7,787 research outputs found
Oral history interview with Tracy Jones, April 20, 2001
No full text1 electronic record(s) and derivatives. 1 audio file (wav, mp3) 757.95 MB. 00:33:29. 1 PDF documents (5 scans, jp2). 908 MB (952,824,383 byteOral history interview with Tracy Jones, April 20, 2001. Valdosta (Ga.). Fieldworker: Lynita Hamilton. Audio file digitized from cassette tape. Part of the South Georgia Folklife Project at Valdosta State University Archives and Special Collections. Topics include gospel music and Cherokee ancestry
Abstract 2427: Alterations in gene expression after exposure of MDA-MB-231 cells to isobutylparaben
No full textAbstract
Parabens are a group of chemical compounds extensively used as preservatives in consumer products such as cosmetics, pharmaceuticals and processed food. Parabens have been shown to have endocrine disrupting properties, to accumulate in breast tissue and to increase the proliferation of hormone receptor positive breast cancer cell lines through competitive binding to the estrogen receptor. Studies have shown that isobutylparaben has the highest binding affinity for the aryl hydrocarbon receptor (AhR), reported to bind environmental toxins. This study examined the alterations in gene expression correlated to exposure of the hormone receptor negative breast cancer cell line MDA-MB-231 to isobutylparaben over a 72 hour time frame. Exposure of the cells to concentrations of isobutylparabens commonly found in consumer products alters the genetic expression profile of the cells. In our study, detectable expression levels of AhR mRNA decreased, while cytochrome P450 (CYP1A1) and receptor activator of NFκB ligand (RANKL) mRNA levels increased, as analyzed by qPCR. The downregulation of AhR and altered expression levels of invasion related genes is suggestive of an increased metastatic phenotype with exposure to isobutylparaben.
Citation Format: Christine Strelchuk, Holly Jones Taggart. Alterations in gene expression after exposure of MDA-MB-231 cells to isobutylparaben [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 2427. doi:10.1158/1538-7445.AM2017-2427</jats:p
Hugh-Jones, Philip: transcript of an audio interview (05-Jul-2000)
No full textInterview with Dr Philip Hugh-Jones, conducted by Dr Andy Ness, for the History of Twentieth Century Medicine Research Group, UCL, 05 July 2000. Transcribed by Mrs Jaqui Carter, and edited by Professor Tilli Tansey and Dr Hugh Thomas. Dr Philip Hugh-Jones BA Cantab MB BChir MD MRCP FRCP (1917-2010) was Consulting Physician and Director of the Chest Unit at King’s College Hospital, London, and Consulting Physician and part-time Director of the MRC Clinical Pulmonary Physiology Research Unit at Hammersmith Hospital, London, from 1964 to 1967 when it was taken over by the University of London. He had been on the scientific staff of the MRC Pneumoconiosis Unit from 1945 to 1952.The History of Modern Biomedicine Research Group is funded by the Wellcome Trust, which is a registered charity (no. 210183). The current interview was funded by a Wellcome Trust grant (059533; 1999-2001; awarded to Professor G Davey-Smith, Dr A R Ness and Dr E M Tansey), and its publication by a Wellcome Trust Strategic Award entitled “Makers of modern biomedicine: testimonies and legacy” (2012-2017; awarded to Professor Tilli Tansey)
Evaluation of dysregulation of the receptor tyrosine kinases Kit, Flt3, and Met in histiocytic sarcomas of dogs
No full textReceiver Windowing Design for Narrowband Interference Mitigation in MB-OFDM UWB System
No full textIn 2005, the WiMedia Alliance working with the European Computer Manufacturers Association (ECMA) announced the establishment of the WiMedia MB-OFDM (Multiband Orthogonal Frequency Division Multiplexing) UWB radio platform as their global UWB standard. It was also chosen as the physical layer (PHY) of high data rate wireless specifications for high speed Wireless USB (W-USB), Bluetooth 3.0 and Wireless High-Definition Media Interface (HDMI). However, due to the low power and wide bandwidth nature of UWB systems, in-band narrowband interference (NBI) may hinder the receiver performance. This thesis presents an analysis of NBI impact on the MB-OFDM system for UWB communication. The intent of our analysis is to provide practical solutions for interference mitigation under different NBI models. In our work, a new receiver windowing for zero padding (ZP) OFDM system is proposed to reduce NBI spreading in the MB-OFDM UWB system. Simulations demonstrate the effectiveness of windowing under different NBI models.Microelectronics & Computer EngineeringElectrical Engineering, Mathematics and Computer Scienc
