19,364 research outputs found
First person – Osvaldo Contreras
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Osvaldo Contreras is first author on ‘Cross-talk between TGF-β and PDGFRα signaling pathways regulates the fate of stromal fibro–adipogenic progenitors’, published in JCS. Osvaldo is a postdoctoral scientist in the laboratory of Enrique Brandan at Departamento de Biología Celular y Molecular and Center for Aging and Regeneration (CARE-ChileUC), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile, investigating skeletal muscle tissue-resident mesenchymal progenitors in health and disease
The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells
No full textThe polarized distribution of Na+,K+-ATPase plays a paramount physiological role, because either directly or through coupling with co- and countertransporters, it is responsible for the net movement of, for example, glucose, amino acids, Ca2+, K+, Cl-, and CO3H- across the whole epithelium. We report here that the beta-subunit is a key factor in the polarized distribution of this enzyme. 1) Madin-Darby canine kidney (MDCK) cells (epithelial from dog kidney) express the Na+,K+-ATPase over the lateral side, but not on the basal and apical domains, as if the contact with a neighboring cell were crucial for the specific membrane location of this enzyme. 2) MDCK cells cocultured with other epithelial types (derived from human, cat, dog, pig, monkey, rabbit, mouse, hamster, and rat) express the enzyme in all (100%) homotypic MDCK/MDCK borders but rarely in heterotypic ones. 3) Although MDCK cells never express Na+,K+-ATPase at contacts with Chinese hamster ovary (CHO) cells, they do when CHO cells are transfected with beta(1)-subunit from the dog kidney (CHO-beta). 4) This may be attributed to the adhesive property of the beta(1)-subunit, because an aggregation assay using CHO (mock-transfected) and CHO-beta cells shows that the expression of dog beta(1)-subunit in the plasma membrane does increase adhesiveness. 5) This adhesiveness does not involve adherens or tight junctions. 6) Transfection of beta(1)-subunit forces CHO-beta cells to coexpress endogenous a-subunit. Together, our results indicate that MDCK cells express Na+,K+-ATPase at a given border provided the contacting cell expresses the dog P,-subunit. The cell-cell interaction thus established would suffice to account for the polarized expression and positioning of Na+,K+-ATPase in epithelial cells
Historical profile of Cora Contreras de Rodríguez
No full textDOI: https://doi.org/10.53766/EHI/2024.11.01.08En la presente semblanza se destaca, por parte de la autora, la amistad, admiración y el respeto hacia la vida profesional de una de las enfermeras más sobresaliente e ilustre a nivel nacional, la Licenciada Cora Contreras de Rodríguez.Recibido: 10/9/2023 Aceptado: 27/10/23Publicado en línea: 30/05/2024In this profile, the author highlights the friendship, admiration and respect for the professional life of one of the most outstanding nurses at the national level, Cora Contreras de Rodríguez
Research fronts in library and information science in Spain
No full textPublications and author cocitations in library and information science in Spain during the period from 1985 to 1994 were analyzed as a measure of the structure, specificity and composition of research fronts in this country. A cocitation matrix developed from an ad hoc database was subjected to cluster analysis, multidimensional scaling and principal components analysis, The resulting cocitation maps identified specific areas of r~search and their knowledge bases. We inferred the degree of consolidation of the discipline of library and information science, and of the subdisciplines informetrics, librarianship and university affiliation, from the research activities revealed. In this respect, the conclusions from the study show the existence of several research fronts in Spanish literature the contents of which are in most cases difficult to compare with those in other countries. A lesser degree of maturity of research in this field is shown
Cabeza
No full textThe author, Daniel Alejandro Álvarez Contreras, provides a narrative perspective on the relationship between our head and our thoughts. 
Cabeza
No full textThe author, Daniel Alejandro Álvarez Contreras, provides a narrative perspective on the relationship between our head and our thoughts. 
A Connectionist and Multivariate Approach to Science Maps: Som, Clustering and Mds Applied to Library & Information Science Research.
No full textThe visualization of scientific field structures is a classic of scientometric studies. This paper presents a domain analysis of the library and information science discipline based on author co-citation analysis (ACA) and journal cocitation analysis (JCA). The techniques used for map construction are the self-organizing map (SOM) neural
algorithm, Ward’s clustering method and multidimensional
scaling (MDS). The results of this study are compared with
similar research developed by Howard White and Katherine
McCain [1]. The methodologies used allow us to confirm that
the subject domains identified in this paper are, as well,
present in our study for the corresponding period. The appearance of studies pertaining to library science reveals
the relationship of this realm with information science.
