6,106 research outputs found
Prdx1 deficiency in mice promotes tissue specific loss of heterozygosity mediated by deficiency in DNA repair and increased oxidative stress
No full textLoss of the H2O2 scavenger protein encoded by the peroxiredoxin 1 (Prdx1) gene in mice leads to elevated intracellular amounts of reactive oxygen species (ROS) and promotes tumorigenesis in several different tissues. Loss of heterozygosity (LOH) mutations can initiate tumorigenesis through loss of tumor suppressor gene function in somatic cells that carry only one functional allele. A connection between the severity of oxidative stress and the frequency of LOH mutations has not been previously established in vivo. Therefore, in this study, we characterized in vivo LOH in ear fibroblasts and splenic T cells of 3-4 month old Prdx1-deficient mice. We found that loss of Prdx1 significantly elevates ROS levels in T cells and fibroblasts, and that basal levels of ROS are higher in fibroblasts than in T cells, probably due to a less robust Prdx1 peroxidase activity in the former. Using Aprt as a LOH reporter, we observed an elevation in LOH mutation frequency in fibroblasts, but not in T cells, of Prdx1-/- mice compared to Prdx1+/+ mice. The majority of the LOH mutations in both cell types were derived from mitotic recombination (MR) events. Interestingly, Mlh1, which is known to suppress MR between divergent sequences, was found to be significantly down-regulated in fibroblasts, but not in T cells, of Prdx1-/- mice. Additionally, there was no compensatory increase in expression of base excision repair (BER) genes in Prdx1-/- fibroblasts. Since these cells had higher amounts of ROS, it suggests that BER activity may be insufficient to manage the increased oxidative DNA damage load. This could lead to increased formation of SSBs and DSBs, which could require repair by recombination. A combination of increased amounts of ROS, down-regulation of Mlh1 and inefficient BER may have contributed to the elevation of MR in fibroblasts of Prdx1-/- mice. Additionally, my findings suggest that helix-distorting lesions may also be increased in Prdx1-/- ear fibroblasts and T cells. Transcription analyses of genes in different DNA repair pathways in the whole spleen tissue with Prdx1 deficiency revealed significant differences in transcription compared to T cells. I conclude that mechanisms through which Prdx1 deficiency promotes tumorigenesis in specific tissues may be distinct.Ph. D.Includes bibliographical referencesIncludes vitaby Vamsi K. Ran
A panoramic view of motherhood by juxtaposing the select trilogies of Flora Nwapa and Perumal Murugan
No full text<p>This article by Vimala Johnshi Rani compares the portrayal of motherhood in the works of African writer Flora Nwapa and Indian writer Perumal Murugan. By examining trilogies by each author, Rani explores how women in post-colonial South Africa and India navigate societal expectations and personal desires.</p>
A new species of Pseudosesarma Serène & Soh, 1970 (Crustacea: Brachyura: Sesarmidae) from Cochin in southwestern India
No full textNg, Peter K. L., Rani, Varghese, Nandan, S. Bijoy (2017): A new species of Pseudosesarma Serène & Soh, 1970 (Crustacea: Brachyura: Sesarmidae) from Cochin in southwestern India. Zootaxa 4311 (2): 263-270, DOI: https://doi.org/10.11646/zootaxa.4311.2.
Review: Chinese Whispers, immersive installation based performance directed by Rani Pramesti
No full textReview:
Chinese Whispers, immersive installation based performance directed by Rani Pramest
The Brylinski beta function of a coaxial layer
No full textIn [Pooja Rani and M. K. Vemuri, The Brylinski beta function of a double layer, Differential Geom. Appl. \textbf{92}(2024)], an analogue of Brylinski\u27s knot beta function was defined for a compactly supported (Schwartz) distribution on Euclidean space. Here we consider the Brylinski beta function of the distribution defined by a coaxial layer on a submanifold of Euclidean space. We prove that it has an analytic continuation to the whole complex plane as a meromorphic function with only simple poles, and in the case of a coaxial layer on a space curves, we compute some of the residues in terms of the curvature and torsion
Introduction: Why is philosophy relevant for clinical practice?
