12,882 research outputs found
Nuclear speckle dynamics and function of speckle association in gene expression
No full textNuclear speckles have been studied intensively for a long time. Accumulating evidence has suggested that nuclear speckles have a dynamic structure, and are associated with a significant fraction of active chromosome regions and genes. However, their mobility and the regulation mechanism of their number and size are still poorly understood. Importantly, the functional significance of the speckle-gene association also remains unclear. To understand speckle dynamics and effects of speckle-gene association on gene expression better, I study the mobility of nuclear speckle bodies and its effect on morphological change of speckle size in chapter 2, and transcription enhancement by speckle-gene association in chapter 3.
In chapter 2, I show significantly increased mobility of nuclear speckles after transcriptional inhibition, including long-range directed motion of one speckle towards another speckle, terminated by speckle fusion, over distances up to 4 µm and with velocities between 0.2-1.5 µm/min. Frequently, 3 or even 4 speckles follow very similar paths, with new speckles appearing along the path followed by a preceding speckle. Speckle movements and fusion events contribute to the formation of fewer but larger speckles after transcriptional inhibition. These speckle movements are not actin-dependent, but occur within chromatin-depleted channels enriched with small granules containing the speckle-marker protein SON. Our observations suggest a mechanism for long-range, directed nuclear speckle movements, contributing to the overall regulation of nuclear speckle number and size as well as the nuclear organization.
In chapter 3, I show nuclear speckle association results in several-fold transcriptional amplification of Hsp70 genes. Hsp70 BAC transgenes and endogenous genes turn on 2-4 mins after heat shock irrespective of their distance to nuclear speckles. However, I observe 12-56-fold and 3-7-fold higher transcription levels for speckle-associated Hsp70 transgenes and endogenous genes, respectively, after 1-2 hrs heat shock. Several-fold higher transcription levels for several genes flanking the Hsp70 locus also correlate with speckle-association at 37 ℃. Live-cell imaging reveals this modulation of Hsp70 transcription temporally correlates with speckle association/disassociation. Our results demonstrate stochastic gene expression dependent on positioning relative to a liquid-droplet nuclear compartment enriched in RNA processing and transcription-related factors through a “transcriptional amplification” mechanism, which is distinct from transcriptional bursting.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-12-01The student, Jiah Kim, accepted the attached license on 2018-11-28 at 11:39.The student, Jiah Kim, submitted this Dissertation for approval on 2018-11-28 at 11:47.This Dissertation was approved for publication on 2018-11-30 at 11:31.DSpace SAF Submission Ingestion Package generated from Vireo submission #13133 on 2019-02-07 at 14:18:29Made available in DSpace on 2019-02-07T20:36:03Z (GMT). No. of bitstreams: 3
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Previous issue date: 2018-11-30Embargo set by: Seth Robbins for item 109837
Lift date: 2021-02-07T20:36:09Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 109837
Lift date: 2021-02-07T20:39:46Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 109837
Lift date: 2021-02-07T20:44:35Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemU of I Only Restriction Lifted for Item 109837 on 2021-02-08T10:15:18Z
sj-docx-1-msj-10.1177_13524585211060326 – Supplemental material for Brighter spotty lesions on spinal MRI help differentiate AQP4 antibody-positive NMOSD from MOGAD
No full textSupplemental material, sj-docx-1-msj-10.1177_13524585211060326 for Brighter spotty lesions on spinal MRI help differentiate AQP4 antibody-positive NMOSD from MOGAD by Jae-Won Hyun, Hye Lim Lee, Jaehong Park, Jiah Kim, Ju-Hong Min, Byoung Joon Kim, Seung Woo Kim, Ha Young Shin, So-Young Huh, Woojun Kim, Ji Won Seo, Ki Hoon Kim, Su-Hyun Kim and Ho Jin Kim in Multiple Sclerosis Journal</p
Author Correction: Evaluation of skin cancer resection guide using hyper‑realistic in‑vitro phantom fabricated by 3D printing
