1,042 research outputs found
Documents: Andrew Atkinson Humphreys\u27 Seminole War Field Journal
No full textAndrew Atkinson Humphreys (1810-1883) was born into a prominent Philadelphia family of Quaker origin. His grandfather, Joshua Humphreys, a distinguished naval architect later known as the Father of the American Navy, served as chief naval constructor (1794-1801) and designed the first U.S. warships, including the Constitution ( Old Ironsides ) and her five sister ships
Review of \u3ci\u3eI\u27ll Be Here in the Morning: The Songwriting Legacy of Townes Van Zandt\u3c/i\u3e by Brian T. Atkinson
No full textTexas\u27s Townes Van Zandtwas a musician\u27s musician whose fame grew after his 1996 death. Brian T. Atkinson, contributor to the Austin AmericanStatesman, Texas Music, Lone Star, American Songwriter, and No Depression, has woven together a collection of interviews from Van Zandt\u27s contemporaries and friends, as well as his musical heirs-singer-songwriters who grew up too late to have known the troubled author of Pancho and Lefty, Tecumseh Valley, and Lungs but who admired his dark, poetic lyrics
TIME EVOLUTION OF SHORT-LIVED MOLECULAR SPECIES OBSERVED BY INTRACAVITY LASER ABSORPTION SPECTROSCOPY
No full textAuthor Institution: Laboratoire de Spectcometrie Physique Universite Scientifique et Medicale de Grenoble BP68 38042, Saint Martin d'Heres; Department of Chemistry, Davidson College, Davidson; Department of Chemistry, Syracuse University SyracuseWe report the feasibility of using CW intracavity laser absorption spectroscopy (ICLAS) as a probe in detecting transient species generated by pulsed laser photolysis. The method of detection uses a time-generated CW laser beam and time-resolved spectra which are delayed with respect to the photolysis pulse. Variable time delays and a constant generation time have been used to detect the time dependence of the absorbing species at times as short as a few microseconds. We applied the technique to the study of the time evolution of the formation of the free radical HCO generated by photolysis of gas-phase acetaldehyde at pressures down to tens of mtorr. We have also found that the ultimate sensitivity of the ICLAS technique is limited mainly by the mechanical stability of the cavity. M. Chenevier, M. A. Melieres, F. Stoeckel, to be published G. H. Atkinson, A. H. Laufer and M. J. Kurylo, J. Chem. Phys. 59 (1973) 350. G. H. Atkinson, T. M. Heimlich and M. W. Schuyler, J. Chem. Phys. 66 (1977 5005. A. J. Gill and G. H. Atkinson, Chem. Phys. Letters, 64 (1979) 426. R, J. Gill, W. D. Johnson and G. H. Atkinson, Chem. Phys. 58 (1981) 29
Controlling bioenergetic systems using protein design and synthetic biology.
No full textAn understanding of the mechanisms that life uses to regulate this flow of energy and how to program them at different scales is becoming of great importance for the field of synthetic biology as researches build living systems with ever increasing complexity. My thesis goals are to determine design rules for programming the function of proteins that control energy charge and electron transfer in cells. Herein, I describe my efforts in developing a computational pipeline for analyzing sequencing data from bacterial growth selections that depend on the function of adenylate kinase, a protein that controls cellular energy charge, applying this pipeline to libraries of topological mutants to uncover trends in how the energetic frustration in an allosteric domain relates to tolerance to increased local conformational entropy, developing a high-throughput growth selection for monitoring the efficiency of an electron transfer pathway in vivo, design of synthetic allosteric metalloprotein switches to control electron transfer in the cytosol of cells, and finally coupling cytosolic metabolism to a synthetic extracellular respiratory circuit that enables the transfer of intracellular electrons to surface of cells for reduction of conductive materials. These studies help enable synthetic biology strategies for the control of bioenergetics across a variety of length scales including from local energetics of protein structures to the energy charge of the cell to the energetic interface of cells and materials
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Uncovering the neuromolecular basis for hygrotaxis in Caenorhabditis elegans
No full textAll terrestrial animals must find a proper level of moisture to ensure their health and survival. However, the molecular basis for sensing humidity is unknown in most animals. We used the model nematode Caenorhabditis elegans to uncover a novel mechanism to sense humidity. We found that C. elegans displayed a strong preference, orienting to gradients as shallow as 0.03% relative humidity per mm. Cell-specific ablation and rescue experiments demonstrate that orientation to humidity in C. elegans requires the obligatory combination of distinct mechanosensitive and thermosensitive pathways. The mechanosensitive pathway requires a conserved DEG/ENaC/ASIC mechanoreceptor complex in the FLP neuron pair. Because humidity levels influence the hydration of the worm’s cuticle, our results suggest that FLP may convey humidity information by reporting the degree that subcuticular dendritic sensory branches of FLP neurons are stretched by hydration. The thermosensitive pathway requires cGMP-gated channels in the AFD neuron pair. Because humidity levels affect evaporative cooling, AFD may convey humidity information by reporting thermal flux. Thus, humidity sensation arises as a metamodality in C. elegans that requires the integration of parallel mechanosensory and thermosensory pathways. This hygrosensation strategy, first proposed by Thunberg over 100 years ago, may be conserved because the underlying pathways have cellular and molecular equivalents across a wide range of species including insects and humans. Whereas well-fed worms raised in non-crowded conditions prefer high humidity levels starved worms prefer lower humidity ranges. Our results suggest a model in which social cues from pheromone signaling, along with the worm’s feeding state, influence cyclic guanosine monophosphate (cGMP), peptide, and protein kinase C signaling to produce opposite hygrotaxis behavior.Cellular and Molecular Biolog
Global new product introduction and development in the automotive sector
No full textA thesis submitted in partial fulfilment of the
requirements of the University of Wolverhampton
