17 research outputs found

    The effects of putrescine pre-treatment on osmotic stress responses in drought-tolerant and drought-sensitive wheat seedlings

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    Recent studies have demonstrated that exogenous polyamines have protective effects under various stress condition. A broader understanding of the role of the polyamine pool fine regulation and the alterations of polyamine-related physiological processes could be obtained by comparing the stress effects in different genotypes. In this study, the impact of pretreatment with putrescine in response to osmotic stress was investigated in the droughttolerant Katya and drought-sensitive Zora wheat (Triticum aestivum) cultivars. Photosynthetic performance, in vivo thermoluminescence emission from leaves, leaf temperature, polyamine and salicylic acid levels, contents of osmoprotectants, and activities of antioxidant enzymes in the leaves were investigated not only to reveal differences in the physiological processes associated to drought tolerance, but to highlight the modulating strategies of polyamine metabolism between a drought-tolerant and a drought-sensitive wheat genotype. Results showed that the tolerance of Katya under osmotic stress conditions was characterized by higher photosynthetic ability, stable charge separation across the thylakoid membrane in photosystem II, higher proline accumulation and antioxidant activity. Thermoluminescence also revealed differences between the two varieties − a downshift of the B band and an increase of the afterglow band under osmotic stress in Zora, providing original complementary information to leaf photosynthesis. Katya variety exhibited higher constitutive levels of the signaling molecules putrescine and salicylic acid compared to the sensitive Zora. However, responses to exogenous putrescine were more advantageous for the sensitive variety under PEG treatment, which may be in relation with the decreased catabolism of polyamines, suggesting the increased need for polyamine under stress conditions

    Demodicosis Cutis and Metabolic Disturbances

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    Cutaneous demodicosis (CD) is a pilosebaceous unit disease, overlapping clinically with other facial dermatoses, mainly rosacea, and acne. It is usually improved by acaricidal monotherapy. This study investigates the association of CD with metabolic disturbances. It was conducted with 141 patients with primary and secondary CD. The study investigated the correlation between CD and dyslipidemia based on disturbances in total cholesterol (TC) and Low-Density Lipoproteins (LDL) and between CD and impaired glucose tolerance, diagnosed with elevated fasting blood sugar levels, 120 minutes blood sugar levels in oral glucose tolerance test (OGTT) and the mathematically calculated Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index. The study results were verified with control groups. It was established that patients with CD do not show statistically significant deviations in serum lipoproteins compared with the control group. However, the patients with CD tended to have impaired glucose tolerance, demonstrated with elevated fasting glucose levels and elevated HOMA-IR, compared to the control group. Interestingly enough, the tendency towards prediabetes and diabetes was not confirmed by the 120 minutes blood glucose levels of the OGTT. The presented data confirm the need for testing impaired glucose tolerance in every patient with cutaneous demodicosis. Based on the presented evidence, it is recommended to perform the screening by evaluating fasting glucose levels and fasting insulin levels, followed by calculating the HOMA-IR index

    Lung perfusion scintigraphy in pulmonary carcinoma and pulmonectomy

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    PURPOSE: The surgical treatment is the most successful treatment of non-small cell lung cancer (NSCLC) in oncology practice. Lung perfusion scintigraphy as a non-invasive method for evaluation of the perfusion defect related to tumor localization in lung cancer patients is considered the differentiating line of pulmonary tests to predict post-operative lung function.MATERIALS AND METHODS: We analyzed retrospectively the value of lung perfusion scintigraphy in 10 cases with primary lung carcinoma who had a pulmonectomy (9 left and 1 right pulmonectomy).RESULTS: Changes in affected lung perfusion varied from impaired in different rate to perfusion defect in at least 3 lung segments (presented in 3 of the patients), 4 lung segments (present in 2 of the patients), or parts of segments, or defect affecting whole lobe. Impaired perfusion in a zone with polycyclic outlines in homolateral hilum was present in 7 of the patients. Perfusion indices at localization in the left lung (8 of the patients) were mean = 36,79%, SD=19,35%, SE=6,8%.CONCLUSION: Lung perfusion scintigraphy is a valuable method in the complex of pre-operative examinations to define the extent of the impairments in pulmonary perfusion and predicting the post-operative pulmonary function when pulmonectomy is about to be elaborated. The rate of perfusion changes and the percentage of involvement of the affected lung in the common pulmonary function correlate with survival. The low perfusion in the affected lung is not necessarily a contraindication for operative treatment

