1,721,193 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Study on the Role of Slit1 Protein Associated with Angiogenesis of Prostate Cancer Metastasis
No full text攝護腺癌(Prostate cancer)為男性常見的癌症之一，根據行政院衛生署統計資料，攝護腺癌死亡率為癌症死亡率第七位。攝護腺癌的生長和分化受到雄性荷爾蒙調控，在癌症進展中，患者通常在治療過程中服用荷爾蒙拮抗藥物，而使攝護腺癌由荷爾蒙依賴型轉變為非荷爾蒙依賴型，進而造成癌症的惡化。本實驗室以荷爾蒙拮抗藥物bicalutamide (Casodex R )建立具有抗藥性之攝護腺癌細胞株，模擬臨床上攝護腺癌患者在接受荷爾蒙治療法失敗的情況。隨後以微陣列技術分析具有抗藥性細胞株與其對照組細胞株之間基因表現量之差異，發現Slit1 基因可能參與調控攝護腺癌之發展與惡化。進一步利用組織微陣列晶片觀察Slit1 蛋白的表現量在不同階段的攝護腺癌中是否有所不同，結果發現Slit1 主要表現於轉移型之攝護腺癌患者。
Slit 蛋白家族為分泌型蛋白，與其受體Roundabout (Robo)受體相接後，主要調控神經發育過程中軸突引導(axon guildance)以及神經遷移(neuronal migration)作用。研究指出與發育過程相關之蛋白往往在癌症發展中扮演重要的角色，Slit2 蛋白已被報導在不同腫瘤中皆有表現，但其表現量與癌症進展及存活率並無一致性。此外，過去文獻指出Slit2 蛋白具有促進血管新生之作用，誘導血管內皮細胞形成管狀構造並增加其遷移能力，使癌細胞透過新生血管獲得足夠養分以利生長。Slit3 也被發現透過誘導內皮細胞增生、遷移以及血管構造之形成來促進血管新生現象產生。然而尚未有文獻探討Slit1 蛋白在血管新生所扮演之角色為何，因此本研究利用in vitro 以及in vivo 方式探討Slit1 是否會造成血管新生現象。首先，利用quantitative Real-time PCR 分析人類臍靜脈內皮細胞中Slit 家族以及其受體Robo 家族基因表現情形，結果發現人類臍靜脈內皮細胞並不表現Slit1，而受體基因表現量最高為Robo4，Robo1 則次之，並利用免疫螢光染色法證實Robo1以及Robo4 表現情形。接著以酵素免疫吸附試驗(ELISA)觀察Slit1 以及Robo1、Robo4 結合情形，結果顯示Slit1 蛋白與Robo1 受體結合效力較Robo4 高出許多，推測Slit1 蛋白會與Robo1 受體結合進入細胞中。以Slit1 蛋白以及條件培養液處理人類臍靜脈內皮細胞後，觀察細胞增生(cell proliferation)、管狀構造形成(tube formation)以及傷口癒合檢測(wound healing)。結果顯示Slit1 蛋白僅有顯著性增加管狀構造之形成且具有一劑量效應關係，然對HUVEC 細胞之生長與細胞爬行能力並無顯著性差異。將Robo4 胜肽(peptide)與Slit1 蛋白預先混合後再給予HUVEC 細胞，其管狀構造形成現象也隨之消失，證實Slit 蛋白具有促進血管新生之功能。而在in vivo 部分，利用雞胚絨毛尿囊膜試驗觀察Slit1 是否會造成血管新生之現象，結果也發現Slit1 確實會誘導血管新生現象產生。未來將進一步針對管狀構造形成探討Slit1 造成血管新生之機制。口試委員會審定書
謝誌
縮寫表 (Abbreviations) ................................................................................ IV
圖表目錄 (Figures and Tables) ...................................................................... V
中文摘要.......................................................................................... VI
英文摘要....................................................................................... VIII
第一章 緒論 (Introduction)
1.1 攝護腺癌之背景介紹............................................................................... 1
1.2 攝護腺癌與血管新生之關連性............................................................... 2
1.3 血管內皮細胞與血管新生....................................................................... 3
1.4 攝護腺癌與Slit 之關係 ........................................................................... 5
1.5 Slit 之背景介紹 ........................................................................... 5
1.6 Slit 蛋白與癌症的相關性 ......................................................................... 6
1.7 Slit 蛋白與血管新生之相關性 ................................................................. 8
1.8 Slit 蛋白之受體Robo 與血管新生的關連性 .......................................... 9
1.9 研究動機................................................................................... 11
第二章 實驗材料與方法 (Materials and Methods)
2.1 實驗材料................................................................................... 12
2.2.1 人類臍靜脈內皮細胞 (Human Umbilical Vein Endothelial Cells, HUVECs)之
分離與培養...................................................................................... 17
2.2.2 免疫組織染色 (Immunohistochemical staining) ............................................. 17
2.2.3 免疫螢光染色 (Immunoflourecence) ................................................. 18
2.2.4 定量即時聚合酶連鎖反應 (Quantitative Real Time Polymerase Chain
Reaction, qRT-PCR) ....................................................................... 19
2.2.5 酵素免疫吸附試驗 (Enzyme-linked immunosorbent assay, ELISA).............. 19
2.2.6 慢病毒製備與感染 (Lentivirus production and infection) .............................. 20
2.2.7 西方墨點法 (Western blot analysis) ................................................... 21
2.2.8 細胞增生試驗 (Cell proliferation assay) ............................................ 22
2.2.9 傷口癒合檢測 (Wound healing assay) ............................................... 23
2.2.10 管狀構造形成試驗 (Tube formation assay) .................................... 23
2.2.11 雞胚絨毛尿囊膜試驗 (Chick Chorioallantoic Membrane assay) .................. 24
2.2.12 統計分析(Statistic analysis) .............................................................. 24
第三章 實驗結果 (Results)
3.1 Slit1 蛋白表現量在攝護腺癌進展過程中的變化 ............................................... 26
3.2 內皮細胞之細胞特異性標誌CD31 以及CD105 存在於人類臍靜脈內皮細胞的
細胞膜表面...................................................................................... 26
3.3 Slit 家族以及其受體Robo 家族於人類臍靜脈內皮細胞基因的表現量 .......... 27
3.4 Slit1 之受體Robo1 以及Robo4 表現於人類臍靜脈內皮細胞之細胞膜表面 .. 27
3.5 Slit1 與其受體Robo1、Robo4 結合效力的比較 .................................. 28
3.6 含Slit1 之pWPI 載體感染293T 細胞 .................................................. 29
3.7 Slit1 對人類臍靜脈內皮細胞增生的影響 ............................................. 29
3.8 Slit1 蛋白影響人類臍靜脈內皮細胞遷移的能力 ............................................... 30
3.9 Slit1 蛋白誘導人類臍靜脈內皮細胞管狀構造形成的作用 ............................... 31
3.10 Slit1 蛋白於體內試驗中誘導血管新生作用 ....................................... 33
第四章 討論 (Discussion)
4.1 Slit1 蛋白造成血管新生現象 ................................................................. 35
4.2 Slit1 蛋白以及其受體Robo 於神經發育過程之角色與癌症之相關性 ............ 36
4.3 神經軸突導向分子與癌症進展過程之相關性..................................... 39
4.4 促血管新生因子所造成血管新生現象之探討..................................... 40
4.4 Slit1 於轉移型攝護腺癌表現量之探討 ................................................. 41
第五章 總結 (Conclusion) ......................................................................... 43
參考文獻 (References) ............................................................................... 44
圖表集 (Figures and Tables) ....................................................................... 5
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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