1,721,017 research outputs found
Investigation of cyclin D1 (G870A) gene polymorphisms in patients with gastric carcinoma
No full textObjectives: Gastric carcinoma is the second most common cancer in both man and woman in Turkey. Many environmental and individual etiological factors have been investigated including genetic factors. Cyclins which have an important role in cell cycle have been studied, particularly cyclin D1 gene polymorphism has been found to have a role in some cancers. In this study, association between cyclin D1 gene polymorphism and gastric carcinoma was searched in our community.Materials and Methods: Fifty-eight gastric carcinoma patients who had been admitted at Bursa Yuksek Ihtisas Hospital General Surgery Department between 2005 and 2010 and 59 healthy individuals have been included in the study. Samples have been subjected to genetic analysis by PCR-RFLP method in Medical Genetics Department laboratory at Uludag University.Results: GG genotype was found in 16 (28%), AG genotype in 28 (49%), AA genotype in 13 (% 23) in patient group. In control group, numbers was 11 (19%), 31 (54%) and 17 (29%) respectively. The difference of GG, AG and AA genotypes between patient and control groups was not statistically significant. G allele was found 60 (53%) and an allele 54 (47%) in patient group and 51 (45%), and 66 (55%) in control group.Conclusion: Our knowledge, this study is the first to evaluate the relation between gastric carcinoma and cyclin D1 polymorphism in Turkish population. Our results show that there is no any association between gastric carcinoma and cyclin D1 polymorphism in the community which is represented by our study and control groups
Does MBL2 codon 54 polymorphism play a role in the pathogenesis of psoriasis?
Get PDFYaykasli, Kursat/0000-0001-7550-6370WOS: 000328543600027PubMed: 23113841Background Psoriasis is a T cell-mediated immune disease in which various cytokines, primarily tumor necrosis factor- (TNF-), are complexly involved. Mannose-binding lectin (MBL) gene polymorphisms decrease MBL serum levels, thereby increasing the synthesis of proinflammatory cytokines such as TNF-. Objectives This trial was designed to evaluate the role of the MBL2 codon 54 polymorphism in the pathogenesis of psoriasis. Methods Fifty patients diagnosed with psoriasis vulgaris and 53 healthy subjects were included in the trial. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was applied to determine the MBL2 codon 54 polymorphism. Genotypes were determined according to the bands formed in agarose electrophoresis gels. For the statistical analysis, the level of significance was set at P<0.05. Results A total of 33 (66.0%) of the 50 psoriasis patients were detected to have A/A genotype and 17 (34.0%) had B/B genotype. Of the control subjects, 44 (83.0%) had A/A genotype and nine (17.0%) had B/B genotype. There was a statistically significant difference between the groups (P=0.047). The analysis of allele frequencies revealed A allele prevalences to be 79 (79.0%) and 95 (89.6%), and B allele prevalences to be 21 (21.0%) and 11 (10.4%), in the patient and control groups, respectively. A statistically significant difference between allele frequencies was detected (P=0.031). Conclusions This study suggests that the MBL2 codon 54 polymorphism may have an association with psoriasis in the Turkish population
Obstrüktif uyku apne sendromu olan türk hastalarda TNF-alfa G308A ve C857T gen polimorfizmlerinin incelenmesi
No full textObjective: Tumor necrosis factor-alpha (TNF-alpha) is an important indicator of inflammation. Recent studies have demonstrated a relationship between inflammation and obstructive sleep apnea syndrome (OSAS). The aim of this study was to investigate the association between TNF-alpha G308A and C857T gene polymorphisms and OSAS in Turkish patients. Material and Methods: Sixty-nine patients who were diagnosed with OSAS and 42 control subjects were included in the study. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to detect TNF-alpha G308A and C857T gene polymorphisms. The level of significance for statistical analysis was set at p 0.05). However, the mean body mass index of the OSAS group was significantly different from that of the control group (p<0.05). Conclusion: To our knowledge, this study is the first to analyze the relationship between OSAS and INF-alpha G308A and C857T gene polymorphisms in Turkish patients. Our results do not support an association between OSAS and TNF-alpha G308A and C857T gene polymorphisms
Investigation of tnf-alpha gene (g308a) and gstp1 gene (ile105val) polymorphisms in Turkish patients with retinopathy of prematurity
No full textBackground/aim: Retinopathy of prematurity (ROP) is one of the most frequent causes of blindness in newborn babies. Currently, its etiology is not fully understood. In this study we aimed to investigate the correlation between a patient group with ROP and a control group in terms of the tumor necrosis factor- alpha (TNF-alpha) (G308A) gene and glutathione-S-transferase P1 (GSTP1) (Ile105Val) gene polymorphism.Materials and methods: Sixty-two patients diagnosed with ROP and 58 control subjects were included in this study. For TNF-alpha (G308A) gene and GSTP1 (Ile105Val) gene polymorphisms, the polymerase chain reaction- restriction fragment length polymorphism method was used. In statistical analysis the significance level was determined as P 0.05).Conclusion: In our study, no correlation was identified between TNF-alpha (G308A) gene and GSTP1 (Ile105Val) gene polymorphisms and susceptibility for development of ROP. Further studies are required with more cases of ROP patients and other gene polymorphisms that could be related
