1,720,963 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Epigenetic Regulations and Promoter Characterization of CERIG (Cancer Endoplasmic Reticulum Gene-KIAA1199)
Get PDFAlthough human genome project is complete, the biological function of most genes remains obscure. In this thesis project, the regulatory mechanisms of a novel endoplasmic reticulum (ER) resident protein identified by a PCR-subtraction hybridization technique, designated as Cancer ER Invasion Gene (CERIG), is investigated. CERIG is found to be highly expressed in human invasive breast cancer specimens examined by immunohistochemistry and real-time RT-PCR. This up-regulation of CERIG is associated with poor prognosis of patients with breast cancer assessed by a DNA microarray data-mining approach from three publicly available cohorts containing a total of 696 breast cancer patients. In study of the regulation mechanism of CERIG, a 3.3 kb fragment of human genomic DNA containing the 5\u27-flanking sequence of the CERIG is found to possess both suppressive and activating elements. Employing a deletion mutagenesis approach, a 1.4 kb proximal region is defined as the basic CERIG promoter containing a TATA-box close to the transcription start site. A combination of 5\u27-primer extension approach with a bioinformatics analysis from the Cap-Analysis Gene Expression database reveals a single transcription start site in the human CERIG gene. Bioinformatics analysis suggests that the 1.4 kb CERIG promoter contains putative activating regulatory elements, including activator protein-1(AP-1), Twist-1, and NF-?ÂB sites. Sequential deletion and site-direct mutagenesis analysis demonstrate that the AP-1 and distal NF-?ÂB sites are required for CERIG gene expression. Further analyses using an electrophoretic mobility-shift assay and chromatin immunoprecipitation confirmed the requirement of these cis- and trans-acting elements in controlling CERIG gene expression. In further analysis of the regulatory mechanism of CERIG in cancer progression, we examine whether CERIG mRNA expression is regulated by the DNA methylation and/or histone H3 modification. Aberrant DNA methylation of CERIG 5\u27-untranslated region is identified as an important regulatory mechanism in the deregulation of CERIG. In vitro promoter methylation experiments and the demethylating reagent, 5\u27azacytidine, are used to determine the role of DNA methylation in CERIG expression. Pyrosequencing analysis revealed demethylation of CERIG in human breast cancer specimen that correlated with high expression of CERIG. The role of chromatin modifications in CERIG expression is also determined. Correlation between the level of tri-methylated H3K4 (activation marker) and the higher level of CERIG expression has been demonstrated in breast cancer cells. Substitution of H3K4me3 with H3K27me3 on histone tail has been found in cancer cells or primary cell lines in which CERIG expression declines. Additionally, the effects of hypoxia was analyzed on both epigenetic mechanisms for CERIG expression and determined that hypoxia increases the activation marker and decreases the repression marker on the CERIG promoter without changing the level of DNA methylation. Taken together, this study uncovers the regulatory mechanism of CERIG in breast cancer progression and suggests that CERIG may be used as a prognostic marker and potential therapeutic target in prevention of cancer metastasis. | 132 page
Epigenetic Regulations and Promoter Characterization of CERIG (Cancer Endoplasmic Reticulum Gene-KIAA1199)
Get PDF132 pg.Although human genome project is complete, the biological function of most genes remains obscure. In this thesis project, the regulatory mechanisms of a novel endoplasmic reticulum (ER) resident protein identified by a PCR-subtraction hybridization technique, designated as Cancer ER Invasion Gene (CERIG), is investigated. CERIG is found to be highly expressed in human invasive breast cancer specimens examined by immunohistochemistry and real-time RT-PCR. This up-regulation of CERIG is associated with poor prognosis of patients with breast cancer assessed by a DNA microarray data-mining approach from three publicly available cohorts containing a total of 696 breast cancer patients. In study of the regulation mechanism of CERIG, a 3.3 kb fragment of human genomic DNA containing the 5'-flanking sequence of the CERIG is found to possess both suppressive and activating elements. Employing a deletion mutagenesis approach, a 1.4 kb proximal region is defined as the basic CERIG promoter containing a TATA-box close to the transcription start site. A combination of 5'-primer extension approach with a bioinformatics analysis from the Cap-Analysis Gene Expression database reveals a single transcription start site in the human CERIG gene. Bioinformatics analysis suggests that the 1.4 kb CERIG promoter contains putative activating regulatory elements, including activator protein-1(AP-1), Twist-1, and NF-?ÂB sites. Sequential deletion and site-direct mutagenesis analysis demonstrate that the AP-1 and distal NF-?ÂB sites are required for CERIG gene expression. Further analyses using an electrophoretic mobility-shift assay and chromatin immunoprecipitation confirmed the requirement of these cis- and trans-acting elements in controlling CERIG gene expression. In further analysis of the regulatory mechanism of CERIG in cancer progression, we examine whether CERIG mRNA expression is regulated by the DNA methylation and/or histone H3 modification. Aberrant DNA methylation of CERIG 5'-untranslated region is identified as an important regulatory mechanism in the deregulation of CERIG. In vitro promoter methylation experiments and the demethylating reagent, 5'azacytidine, are used to determine the role of DNA methylation in CERIG expression. Pyrosequencing analysis revealed demethylation of CERIG in human breast cancer specimen that correlated with high expression of CERIG. The role of chromatin modifications in CERIG expression is also determined. Correlation between the level of tri-methylated H3K4 (activation marker) and the higher level of CERIG expression has been demonstrated in breast cancer cells. Substitution of H3K4me3 with H3K27me3 on histone tail has been found in cancer cells or primary cell lines in which CERIG expression declines. Additionally, the effects of hypoxia was analyzed on both epigenetic mechanisms for CERIG expression and determined that hypoxia increases the activation marker and decreases the repression marker on the CERIG promoter without changing the level of DNA methylation. Taken together, this study uncovers the regulatory mechanism of CERIG in breast cancer progression and suggests that CERIG may be used as a prognostic marker and potential therapeutic target in prevention of cancer metastasis.Advisor(s): Cao, Jian . Committee Member(s): Fleit, Howard B; Gergen, Peter J; Zucker, Stanley.Stony Brook University Libraries. SBU Graduate School in Department of Molecular and Cellular Biology. Charles Taber (Dean of Graduate School)
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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