1,721,085 research outputs found
Pedobacter pituitosus sp. nov., isolated from a waterfall
No full textA taxonomic study was carried out on a nov.l bacterial strain, designated MIC2002T, which was isolated from Wibong falls in Korea. Cells were Gram-stain-negative, aerobic, non-motile and rods, 0.3-0.5 mm wide and 4.0-5.0 mm long. The optimum temperature and pH range for growth were 25 °C and pH 6.5-7.0, respectively. Catalase and oxidase activities were positive. Flexirubin pigments were not produced. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the genus Pedobacter, with Pedobacter daechungensis as its closest relative, with a similarity of 94.4 %. It contained iso-C15 : 0, anteiso-C15 : 0, C16 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and iso-C17 : 03-OH as the major fatty acids and menaquinone MK-7 as isoprenoid quinone. The polar lipid profile of strain MIC2002Trevealed the presence of phosphatidylethanolamine and an unknown lipid. The DNA G+C content of the strain was 34.7 mol%. On the basis of the evidences presented, it was concluded that strain MIC2002T represents a nov.l sp.cies of the genus Pedobacter within the family Sphingobacteriaceae, for which the name Pedobacter pituitosus sp. nov. is proposed. The type strain is MIC2002T(=KACC 17064T=JCM 18729T).open
Niveibacterium umoris gen. nov., sp. nov., isolated from wetland freshwater
No full textA taxonomic study was carried out on a novel bacterial strain, designated MIC2059T, which was isolated from Ungok Wetland of Gochang in Korea. Cells of the isolate were found to be Gram-stain-negative, rod-shaped and motile. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the family Rhodocyclaceae, with Uliginosibacterium gangwonense as its closest relative, with a similarity of 94.8 %. It contained summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C16: 0, summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c) and C12: 0 3-OH as the major fatty acids and Q8 as the respiratory ubiquinone. The polar lipid profile of strain MIC2059T revealed the presence of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as major polar lipids. In addition, phosphatidylserine, an unidentified aminophospholipid and unidentified lipids were present in small amounts. The DNA G+C content of the strain was 65.1 mol%. On the basis of the evidence presented, it is concluded that strain MIC2059T represents a novel species of a novel genus within the family Rhodocyclaceae, for which the name Niveibacterium umoris gen. nov. sp. nov. is proposed. The type strain is MIC2059T (=KACC 17062T=JCM 18716T).open
Uliginosibacterium flavum sp. nov. isolated from an artificial lake
No full textA Gram-negative, facultative anaerobic, rod-shaped, motile by means of a polar flagellum, greenish-yellow-pigmented bacterial strain (designated strain JJ3220T) was isolated from an artificial lake in South Korea and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain JJ3220T indicated that the isolate belongs to the family Rhodocyclaceae, and that it exhibits 96.4% similarity to Uliginosibacterium paludis KBP-13T. The major cellular fatty acids of the novel strain were C14:0, C16:0, and summed feature 3 (C16:1ω6c and/or C16:1ω7c). Strain JJ3220T had flexirubin-type pigments. The DNA G+C content of the strain was 62.8%. The major respiratory quinone and major polar lipid of strain JJ3220T were ubiquinone-8 and phosphatidylethanolamine, respectively. Based on the morphological and physiological properties and biochemical evidence presented, it can be concluded that strain JJ3220T represents a novel species of the genus Uliginosibacterium. The type strain Uliginosibacterium flavum is JJ3220T (=KACC 17644T =JCM 19465T).
Psychroflexus salarius sp. nov., isolated from Gomso salt pan
No full textA taxonomic study was carried out on a nov.l bacterial strain, designated MIC1008(T), which was isolated from the Gomso salt pan, located in Buan County, Korea. Cells were Gram-stain-negative, aerobic, non-motile and moderately halophilic rods, 0.2-0.4 μm wide and 0.7-1.2 μm long. The optimum temperature and pH for growth were 25 °C and pH 6.5-8.5, respectively. Catalase and oxidase activities were positive. Carotenoid pigments were produced. Comparison of 16S rRNA gene sequences indicated that the isolate belonged to the genus Psychroflexus, with Psychroflexus halocasei WCC 4520(T) as its closest relative, with a 16S rRNA gene sequence similarity of 95.4%. The isolate contained iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH, anteiso-C15:0 and iso-C15:0 3-OH as the major fatty acids and menaquinone MK-6 as the isoprenoid quinone. The major polar lipid profile of strain MIC1008(T) revealed the presence of phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The DNA G+C content of the isolate was 32.9 mol%. On the basis of the evidences presented, it is concluded that strain MIC1008(T) represents a nov.l sp.cies of the genus Psychroflexus within the family Flavobacteriaceae, for which the name Psychroflexus http://dx.doi.org/10.1601/nm.8182 salarius sp. nov. is proposed. The type strain is MIC1008(T) ( = KACC 17063(T) = DSM 25661(T)).open
Viridibacterium curvum gen. nov., sp. nov., isolated from freshwater
No full textA Gram stain-negative, yellowish green-pigmented, facultatively anaerobic, motile, curved rod-shaped bacterium designated as strain JJ016T was isolated from an artificial lake in South Korea, and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain JJ016T indicated that the isolate belonged to the family Rhodocyclaceae and exhibited 95.0% identity to Uliginosibacterium gangwonense 5YN10-9T. The major cellular fatty acids of the novel strain were summed feature 3 (C16:1ω6c and/or C16:1ω7c), C16:0, C14:0, and summed feature 8 (C18:1ω7c and/or C18:1ω6c). The DNA G+C content of strain JJ016T was 61.9 mol%. The major respiratory quinone and major polar lipid of strain JJ016T were ubiquinone-8 and phosphatidylethanolamine, respectively. Based on the morphological and physiological properties and the biochemical evidence presented, we concluded that strain JJ016T represents a novel species of a new genus in the family Rhodocyclaceae, for which the name Viridibacterium curvum gen. nov., sp. nov. is proposed. The type strain is JJ016T (=KACC 16899T =JCM 18715T).