    Pulmonary perfusion scintigraphy in lung carcinoma and lobectomy

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    PURPOSE: Most successful treatment of the lung cancer patients is the surgical resection. Lung perfusion scintigraphy is established method in the complex pre-operative diagnostics of lung carcinoma. It is used for selection of candidates for surgical treatment and in determination of operability and in decision on the operation volume.MATERIALS AND METHODS: We analyzed retrospectively the value of lung perfusion scintigraphy in 27 cases with primary lung carcinoma who had a lobectomy. Survival probability was calculated by Kaplan- Meier method. The log rank test was used to compare survival rates between groups.RESULTS: All patients with lobectomy were staged pre-operatively by noninvasive procedures at stages I-IIIA. The lung perfusion scintigraphy demonstrated an enlarged mediastinum at 1 of the patients. Impaired perfusion in homolateral hilum and in the contralateral hilum was found in some of the patients. Changes in affected lung perfusion varied from impaired in different rate to perfusion defect. Perfusion indices at affected lung have mean=48,59%. The survival of patients with Perfusion index less than 49% was mean = 56,333 months, and median = 35 months (3 years). The survival of patients with Perfusion index more than 49% was mean = 99,214 months, and median = 64 months (5.3 years). For I and II stage mean survival time was 120,727 months, and median survival time was 112 months (9.3 years). For IIIA and IIIB stage mean survival time was 48,667 months, and median survival time was 29 months (2,4 years).CONCLUSION: Lung perfusion scintigraphy is a valuable method in pre-operative diagnosis of lung carcinoma in determination of the extent of perfusion impairment in the affected lung and for determination of functional operability. Lobectomy is possible when perfusion in affected lung is over 41%, but the lower perfusion is not surely a contraindication for lobectomy. The extent of perfusion impairment is proportional to survival rate. Patients with lower perfusion impairments have more than 5 years median survival. In postoperative period perfusion scintigraphy shows re-distribution of perfusion in the healthy lung and in the rest of the operated lung. The survival of patients with lobectomy is corresponding to the clinical stage statistically significant

    Different Responses to Water Deficit of Two Common Winter Wheat Varieties: Physiological and Biochemical Characteristics

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    Since water scarcity is one of the main risks for the future of agriculture, studying the ability of different wheat genotypes to tolerate a water deficit is fundamental. This study examined the responses of two hybrid wheat varieties (Gizda and Fermer) with different drought resistance to moderate (3 days) and severe (7 days) drought stress, as well as their post-stress recovery to understand their underlying defense strategies and adaptive mechanisms in more detail. To this end, the dehydration-induced alterations in the electrolyte leakage, photosynthetic pigment content, membrane fluidity, energy interaction between pigment–protein complexes, primary photosynthetic reactions, photosynthetic and stress-induced proteins, and antioxidant responses were analyzed in order to unravel the different physiological and biochemical strategies of both wheat varieties. The results demonstrated that Gizda plants are more tolerant to severe dehydration compared to Fermer, as evidenced by the lower decrease in leaf water and pigment content, lower inhibition of photosystem II (PSII) photochemistry and dissipation of thermal energy, as well as lower dehydrins’ content. Some of defense mechanisms by which Gizda variety can tolerate drought stress involve the maintenance of decreased chlorophyll content in leaves, increased fluidity of the thylakoid membranes causing structural alterations in the photosynthetic apparatus, as well as dehydration-induced accumulation of early light-induced proteins (ELIPs), an increased capacity for PSI cyclic electron transport and enhanced antioxidant enzyme activity (SOD and APX), thus alleviating oxidative damage. Furthermore, the leaf content of total phenols, flavonoids, and lipid-soluble antioxidant metabolites was higher in Gizda than in Fermer

