1,721,022 research outputs found
Facilitating the exploitation of Linked Open Statistical Data: JSON-QB API requirements and design criteria
No full textRecently, many organizations have opened up their data for others to reuse. A major part of these data concern statistics such as demographic and social indicators. Linked Data is a promising paradigm for opening data because it facilitates data integration on the Web. Recently, a growing number of organizations adopted linked data paradigm and provided Linked Open Statistical Data (LOSD). These data can be exploited to create added value services and applications that require integrated data from multiple sources. In this paper, we suggest that in order to unleash the full potential of LOSD we need to facilitate the interaction with LOSD and hide most of the complexity. Moreover, we describe the requirements and design criteria of a JSON-QB API hat (i) facilitates the development of LOSD tools through a style of interaction familiar to web developers and (ii) offers a uniform way to access LOSD. A proof of concept implementation of the JSON-QB API demonstrates part of the proposed functionality.Joint Proceedings of the International Workshops on Hybrid Statistical Semantic Understanding and Emerging Semantics, and Semantic Statistics co-located with 16th Extended Semantic Web Conference (ISWC 2017
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Investigation of metabolization pathways of bioactive molecules of edible, medicinal plants and their products in various in vitro and in vivo systems. Isolation and identification of metabolites thereof.
No full textΗ παρούσα διατριβή διερευνά τις μεταβολικές οδούς βιοδραστικών μορίων από βρώσιμα, φαρμακευτικά φυτά και τους μεταβολίτες τους σε διάφορα in vitro και in vivo συστήματα, με ιδιαίτερη έμφαση στις βιοφαινόλες της ελιάς και τα σεσκιτερπένια του κουρκουμά. Η κατανόηση των μηχανισμών δράσης των βιοενεργών μορίων των τροφίμων (FBs) και του ρόλου τους στην προαγωγή της υγείας απαιτεί λεπτομερή διαλεύκανση των μεταβολικών μονοπατιών τους, ξεκινώντας από την ακριβή ταυτοποίηση των μεταβολιτών τους. Για να επιτευχθεί αυτό, χρησιμοποιούνται συνήθως προηγμένες αναλυτικές πλατφόρμες, όπως LC/GC-MS υψηλής διακριτικής ικανότητας και NMR. Το κρίσιμο βήμα της ταυτοποίησης των μεταβολιτών ή των βιοδεικτών εξαρτάται σε μεγάλο βαθμό από τις βάσεις δεδομένων και την υπάρχουσα βιβλιογραφία. Ωστόσο, η σημαντική δομική ποικιλομορφία των FBs και των μεταβολιτών τους, σε συνδυασμό με την έλλειψη προτύπων αναφοράς, καθώς και το γεγονός ότι οι ενώσεις αυτές είναι συχνά παρούσες στα ανθρώπινα βιολογικά υγρά σε πολύ χαμηλές συγκεντρώσεις, καθιστούν την ακριβή ταυτοποίηση και τον ποσοτικό προσδιορισμό τους σημαντική αναλυτική πρόκληση. Για την αντιμετώπιση αυτών των προκλήσεων, η απομόνωση των μεταβολιτών ενδιαφέροντος σε καθαρή μορφή από τα ανθρώπινα ούρα ή άλλα υποστρώματα, αποτελεί μια πολλά υποσχόμενη εναλλακτική λύση για την επίτευξη αδιαμφισβήτητης ταυτοποίησης. Ο καθαρισμός σε κλίμακα milligramm όχι μόνο θα παρείχε ολοκληρωμένα φασματικά δεδομένα από LC-MS καθώς και κρίσιμες πληροφορίες όπως η χρωματογραφική συμπεριφορά και η πολικότητα, αλλά θα επέτρεπε επίσης πειράματα 1D και 2D NMR. Η παρούσα διατριβή διαρθρώνεται σε τέσσερα κεφάλαια, καθένα από τα οποία ασχολείται με μια συγκεκριμένη πτυχή της προετοιμασίας του δείγματος, της παρασκευαστικής απομόνωσης των μεταβολιτών των FBs και της βιολογικής αξιολόγησης αυτών των ενώσεων, καθώς και της ανάλυσης των βιοχημικών μονοπατιών. Ο απώτερος στόχος είναι να ενισχύσουμε την κατανόηση του τρόπου με τον οποίο τα βιοδραστικά μόρια από φαρμακευτικά φυτά ή τρόφιμα μεταβολίζονται στον ανθρώπινο οργανισμό και τις πιθανές εφαρμογές τους στην εξατομικευμένη διατροφή και την πρόληψη ασθενειών.This thesis explores the metabolic pathways of bioactive molecules from edible, medicinal plants and their products in various in vitro and in vivo systems, with a particular focus on olive biophenols and turmeric sesquiterpenes. Understanding of the mechanisms of action of food bioactives (FBs) and their role in promoting health requires detailed elucidation of their metabolic pathways, beginning with the precise identification of their metabolites. To achieve this, advanced analytical platforms such as high-resolution LC/GC-MS and NMR are typically employed. The critical step of