1,720,971 research outputs found
Crowding and confinement effects on enzyme stability in mesoporous silicas
No full textTo understand the protein functions within a cell, where proteins exist in an extremely crowded and confined state, various modeling and experimental methods have been proposed. Here, we propose a new experimental approach to modulate the macromolecular crowding and/or confinement effects by using mesoporous silicas with two different pore structures. SBA-15 and MSU-F with linear and mesocellular pore structures, respectively, were used to adsorb a model enzyme, glucose oxidase (GOx), in various concentrations ranging from 3 to 430 mg ml(-1). The concentration of adsorbed GOx in the mesopores, representing the degree of crowding, showed a good correlation with thermal enzyme stability. Interestingly, the increase of thermal stability as a function of macromolecular crowding showed different correlations depending on the pore structure of mesoporous silicas. It represents that combination of crowding and confinement effects can promote different microenvironments for enzyme molecules, while mesoporous silicas can impose controlled crowding and confinement effects on enzymes due to their uniform and tunable pore structures. It is anticipated that this new and simple approach can provide a tool to elucidate crowding and confinement effects on the protein functions, including its stability in vivo, because the mesopore environments could mimic the real macromolecular cell system under crowding.
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
(The) effects of home environment modification on occupational performance and fall efficacy in rural community dwelling elderly
No full text재활학과/석사본 연구는 가정환경 수정이 농촌 노인들의 작업 수행도 및 낙상 효능감에 미치는 영향에 대해 알아보고자 한다. 연구 대상자는 65세 이상의 노인들로 농사 경험이 최소 5년 이상으로 지속적인 농사로 인해 요통, 관절염과 같이 근골격계 질환을 가지고 있지만 이외 다른 의학적 진단을 받지 않은 15명으로 구성하였으며 연구 기간은 2010년 5월부터 2011년 6월까지 약 한 달간 실시하였다. 연구기간 동안 연구자가 직접 대상 가구에 방문 하여 환경적 문제점과 대상자들이 요구사항에 대해 면담을 실시하였다. 한국 주거학회에서 제시한 노인 가구 주택개조 지침서를 기준으로 농촌 가정환경 실정에 맞는 가정환경 수정 체크리스트를 구성하였으며 현관, 거실, 침실, 주방, 욕실 및 화장실을 가정환경 수정 체크리스트를 사용하여 가정 내 환경적 장애물에 대해 파악하고 작업 수행능력 측정 평가 도구인 Canadian Occupational Performance Measure (COPM)를 본 연구에 맞게 수정하여 가정 내에서 수행하기 어려운 활동이거나 일상생활을 수행 하고 있는 장소가 얼마나 중요한지에 대해 평가하였으며 한국형 낙상효능감 척도(K-FES)를 사용하여 대상자들의 낙상효능감에 대해 알아보았다. 가정환경 수정 전 조사된 결과를 토대로 시공업체와 상의 하에 가정환경 수정을 실시하였으며 15가구에 총 122개의 물품을 제공 하였다. 수정 후 COPM을 사용하여 변화된 환경과 수행 활동에 대한 대상자들의 만족도를 재평가하였다. 그 결과 가정환경 수정 전 장소에 따른 문제점은 욕실, 화장실, 현관, 주방, 거실 및 침실 순으로 나타났지만 장소에 따른 수행도의 중요도는 주방에서의 활동이 가장 높게 나타났으며 환경적 문제점이 가장 많았던 욕실의 중요도는 상대적으로 가장 낮게 나타났다. 수행 활동의 문제점과 중요도를 토대로 가정환경 수정을 실시한 결과 전체 대상자들의 작업 수행도와 장소에 따른 작업 수행도 및 낙상효능감은 통계학적으로 유의한 차이가 나타났으며 가정환경 수정 전 중요도가 가장 높았던 주방은 만족도 또한 가장 높았으며 거실, 화장실, 침실, 욕실, 현관 순으로 나타났다. 본 연구에서는 가정환경 수정을 통해 농촌 노인들이 살고 있는 가정에서 환경적 장애물로 나타나는 요소들을 제거 및 수정하여 노인들의 작업 수행력과 낙상효능감을 향상하고자 하였으며 이러한 결과는 노인들이 가정 내에서 독립적인 일상생활활동 수행이 가능하도록 긍정적인 영향을 줄 수 있음을 시사한다.ope
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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