28 research outputs found
Nuclear organization of mouse Hox cluster paralogs during mouse embryonic stem cell differentiation to neural stem cell:
No full textIn this project we use Hox genes as a genetic tool to understand how nuclear architecture regulates cell differentiation during embryonic development. Hox genes come under the category of homeobox genes, a highly evolutionarily conserved group of genes with an important role during embryogenesis. Hox genes are located on 4 distinct chromosomes, in cluster paralogs (HOX A, B, C, D). Each individual cluster contains up to 13 homologous genes and corresponding genes on different clusters (e.g., HoxA13, HoxD13) exhibit varying degrees of functional redundancy. The position of a gene in the cluster is related to its spatiotemporal pattern of expression along the anterior-posterior axis of the embryo. The coordination of the spatiotemporal expression of equivalent paralog group genes on different clusters/chromosomes is coordinated is still not known. Our primary hypothesis is that nuclear architecture defines a regulatory framework of Hox cluster loci in the nucleus when the Hox cluster transcription is activated and maintained. We did a comparative analysis on the Hox cluster nuclear architecture in mouse embryonic stem cells (ESCs) and fibroblast growth factors (FGF) - induced differentiation to neural stem cells (NSC). We show for the first time that Hox gene expression is induced by FGF treatment in vitro simultaneously in the four Hox cluster. Using three-dimensional confocal fluorescence microscopy, FISH and computational techniques, we mapped the position of Hox gene cluster paralogs in individual nuclei of both cell types. We did not observe nuclear colocalization of Hox heterologous cluster in NSC. However, we observe that heterologous clusters tend to occupy similar nuclear domains in NSC, which may favor undetected long-range gene interactions. Nevertheless, our results indicate that Hox gene cluster nuclear three-dimensional organization is neither random nor correlated to the changes in nuclear volume and shape that parallel cell differentiation.M.S.Includes bibliographical references (p. 53-55)by Priya Panicke
Ethical task tracking of operators in agile manufacturing
No full textDiversey BV, a major player of professional hygiene product manufacturing, is facing challenges with agile manufacturing of hygienic products with changeover process consuming most of production time. They are collaborating with the EU-Horizon 2020 COALA project to develop a cognitive intelligent assistant for the production line. They expect to standardize activities in the production line to reduce the gap in activity performance between experienced and novice operators. In order to set up the cognitive assistant, an operator location tracking system was needed to identify issue hotspots and sequence of activities in the production line. In this project, a suitable motion capture system was explored and deployed at Diversey Enschede 5L/ 10L production line. A literature study was performed to compare the state of the art motion capture and motion analysis methods. From the literature study results, the project decided to deploy a markerless motion capture method using Zed 2 camera.The data collection method was tested at Enschede with Zed 2 camera which has in-built object tracking algorithms. The project applied an ethical approach to operator tracking, giving due respect to operators’ privacy concerns and anonymity. The Value Sensitive Design method was applied in this project to identify the stakeholders, their values, and the project’s future speculation. The data collection, storage and upload to cloud server was conducted using indefinitely running Python codes. The tracking was anonymized by allocating random identification numbers to denote objects and thereby, no personal data that can identify the operator were being stored. The data was captured and stored in spreadsheet format and processed using Python. The project concludes with the implementation of Z-Dash, an interactive tool that visualizes the data in various meaningful representations. Z-Dash offers graphs such as the Spaghetti chart for visualizing operator location and movements, Heat map of operator location concentration and Pareto chart that visualizes time and frequency of visited stations. The tool was evaluated with participants from Diversey to estimate the usability, interactivity and effectiveness for process improvement. The project proposes this tool for identifying the sequence of operator activities during events like changeover or stoppages, identifying issue hotspots and comparing best practices for similar events.Integrated Product Desig
Fine-scale spatial and temporal acoustic occurrence of island-associated odontocetes near a mid-oceanic atoll in the northern Indian Ocean
No full text© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Panicker, D., Baumgartner, M. F., & Stafford, K. M. Fine-scale spatial and temporal acoustic occurrence of island-associated odontocetes near a mid-oceanic atoll in the northern Indian Ocean. Marine Ecology Progress Series, 683, (2022): 195–208, https://doi.org/10.3354/meps13947.Temporal patterns of oceanic predators can provide valuable information on both lunar and diel influences not just on the distributions of these predators, but also on prey patches that are often difficult to study. Mid-oceanic island chains in the northern Indian Ocean have high odontocete occurrence, but the ecology of these animals is not well characterized. We investigated fine-scale spatial and temporal patterns of island-associated odontocetes using passive acoustic monitoring from January 2019 to January 2020 around Kavaratti Island, Lakshadweep, India. Based on opportunistic recordings in the presence of odontocetes, the majority of the detected whistles were likely made by spinner dolphins Stenella longirostris. We identified a resident population whose whistle occurrence was significantly influenced by month, site, and diel and lunar cycles. More acoustic detections were made in the northeast monsoon month of November and fewer during pre-monsoon and southwest monsoon periods. Distinct day-night differences along with fine-scale temporal variability were also observed, suggesting that delphinids use nearshore waters as a daytime resting habitat. Odontocete detections were highest during the new moon period and lowest during the first quarter phase. Detection rates were higher on the south side of the island. Our study shows that solar and lunar cycles modulate odontocete vocal occurrence, presumably through influences on their prey. Similarities of odontocete occurrence around Lakshadweep to other mid-oceanic island chains suggests that an island-associated micronekton community may exist around Lakshadweep that may also be important to other pelagic species targeted by local fisheries.Funding was provided by the Office of Naval Research Marine Mammal Biology Program, USA, under grant N000141812795.
