1,794,028 research outputs found
tvwenger/kd: Initial Release
No full text<p>Utilities to calculate kinematic distances</p>
<p>Latest code on Github: <a href="https://github.com/tvwenger/kd">https://github.com/tvwenger/kd</a></p>
<p>On-line tool available here: <a href="http://www.treywenger.com/kd/">http://www.treywenger.com/kd/</a></p>
ABC of Kd Accuracy
No full textThe equilibrium dissociation constant (Kd) is a major characteristic of affinity complexes and one of the most frequently determined physicochemical parameters. Despite its significance, the values of Kd obtained for the same complex under similar conditions often exhibit considerable discrepancies and sometimes vary by orders of magnitude. These inconsistencies highlight the susceptibility of Kd determination to large systematic errors, ultimately leading to misconceptions and significant misallocation of research and development resources. It is imperative to both minimize and quantitatively assess the systematic errors inherent in Kd determination. Traditionally, Kd values are determined through nonlinear regression of binding isotherms. This analysis utilizes three variables: concentrations of two reactants and a fraction R of unbound limiting reactant. The systematic errors in Kd arise directly from systematic errors in these variables. In this study, we thoroughly analyze the sources of systematic errors within these variables, aiming to mitigate their impact on Kd accuracy. Through this analysis, we illustrate how each source contributes to inaccuracies in Kd determination. Additionally, we propose a method for quantitatively assessing the confidence interval of systematic errors in concentrations, a crucial step towards quantitatively evaluating Kd accuracy. While presenting original findings, this paper also reiterates the fundamentals of Kd determination, hence, guiding researchers across all proficiency levels. By shedding light on the sources of systematic errors and offering strategies for their mitigation, our work will help researchers to enhance the accuracy of Kd determination thereby making binding studies more reliable
02 HAIKU [05:33] (KUG KD 63)
No full textKomposition: Filippo Minisola (Italy)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp3Komposition: Filippo Minisola (Italy)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp
04 POINTING TO THE SKY [06:41] (KUG KD 63)
No full textKompostition: Gabriele Ceccarelli (Italy)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp3Kompostition: Gabriele Ceccarelli (Italy)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp
06 INTO ETERNITY [08:24] (KUG KD 63)
No full textKomposition: Suzan Veneman (The Netherlands)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp3Komposition: Suzan Veneman (The Netherlands)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp
03 THE TIDE TURNS [07:24] (KUG KD 63)
No full textKomposition: Henri Lyysaari (Finland)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp3Komposition: Henri Lyysaari (Finland)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp
01 THE PROPERTIES OF RUBBER [08:52] (KUG KD 63)
No full textKomposition: Joan Pérez-Villegas (Spain)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp3Komposition: Joan Pérez-Villegas (Spain)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp
Native PAGE experiments on SL1-wt RNA kissing dimer (KD) in the presence of sub-stoichiometric amounts of NCp7, run in the TBE buffer
No full text<p><b>Copyright information:</b></p><p>Taken from "Nucleocapsid protein-mediated maturation of dimer initiation complex of full-length SL1 stemloop of HIV-1: sequence effects and mechanism of RNA refolding"</p><p></p><p>Nucleic Acids Research 2007;35(6):2026-2034.</p><p>Published online 6 Mar 2007</p><p>PMCID:PMC1874624.</p><p>© 2007 The Author(s)</p> KD of SL1-wt was incubated with NCp7 at ambient temperature for 18 h in the RNA strand-to-protein ratio of 2:0, 2:2, 2:1.6, 2:0.8, 2:0.4 and 2:0.2, as indicated. The upper band in each lane (‘LD’) corresponds to mature linear dimer, while the lower monomer band (‘M’) emanates from the residual KD that dissociates during PAGE in the TBE buffer. Increasing intensity of the monomer band and simultaneous decrease in the dimer band across the lanes correlates with the amount of NCp7 present in each complex
10 THE WOODPECKER [07:26] (KUG KD 63)
No full textKomposition: Charlie Bates (Great Britain)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp
09 THE PATH [07:49] (KUG KD 63)
No full textKomposition: Alessio Sacco (Italy)
Album: Jazz Comp Graz 2020; 2022 (KUG KD 63, 1 CD)
Format: mp
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