon from 1947-1950. Their stated purpose was "to provide recreational activities for both the young and older people of the community," and their regular social activities included dances, sports, picnics and holiday parties. The Club also hosted several minstrel shows. These offensive musical plays featured white community members wearing blackface and dressed in clownish outfits, denigrating African American people and perpetuating racist stereotypes. These shows were presumably given to all-white local audiences
Three Sermons Preached at the Two Hundred and Fiftieth Anniversary of the Founding of the Reformed Church of Bergen (organized 1660) in Jersey City, N.J.
No full textThree sermons, preached Oct. 16th and 23d, 1910, relate history of the Reformation, founding and growth of the Bergen Church, and prospects for the future. With photo of Rev. Dr. Cornelius Brett, pastor, and images of three church buildings.The Reformed Church in Europe and America; An Historical Sermon Celebrating the Two Hundred and Fiftieth Anniversary of the Founding of the Reformed Church of Bergen; A Look ForwardCover signed by Rev. Cornelius BrettOn cover: 1660, 1910, "Looking Forward", "Looking Backward", Anniversary Sermons of the Reformed Church of Bergen in New Jersey"Pastors Rev. Cornelius Brett, D.D., and Rev. John J. Moment, October sixteenth and twenty-rhird, 1910, Jersey City, N.J.
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