1,721,021 research outputs found
Data from "Dual-specificity phosphatase 5 (DUSP5) controls the localized inhibition, propagation and transforming potential of ERK signaling"
No full textThis repository contains the data used to construct figures in the paper "Dual-specificity phosphatase 5 (DUSP5) controls the localized inhibition, propagation and transforming potential of ERK signaling" by Kidger et al., published in Proceedings of the National Academy of Sciences, USA. All numerical data are included in a Microsoft Excel file labelled "numerical data for Kidger et al 2017". Tabs within this spreadsheet are labelled with the figures names the data correspond to in the publication. Similarly, images and Western blot scans are all labelled with figure titles. Please see the publication for details of experimental procedures.Details of experimental procedures can be found in the Kidger et al. 2017 publication. Detailed high content microscopy methodology can also be found in the following paper:
Caunt, Kidger and Keyse, Methods Mol Biol. 2016;1447:197-215. doi: 10.1007/978-1-4939-3746-2_12.
Data provided in the Microsoft Excel file include a combination of data derived from high content microscopy and automated analysis (GE Healthcare IN Cell 2000 microscope and IN Cell Developer software), immunoblot analysis from scanned images using a Licor Odyssey scanner, quantitative PCR techniques and a small number of manual counting assays for cellular senescence and focus formation.For high content microscopy, single cell data is shown as raw arbitrary fluorescence unit (AFU) values. For population-averaged data, where immunostaining experiments were pooled from several independent experiments, each of which usually had several replicate samples per plate, average AFU values from entire wells were normalised as percentages of values from control wells in each plate and applied across all technical replicate wells in the 96-well plate, prior to data pooling between independent experiments. Other types of data derived from high content microscopy are represented as discrete values. For example, an AFU cutoff for label intensity above background values was used to assign whether a cell had entered S-phase within a given pulse window of fluorescent labelling. All analysis of nuclear and cytoplasmic intensity values was carried out using a custom routine described in Caunt, Kidger and Keyse, Methods Mol Biol. 2016;1447:197-215. doi: 10.1007/978-1-4939-3746-2_12.
Where possible, we have included Western blot as original blot scans, as well as raw intensity values in the spreadsheet derived from the Licor scanner, and also values corrected for loading and normalised to control samples contained in each replicate blot (or normalised according to the mean signal intensity across the blot).Please see Kidger et al 2017 for details of experimental procedures and equipment used
Analysis of multisite phosphorylation of the p53 tumor-suppressor protein by tryptic phosphopeptide mapping
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Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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