616 research outputs found
Labilibacter aurantiacus gen. nov., sp nov., isolated from sea squirt (Styela clava) and reclassification of Saccharicrinis marinus as Labilibacter marinus comb. nov.
No full textA Gram-stain-negative, facultatively anaerobic, orange-pigmented bacterium, designated HQYD1(T), was isolated from a sea squirt (Styelaclava) and characterized using a polyphasic approach. Morphologically, strain HQYD1(T) exhibited rods with gliding motility. This novel isolate grew optimally at 28 degrees C in the presence of 2-3% (w/v) NaCl. The 16S rRNA gene sequence was most similar to [Saccharicrinis] marinus Y11(T) (96.3 %), followed by Saccharicinis fermentans DSM 9555(T) (93.8 %). The dominant fatty acids of strain HQYD1(T) were identified as C-16 : 0, C-18 : 0 and iso-C-15 : 0. Major polar lipids included an unidentified lipid and a phospholipid. The major respiratory quinone was found to be MK-7, and the genomic DNA G+C content was determined to be 35.1 mol%. Based on evidence from this taxonomic study, a novel genus, Labilibacter gen. nov., is proposed in the family Marinilabiliaceae with type species Labilibacter aurantiacus sp. nov. The type strain of the type species is HQYD1(T) (=MCCC 1K02304(T)=KCTC 42583(T)). As [Saccharicrinis] marinus Y11(T) clustered phylogenetically with strain HQYD1(T), we also propose [Saccharicrinis] marinus Y11(T) be reclassified as Labilibacter marinus comb. nov. (type strain Y11(T)=CICC 10837(T)=KCTC 42400(T))
Immunocytochemical localization of thyroglobulin in the endostyle of the anadromous sea lamprey, Petromyzon marinus L
No full textThyroglobulin (TG) was localized in the endostyle of the anadromous sea lamprey, Petromyzon marinus L. by means of the unlabeled antibody peroxidase-antiperoxidase immunocytochemical method. TG was found localized on the apical surface and within the cytoplasm of type 2c and 3 cells and in some type 5 cells. By identifying the cells of the endostyle immunocytochemically it may be possible to study more readily the events of endostylar transformation during metamorphosis
History of the Old Dutch Church at Totowa, Paterson, New Jersey, 1755 to 1827: with Baptismal Register 1756-1808
No full textHistory of the church which was located at what is now Ryle Avenue, Paterson. Description of the church buildings and historical anecdotes. Biography of first pastor, Dominie David Marinus, who was the first person ordained by the Coetus, founding body of the Reformed Church in America. Members of original consistory, and lists of pew holders from 1816 and circa 1821. Transcription of original call for pastor, in Dutch, from 1756, and baptismal register, in Dutch and English, from 1756-1808.200 copies printe
The Perfect Storm: The Political Economy of the Fischler Reforms of the Common Agricultural Policy. CEPS Paperbacks. September 2008
No full textFor decades, the Common Agricultural Policy came in for a significant amount of criticism for consuming a disproportionate share of the EU budget, introducing market distortions, wasting government funds and contributing to rural inequities. Nevertheless, it survived many attempts to abolish it, and acquired a reputation for being virtually impossible to reform in any meaningful way. However, during the tenure of Franz Fischler as European Commissioner for Agriculture from 1995 to 2004, the most radical reform in the history of the CAP was implemented.
This book is the first to review the reforms that were implemented, to analyse how they came about and to explain which forces made them possible. It brings together perspectives from inside and outside the policy community, including from those closely involved in the policy debates, and an interdisciplinary perspective from economists and political scientists. The authors are senior policy-makers and well-respected academics. The book gives some fascinating insights into what made the reforms possible, offers useful conclusions on what this implies for future attempts at reform and finally, addresses the question of whether the Fischler reforms ‘scrapped the CAP’ or saved it
Characterization of a thermophilic 4-O-β-d-mannosyl-d-glucose phosphorylase fromRhodothermus marinus
No full text4-O-β-d-Mannosyl-d-glucose phosphorylase (MGP), found in anaerobes, converts 4-O-β-d-mannosyl-d-glucose (Man-Glc) to α-d-mannosyl phosphate and d-glucose. It participates in mannan metabolism with cellobiose 2-epimerase (CE), which converts β-1,4-mannobiose to Man-Glc. A putative MGP gene is present in the genome of the thermophilic aerobe Rhodothermus marinus (Rm) upstream of the gene encoding CE. Konjac glucomannan enhanced production by R. marinus of MGP, CE, and extracellular mannan endo-1,4-β-mannosidase. Recombinant RmMGP catalyzed the phosphorolysis of Man-Glc through a sequential bi–bi mechanism involving ternary complex formation. Its molecular masses were 45 and 222 kDa under denaturing and nondenaturing conditions, respectively. Its pH and temperature optima were 6.5 and 75 °C, and it was stable between pH 5.5–8.3 and below 80 °C. In the reverse reaction, RmMGP had higher acceptor preferences for 6-deoxy-d-glucose and d-xylose than R. albus NE1 MGP. In contrast to R. albus NE1 MGP, RmMGP utilized methyl β-d-glucoside and 1,5-anhydro-d-glucitol as acceptor substrates
Light and electron microscopic immunocytochemical localization of thyroglobulin in the thyroid gland of the anadromous sea lamprey, Petromyzon marinus L., during its upstream migration
No full textAntibodies made against thyroglobulin (TG) were used in an immunocytochemical study for the light and electron microscopic localization of TG in the thyroid gland of the anadromous sea lamprey, Petromyzon marinus, during its upstream migration. TG was found in the follicular lumen and in some colloid droplets within the follicular cells. Except for an immunoreactive product observed in a small portion of the interstitial connective tissue, the location of TG in the lamprey was similar to that in the thyroid of the rat
Serum thyroxine concentrations in larval and metamorphosing anadromous sea lamprey, Petromyzon marinus L
No full textThyroxine (T4) concentrations in samples of fresh sera taken from fed and unfed larval (ammocoetes) and metamorphosing anadromous sea lamprey (Petromyzon marinus) were measured by means of radioimmunoassay. T4 levels in unfed ammocoetes after a 1-month period were not significantly different from those of the fed ammocoetes. Nutritional state of ammocoetes therefore does not seem to affect their serum T4 levels. Serum T4 concentrations decreased abruptly at the onset of metamorphosis and declined to levels observed in spawing adults (Leloup and Hardy, '76; Packard et al., '76) by the middle (stage 3) of metamorphosis. No significant change in serum T4 levels occurred between stage 3 to the end of metamorphosis. The possible significance of decline in serum T4 levels to the metamorphic event in lamprey is discussed
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