No full textThis book is intended as an intellectual resource for clinicians and healthcare professionals who are interested in digging deeper into the philosophical foundations of their daily practice. It is a tool for understanding some of the philosophical motivations and rationality behind the way medicine and healthcare are studied, evaluated and practiced. In particular, this book illustrates the impact that our thinking about causality, both philosophically and conceptually, has on the clinical encounter. The aim is to engage and empower healthcare professionals to take part in changing and defining the premises for their own practice. After all, if clinical decisions should be based on evidence, this ought to be evidence that is relevant and well-suited for the clinic. The book has two parts, Philosophical Framework and Application to the Clinic. The first part is written from the philosophical perspective and presents a singular overall framework. The second part is written primarily by clinicians who address some implications of the philosophical framework for different aspects of their own practice.Ethics & Philosophy of Technolog
Conclusion: Causehealth recommendations for making causal evidence clinically relevant and informed
No full textFrom the philosophical perspective presented in the first part of this book, together with the clinical application of this framework in the second part, it follows that we must change the way we approach causal evidence of health and illness conceptually, methodologically and practically. This has some practical consequences for the clinical encounter, as discussed throughout the book. We here offer some specific CauseHealth recommendations for making causal evidence more clinically relevant and better informed by the clinical encounter. The recommendations follow from an overall consideration of the previous chapters, both the philosophical framework and its clinical implications.Ethics & Philosophy of Technolog
Radio Heronian Mean k-Graceful Labeling on Degree Splitting of Graphs
No full textA mapping g:V\left(G\right)\rightarrow{k,k+1,\ldots,k+N-1} is a radio heronian mean k-labeling such that if for any two distinct vertices s and t of G, d\left(s,t\right)+\left\lceil\frac{g\left(s\right)+g\left(t\right)+\sqrt{g\left(s\right)g\left(t\right)}}{3}\right\rceil\geq1+D,for every s,t\in\ V(G), where D is the diameter of G. The radio heronian mean k-number of g, {rrhmn}_k(g), is the maximum number assigned to any vertex of G. The radio heronian mean number of G, {rhmn}_k(g), is the minimum value of {rhmn}_k(g) taken overall radio heronian mean labelings g of G. If {rhmn}_k(g)=\left|V\left(G\right)\right|+k-1, we call such graphs as radio heronian mean k-graceful graphs. In this paper, we investigate the radio heronian mean k-graceful labeling on degree splitting of graphs such as comb graph P_n\bigodot K_1, rooted tree graph {RT}_{n,n} hurdle graph {Hd}_n and twig graph\ {TW}_n.A mapping is a radio heronian mean k-labeling such that if for any two distinct vertices and of , ,for every V(G), where is the diameter of . The radio heronian mean k-number of g, , is the maximum number assigned to any vertex of . The radio heronian mean number of , , is the minimum value of taken overall radio heronian mean labelings of . If , we call such graphs as radio heronian mean k-graceful graphs. In this paper, we investigate the radio heronian mean k-graceful labeling on degree splitting of graphs such as comb graph , rooted tree graph hurdle graph and twig graph
Microorganisms. A potential source of bioactive molecules for antioxidant applications
No full textOxidative stress originates from an elevated intracellular level of free oxygen radicals that cause lipid peroxidation, protein denaturation, DNA hydroxylation, and apoptosis, ultimately impairing cell viability. Antioxidants scavenge free radicals and reduce oxidative stress, which further helps to prevent cellular damage. Medicinal plants, fruits, and spices are the primary sources of antioxidants from time immemorial. In contrast to plants, microorganisms can be used as a source of antioxidants with the advantage of fast growth under controlled conditions. Further, microbe-based antioxidants are nontoxic, noncarcinogenic, and biodegradable as compared to synthetic antioxidants. The present review aims to summarize the current state of the research on the antioxidant activity of microorganisms including actinomycetes, bacteria, fungi, protozoa, microalgae, and yeast, which produce a variety of antioxidant compounds, i.e., carotenoids, polyphenols, vitamins, and sterol, etc. Special emphasis is given to the mechanisms and signaling pathways followed by antioxidants to scavenge Reactive Oxygen Species (ROS), especially for those antioxidant compounds that have been scarcely investigated so far
Nuevos datos sobre el verdadero autor de «La Rani-ratiguerra» (1790), en la correspondencia del conde de Lumiares
No full textRESUMENEn la correspondencia que, entre 1773 y 1778, mantuvo Antonio Valcárcel Pío de Saboya, conde de Lumiares, con el ilustrado Gregorio Mayans y con el anticuario gaditano Antonio Mosti, se contienen ciertas manifestaciones relativas a una poesía manuscrita inédita que Valcárcel tenía intención de publicar de forma anónima pese a saber que su autor era el jesuita Martín Marín. El texto de dicho poema, conservado hoy en la Biblioteca Estense Universitaria de Módena, evidencia que se trata de la misma obra que en 1790 apareció publicada anónimamente en Valencia con el nombre de «La Raniratiguerra», y cuya autoría se atribuyó, bien entrado ya el siglo XIX, al dramaturgo José March Borrás; una atribución que ha perdurado hasta hoy y que debe ser objeto de revisión a la luz de los testimonios aportados por Valcárcel en su correspondencia.PALABRAS CLAVEConde de Lumiares, correspondencia, Batracomiomaquia, Rani-ratiguerra, José March Borrás, Martín Marín. TITLENew data about the true author of «La Rani-ratiguerra» (1790), in the correspondence of the count of Lumiares.ABSTRACTIn the correspondence maintained between 1773 and 1778 by Antonio Valcárcel Pío de Saboya, count of Lumiares, with the illustrated Gregorio Mayans and the antiquarian Antonio Mosti, there are certain manifestations related to an unpublished manuscript poetry that Valcárcel intended to publish anonymously despite knowing that its author was the jesuit Martín Marín. The text of this poem, preserved today in the Estense University Library of Modena, shows that it is the same poem that in 1790 was published anonymously in Valencia under the name of «La Rani-ratiguerra», whose authorship was attributed in the nineteenth century to the playwright José March Borrás; an attribution that has lasted until today and that must be object of revision in the light of the testimonies contributed by Valcárcel in his correspondence.KEY WORDSCount of Lumiares; correspondence, Batracomiomaquia, Rani-ratiguerra; José March Borrás, Martín Marín
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