identifying metabolites or biomarkers is largely dependent on databases and existing literature. However, the substantial structural diversity of FBs and their metabolites, coupled with the scarcity of reference standards, as well as the fact that these compounds are often present in human biological fluids at very low concentrations makes their precise identification and quantification a significant analytical challenge. To address these challenges, the preparative isolation of metabolites of interest in pure form from human urine or other substrates, presents a promising alternative for achieving unambiguous identification. Purification on a milligram scale would not only provide comprehensive spectral data from LC-MS as well as critical information such as chromatographic behavior and polarity but also allows 1 and 2D NMR experiments. The present thesis is structured into four chapters, each addressing a specific aspect of the sample preparation, preparative isolation of FBs metabolites and biological evaluation of these compounds, as well as biochemical pathways analysis. The overarching goal is to advance our understanding of how the bioactive compounds from medicinal plants or foods are metabolized in the human body and their potential applications in personalized nutrition and disease prevention. Chapter 1 focuses on the development of a novel multiscale workflow for the preparative isolation of FBs-related metabolites from human urine by embedding established chromatographic tools from the field of natural products chemistry and the unambiguous structural characterization thereof. Chapter 1 is divided into two parts. Ιn part I, the primary aim was to develop a unified urine pretreatment workflow that is compatible with typical profiling techniques while also allowing for the isolation of biotransformation products and metabolites, contributing to their unambiguous identification. Towards this purpose, hydroxytyrosol (ΗΤ) was supplemented as a soft capsule (15 mg/day) to a healthy volunteer for one month and 24-h samples were collected. Typical analytical protocols used for biological samples pre-treatment were applied together with enrichment methodologies which are employed in natural products research. All derived extracts were analyzed via HPLC-DAD/ELSD, UPLC-HRMS and NMR to investigate their chemical profile and evaluated in terms of recovery yield, metabolite richness, and specificity based on the chemical classes of identified metabolites. Ultimately, the proposed methodology was successfully applied for large-volume urine pretreatment, offering the potential for metabolite isolation. The novelty of the current work lies in the development of a comprehensive workflow that facilitates both analytical profiling and the isolation of urine metabolites. To the best of our knowledge, such an integrated concept has not been reported in literature before. Another innovative aspect of this approach is the use of multiple analytical platforms in combination, providing complementary results for highly accurate metabolite identification. It is important to highlight that both qualitative and quantitative assessments were performed, focusing on the number of identified metabolites, their chemical classes, and their related biochemical pathways. To our knowledge, no similar study has investigated the efficiency of a urine pretreatment method while considering these aspects, which are crucial for accurate and complete interpretation of results towards biochemical pathways analysis and biological role interpretation. In Part II we tried to develop a novel method for the separation, isolation and identification of FBs metabolites from human urine as well as to investigate their biochemical role through molecular pathways analysis. The opportunity of recognizing and quantifying the pathways in which FBs metabolites are involved will allow us to understand in depth their mechanisms of action and the interactions that may exert their beneficial effect. A major prerequisite in unraveling the underlying mechanisms is either organic synthesis or preparative isolation of these metabolites, which hampers their structural elucidation and biological assessment. Isolation of metabolites from human urine can be a useful alternative source for the recovery of bioactive compounds and an important driving