1,722,411 research outputs found
Kim Yoon Hwan
No full text학위논문(석사)--아주대학교 일반대학원 :응용생명공학과정,2010. 2감사의 글
Abstract
List of Figure
List of Table
Ⅰ. Introduction
Ⅱ. Theoretical Approach
A. Dextran
B. Pectin
C. Hyaluronic acid
D. Chitosan
E. Example regarding prevention of protein adsorption
F. Electrospinning
G. BCA(Bicinchoninic acid) assay
Ⅲ. Material and methods
A. Material
B. Attachment of polysaccharide on film
C. Adsorption of protein on surface
D. Analysis
E. Preparation of PVA membrane
F. Measurement of concentration amounts and adsorption amounts
Ⅳ. Results and discussions
A. Fluorescence Intensity for BSA quantitative analysis
B. Data analysis using Isoelectric point
C. Data analysis using structure of hydrogel
D. Hystogram regarding protein adsorption distribution
E. Preparation of PVA membrane
F. Polysaccharide coating on membrane surface
G. Relation between the concentration of solution and layer thickness
H. Measurement of water flow rate
I. Measurement of concentration amount and adsorption amount
J. Preparation of polysaccharide layer introduced hydrophilic branch
1 Carboxyl-terminated PEG synthesis
2 Surface modification of complex polysaccharide layer
3. Estimation of adsorption ability for modified polysaccharide layer
Ⅴ. Conclusion
Reference
국문요약MasterThis study makes to reduce a protein adsorption to the surface and obtain a high-yield product while we carry out the separation and extraction of protein. Adsorption of Protein, the most representative material among biomolecules, occurs at surface of membrane and disturbs efficient separation and extraction. To prevent the adsorption of protein, surface of membrane was modified into hydrophilic surface. In order to obtain hydrophilic surface, we used hydrophilic polysaccharide such as Pectin, Dextran, Hyaluronic acid and chitosan which is known a effective biomolecule's adsorption preventer. In order to enhance transmittance, we make membrane having a porous structure by using electrospinning. The modified surface of membrane was observed by SEM(Scanning Electron Microscope) and AFM(Atomic Force Microscope) and concentration amounts and adsorption amounts of protein were measured by BCA(Bicinchoninic acid) assay. And ultrafiltration system was used to investigate water flow rate. And the hydrophilic group is introduced into polysaccharide layer. Through this study, the protein adsorption of surface coated by polysaccharide was affected by electrostatic property between protein and polysaccharide. And the higher hydrophilicity of surface coated by polysaccharide is, the lower adsorption ratio of protein is. Also the membrane introducing MPEG-COOH on chitosan layer have an excellent performance regarding prevention of protein adsorption. These results lead us to the conclusion that membrane of several types were prepared by introducing functional group
Taxonomy of the genus Phymatura J. Sahlberg (Coleoptera: Staphylinidae: Aleocharinae) in the Korean Peninsula
No full textKim, Yoon-Ho, Ahn, Kee-Jeong (2016): Taxonomy of the genus Phymatura J. Sahlberg (Coleoptera: Staphylinidae: Aleocharinae) in the Korean Peninsula. Zootaxa 4061 (2): 189-196, DOI: 10.11646/zootaxa.4061.2.
FIGURES 12–14. Phymatura brevicollis. 12 in Taxonomy of the genus Phymatura J. Sahlberg (Coleoptera: Staphylinidae: Aleocharinae) in the Korean Peninsula
No full textFIGURES 12–14. Phymatura brevicollis. 12, male tergite VIII, dorsal aspect; 13, median lobe, lateral aspect; 14, paramere, lateral aspect. Scale bars: 0.1 mm.Published as part of Kim, Yoon-Ho & Ahn, Kee-Jeong, 2016, Taxonomy of the genus Phymatura J. Sahlberg (Coleoptera: Staphylinidae: Aleocharinae) in the Korean Peninsula, pp. 189-196 in Zootaxa 4061 (2) on page 193, DOI: 10.11646/zootaxa.4061.2.9, http://zenodo.org/record/25510
Spatial and spectral properties of entangled photons from spontaneous parametric down-conversion with a focused pump
No full textThe spatial and spectral properties of entangled photons from spontaneous parametric down-conversion (SPDC) with a focused pump are investigated with theoretical analysis and numerical simulation. Here, the spatial and spectral properties of down-converted photons are fully calculated without using the transverse momentum conservation assumption. We have obtained the spatial and spectral properties of SPDC photons under focused pumping in various SPDC configurations, including type-I, type-II for both positive and negative uniaxial crystals. Our result not only helps to understand the effect of pump focusing in SPDC better but also can find use in application of SPDC in realizing quantum information protocols. (C) 2015 Elsevier B.V. All rights reserved.1122Nsciescopu
Phymatura crenulicauda Bernhauer 1907