Gemmobacter straminiformis sp. Nov., isolated from an artificial fountain
No full textA Gram-stain-negative, non-motile and facultative anaerobic bacterium, designated CAM-8T, was isolated from an artificial fountain at Chonbuk National University, South Korea. The novel strain grew at 20-37？°C (optimum 25？°C), pH 5.5-7.0 (optimum 6.0) and with 0-2？%？NaCl (optimum 0？%). Oxidase and catalase activities were positive. The cell morphology of strain CAM-8T was atypical rods 0.6-0.8？μm in width and 4.5-6.5？μm in length, with a peaked tip and sometimes a bulb shape. CAM-8T existed as single cells, and as pairs or chains of cells. The phylogenetic analysis of 16S rRNA gene sequences indicated that strain CAM-8T clustered with Gemmobacter nectariphilus JCM 11959T and Gemmobacter megaterium JCM 18498T within the genus Gemmobacter. The DNA G+C？content of strain CAM-8T was 65.9？mol%. The respiratory quinone was ubiquinone Q-10. The major fatty acids were C18:1ω7c and/or C18？:1ω6c. The polar lipids of strain CAM-8T consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two uncharacterized phospholipids, an uncharacterized aminolipid, an uncharacterized glycolipid, an uncharacterized aminophospholipid and four uncharacterized lipids. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain CAM-8T (=KACC 19224T=JCM 31905T) is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter straminiformis sp. nov. is proposed
Flaviaesturariibacter amylovorans gen. nov., sp. nov., a starch-hydrolysing bacterium, isolated from estuarine water
No full textA novel bacterial strain, designated GCR0105T, was isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment, located in JEOLlabuk-do, South Korea. Cells of strain GCR0105T were Gram-stain-negative, non-motile and rod-shaped. Colonies of strain GCR0105T were pale yellow-pigmented on R2A agar and nutrient agar media, and were able to grow at 15？30°C (optimum 25°C) and pH6.5？8.5 (optimum pH7.5). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GCR0105T was related most closely to Flavisolibacter ginsengisoli Gsoil 643T (93.14% similarity). The polar lipid profile of strain GCR0105T comprised phosphatidylethanolamine, two unknown aminolipids, an unknown aminophospholipid and four unknown lipids. The DNA G+C content of strain GCR0105T was 42.9mol% and the respiratory quinone was MK-7.On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain GCR0105T represents a novel species in a new genus within the family Chitinophagaceae, for which the name Flaviaesturariibacter amylovorans gen. nov., sp. nov. is proposed. The type strain of Flaviaesturariibacter amylovorans is GCR0105T (=KACC 16454T=JCM 17919T).open
Identification and characterization of a cocoon degradable enzyme from the isolated strain Bacillus subtilis Bs5C
No full textA fibroin-degrading bacterium was isolated in a medium containing fibroin as a sole nitrogen source, and then identified and characterized. The strain was designated Bacillus subtilis strain Bs5C, and the culture medium containing the extracellular proteolytic enzymes was found to hydrolyze cocoon fiber. A decomposition rate of 35% was achieved with the culture medium in optimized cocoon-degrading condition. In a second round of decomposition performed on the same fibers with additional culture medium, the decomposition rate reached 45.9%. To investigate the proteolytic enzymes degrading cocoon, the enzymes was purified from culture medium using ion exchange column and identified using LC-MS/MS analysis system. As a result, it was uncovered that the cocoon degradation was due to the neutral protease NprE of the strain Bs5C. Moreover, the cocoon fibers treated with semi-purified NprE enzyme solution were clearly split and degraded, as assessed by SEM, and solubilized peptides from fibroin and sericin were detected by LC-MS/MS. In conclusion, this study is the first report that peptides could be produced from cocoon by cultured medium of B. subtilis, and the newly isolated strain Bs5C and the NprE protease from the strain Bs5C could clearly be valuable for the production of silk peptides, which have various pharmacological and industrial applications. ⓒ 2020, The Korean Society for Biotechnology and Bioengineering and Springer.
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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