We thank Ajith Kumar, the National Centre for Biological Sciences and Idrees Babu for in-country support
Status of Fermented Dairy Products in India Safety and Spoilage Aspects
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Microbial quality of Dahi and Challenge Studies
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Serum Immune Profiling for Early Detection of Cervical Disease
No full textabstract: Background: The most recent (2012) worldwide estimates from International Agency for Research on Cancer indicate that approximately 528,000 new cases and 270,000 deaths per year are attributed to cervical cancer worldwide. The disease is preventable with HPV vaccination and with early detection and treatment of pre-invasive cervical intraepithelial neoplasia, CIN. Antibodies (Abs) to HPV proteins are under investigation as potential biomarkers for early detection.
Methods: To detect circulating HPV-specific IgG Abs, we developed programmable protein arrays (NAPPA) that display the proteomes of two low-risk HPV types (HPV6 and 11) and ten oncogenic high-risk HPV types (HPV16, 18, 31, 33, 35, 39, 45, 51, 52 and 58). Arrays were probed with sera from women with CIN 0/I (n=78), CIN II/III (n=84), or invasive cervical cancer (ICC, n=83).
Results: Abs to any early (E) HPV protein were detected less frequently in women with CIN 0/I (23.7%) than women with CIN II/III (39.0%) and ICC (46.1%, p<0.04). Of the E Abs, anti-E7 Abs were the most frequently detected (6.6%, 19.5%, and 30.3%, respectively). The least frequently detected Abs were E1 and E2-Abs in CIN 0/I (1.3%) and E1-Abs in CIN II/III (1.2%) and ICC (7.9%). HPV16-specific Abs correlated with HPV16 DNA detected in the cervix in 0% of CIN 0/I, 21.2% of CIN II/III, and 45.5% of ICC. A significant number (29 - 73%) of E4, E7, L1, and L2 Abs had cross-reactivity between HPV types.
Conclusion: HPV protein arrays provide a valuable high-throughput tool for measuring the breadth, specificity, and heterogeneity of the serologic response to HPV in cervical disease
A Cognitive Assistant for Operators: AI-Powered Knowledge Sharing on Complex Systems
No full textOperating a complex and dynamic system, such as an agile manufacturing line, is a knowledge-intensive task. It imposes a steep learning curve on novice operators and prompts experienced operators to continuously discover new knowledge, share it, and retain it. In practice, training novices is resource-intensive, and the knowledge discovered by experts is not shared effectively. To tackle these challenges, we developed an AI-powered pervasive system that provides cognitive augmentation to users of complex systems. We present an AI cognitive assistant that provides on-the-job training to novices while acquiring and sharing (tacit) knowledge from experts. Cognitive support is provided as dialectic recommendations for standard work instructions, decision-making, training material, and knowledge acquisition. These recommendations are adjusted to the user and context to minimize interruption and maximize relevance. In this article, we describe how we implemented the cognitive assistant, how it interacts with users, its usage scenarios, and the challenges and opportunities.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Internet of Thing
Neutrophilic eccrine hidradenitis secondary to pegfilgrastim in a patient with synovial sarcoma
No full textA grant from the One-University Open Access Fund at the University of Kansas was used to defray the author's publication fees in this Open Access journal. The Open Access Fund, administered by librarians from the KU, KU Law, and KUMC libraries, is made possible by contributions from the offices of KU Provost, KU Vice Chancellor for Research & Graduate Studies, and KUMC Vice Chancellor for Research. For more information about the Open Access Fund, please see http://library.kumc.edu/authors-fund.xml.Key Clinical Message
Here, we report a case of neutrophilic eccrine hidradenitis (NEH) in a teenage male
with synovial sarcoma associated with extracutaneous manifestations including myositis and splenomegaly secondary to pegfilgrastim. To the best of our knowledge,
NEH has not been previously reported to occur in association with extracutaneous
manifestations
Degraded Gods on parade : Reading Temple Festivals in Early Malayalam Literature
No full textThis article concerns a representation of a temple festival in Tiruniḻalmāla, a major literary work in early Malayalam. The work in question focuses on a high-order Brahmanic temple of Āṟanmuḷa in the moment of a grave ritual and operational crisis. It emphasizes the restorative role of rather lowly rites of exorcism and purification including elements of dance, song, and drama. The article pays close attention to the structure of its textual matter and the literary strategies deployed by its author in meeting the performative exigencies of the genre. The festival itself seems today to have been long forgotten in the place of its origin. The article attempts to understand the socioreligious functions of the festival by exploring the inner logic of its literary representation, drawing parallels to other cultural institutions of medieval Kerala and showing how its afterlife in folk possession dance rituals contributed to the transmission of a text that gave the festival its literary form
