1,725 research outputs found
Johnson Brothers Company
No full textHerman Hansen was the local manager of the Johnson Brothers and Company General Merchandise store in South Cle Elum about the time this L. B. Waters photograph was taken. The store\u27s slogan What you buy we stand by advertised Johnson Brothers commitment to local customers.Postcard titled: So. Cle Elum, WN. L. B. Waters, Photo.https://digitalcommons.cwu.edu/ellensburg_history/1135/thumbnail.jp
Toward monitoring and estimating the size of the HFO-contaminated seabed around a shipwreck using MBES backscatter data
No full textFunding Information: The authors are grateful to three anonymous reviewers for their constructive criticism and valuable comments. The authors express their gratitude to the Maritime Institute in Gdansk for making the final version of the report (Maritime Institute in Gdansk, 2016) available. The second author appreciates the support of the Gdynia Maritime University in funding this research through internal grant WN/PZ/2021/02. Funding Information: The second author appreciates the support of the Gdynia Maritime University in funding this research through internal grant WN/PZ/2021/02 . Publisher Copyright: © 2021Despite a progressive reduction of oil spills caused by the activity of maritime transportation, the latent sources of pollution still exist. Although the harmful impact of heavy fuel oil (HFO) on the marine environment is widely known, many shipwrecks cause contamination of the surrounding areas. In this paper, an approach to monitor the area of the HFO spill around a shipwreck is made using a bottom backscattering strength (BBS) obtained by a multibeam echosounder (MBES). As a case study, the s/s Stuttgart wreck located in the Gulf of Gdansk (Poland) is verified. Two different measurement campaigns have been carried out in shallow waters using low (190 kHz) and high (420 kHz) MBES frequency. The results indicate that the polluted area around s/s Stuttgart was estimated at 49.1 ha, which is around 18.3% more in comparison to the geological surveys made four years earlier.Peer reviewe
MDM2 negatively regulates the human telomerase RNA gene promoter
No full text<b>Background:</b>
We have previously demonstrated that NF-Y and Sp1 interact with the human telomerase RNA (hTR) promoter and play a central role in its regulation. We have also shown that pRB activates the hTR promoter, but the mechanism of pRb directed activation is unknown. It has recently been reported that pRB induces Sp1 activity by relieving inhibition mediated by mdm2. The aim was to investigate possible roles for mdm2 in hTR promoter regulation.
<b>Methods:</b>
Chromatin immunoprecipitation was used to determine binding of mdm2 to the hTR promoter. Transfection and luciferase assays were used to investigate mdm2 repression of the promoter activity and interaction with known transcriptional modulators.
<b>Results:</b>
Here we show using chromatin immunoprecipitation that mdm2 specifically binds the hTR promoter in vivo. Transient co-transfection experiments using an hTR promoter luciferase reporter construct show that hTR promoter activity is inhibited by over-expression of mdm2 in 5637 bladder carcinoma cells (p53 and pRB negative, low mdm2). Titration of mdm2 was able to antagonise activation of hTR promoter activity mediated by pRB or Sp1 over-expression, although in the presence of pRB, mdm2 could not repress promoter activity below basal levels. Using an Sp1 binding site mutation construct we showed that mdm2 repression did not absolutely require Sp1 binding sites in the hTR promoter, suggesting the possibility of pRB/Sp1 independent mechanisms of repression. Finally, we show that NF-Y mediated transactivation of the hTR promoter was also suppressed by mdm2 in a dose-dependent manner.