No full textPhymatura crenulicauda (Bernhauer, 1907) Remark. This species was reported from North Korea by Paśnik (2001). However, Paśnik misidentified the specimens as revealed by our comparison between the Korean specimens and a syntype of Atheta crenulicauda (deposited in the Field Museum, Chicago). We could not identify the Korean specimens with confidence at this stage. They need a more detailed study to be correctly identified.Published as part of Kim, Yoon-Ho & Ahn, Kee-Jeong, 2016, Taxonomy of the genus Phymatura J. Sahlberg (Coleoptera: Staphylinidae: Aleocharinae) in the Korean Peninsula, pp. 189-196 in Zootaxa 4061 (2) on page 195, DOI: 10.11646/zootaxa.4061.2.9, http://zenodo.org/record/25510
Continuous heat shock enhances translational initiation directed by internal ribosomal entry site
No full textMany cellular mRNAs contain internal ribosomal entry sites (IRES) that become functional under conditions of cellular stress, when the rate of protein synthesis for most cellular mRNA is reduced. Internal ribosomal entry increases in response to hypoxia, cell differentiation, apoptosis, gamma irradiation, and heat shock. Heat shock is the principal cellular stress in which general cap-dependent translation is inhibited. On the other hand, heat shock induces the preferential translation of a small class of mRNA, called heat shock protein (HSP) mRNAs, which probably occurs because little or no eIF4F activity is required for their translation. In this study, we found that continuous heat stress enhances expression of the heat shock protein BiP at the level of translation. Interestingly, heat stress also enhanced the viral IRES-dependent translation of encephalomyocarditis virus and hepatitis C virus but not poliovirus. Although several BiP inducers increased BiP protein expression, BiP IRES-dependent translation was enhanced only during heat shock, suggesting that heat shock is a specific inducer for BiP IRES-dependent translation. Taken together, these results indicate that the mechanism of IRES-dependent translation can be used during heat shock and suggest that this translational mechanism may be critical to the survival and proliferation of cells under stress. (C) 2002 Elsevier Science (USA). All rights reserved.
AU-rich element-mediated mRNA decay via the butyrate response factor 1 controls cellular levels of polyadenylated replication-dependent histone mRNAs
No full textReplication-dependent histone (RDH) mRNAs have a nonpolyadenylated 3-UTR that ends in a highly conserved stem-loop structure. Nonetheless, a subset of RDH mRNAs has a poly(A) tail under physiological conditions. The biological meaning of poly(A)-containing (+) RDH mRNAs and details of their biosynthesis remain elusive. Here, using HeLa cells and Western blotting, qRT-PCR, and biotinylated RNA pulldown assays, we show that poly(A)(+) RDH mRNAs are post-transcriptionally regulated via adenylate- and uridylate-rich element-mediated mRNA decay (AMD). We observed that the rapid degradation of poly(A)(+) RDH mRNA is driven by butyrate response factor 1 (BRF1; also known as ZFP36 ring finger protein-like 1) under normal conditions. Conversely, cellular stresses such as UV C irradiation promoted BRF1 degradation, increased the association of Hu antigen R (HuR; also known as ELAV-like RNA-binding protein 1) with the 3-UTR of poly(A)(+) RDH mRNAs, and eventually stabilized the poly(A)(+) RDH mRNAs. Collectively, our results provide evidence that AMD surveils poly(A)(+) RDH mRNAs via BRF1-mediated degradation under physiological conditions.
La protein is required for efficient translation driven by encephalomyocarditis virus internal ribosomal entry site
No full textTranslation of internal ribosomal entry site (IRES)-dependent mRNAs is mediated by RNA-binding proteins as well as canonical translation factors. In order to elucidate the roles of RNA-binding proteins in IRES-dependent translation, the role of polypyrimidine tract-binding protein (PTB) and La protein in encephalomyocarditis virus (EMCV) IRES-dependent translation was investigated. PTB was required for efficient EMCV IRES-driven translation but, intriguingly, an excess of PTB suppressed it. Such a translational suppression by surplus PTB was relieved by addition of La protein. A possible role for La protein in IRES-dependent translation is discussed.
Experimental characterization of quantum polarization of three-photon states
No full textWe experimentally investigate various quantum polarization features of three-photon quantum states, including product and entangled states with varying purity. The three-photon quantum states are categorized into six classes based on the rotation symmetry of mean, variance, and skewness of the polarization distribution. The representative three-photon quantum states in each category are prepared from double-pair emission from pulsed spontaneous parametric down-conversion and quantum interferometry. We demonstrate that the three-photon quantum states show interesting quantum polarization properties, such as maximum sum-uncertainty and hidden polarizations.111Nsciescopu
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