Investigation of GSTP1 (Ile105val) gene polymorphlsm ln chronic myelold leukaemla patlents
No full textBazı kanser türlerinde yatkınlık ile Faz II detoksifikasyon reaksiyonlarında yer alan Glutatyon-S-Transferaz(GST) enzimi genlerinin polimorfizmleri arasında ilişki gösterilmiştir. Bu çalışmada, kronik myeloid lösemi (KML) gelişimin ile GSTP1 (lle105Val) gen polimorfizmi arasındaki ilişkiyi araştırmayı amaçladık.Çalışmamıza KML tanısı almış 71 hasta ve herhangi bir kanser hikayesi olmayan 67 kişi alındı. GSTP1 (lle105Val) gen polimorfizmi için polimeraz zincir reaksiyonu-restriksiyon fragment uzunluk polimorfizmi (polymerase chain reaction-restriction fragment length polymorphism=PCR-RFLP) yöntemi uygulandı. Agaroz jeldeki bantlara göre genotipler belirlendi. İstatistiksel analizde p0.05).Bulgularımız GSTP1 (IIe105Val) gen polimorfizmi ile KML arasında bir ilişki olmadığını göstermiştir. Bununla birlikte daha geniş olgu serilerinde bu sonuçlar desteklenmelidir.Associations between polymorphisms for genes encoding Glutathione S-transferases (GST) enzymes involved in Phase II detoxification reactions and susceptibility to some cancers have been shown in several studies. The aim of the present study is to investigate the influence of Glutathione S-transferases P1 (IIe105Val) gene polymorphism in susceptibility to chronic myeloid leukaemia (CML).71 CML patients and 67 control subjects with no cancer history were enrolled in our study. PCR-restriction fragment length polymorphism (PCR-RFLP) method was used for GSTP1 (lle105Val) gene polymorphism. Genotypes were determined according to the bands that formed in agarose electrophoresis gels. In statistical analysis, the level of significance was set at p0.05).Our results showed that there was not any association between GSTP1 (IIe105Val) gene polymorphism and chronic myeloid leukaemia. However, these findings should be confirmed in studies with larger population
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Investigaçao de polimorfismos no gene MEFV (G138G e A165A) em pacientes adultos com febre mediterranica familiar
No full textAim: Various mutations have been identified in the Mediterranean fever (MEFV) gene which is reported to be responsible from Familial Mediterranean fever (FMF). In our study, we aimed to determine the frequency of the MEFV mutations in our region and to investigate the impact of G138G (rs224224, c.414A>G) and A165A (rs224223, c.495C>A) gene polymorphisms on the clinical findings of the disease. Methods: One hundred and sixteen patients diagnosed with FMF and 95 control subjects were included in this study. We used the DNA sequence analysis method to identify the most prevailing 10 mutations located in exon 2 and 10 of MEFV gene. Results: As a result of the MEFV mutation analysis, the most common mutation was the M694V mutation allele with a frequency rate of 41.8%. When the patients group and control group were compared in terms of frequency of both polymorphic alleles (G polymorphic allele, observed in G138G and the A polymorphic allele, observed in A165A), the variation was observed to be statistically significant (p 0.05). Conclusions: To our knowledge, our study is the first study in the Southern Marmara region that reports the frequency of MEFV mutations. Our findings imply that the polymorphisms of G138G and A165A may have an impact on progress of the disease. We think that more studies, having higher number of cases and investigating the polymorphisms of MEFV gene, are needed.Resumo Objetivo: Identificaram-se mutações no gene da febre mediterrânica (MEFV) relatadas como responsáveis pela febre mediterrânica familiar (FMF). Este estudo teve como objetivo determinar a frequência de mutações no MEFV na região sul do mar de Mármara e investigar o impacto dos polimorfismos genéticos G138G (rs224224, c.414A > G) e A165A (rs224223, c.495C > A) nos achados clínicos da doença. Métodos: Foram incluídos neste estudo 116 pacientes com diagnóstico de FMF e 95 indivíduos no grupo controle. Usou-se o método de análise da sequência de DNA para identificar as 10 mutações mais prevalentes localizadas nos éxons 2 e 10 do gene MEFV. Resultados: Como resultado da análise da mutação MEFV, a mutação mais comum foi a mutação alélica M694 V, com uma taxa de frequência de 41,8%. Quando os grupos de pacientes e controles foram comparados em termos de frequência de ambos os alelos polimórficos (alelo polimórfico G, observado no G138G e o alelo polimórfico A, observado no A165A), a variação observada foi estatisticamente significativa (p 0,05). Conclusões: Que se tem conhecimento, este estudo é o primeiro feito na região sul do mar de Mármara que relata a frequência de mutações no MEFV. Os achados indicam que os polimorfismos G138G e A165A podem ter um impacto sobre o progresso da doença. Acredita-se que são necessários mais estudos que abranjam um maior número de casos e investiguem os polimorfismos do gene MEFV
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