<b>Conclusions:</b>
These studies suggest that mdm2 may inhibit the hTR promoter by multiple mechanisms. Mdm2 may directly repress activation by both pRB and Sp1, or activation by NF-Y. Furthermore, the ability of mdm2 to interact and interfere with components of the general transcription machinery might partly explain the general repressive effect seen here. Elucidation of new regulators affecting hTR basal promoter activity in cancer cells provides a basis for future studies aimed at improving our understanding of the differential hTR expression between normal and cancer cells
The Weight and Hopcount of the Shortest Path in the Complete Graph with Exponential Weights
No full textBoth the hopcount HN (the number of links) and the weight WN (the sum of the weights on links) of the shortest path between two arbitrary nodes in the complete graph KN with i.i.d. exponential link weights is computed. We consider the joint distribution of the pair (HN,WN) and derive, after proper scaling, the joint limiting distribution. One of the results is that HN and WN, properly scaled, are asymptotically independent.Delft Institute of Applied MathematicsElectrical Engineering, Mathematics and Computer Scienc
Feasibility of abdominoplasty with Cesarean section [Retraction]
No full textThe Editor-in-Chief and Publisher of the International Journal of Women&rsquo;s Health have been alerted by Dr Nadine Sherif, the corresponding author, to unacceptable levels of duplication with a previously published paper: Ali A, Essam A. Abdominoplasty Combined with Cesarean Delivery: Evaluation of the Practice. Aesthetic Plastic Surgery. 2011;35(1):80&ndash;86.It is worth noting that this paper was peer-reviewed by two peer-reviewers and the Editor-in-Chief of the International Journal of Women&rsquo;s Health before publication. The paper concerned is: Thabet WN, Hossny AS, Sherif NA. Feasibility of abdominoplasty with Cesarean section. International Journal of Women&rsquo;s Health. 2012;4:115&ndash;121
A concept of critical safety area applicable for an obstacle-avoidance process for manned and autonomous ships
No full textFunding Information: This research work was supported by the Gdynia Maritime University internal grant WN/PZ/2021/02. Funding Information: The author is grateful to three anonymous reviewers for their constructive criticism and valuable comments. The author expresses his gratitude to Prof. Jerzy Matusiak of Aalto University for making the LaiDyn code available, as well as to Dr. Tomasz Hinz of Waterborne Transport Innovation Foundation for preparing LaiDyn output files containing ship trajectories. This research work was supported by the Gdynia Maritime University internal grant WN/PZ/2021/02. Publisher Copyright: © 2021 The Author(s)In times of increased automation of maritime transportation, ship collision with a stationary obstacle (allision) remains a significant problem. There are many existing solutions rooted primarily in the concept of ship domain and path-planning algorithms. However, among these, a geometrical approach to the determination of a required maneuvering area considering the dynamic nature of ship operations in close-quarters situations is still missing. Therefore, an improved concept of the CADCA (Collision Avoidance Dynamic Critical Area) is introduced for the case of ship allision. The CADCA is a deterministic zone that geometrically delimits required maneuvering space of a vessel. Its shape changes depending on the operational parameters of a ship, such as the magnitude of rudder angle, initial forward speed, or planned alteration of the course. In contrast to ship domain, the CADCA is determined using the critical distance between two objects called MDTC (Minimum Distance to Collision). Therefore, the CADCA concept can be used to appoint a position of no-return in a close-quarters situation, so as to determine the time and distance of the last-minute maneuver. An improved method of CADCA determination is introduced, along with an investigation of operational factors influencing the ship's critical area in allision scenarios. The simulations have been conducted for large passenger and container ship in encounters with various stationary obstacles differing in size and shape. The results indicate that from the operational point of view, a deflection of the rudder is the most influencing factor on the size of the CADCA, while the impact of ship speed is negligible for the investigated vessels. Besides, various applications of the CADCA are proposed and discussed for both manned and prospective autonomous vessels.Peer reviewe
Emerg Infect Dis
No full textA traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and avian tissues
Guatteria liesneri D. M. Johnson
No full text[63] <i>Guatteria liesneri</i> D.M.Johnson & N.A.Murray <p> <i>Ann. Missouri Bot. Gard.</i> 77 (3): 598 (Johnson & Murray 1990).</p> <p> <i>Guatteria anthracina</i> Scharf & Maas, <i>Blumea</i> 51 (1): 118 (Scharf & Maas 2006).</p> <p>VERNACULAR NAMES. — Wn: kaloj ëwa, oka epu • Nt: uku tiki.</p> <p> HERBARIUM DATA (FG). — 17 collections at CAY. Sel. exs.: <i>D. Sabatier & M.-F. Prévost 3660</i>.</p> <p>INVENTORY DATA (FG). — 16 trees in 11 plots; Fmax <1 %; dbhinv = 35.7 cm.</p>Published as part of <i>Molino, Jean-François, Sabatier, Daniel, Grenand, Pierre, Engel, Julien, Frame, Dawn, Delprete, Piero G., Fleury, Marie, Odonne, Guillaume, Davy, Damien, Lucas, Eve J. & Martin, Claire A., 2022, An annotated checklist of the tree species of French Guiana, including vernacular nomenclature, pp. 345-903 in Adansonia (3) (3) 44 (26)</i> on page 374, DOI: 10.5252/adansonia2022v44a26, <a href="http://zenodo.org/record/7458777">http://zenodo.org/record/7458777</a>
