8 research outputs found
{'en_US': 'Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers'}
No full textGenotype and nitrogen effects on grain yield and yield-related traits of maize (Zea mays L.) hybrids
No full textSaabunud / Received 01.11.2021 ; Aktsepteeritud / Accepted 17.12.2021 ; Avaldatud veebis / Published online 18.12.2021 ; Vastutav autor / Corresponding author: Ganga Paudel [email protected] and nitrogen application are important determinants of grain yield in maize. This experiment was carried out in split-plot design with two factors (maize hybrids as the main factor and nitrogen rates as the sub factor) arranged in randomized complete block design (RCBD) with three replications. The maize hybrids including Rampur Hybrid-2, Rampur Hybrid-4 and Rampur Hybrid-6 were main plots, and four nitrogen rates of 0, 140,160, and 180 kg N ha–1 were sub-plot. Results revealed that hybrids had significant effects on the days to 50% tasselling, cob circumference, number of kernel rows per cob and grain yield. Similarly, nitrogen rates had significant effects on all parameters except on the days to 50% tasselling and silking. The highest values of cob length (18.31 cm), no of kernel rows per cob (13.22), no of kernels per cob (33.36), cob circumference (13.90 cm) was recorded from the plot fertilized with 180 kg N ha–1. Rampur Hybrid-2 produced the highest yield (6.19 t ha–1), whereas the lowest yield was found in Rampur Hybrid-6. Similarly, 180 kg N ha–1 produced a significantly higher yield (7.06 t ha–1) which was followed by 160 kg ha–1 (6.71 t ha–1), 140 kg ha–1 (6.30 t ha–1) and the lowest yield (3.93 t ha–1) with 0 kg N ha–1. Therefore, among the hybrids evaluated, Rampur Hybrid-2 could be an appropriate hybrid variety for the cultivation at Dang district and similar conditions in Nepal. This study suggests that maize production can be maximized by cultivating Rampur Hybrid-2 with the use of 180 kg N ha–1 in the inner Terai region of Nepal
Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers
No full textGenetic diversity assessment is the preliminary work for development of variety and conservation of diversity. Finger millet is a very important crop in Nepal however, its genetic potential has not been fully utilized. Genetic diversity was assessed in forty landraces of finger millet using 9 RAPD and 5 SSR markers. These landraces were collected from Kaski and Dhading districts. None of single primers of these RAPD and SSR could separate all 40 landraces. The average number of bands were 6.33 and 7.8 per RAPD and SSR primers respectively. Mean polymorphism information content was of 0.314 for RAPD and 0.37 for SSR. Primer OPA-4 produced the highest number of bands and the lowest numbers of bands were produced by OPA-16. Among the SSR primers, SSR-06 produced the highest number of polymorphic bands and UGEP-53 produced the lowest bands. RAPD based dendrogram has generated four clusters and SSR based dendrogram has generated two clusters. In both dendrogram and principal component analyses, Purbeli landrace was found unique locating separately in the cluster and scatter plot. Nei\u27s genetic distance produced by RAPD and SSR primers was similar that is 0.327 by RAPD and 0.296 by SSR markers. Genetic distance produced by SSR markers was higher than distance produced by RAPD marker. These landraces were from two districts and therefore have shown intermediate diversity. These molecular marker-based findings should would be more useful if we could link with agromorphological traits. Inclusion of large number of landraces collected from different areas are required to get higher level diversity in addition to associate genetic diversity with geographical sites. Groupings of these landraces could be useful for selecting landraces in breeding program as well as planning conservation program
Agro-Morphological Characterization and Estimation of Genetic Parameters of Spring Maize Hybrids in the Inner Plains of Far-West Nepal
No full textHeat resilient maize hybrids are an important input in maize cultivated area coupled with high temperature during the flowering period, with a specific focus on the Terai region of Nepal. The research was conducted in the Dhangadhi district from April 2020 to August 2020 in an alpha-lattice design with 26 genotypes and two replications. Analysis of variance suggests that genotypes were significantly different for all traits except plant height and plant population. The phenotypic coefficient of variation (PCV) was higher than the genotypic coefficient of variation (GCV) for all traits. High PCV and GCV values were found in the anthesis silking interval, ear aspect and grain yield and low values in the anthesis days and silking days. Highly heritable traits were days to 50% tasseling (0.74), days to 50% silking (0.74), ear aspect (0.69), number of rows per cob (0.84), number of grains per row (0.61), cob diameter (0.87), and cob length (0.86). Genetic advance ranged from the lowest in plant height to the highest in anthesis silking interval. Correlation studies revealed that grain yield showed a negative and significant correlation with reproductive traits, while a positive and significant correlation between ear height and number of ears per hectare was observed. Cluster analysis grouped 26 genotypes into 5 major clusters, and the intracluster distance ranged from 4034.3 to 34960.3. Thus, traits having high variation can be used as selection indices for indirect selection for the improvement of maize productivity and suggest genotype like ZH182079, ZH1767, and KH15486 with high yield for commercialization and distribution to farmers
