1,721,517 research outputs found
New protein identification in PRRSV
No full textMurtaugh, Michael; Johnson, Craig; Griggs, Theodor; Robinson, Sally; Gnanandarajah, Josephine. (2011). New protein identification in PRRSV. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/140470
Canine chondrocytes and osteoblasts cyclically compressed
No full textCells are agarose embedded and cyclically compressed on FXC compression bioreactor for 4 hours followed by 24 hours of recovery incubation.
RNA extracted according to Bougault et al (2008) and canine microarray performe
Stimulation of canine joint cells to model osteoarthritis
No full textCanine chondrocytes and osteoblasts were embedded in an agarose matrix and simultaneously stimulated with cytokines whilst being cyclically compressed. Both cell types were compressed for 4 hours at 1 Hz followed by a 24 hour recovery incubation.
The chondrocytes were stimulated with 5 ng/mL of IL8 for the duration of compression, but removed for the recovery phase.
Osteoblasts were stimulated with 0.5 ng/mL IL8 and 0.5 ng/mL IL1b for the duration of the compression and for the entirety of the 24 hour incubation step
Canine medial coronoid process disease microarray data
No full textThe objective of this study was to identify differentially expressed genes associated with the process of medial coronoid process disease, primary to the development of osteoarthritis. The data were produced using Affymetrix Canine Gene Chips and relate to RNA from 5 control vs 5 diseased samples in cartilage, and 5 control vs 5 diseased samples in bone (as annotated in the upload). The samples are from canine elbows, and individual case data might be available upon request.Files are .CEL format produced during Affymetrix Microarray Experiments. It is a widely used format for these experiments. A number of software packages are capable of opening these files, including Affymetrix Expression Console and Partek Genomics Suite
Joint Cells Stimulated with Cytokines (As in abstract)
No full textIn vitro modelling of osteoarthritis typically uses cytokines to induce a catabolic phenotype in joint cells. This model was used to identify the response to a cytokine parameter identified as most representative of early stage osteoarthritis. Canine cells were used, isolated from the humeral head of post-mortem subjects. The cartilage cells were stimulated with 5 ng/ml of IL8 for 2 hours, whilst the osteoblasts were stimulated with 500 pg/mL of IL1 and 500 pg/mL of IL8. After the indicated culture time, RNA was extracted and a microarray was performed to identify gene expression changes.Files are .CEL format produced during Affymetrix Microarray Experiments. It is a widely used format for these experiments. A number of software packages are capable of opening these files, including Affymetrix Expression Console and Partek Genomics Suite
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
A summary of PRRS virus diagnostic and research data
No full textMurtaugh, Michael; Finnegan, Colleen; Fuentes, Martha; Johnson, Craig; Yu, Wanqin; Abrahante, Juan; Kerkaert, Barry; Spronk, Gordon; Wagner, Mark; Fitzsimmons, Mark. (2006). A summary of PRRS virus diagnostic and research data. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/141959
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