Especially significant is the presence of the management on the journal maps. From a methodological standpoint, meanwhile, we would agree with those authors who consider
MDS, the SOM and clustering as complementary methods
that provide representations of the same reality from different analytical points of view. Even so, the MDS representation is the one offering greater possibilities for the structural representation of the clusters in a set of variables
A connectionist and multivariate approach to science maps: the SOM, clustering and MDS applied to library and information science research
No full textThe visualization of scientific field structures is a classic of scientometric studies. This paper presents a domain analysis of the library and information science discipline based on author co-citation analysis (ACA) and journal cocitation analysis (JCA). The techniques used for map construction are the self-organizing map (SOM) neural algorithm, Ward’s clustering method and multidimensional scaling (MDS). The results of this study are compared with similar research developed by Howard White and Katherine McCain [1]. The methodologies used allow us to confirm that the subject domains identified in this paper are, as well,
present in our study for the corresponding period. The appearance of studies pertaining to library science reveals the relationship of this realm with information science. Especially significant is the presence of the management on the journal maps. From a methodological standpoint, meanwhile, we would agree with those authors who consider MDS, the SOM and clustering as complementary methods that provide representations of the same reality from different analytical points of view. Even so, the MDS representation is the one offering greater possibilities for the structural representation of the clusters in a set of variables
Isomaltulose: The Next Sweetener, A Quick Review
No full textThe social behavior of new generations consume highly processed foods and high levels of fat and sugar, such as glucose or high-fructose syrup (corn syrup), commonly used in the manufacture of hard candies, have generated a growing increase in cases of diabetes and nutrition problems. In this context, we present the isomaltulose as a substitute potential for sweeteners, due to its property of maintaining stable glycemic levels, dosing energy to the organism in a long-term way, promoting the fat oxidation and it is a noncariogenic food. There is just one company that distributes isomaltulose in an industry level, under the name of Palatinose™ (Beneo-Palatinti, Sudzücker Group, Mannheim, Germany) and already incorporated isomaltulose products in the food processing as beverage, bakery, energy drinks, dry-powder drinks, chewing gum, hard candies, and others. But it is necessary to produce quantities of isomaltulose that will supply the demand of the food industry. The latest advances in biotechnology provide tools to efficiently produce 278any product that may result from the metabolism of micro-organisms. But first, it is important to know the global aspects of the enzyme, the reaction that catalyzes and its products to determine their chemical and biological parameters. This work aims to make a quick review of all these aspects that allow us to understand and start the biotechnological path for the design and development of future foods based on isomaltulose.Fil: Bracho Oliveros, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Ramírez Gutiérrez, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Contreras Esquivel, Juan Carlos. Universidad Autónoma de Coahuila; MéxicoFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin
Vaccination directed against the human endogenous retrovirus-K envelope protein inhibits tumor growth in a murine model system
No full textHuman endogenous retrovirus (HERV) genomes are chromosomally integrated in all cells of an individual. They are normally transcriptionally silenced and transmitted only vertically. Enhanced expression of HERV-K accompanied by the emergence of anti-HERV-K-directed immune responses has been observed in tumor patients and HIV-infected individuals. As HERV-K is usually not expressed and immunological tolerance development is unlikely, it is an appropriate target for the development of immunotherapies. We generated a recombinant vaccinia virus (MVA-HKenv) expressing the HERV-K envelope glycoprotein (ENV), based on the modified vaccinia virus Ankara (MVA), and established an animal model to test its vaccination efficacy. Murine renal carcinoma cells (Renca) were genetically altered to express E. coli beta-galactosidase (RLZ cells) or the HERV-K ENV gene (RLZ-HKenv cells). Intravenous injection of RLZ-HKenv cells into syngenic BALB/c mice led to the formation of pulmonary metastases, which were detectable by X-gal staining. A single vaccination of tumor-bearing mice with MVA-HKenv drastically reduced the number of pulmonary RLZ-HKenv tumor nodules compared to vaccination with wild-type MVA. Prophylactic vaccination of mice with MVA-HKenv precluded the formation of RLZ-HKenv tumor nodules, whereas wild-type MVA-vaccinated animals succumbed to metastasis. Protection from tumor formation correlated with enhanced HERV-K ENV-specific killing activity of splenocytes. These data demonstrate for the first time that HERV-K ENV is a useful target for vaccine development and might offer new treatment opportunities for diverse types of cancer
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