76 research outputs found
Analytical workflow integrating LC-HRMS untargeted analysis and CyanoMetDB for fast and extensive detection of cyanobacterial metabolites
No full textCyanobacteria produce a large number of secondary metabolites including cyanotoxins and a variety of bioactive peptides with great structural diversity. Identification of these metabolites (cyanometabolites) is a great analytical challenge due to the extremely limited availability of analytical standards and lack of a well-documented fragmentation mass spectra database. In the present study, an analytical workflow was developed for the detection of the cyanometabolites in bloom samples from Greek lakes. Samples were extracted [1,2] and analyzed by LC-HRMS (Orbitrap Fusion Lumos Tribrid MS) in data depended acquisition (DDA) mode. Fragmentation spectra of compounds were obtained with collision-induced dissociation (CID) and higher-energy C-trap dissociation (HCD) modes. Acquired data were processed with Compound Discoverer software in combination to the recently published CyanoMetDB mass list [3] and other related tools for the annotation and structural elucidation of cyanometabolites. Verification of proposed structures was performed based on in silico fragmentation and fragment ion search (FISh) scoring. Application of the workflow revealed the presence of numerous congeners belonging to the cyanotoxins class microcystins and to the understudied cyanopeptides classes of cyanopeptolines, microginins, aeruginosins, anabaenopeptins and aeruginosamides. Furthermore, new congeners were annotated clearly demonstrating the suitability of the approach for the characterization of cyanobacterial chemodiversity.
[1] C. Christophoridis, S.-K. Zervou, K. Manolidi, M. Katsiapi, M. Moustaka-Gouni, T. Kaloudis, T. M. Triantis and A. Hiskia, Scientific Reports 8 (2018) 17877.
[2] S.-K. Zervou, K. Moschandreou, A. Paraskevopoulou, C. Christophoridis, E. Grigoriadou, T. Kaloudis, T. M. Triantis, V. Tsiaoussi and A. Hiskia, Toxins 13 (2021) 394.
[3] M. R. Jones, E. Pinto, M. A. Torres, F. Dörr, H. Mazur-Marzec, K. Szubert, L. Tartaglione, C. Dell’Aversano, C. O. Miles, D. G. Beach, P. McCarron, K. Sivonen, D. P. Fewer, J. Jokela and E. M.-L. Janssen, Water Research 196 (2021) 117017
Quantitative Screening of Microcystins and Nodularin in Water Samples with Commercially Available ELISA Kits
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Foreword to the Themed Issue “Cyanobacteria”
No full textCOST (European Cooperation in Science and Technology) is a pan-European intergovernmental framework. Its mission is to enable break-through scientific and technological developments leading to new concepts and products and thereby contribute to strengthening Europe’s research and innovation capacities. It allows researchers, engineers and scholars to jointly develop their own ideas and take new initiatives across all fields of science and technology, while promoting multi- and interdisciplinary approaches. COST aims at fostering a better integration of less research-intensive countries to the knowledge hubs of the European Research Area. The COST Association, an International Not-for-profit Association under Belgian Law, integrates all management, governing and administrative functions necessary for the operation of the framework. The COST Association has currently 36 Member Countries. www.cost.e
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