for the degree of Master of PhilosophyA Global New Product Introduction and Development (GNPID) process is one of the cornerstones towards a competitive advantage in the automotive marketplace today. A fully optimised GNPID process in combination with other lean and agile manufacturing techniques and systems is guaranteed to reduce lead-time and save on cost. In the typical post-launch product life-cycle the problems faced by most manufacturing companies lies not only in accelerating and maintaining sales after the launch but in reducing the costly development time before the launch. In an effort to improve timelines and effectiveness, a number of firms within the automotive industry are experimenting with different best practices in their NPID processes. While much of the previous research has focused on NPID in a single location, little has been reported on how actual companies are addressing the problems with globalisation of NPID. The author aims to develop a set of methodologies for rapid new product introduction in a global manufacturing environment using an integrated framework of concurrent engineering tools and methods. This is to support the development of customer focused agile product and to meet customer expectations in terms of innovation and customisation, quality, competitive price, sustainable and environmentally friendly product
CR1 Knops blood group alleles are not associated with severe malaria in the Gambia
No full textThe Knops blood group antigen erythrocyte polymorphisms have been associated with reduced falciparum malaria-based in vitro rosette formation (putative malaria virulence factor). Having previously identified single-nucleotide polymorphisms (SNPs) in the human complement receptor 1 (CR1/CD35) gene underlying the Knops antithetical antigens Sl1/Sl2 and McC(a)/McC(b), we have now performed genotype comparisons to test associations between these two molecular variants and severe malaria in West African children living in the Gambia. While SNPs associated with Sl:2 and McC(b+) were equally distributed among malaria-infected children with severe malaria and control children not infected with malaria parasites, high allele frequencies for Sl 2 (0.800, 1,365/1,706) and McC(b) (0.385, 658/1706) were observed. Further, when compared to the Sl 1/McC(a) allele observed in all populations, the African Sl 2/McC(b) allele appears to have evolved as a result of positive selection (modified Nei-Gojobori test Ka-Ks/s.e.=1.77, P-valu
Music in Action: Tinkering, Testing, and Tracing Over Time
No full textArticleThis is the author accepted manuscript. The final version is available from SAGE Publications via the DOI in this record.In this article we draw on a recent, six-year ethnographic study of community music therapy and mental health to highlight strategies and techniques for documenting music’s role in processes of change. We place these strategies in dialogue with the ethnographic work on arts and crafts by Paul Atkinson. In tandem with Atkinson, we propose a ‘slow’ approach focused on micro-processes of musical/para-musical bricolage whereby things are made and transformed over time. A three-cornered strategy in support of this approach is described: (1) a focus on musical-practical tinkering, (2) a focus on the modification and contention or testing of idiocultural musical space and, (3) two specific techniques for tracing music-related change, the music therapy ‘index’ and the ‘musical event’ schema
Localization of an accessory helicase at the replisome is critical in sustaining efficient genome duplication
No full text© The Author(s) 2010. Published by Oxford University PressGenome duplication requires accessory helicases to displace proteins ahead of advancing replication forks. Escherichia coli contains three helicases, Rep, UvrD and DinG, that might promote replication of protein-bound DNA. One of these helicases, Rep, also interacts with the replicative helicase DnaB. We demonstrate that Rep is the only putative accessory helicase whose absence results in an increased chromosome duplication time. We show also that the interaction between Rep and DnaB is required for Rep to maintain rapid genome duplication. Furthermore, this Rep–DnaB interaction is critical in minimizing the need for both recombinational processing of blocked replication forks and replisome reassembly, indicating that colocalization of Rep and DnaB minimizes stalling and subsequent inactivation of replication forks. These data indicate that E. coli contains only one helicase that acts as an accessory motor at the fork in wild-type cells, that such an activity is critical for the maintenance of rapid genome duplication and that colocalization with the replisome is crucial for this function. Given that the only other characterized accessory motor, Saccharomyces cerevisiae Rrm3p, associates physically with the replisome, our demonstration of the functional importance of such an association indicates that colocalization may be a conserved feature of accessory replicative motors.Biotechnology and Biological Sciences Research Council (BB/G005915/1 and BB/E0020690 to P.M.); MRC (G0800970 to R.G.L.); Leverhulme Trust (to C.J.R.). Funding for open access charge: BBSRC
Telling interactive stories: A practice-based investigation into new media interactive storytelling
No full textThis thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Telling Interactive Stories is a practice-based thesis, which theoretically and practically probes the field of digital fictional interactive storytelling. The submission takes the form of the interactive cinema installation Crossed Lines
together with a written element of the thesis which interrogates historical, contextual, theoretical, technical and critical aspects of the field of interactive narrative using new media. Crossed Lines is an original fictional interactive AV piece, amalgamating multiform plots, a multi-screen viewing environment, an
interactive interface and an interactive story navigation form. The installation tells the stories of nine characters in a way that the viewer can constantly explore and switch between all nine forms, using a telephone keypad and handset as an interface, and can simultaneously observe all characters’ presence between the
nine remote locations. Several research methodologies are utilised to analyse and
evaluate the installation. Quantitative methodologies include the use of user tracking systems where the computational output of the installation provides measurements and timings of user choices and behaviours. Qualitative
methodologies include theoretical and visual analysis, and in depth analysis of user responses using interviews, questionnaires, video recordings and cuttingedge eye-tracking technologies
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