Altered immunolocalization of FGF23 in murine femora metastasized with human breast carcinoma MDA-MB-231 cells
No full textIntroduction After the onset of bone metastasis, tumor cells appear to modify surrounding microenvironments for their benefit, and particularly, the levels of circulating fibroblast growth factor (FGF) 23 in patients with tumors have been highlighted. Materials and methods We have attempted to verify if human breast carcinoma MDA-MB-231 cells metastasized in the long bone of nu/nu mice would synthesize FGF23. Serum concentrations of calcium, phosphate (Pi) and FGF23 were measured in control nu/nu mice, bone-metastasized mice, and mice with mammary gland injected with MDA-MB-231 cells mimicking primary mammary tumors. Results and conclusions MDA-MB-231 cells revealed intense FGF23 reactivity in metastasized lesions, whereas MDA-MB-231 cells cultured in vitro or when injected into the mammary glands (without bone metastasis) showed weak FGF23 immunoreactivity. Although the bone-metastasized MDA-MB-231 cells abundantly synthesized FGF23, osteocytes adjacent to the FGF23-immunopositive tumors, unlike intact osteocytes, showed no FGF23. Despite significantly elevated serum FGF23 levels in bone-metastasized mice, there was no significant decrease in the serum Pi concentration when compared with the intact mice and mice with a mass of MDA-MB-231 cells in mammary glands. The metastasized femora showed increased expression and FGFR1 immunoreactivity in fibroblastic stromal cells, whereas femora of control mice showed no obvious FGFR1 immunoreactivity. Taken together, it seems likely that MDA-MB-231 cells synthesize FGF23 when metastasized to a bone, and thus affect FGFR1-positive stromal cells in the metastasized tumor nest in a paracrine manner
ASIC FFT processor for MB-OFDM UWB system
No full textThe physical layer (PHY) standard of Multi-band Orthogonal Frequency Division Multiplexing (MB-OFDM) Ultra Wideband (UWB) system was defined by ECMA International. In this standard, the data sampling rate from the analog-to-digital converter to the physical layer is up to 528 Msample/s. Therefore, it is a challenge to realize the physical layer of the UWB system-especially the components with high computational complexity in Very Large Scale Integration (VLSI) implementation. Fast Fourier Transform (FFT) block is one of these components. FFT plays an important role in Multi-band OFDM UWB system, which is the demodulation block of OFDM signals. The purpose of this project is to design an Application Specific Integrated Circuit (ASIC) FFT solution for this system. The specification is defined from the system analysis and literature research. All the design choices and considerations are concluded and explained.Based on the algorithm and architecture analysis, a novel Radix22Parallel processor is proposed, which is a small-area and low-power-consumption solution for MB-OFDM UWB system. Both Field Programmable Gate Array (FPGA) and ASIC targeted synthesis results of this architecture are presented.Electrical Engineering, Mathematics and Computer Scienc
8.07.013: Letter relating to Adventures of Faustus Bidgood, 1991
No full textThis letter and enclosed documents explain the distribution and investor revenue calculations for the Andy Jones film The Adventure of Faustus Bidgood (1986)
Metastasis of MDA-MB-231/GFP and MDA-MB-231/GFP+ASC/RFP tumors.
No full text<p>40 days after subcutaneous injection of either human MDA-MB-231/GFP cells, ASC/RFP cells or MDA-MB-231/GFP+ASC/RFP cells, mouse organs were collected. <b>A.</b> Visual macrometastatic lesions were observed in the liver, lungs only in mice co-injected with MDA-MB-231/GFP and ASC/RFP (arrows). <b>B.</b> H&E sections of the liver and lungs of mice bearing MDA-MB-231/GFP+ASC/RFP tumors showing metastatic MDA-MB-231 cancer cells (insets). <b>C.</b> To quantitate micrometastases, DNA was prepared from mouse organs from two separate experiments (n = 10 mice/group) for detection of human chromosome 17 by real time RT-PCR. A significant increase in micrometastasis for MDA-MB-231/GFP+ASC/RFP tumors was detected in liver, lung and spleen. * P<0.05.</p
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