force in drug discovery process since urine encompasses a wide range of chemical classes (including gut microbial metabolites and xenobiotics), it may be collected in large volumes non-invasively, making it an ideal starting material for purification, and is rich in small molecule metabolites and has a very low concentration in macromolecules. Using hydroxytyrosol (HT) as a case study, part II outlines a novel methodology for isolating and characterizing FB metabolites from human urine by embedding established chromatographic tools from the field of natural products chemistry to biological samples. Our approach could be used for the discovery of unknown metabolites that are not present in experimental databases, contributing to their full characterization. We believe that the proposed protocol will be valuable not only in traditional urine metabolomic studies but also in cases where unambiguous identification of metabolites is crucial, particularly in the analysis of biochemical pathways and the investigation of FB mechanisms of action within the framework of personalized nutrition. Chapter 2 shifts focus on the biotechnological production of hydroxytyrosol using E. coli biofactories. Microbial production of bioactive molecules provides a promising alternative to traditional isolation or chemical synthesis approaches, enabling high-yield production of pure compounds without the need for heavy-metal catalysts, harsh conditions, or complex plant-based processes. E. coli has been widely used to synthesize diverse natural bioactive compounds. While microbial factories have been effective for HT production, optimization of yield and recovery process remain a challenge. Furthermore, isolation of HT and its metabolites continues to suffer from inefficiencies during extraction, separation, and purification even when biosynthetic pathways are successful. The chapter addresses the challenges associated with the biotechnological production, extraction and purification of HT from fermentation broths, which are often complex and contain impurities. To overcome these challenges, the chapter introduces a systematic multistep protocol that incorporates centrifugal extraction and partition chromatographic techniques, such as Annular Centrifugal Extraction (ACE) and Centrifugal Partition Chromatography (CPC). These methods enable the efficient isolation and purification of HT and its metabolites, facilitating their structural elucidation using HRMS/MS and NMR. This innovative approach not only optimizes the production of HT but also provides insights into the metabolic pathways of engineered microorganisms. This can significantly aid in pathway optimization by enabling efficient monitoring of the process and optimizing metabolic flux. This methodology aims to generate new insights into the mechanistic understanding of microbial metabolic biotransformations during fermentation but also to introduce centrifugal chromatography methodologies in biotechnological workflows. In Chapter 3 the aim was to evaluate the activity of the olive oil biophenols (OBs) isolated in the preceding two chapters utilizing two model organisms: Caenorhabditis elegans and zebrafish. Part A investigates the modulatory role of OBs on increasing lifespan, stress resistance and the pathogenesis of Parkinson's disease in C. elegans experimental model. The study demonstrates that OBs, particularly HT and oleuropein, can modulate oxidative stress and reduce the aggregation of α-synuclein, a key factor in PD. Part B explores the anticonvulsant activity of OBs in a zebrafish (Danio rerio) model of epilepsy. The findings suggest that OBs, such as oleuropein and HT, exhibit protective effects against seizures, highlighting their potential as novel therapeutic agents for neurological disorders. Chapter 4 examines the metabolic fate of bisabolane sesquiterpenes derived from turmeric essential oil (TEO) in the human body. Despite the well-documented health benefits of TEO, the bioavailability and metabolism of its sesquiterpenes remain poorly understood. Structural modifications in ΤEΟ bioactives are expected to result in different and potentially improved biological effects. This chapter proposes a multiplatform analytical approach combining Centrifugal Partition Chromatography (CPC), UHPLC-Orbitrap-MS, NMR, and GC/MS for the profiling, isolation