11 research outputs found
Genetic diversity and population biology of a global collection of phytopathogenic Verticillium dahliae
No full textRhizoctonia fragariae causes black root rot on strawberry seedlings in Turkey
No full textSamples of strawberry transplants were randomly collected from 47 growers in Aydın province of Turkey. A total of 10 Rhizoctonia isolates were identified by morphological characterization, analysis of rDNA-ITS sequences. Pathogenicity tests were carried on strawberry seedlings and detached stolon. Ten pathogenic binucleate Rhizoctonia isolates were defined as AG-A, AG-G and AG-K on the basis of ITS region sequence analysis. To our knowledge, this is the first report of the presence of Rhizoctonia fragariae in strawberry transplants in Turkey. © 2018, Australasian Plant Pathology Society Inc.110R009Acknowledgments This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) under the project no 110R009
Occurrence of Fusarium wilt caused by Fusarium oxysporum on strawberry transplants in Aydin Province in Turkey
No full textWOS: 000375927300001Samples of strawberry transplants were randomly collected from 47 growers in Aydin province of Turkey. A total of 26 Fusarium oxysporum isolates were identified by morphological characterization, sequence analysis of factor-1 alpha gene, and pathogenicity tests on strawberry, cucumber and tomato seedlings. To our knowledge, this is the first report of the presence of F. oxysporum in strawberry transplants in Turkey.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110R009]This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) under the project no 110R009
A simple and quick way for diagnosis of erwinia amylova
No full textSince it was first detected in 1985, fireblight, caused by Erwinia amylovora, has been a disease of major importance in pome fruit growing regions of Turkey. Monoclonal antibodies (MAM iF 10) specific to E. amylovora were produced to identify and detect epiphytic and endophytic E. amylovora in pome fruit tissues by sandwich enzyme linked immunosorbent assay (ELISA). MAM1F1O was purified and conjugated to both alkaline phosphatase (AP) and horseradish peroxidase (HRPO). These two conjugates reacted positively in sandwich ELISA against pure cultures of 58 E. amylovora isolates originating from different hosts and geographical areas in Turkey. MAM1FIO was also tested against 17 unidentified epiphytic bacteria from apple, quince and pear trees collected from different provinces in Turkey
Occurrence of fusarium wilt caused by Fusarium oxysporum on strawberry transplants in Aydın province in Turkey
No full textSamples of strawberry transplants were randomly collected from 47 growers in Aydın province of Turkey. A total of 26 Fusarium oxysporum isolates were identified by morphological characterization, sequence analysis of factor-1 alpha gene, and pathogenicity tests on strawberry, cucumber and tomato seedlings. To our knowledge, this is the first report of the presence of F. oxysporum in strawberry transplants in Turkey. © Australasian Plant Pathology Society Inc. 2016
Development of a double monoclonal antibody sandwich ELISA test for Verticillium dahliae Kleb.