Effect of different tillage practices and nitrogen level on wheat production under inner terai of Nepal
No full textA field experiment was conducted to evaluate the plant height, yield and yield attributes of wheat under different tillage practices and nitrogen level at Dang, Nepal during winter season 2018-19. The experiment was laid out in split plot design with two tillage practices viz. zero tillage and conventional tillage as main plot factor and four level of N viz. 50 kg ha-1, 75 kg ha-1, 100 kg ha-1and 125 kg ha-1 as sub plot factor and each replicated thrice. The result revealed that there is no significant relation between tillage practice and plant height of wheat while N level significantly affect the plant height. 125 kg N ha-1 recorded the highest plant height (110.7 cm). Zero tillage recorded the highest effective tiller m-2 (254) and grain yield (3.3 t ha-1) whereas spike length, grain spike-1, biological yield and harvest index were not significant with tillage practices. Regarding the N level, 125 kg N ha-1 recorded the highest effective spike m-2 (279), spike length (10.6 cm), grain spike-1 (48), thousand grain weight (46.3 g), grain yield (3.6 t ha-1) and biological yield (9.4 t ha-1). 50 kg N ha-1 recorded the highest harvest index (42.9%). The interaction between the tillage practice and nitrogen level showed the significant effect on grain yield and harvest index where as other parameters showed non-significant relation. The zero tillage with 125 kg N ha-1 recorded the highest grain yield (3.9 t ha-1)
Evaluation of Different Chemical Fungicides Against Rice Blast in Field Conditions
No full textEvaluation of different fungicides against rice blast was carried out in research plot of the Agronomy farm of IAAS, Prithu Technical College, Lamahi Municpality, Dang district of the Lumbini Province, inner terai region of Nepal during June to November, 2017. The objective of the experiment was to evaluate the efficiency of different chemical fungicides against rice blast. The experiment was conducted in Randomized Complete Block Design with the use of susceptible variety ‘Mansuli'. Different fungicides like Hexaconazole 5% SC (Udaan), Propiconazole 25% EC (Tilt), Captan 70% + Hexaconazole 5% WP, Validamycin 3% L, Tricyclazole 75% WP (TRIP) and Biomycin (Kasugamycin 3% S.L.) were applied five times at weekly interval with the doses of 2mL/L of H2O, 1.5mL/L of H2O, 2g/L of H2O, 2mL/L of H2O, 2g/L of H2O and 2mL/L of H2O respectively. From the result, it was concluded that all the fungicides were effective in controlling leaf blast but Tricyclazole 75% WP (TRIP) was more effective among other fungicides and untreated control plots with least leaf blast severity (27.85%), least incidence (35.5%), least mean AUDPC (64.64%) and highest grain yield (3.93 t ha-1) followed by Biomycin. It is thus concluded that fungicide Tricyclazole 75% WP should be sprayed five times at weekly interval for the management of leaf blast in rice
Chewing, Stress-Related Diseases, and Brain Function
No full textSCI(E)PubMedEDITORIAL [email protected]
RESTORATION OF MOTOR AND NON-MOTOR FUNCTIONS BY NEUROTROPHIC FACTORS IN NONHUMAN PRIMATES WITH DOPAMINE DEPLETION
No full textParkinson’s disease (PD) is a progressive debilitating neurodegenerative disorder characterized by resting tremor, rigidity, bradykinesia and postural instability. As the disease progresses there is a loss of dopamine (DA) neurons in the substantia nigra projecting to the various forebrain and sub-cortical regions. Current treatments for PD are unable to prevent or curtail the neurodegenerative process; so rescuing remaining dopamine in the mid-brain has been the recent focus of research examining the effectiveness of neurotrophic factors (NTFs) in the treatment of PD. In this dissertation, the ability of three novel, recently discovered NTFs to restore DA neurons and motor function in a nonhuman primate model of PD was examined. The NTFs were Cerebral Dopamine Neurotrophic Factor (CDNF) and two variants of Neurturin (NRTN), N2 and N4, that have mutations that prevent binding to heparin sulfate binding sites in the brain. These studies used the unilateral low dose (0.15 ± 0.001 mg/kg) monkey 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of PD to cause loss of DA neurons. Six groups of monkeys were studied: vehicle-treated (negative control), Glial Cell-line Derived Neurotropic Factor (GDNF, positive control), two groups of CDNF-treated monkeys (450 μg and 150 μg), and N2 and N4-treated groups. After MPTP, monkeys developed moderate symptoms of PD (PD rating scale score=7.9±0.5 on a scale of 0-22, p<0.001), motor dysfunction and increased daytime sleepiness. After three months of infusions, all three NTFs (150 μg CDNF, N2 and N4) significantly increased the number of DA neurons in the substantia nigra, p=0.03, and improved parkinsonian symptoms measured by rating scale, p<0.001. Most motor functions were significantly correlated with the number of DA neurons in the substantia nigra. N4 significantly improved daytime sleep duration, bouts and wake-latency (p=0.02, p=0.06 and p=0.02, respectively). In summary, CDNF, N2 and N4 trophic factors are neurorestorative to DA neurons, motor function is tightly correlated with DA neuronal number, and N4 improved the non-motor symptom of increased daytime sleepiness in this monkey PD model. These factors hold promise for clinical therapy for PD patients