No full textThe original version of this Article contained an error in the spelling of the author Taehun Kim which was incorrectly given as Teahun Kim. The original Article has been corrected
DBLP-derived labeled data for author name disambiguation
No full textThis is a DBLP-derived labeled data originally created by Dr. C. Lee Giles at Penn State University and filtered for duplicate removal and error correction by Dr. Jinseok Kim at University of Michigan. For more details, see references below.1. Kim, Jinseok (2018). Evaluating author name disambiguation for digital libraries: a case of DBLP. Scientometrics. doi:10.1007/s11192-018-2824-5 2. Kim, Jinseok & Kim, Jenna (2018). The impact of imbalanced training data on machine learning for author name disambiguation. Scientometrics. doi: 10.1007/s11192-018-2865-9Each row refers to an author name instance with following feature information separated by tab.author name: full name string extracted from DBLPunique author id: labels assigned manually by Dr. C. Lee Giles's teampaper id: assigned by Dr. Jinseok Kimauthor list: names of authors in the byline of the paperyear: publication yearvenue: conference or journal namestitle: stopwords removed and stemmed by the Porter's stemmerIf you want to use this dataset, please consider to cite papers below.For the original dataset: Han, H., Giles, L., Zha, H., Li, C., & Tsioutsiouliklis, K. (2004). Two Supervised Learning Approaches for Name Disambiguation in Author Citations. JCDL 2004: Proceedings of the Fourth ACM/IEEE Joint Conference on Digital Libraries, 296-305. doi:10.1145/996350.996419For the filtered dataset: 1. Kim, Jinseok (2018). Evaluating author name disambiguation for digital libraries: a case of DBLP. Scientometrics. doi:10.1007/s11192-018-2824-5 or2. Kim, Jinseok & Kim, Jenna (2018). The impact of imbalanced training data on machine learning for author name disambiguation. Scientometrics. doi: 10.1007/s11192-018-2865-9</div
Khoo Kay Kim, professor of Malaysian history : a biobibliometric study
No full textPresents an analysis of the publication productivity, authorship pattern, channels of communication, journal preference and language preference of Professor Dato' Khoo Kay Kim, Professor of Malaysian History in the University of Malaya, Kuala Lumpur. The results of this biobibliometric study indicate that he can be a role model for future Malaysian historians to emulate his various achievements especially in the field of history education
Deep learning enables fast, gentle STED microscopy
No full textSTED microscopy is widely used to image subcellular structures with super-resolution. Here, we report that denoising STED images with deep learning can mitigate photobleaching and photodamage by reducing the pixel dwell time by one or two orders of magnitude. Our method allows for efficient and robust restoration of noisy 2D and 3D STED images with multiple targets and facilitates long-term imaging of mitochondrial dynamics
Anxious, Dismal, Giddy, Aggressive: Seth Kim-Cohen interviewed by Mark Peter Wright for Ear Room.
No full textA conversation with author Seth Kim-Cohen
Kim Gordon - no icon
No full textAs cofounder of legendary rock band Sonic Youth, best-selling author, and celebrated artist, Kim Gordon is one of the most singular and influential figures of the modern era. This personally curated scrapbook is an edgy and evocative portrait of Gordon s life, art, and style. Spanning from her childhood on Californian surf beaches in the 60s and 70s to New York s downtown art and music scene in the 80s and 90s where Sonic Youth was born. Through unpublished personal photographs, magazine and newspaper clippings, fashion editorials, and advertising campaigns, interspersed with Gordon s song lyrics, writings, artworks, private objects, and ephemera, this book demonstrates how Kim Gordon has been a role model for generations of women and me
Overview of Recent Progress in Fire Suppression
No full textthis document is published in / Une version de ce document se trouve dans : Invited Keynote Lecture at the 2 nd NRIFD Symposium, Proceedings, Tokyo, Japan, July 17-19, 2002, pp. 1-13 www.nrc.ca/irc/ircpubs NRCC-45690 Title: OVERVIEW OF RECENT PROGRESS IN FIRE SUPPRESSION TECHNOLOGY Author(s): Andrew KIM Corresponding (first) author: Andrew Kim Academic degree: Ph.
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