No full textVerticillium dahliae causes wilt diseases in a wide range of horticultural and field crops in many parts of Turkey. In the Aegean region it has been a serious problem in cotton and vegetables for many years. More recently, it has become a major problem for olive growers in relatively newly established orchards. The fact that the only possible control methods of Verticillium wilt disease is the use of healthy and pathogen free propagating material has directed us to produce monoclonal antibodies against V. dahliae in order to apply rapid, sensitive and reliable serological detection methods. Verticillium spp. isolates were obtained from olive plantations, as well as from cotton and tomato fields in the Aegean and Marmara Regions. All suspected isolates were obtained as V. dahliae after determining microscopic morphological characteristics and pathogenicity tests on cotton seedlings. Immunizing antigens were prepared by three different methods including surface washing system, czapek dox agar and gel filtration methods. BALB/c mice were immunized with each antigenic form. Lymph node, spleen and bone marrow cells were used as sources of B-lymphocytes and 8D2 (IgM) and 7D6 (IgG1) were obtained from the spleen and lymph node fusion. The monoclonal antibodies were purified and immunoglobulin types were identified. 8D2 monoclonal antibody gave positive reaction with the V. dahliae isolates from olive, cotton, tomato and watermelon; however, it didn't give any cross reactivity with other epiphytic fungi. 7D6 antibody displayed cross-reactions with a few fungi. The monoclonal antibody ( 8D2) was conjugated with horseradish peroxidase (HRP). These monoclonal antibodies were characterized for use in the development of diagnostic kits based on double-monoclonal antibody sandwich ELISA test system for detecting V. dahliae in Turkish isolates. In this test, the first antibody was used as capture antibody and the second one was used for detection of antigens
First Report of Bacterial Blight Caused by <i>Pseudomonas syringae</i> pv. <i>pisi</i> on Pea in Turkey
No full text In April of 2009, leaf blight symptoms were observed on field peas (Pisum sativum L.) grown in Söke, Torbali, and Ödemis counties in the Aegean Region of Turkey. Field inspections revealed disease incidence as high as 45% and the disease was found in 13 commercial fields. Initial symptoms consisted of small, dark green, water-soaked lesions on leaves, stipules, and stems near ground level. Lesions often enlarged and coalesced and turned chocolate brown with a water-soaked margin. Stem infections usually coalesced and girdled the stem spreading upward to stipules and leaflets forming a fan-like lesion on the stipule. A fluorescent, gram-negative bacterium was consistently isolated from diseased tissues onto King's B medium. Twelve strains (five from cv. Early Sweet, three from cv. Geneva, two from cv. Bolero, and two from cv. Carina) from thirteen pea fields were obtained. All strains metabolized glucose oxidatively, and their reactions in LOPAT tests were +, —, —, —, +, and thus classified as belonging to Pseudomonas syringae LOPAT group Ia (1). The 12 strains utilized homoserine, inositol, sorbitol, sucrose, mannitol, and mannose but did not utilize erythritol, trehalose, and L-tartarate. All showed ice nucleation activity but variable results were obtained for gelatin liquefaction and esculin hydrolysis. Identification of P. syringae pv. pisi was confirmed by sequencing the 16S rDNA with primers Univ-1390R (3) and 27F (2). Sequences of the three local strains (Bz2, Bz4, and Bz8) were 100% identical to a type culture strain. The nucleotide sequence of strain Bz4 was submitted to GenBank (Accession No. GU332546). Pathogenicity tests were performed on greenhouse-grown 2-week-old pea plants cv. Geneva as three replicates in 12-cm pots containing a steamed sand/peat/soil mixture. Plants were stab inoculated by puncturing the main stem at its junction with the stipules at the second node from the apical end with a 26-gauge needle through a 5-μl drop of 108 CFU/ml bacterial suspensions. Control plants were inoculated with sterile water. After 10 days of incubation in a growth chamber at 24 ± 1°C with a 14-h photoperiod, stems inoculated with pea isolates resulted in water-soaked tissue spreading from the site of inoculation along the veins on stipules and leaflets that were identical to symptoms seen in the field. Control plants remained symptomless. Isolates recovered from the symptomatic stems showed the same morphological and biochemical features of the original isolates. All physiological and biochemical tests as well as the pathogenicity assay were performed at least twice and the type strain of P. syringae pv. pisi (NCPPB 2585) was used as reference. On the basis of the physiological, biochemical, genetic, and pathological characteristics, all strains were identified as P. syringae pv. pisi. To our knowledge, this is the first report of P. syringae pv. pisi causing bacterial blight on pea in Turkey. Turkey currently produces approximately 93.000 t of peas annually and three-quarters of that is produced in Western Anatolia. The new disease may represent a limiting factor for future production. References: (1) R. A. Lelliott et al. J. Appl. Bacteriol. 29:470, 1966. (2) W. G. Weisburg et al. J. Bacteriol. 173:697, 1991. (3) D. Zeng et al. Appl. Environ. Microbiol. 62:4504, 1996. </jats:p