    Introducing the Topical Collection: 'Climate change communication and the IPCC'

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    The Intergovernmental Panel on Climate Change (IPCC) is widely regarded as the most important and authoritative source on climate change, its impacts and how to tackle the rising emissions that drive it. With this authority comes a responsibility to ensure the information is communicated effectively to policymakers, citizens and those who rely on the information for their lives and livelihoods. How the IPCC communicates the information in its reports via its official materials (e.g. Summary for Policymakers, presentations, FAQs), through different channels (e.g. interaction with journalists, social media, outreach events in different countries) and to its main audience of policymakers as well as others (including media, business, NGOs, education) has been the subject of intense analysis in the past. How different types of evidence are included in IPCC reports, particularly indigenous and local knowledge, is also a rich vein of discussion. Similarly, the representativeness of the leadership, staff and author teams in terms of gender, geographical balance and diversity of expert perspectives is key to ensuring all voices are heard and all relevant evidence is considered. The IPCC has a long history of asking the research and global communications communities for input to its evolving communications strategy. In 2016, the IPCC convened an Expert Meeting on Communication, which led to a number of recommendations to enhance IPCC communications activities, strategy and capacity (IPCC 2016; see also Lynn and Peeva, this issue). With the Sixth Assessment Report (AR6) cycle nearing completion,Footnote 1 now is an important moment to, once again, take stock of the evolving IPCC communications strategy, a time to critically reflect on successes, challenges, lessons learned and best practice for future reports. We also intend for this Topical Collection (TC) to speak to other institutions seeking to further their climate science engagement efforts at global, national, regional and local scales

    Betti numbers of Koszul algebras and codimension two matrix factorizations

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    This thesis consists of two projects on the structure of free resolutions in commutative algebra. After developing some necessary background, we prove a structure theorem in Chapter 3 for the defining ideals of Koszul almost complete intersections and, in the process, give an affirmative answer for all such rings to a question of Avramov, Conca, and Iyengar about the Betti numbers of Koszul algebras. In Chapter 4, we study the codimension two matrix factorizations of Eisenbud and Peeva. Each matrix factorization compactly encodes the data of a free resolution of its corresponding matrix factorization module. By showing that each matrix factorization also encodes a canonical system of higher homotopies on this free resolution, we are able to construct a functor from codimension two matrix factorizations to the singularity category of the corresponding complete intersection. This represents the first step towards reconciling higher codimension matrix factorizations with known generalizations of a theorem of Buchweitz and Orlov in the hypersurface case.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-08-01The student, Matthew Mastroeni, accepted the attached license on 2018-06-27 at 12:18.The student, Matthew Mastroeni, submitted this Dissertation for approval on 2018-06-27 at 12:33.This Dissertation was approved for publication on 2018-06-28 at 11:56.DSpace SAF Submission Ingestion Package generated from Vireo submission #12676 on 2018-09-27 at 11:16:09Made available in DSpace on 2018-09-27T16:30:14Z (GMT). No. of bitstreams: 2 MASTROENI-DISSERTATION-2018.pdf: 478509 bytes, checksum: 25720cb3828353404aba12968907f25d (MD5) LICENSE.txt: 4214 bytes, checksum: 589255fccd66caf76c464d80d44845bc (MD5) Previous issue date: 2018-06-28Embargo set by: Seth Robbins for item 107756 Lift date: 2020-09-27T16:30:34Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 107756 Lift date: 2020-09-27T16:31:43Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 107756 Lift date: 2020-09-27T16:34:29Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemU of I Only Restriction Lifted for Item 107756 on 2020-09-28T09:15:13Z
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