and structural elucidation of TEO metabolites. The multiplatform approach provides a clear advantage to expanding the coverage of metabolome and improves our confidence in metabolite identification. The study aims to uncover novel metabolites with enhanced pharmacological activity, providing new insights into the metabolic pathways of turmeric sesquiterpenes and their potential health benefits. In summary, this thesis contributes to the growing body of knowledge on the metabolism and bioactivity of natural bioactive compounds. By developing innovative methodologies for metabolite isolation and characterization, the research provides a deeper understanding of how these compounds exert their health benefits. The findings have significant implications for the investigation of metabolic pathways of medicinal plants in human organism and the development of novel therapeutic agents derived from natural sources
Ανάπτυξη μεθόδου ποσοτικού προσδιορισμού ολικής trans - ρεσβερατρόλης σε οίνους με χρήση φασματοσκοπίας NMR
No full textΗ trans-ρεσβερατρόλη είναι μια φαινολική ένωση, που ανήκει στην οικογένεια των στιλβενίων, η οποία βρίσκεται σε διάφορα μέρη του σταφυλιού, συμπεριλαμβανομένου και του φλοιού της ράγας. Η ρεσβερατρόλη ανήκει σε μια ευρύτερη κατηγορία ενώσεων που έχουν χαρακτηριστεί ως φυτοαλεξίνες, αφού παράγονται σε συνθήκες βιοτικού και αβιοτικού στρες, όπως για παράδειγμα οι προσβολές από μικροοργανισούς ή οι κακώσεις των ιστών από την υπεριώδη ακτινοβολία, ενώ μεταφέρονται κατά τη διάρκεια της οινοποίησης στο γλεύκος και στον οίνο. Η ρεσβερατρόλη παρουσιάζει ισχυρή αντιοξειδωτική δράση, δηλαδή εξουδετερώνει τις οξυγονούχες ρίζες και οξειδωτικές ενώσεις που παράγονται στους διάφορους ιστούς ή κυκλοφορούν στο πλάσμα του αίματος και αποτρέπει την οξείδωση των μετάλλων στο σώμα. Από την άποψη αυτή η ρεσβερατρόλη διαθέτει καρδιοπροστατευτικές ιδιότητες. Επίσης σύμφωνα με νεότερες μελέτες, η ρεσβερατρόλη φαίνεται ότι προστατεύει και από την ανάπτυξη κακοηθών όγκων, εμποδίζει την εμφάνιση και την ανάπτυξη καρκίνου του δέρματος και έχει αντιφλεγμονώδη δράση. Στην παρούσα εργασία αναπτύχθηκε μια πρωτότυπη και γρήγορη μέθοδος ποσοτικού προσδιορισμού της ολικής trans-ρεσβερατρόλης (πιο συγκεκριμένα των ολικών παράγωγων trans-ρεσβερατρολοειδών) σε οίνους με τη χρήση φασματοσκοπίας ΝΜR. Στη συνέχεια αναλύθηκαν 10 δείγματα κρασιών βιομηχανικής παραγωγής των ενδογενών (Π.Ο.Π.) ερυθρών ποικιλιών Αγιωργίτικο, Ξινόμαυρο, Φωκιανό, Λιάτικο, Κοτσιφάλι, Μαυροδάφνη, Λημνιό και Μοσχάτο Τυρνάβου και προσδιορίστηκε το περιεχόμενο των ολικών ρεσβερατρολοειδών σ’ αυτά. Οι μετρήσεις αυτές έδειξαν ότι την μεγαλύτερη περιεκτικότητα σε ρεσβερατρόλη είχαν το Κοτσιφάλι, το Ξινόμαυρο και το Αγιωργίτικο, δεδομένο το οποίο επαληθεύεται και από παλαιότερες επιστημονικές δημοσιεύσεις.Trans-resveratrol is a phenolic compound belonging to the family of stilbene, which is found in various parts of the grape. Resveratrol belongs to a broader class of compounds that have been identified as phytoalexins, since they are produced in living and abiotic stress conditions, such as microorganisms attacks or tissue damage from ultraviolet radiation, while being transported during the vinification to the must and the wine. Resveratrol has a strong antioxidant effect, ie neutralizes oxygenated roots and oxidative compounds produced in the various tissues or circulating in blood plasma and prevents oxidation of metals in the body. In this respect, resveratrol has cardioprotective properties. According to newer studies, resveratrol appears to protect against the development of malignant tumors, inhibit the appearance and development of skin cancer and have anti-inflammatory properties In this study we developed a novel and rapid method of quantifying the total trans-resveratrol content (in particular of total trans-resveratrolloids) in wines using NMR spectroscopy. Subsequently, 10 samples of the local red P.O.P. varieties Agiorgitiko, Xinomavro, Fokianos, Liatiko, Kotsifali, Mavrodaphni, Limnio and Moschato were analyzed and the content of total resveratrolloids was determined. These measurements showed that the highest content of resveratrol had Kotsifali, Xinomavro and Agiorgitiko, a fact also verified in older scientific publications
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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