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Transcription: Retelling of Hamale Khatun
Transcription and free English translation of Naseema Ali reciting "Hamale Khatun," in the Nagar dialect. In this popular story Hamale Khatun is invited to the house of a paranormal Queen, and stitches clothing for all of her sons and daughters at their weddings. When she returns home, Hamale Khatun comes with warnings to remain moral by the Queen, and tries to make sure her children obey them
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Interlinear-glossed text: Retelling of Hamale Khatun by Naseema Ali
Transcription, translation and linguistic (morphemic) analysis of the story "Hamale Khatun" narrated by Naseema Ali, a speaker of the Nagar dialect of Burushaski. In this popular story Hamale Khatun is invited to the house of a paranormal Queen, and stitches clothing for all of her sons and daughters at their weddings. When she returns home, Hamale Khatun comes with warnings to remain moral by the Queen, and tries to make sure her children obey them
KHATUN MST RESHMA
학위논문(석사)--아주대학교 일반대학원 :의생명과학과,2019. 2There are evidences TIS21/BTG2/PC3, an antiproliferative gene, is essential for multiple biological functions such as cell proliferation, growth, differentiation, senescence and cell death. However, no study has been found that examined its role in muscle function and maintenance. In this study, we investigated whether TIS21 has any role in the regulation of muscle protein, myosin heavy chain (MyHCs), expression in gastrocnemius (GA) skeletal muscle of male and female mice along with their ageing process. Firstly, we measured specific changes of GA muscle and body weight and their ratio at different age of C57BL/6J male and female mice. The absolute muscle and body weight were significantly higher in the TIS21-knock out (KO) young (12 wk) and mid-old (38 wk) male mice as compared with TIS21-wild type (WT). However, there was no significant change in the muscle and body weight and their ratio between the TIS21 WT and TIS21 KO female mice. Secondly, our immunoblot data revealed that KO of TIS21 gene results significant reduction of MyHCs protein expression in GA of mid-old (34 wk-38 wk) female mice. The reduction was also significant in the old female mice (19 months). However, TIS21 KO male mice did not show that kind of significant decline in MyHCs protein expression compared to its WT in ageing response. Furthermore, analysis of mRNA expression by Real-time PCR showed that the transcription of MyHC2a and MyHC2b (type II fiber isoforms) significantly downregulated in the old TIS21 KO-female mice (19 months) rather than old male mice (24 months) compared to its WT. Finally, transient transfection including TIS21 overexpression into C2C12 cells following luciferase assay demonstrates that TIS21 might regulate the expression of MyHCs from their promoter level. Overall, our data implicate a potential role of TIS21 in MyHCs expression, where TIS21 controls the transcriptional level in female mice.
Keywords: Skeletal Muscle, Myosin heavy chain, TIS21 and Ageing.I. INTRODUCTION 1
II. MATERIALS AND METHODS 5
2.1. Ethical statement 5
2.2. Animals and tissue sample collection 5
2.3. Cell culture. 5
2.4. Genomic DNA isolation and genotyping of TIS21 WT & TIS21 KO mice 6
2.5. Muscle homogenization for western blotting. 7
2.6. Western blotting analysis. 7
2.7. RNA isolation and quality control. 8
2.8. cDNA synthesis and Quantitative Real -Time PCR analysis. 9
2.9. Luciferase reporter vector construct preparation. 9
2.10. Transfection and Luciferase assay 10
2.11. Statistical analysis. 11
III. RESULTS 14
3.1. Genotyping of TIS21 Wild type (TIS21 WT) and TIS21 Knockout (TIS21 KO) mice 14
3.2. Changes in body weight, GA muscle mass and GA muscle mass vs body weight ratio in TIS21 WT and TIS21 KO male and female mice 17
3.3. Effect of TIS21 in myosin heavy chain (MyHCs) protein expression in gastrocnemius skeletal muscle of male and female mice. 22
3.4. Integrity checking of total RNA isolated from gastrocnemius muscle of TIS21 WT and TIS21 KO male and female mice. 31
3.5. Effect of TIS21 in mRNA expression of myosin heavy chain (MyHC) isoforms in gastrocnemius skeletal muscle of male and female mice 34
3.6. Molecular function of TIS21 in the regulation of MyHCs expression 37
IV. DISCUSSION 40
REFERENCES. 43
-국문요약- 50Maste
Damascus (Syria): Murshidiyya Madrasa and Tomb (Khadija Khatun): plan
Box 22, Folder 01. Ink and pencil drawings. Damascus (Syria): Murshidiyya Tomb (al-Turba al-Murshidiyya, Khadija Khatun Tomb, Turba of Khadija Khatun). Drawings depict a ground plan (below) and a detail of a bracket over the entrance portal (top). These versions of the drawings not published. Title and identification based on drawing published in Ernst Herzfeld, "Damascus: Studies in Architecture – III," Ars Islamica vols. 11-12 (1946), figure 87 and 109. Published caption for fig. 87 reads: "Damascus, Salihiya, al-Murshidiya (Khadidja Khatun), Bracket." Published caption for fig. 109 reads: "Khadidja Khatun." German identification notes written on front in gray pencil include: "Dam. Salihiyya; Khadidja Khatun 634.
Baghdad (Iraq): Zumurrud Khatun Tomb (Sitta Zubayda): muqarnas dome
Box 21, Folder 07. Ink drawing. Baghdad (Iraq): Zumurrud Khatun Tomb (Turbat Zumurrud Khatun, Mausoleum of Zumurrud Khatun, Sitta Zubayda Tomb). Drawing depicts a study of the muqarnas dome. This drawing published in Ernst Herzfeld, "Damascus: Studies in Architecture – I," Ars Islamica 9 (1942), figure 18. Published caption for fig. 18 reads: "Baghdad, Sitt Zubaida, Vault." Title and identificaton based on this publication
Authorship Attribution Dataset in Bangla 2
A dataset consisting of text samples from 6 authors with 350 samples per author and total word count of 2.3+ million
Authorship Attribution Dataset in Bangla 2
A dataset consisting of text samples from 6 authors with 350 samples per author and total word count of 2.3+ million
BAAD6: Bangla Authorship Attribution Dataset
A dataset consisting of text samples from 6 authors with 350 samples per author and total word count of 2.3+ million
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Conversation about annual grass cutting
Khatun Bibi and Sartaj Bibi discuss the daily routines of household chores during the grass cutting season. They also talk about the hardships they face during this particular season. Khatun Bibi is a 51-year old housewife. Sartaj Bibi is a 23 year old housewife
Baghdad (Iraq): Zumurrud Khatun Tomb (Sitta Zubayda): muqarnas dome
Box 21, Folder 07. Pencil drawing. Baghdad (Iraq): Zumurrud Khatun Tomb (Turbat Zumurrud Khatun, Mausoleum of Zumurrud Khatun, Sitta Zubayda Tomb). Drawing depicts a study of the muqarnas dome. This version of the drawing is not published. Title and identification based on drawing published in Ernst Herzfeld, "Damascus: Studies in Architecture – I," Ars Islamica 9 (1942), figure 18. Published caption for fig. 18 reads: "Baghdad, Sitt Zubaida, Vault.
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