Athoplastus palouse Etzler and Johnson 2018, new species
No full text<i>Athoplastus palouse</i> Etzler and Johnson, new species <p>(Adult: Figs. 2, 14, 25–27)</p> <p>Zoobank.org/ urn:lsid:zoobank.org:act: 9601386F-4698-4376-8144-FB8FED38DAB2</p> <p> <b>Diagnosis.</b> This species can be distinguished from other <i>Athoplastus</i> species by the combination of the non-compressed prosternum (Fig. 8), dense pronotal punctation (Fig. 10), and elytra without dark markings along the striae of the elytra. More subtle characters that help distinguish this species are the more parallel-sided pronotum (Fig. 2) and the slightly wider antennomeres (Fig. 14). These last two characters are best used when comparing specimens of different species and become quite clear in this way. However, when identifying just a single specimen, these characters are less useful, so the characters from the key should be used to eliminate other potential species. This species is quite similar to <i>A. rainieri</i>, but the paramere tips and V-shaped membranous area of the basal piece of the male genitalia (Figs. 25–27) and geographic distribution, in addition to the subtle characters above, will distinguish these two species.</p> <p> <b>Description. Male.</b> Length 15–17 mm in length, width 3–4 mm (measured across bases of elytra). Red-brown to brown, carina, palps, and tips of mandibles darker; posterior margin of hypomeron, coxae, and tarsi lighter, almost tawny yellow (Fig. 2). <b>Head:</b> Ocular index = 60.0–68.4 (mean = 65.0, <i>n</i> = 3). <b>Antennae:</b> Mean ratio of antennomeres 2- 11: 1: 1.25: 3.4: 3.6: 4; 4: 3.8: 4: 5.2 (<i>n</i> = 2). [Note: Antennomeres 8–11 are adjusted values based only on one specimen. The antennomeres, on average, are less narrowed than in other species. This is difficult to describe, so compare Fig. 14 to Figs. 12 and 13.] <b>Pronotum:</b> 1.4–1.6X wider than long. Lateral margin completely carinate, subparallel anterad divergent hind angles; subtly dorsoventrally flattened along lateral edges near anterior fourth. Punctures distinct, simple and dense on disc, separated by at most 1 diameter, often by about 0.5X diameter of puncture; punctures becoming denser along margins, nearly continuous laterally. <b>Prosternum:</b> Slightly convex with dense, shallow, subumbilicate punctures; anterior lobe short, broadly rounded, shallowly deflexed. Posterior process short and arcuate, gradually raised ventrally between procoxae, then deflexed posteriorly. <b>Legs:</b> Tarsomere 5 equal to tarsomere 1 on all tarsi. <b>Elytra:</b> Elongate, subparallel, 4.0–4.5X pronotal length; striae shallowly impressed, deeper apically; intervals flat, punctures fine, shallow, and irregularly distributed. <b>Aedeagus</b> (Figs. 25–27): Basal piece 2/5 total length, with broadly triangular membranous section on ventral surface (Fig. 26); median lobe 1.3X length of parameres, slightly deflexed ventrally at tip, median carina on ventral surface, basal struts following edges of median lobe; parameres wide basally, edges slightly sinuate and gradually narrowing toward tip, tip with very acute lateral expansion bearing 2 setae (Fig. 27).</p> <p> <b>Female.</b> Unknown.</p> <p> <b>Specimens Examined (8).</b> <b>Holotype. USA: WASHINGTON:</b> [Whitman Co.], Pullman, 7.vi. [18]98, C. V. Piper (1, WSUC). <b>Paratypes. USA: IDAHO:</b> Kootenai Co., Athol, 29.iv.1957, W. F. Barr Collector (1, WFBM). <b>WASHINGTON:</b> Washington T[erritory] (1, USNM); Pullman, Sept. [year unknown] (1, USNM); [Whitman Co.], Pullman, 8.vi.[18]99 (1, WSUC); Pullman, 31.iii. [19]11 (1, WSUC); Pullman, 01.iv.[19]17 (1, USNM); Pullman, Apr [il] [19]20, Chester Dixon (1, USNM); Kamiak Butte, 23.ii.[19]34 Wn, J.F. Clarke (1, USNM).</p> <p> <b>Distribution.</b> This species is only known from the areas of Pullman, Washington and Athol, Idaho (Fig. 29).</p> <p> <b>Etymology.</b> The specific name “ <i>palouse</i> ” is a noun in apposition derived from the geographic area of collection of the majority of specimens.</p> <p> <b>Taxonomic Remarks</b>. The distance between localities is quite a surprise, but the morphology of specimens from the two localities is consistent. <i>Athoplastus palouse</i> may co-occur with <i>A</i>. <i>acutus</i>.</p>Published as part of <i>Etzler, Frank E. & Johnson, Paul J., 2018, Athoplastus Johnson and Etzler (Coleoptera: Elateridae: Dendrometrinae), a New Genus of Click Beetle from the Northwestern Continental USA, pp. 503-521 in The Coleopterists Bulletin 72 (3)</i> on pages 516-517, DOI: 10.1649/0010-065X-72.3.503, <a href="http://zenodo.org/record/4789344">http://zenodo.org/record/4789344</a>
Emerg Infect Dis
No full textAfter the 1999 outbreak of West Nile (WN) encephalitis in New York horses, a case definition was developed that specified the clinical signs, coupled with laboratory test results, required to classify cases of WN encephalitis in equines as either probable or confirmed. In 2000, 60 horses from seven states met the criteria for a confirmed case. The cumulative experience from clinical observations and diagnostic testing during the 1999 and 2000 outbreaks of WN encephalitis in horses will contribute to further refinement of diagnostic criteria
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