Globally invading populations of the fungal plant pathogen Verticillium dahliae are dominated by multiple divergent lineages
No full textThe spread of aggressive fungal pathogens into previously non-endemic regions is a major threat to plant health and food security. Analyses of the spatial and genetic structure of plant pathogens offer valuable insights into their origin, dispersal mechanisms and evolution, and have been useful to develop successful disease management strategies. Here, we elucidated the genetic diversity, population structure and demographic history of worldwide invasion of the ascomycete Verticillium dahliae, a soil-borne pathogen, using a global collection of 1100 isolates from multiple plant hosts and countries. Seven well-differentiated genetic clusters were revealed through discriminant analysis of principal components (DAPC), but no strong associations between these clusters and host/geographic origin of isolates were found. Analyses of clonal evolutionary relationships among multilocus genotypes with the eBURST algorithm and analyses of genetic distances revealed that genetic clusters represented several ancient evolutionary lineages with broad geographic distribution and wide host range. Comparison of different scenarios of demographic history using approximate Bayesian computations revealed the branching order among the different genetic clusters and lineages. The different lineages may represent incipient species, and this raises questions with respect to their evolutionary origin and the factors allowing their maintenance in the same areas and same hosts without evidence of admixture between them. Based on the above findings and the biology of V. dahliae, we conclude that anthropogenic movement has played an important role in spreading V. dahliae lineages. Our findings have implications for the development of management strategies such as quarantine measures and crop resistance breeding
Effects of Juglone on Growth of Muskmelon Seedlings with Respect to Physiological and Anatomical Parameters
No full textGrowth parameters (seedling elongation, fresh and dry masses) and protein content of Cucumis melo were enhanced by juglone (allelochemical occuring in the walnut family) treatment in pregerminative stage but decreased in postgerminative treatment. Catecholase and tyrosinase activities were also increased in both treatments. Xylem vessel radius of stem was enhanced significantly by the pregerminative treatment, whereas it decreased slightly by the postgerminative treatment. However, bundle radius of stem was enhanced by both treatments of juglone. Stomata length and number were not changed significantly. Xylem vessel radius of the stem was affected by juglone more than the other parameters
SARS-CoV-2 infection in pregnancy: A systematic review and meta-analysis of clinical features and pregnancy outcomes.
No full textBackground: Perform a systematic review and meta-analysis of SARS-CoV-2 infection and pregnancy. Methods: Databases (Medline, Embase, Clinicaltrials.gov, Cochrane Library) were searched electronically on 6th April and updated regularly until 8th June 2020. Reports of pregnant women with reverse transcription PCR (RT-PCR) confirmed COVID-19 were included. Meta-analytical proportion summaries and meta-regression analyses for key clinical outcomes are provided. Findings: 86 studies were included, 17 studies (2567 pregnancies) in the quantitative synthesis; other small case series and case reports were used to extract rarely-reported events and outcome. Most women (73.9%) were in the third trimester; 52.4% have delivered, half by caesarean section (48.3%). The proportion of Black, Asian or minority ethnic group membership (50.8%); obesity (38.2%), and chronic co-morbidities (32.5%) were high. The most commonly reported clinical symptoms were fever (63.3%), cough (71.4%) and dyspnoea (34.4%). The commonest laboratory abnormalities were raised CRP or procalcitonin (54.0%), lymphopenia (34.2%) and elevated transaminases (16.0%). Preterm birth before 37 weeks' gestation was common (21.8%), usually medically-indicated (18.4%). Maternal intensive care unit admission was required in 7.0%, with intubation in 3.4%. Maternal mortality was uncommon (~1%). Maternal intensive care admission was higher in cohorts with higher rates of co-morbidities (beta=0.007, p<0.05) and maternal age over 35 years (beta=0.007, p<0.01). Maternal mortality was higher in cohorts with higher rates of antiviral drug use (beta=0.03, p<0.001), likely due to residual confounding. Neonatal nasopharyngeal swab RT-PCR was positive in 1.4%. Interpretation: The risk of iatrogenic preterm birth and caesarean delivery was increased. The available evidence is reassuring, suggesting that maternal morbidity is similar to that of women of reproductive age. Vertical transmission of the virus probably occurs, albeit in a small proportion of cases. Funding: N/A
