1,721,049 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
암조직 대사체 분석을 통한 갑상선암 치료 표적의 탐색
No full text서론: 방사성 요오드 동위원소 치료 불응성 갑상선암의 경우 효과적인 치료 방법이 없어 환자 치료에 어려움이 있고, 승인된 분자 표적 치료 항암제의 대부분이 장기적으로 치료효과를 유지하지 못하는 실정이다. 최근 들어 암세포 특이적인 에너지대사 변화가 밝혀지고, 이러한 대사변화가 잠재적인 치료 표적이 될 수 있다는 연구결과가 보고되고 있다. 이번 연구에서 액체 색층 분석 매스 분석법을 이용하여 갑상선암 조직에서 대사체 변화를 분석하고, 이러한 변화와 갑상선암의 임상조직학적 특성과의 연관성을 알아보고자 한다.
재료 및 방법: 신선동결조직 (35개의 갑상선암 조직과 동일환자의 정상조직, 8개의 결절성 과증식 조직)과 포르말린고정 파라핀 조직 (갑상선 유두암 160개, 동일환자의 정상조직 153개)을 연구에 사용하였다. 효소의 mRNA 농도는 양적 실시간 효소 중합 반응으로 측정하였고, 면역조직화학염색을 시행하여 단백질 발현 정도를 평가하였다. shRNA 표현 렌티 바이러스 벡터를 이용해 특정 유전자를 억제하여 세포 생존 정도를 확인하였다.
결과: 갑상선 유두암 조직에서 해당경로의 대사체 농도는 3-phosphoglycerate (3PG)를 제외하고 정상조직과 결절성 과증식 조직보다 유의하게 높았다. 3PG 비율이 낮은 암 조직은 높은 조직에 비해 갑상선외 침윤이 많은 특징을 보였고 (p=0.01), BRAFV600E 유전자 변이가 있는 유두암의 경우 변이가 없는 유두암보다 3PG 농도가 낮았다 (p=0.004). PHGDH는 serine 합성의 첫 번째 단계에 작용하는 효소인데 양적 실시간 효소 중합 반응 및 면역조직화학염색으로 평가하였을 때 갑상선암조직에서 정상 갑상선 조직에 비해 발현이 유의하게 증가되어 있었고, PHGDH발현이 증가할 수록 종양크기, 경부림프절 전이율, 원격 전이율이 높았다. 그리고 BCPAP, 8505C 세포주에서 PHGDH활성을 억제하였을 때 세포 생존이 억제되었다.
결론: 이번 연구를 통해 갑상선암 조직에서도 다른 종양에서와 유사한 대사체 변화가 있음을 확인하였다. Serine 합성경로, 특히 PHGDH 발현이 갑상선 유두암에서 증가되어 있었고 이는 갑상선암의 공격성과 연관이 있었다. 또한 PHGDH 발현을 억제할 때 암세포 수명이 억제되었다. 따라서 갑상선암의 대사체 변화에 대한 연구는 새로운 치료 표적을 발견하는 밑거름이 될 것이다.
Background: The absence of effective therapy makes clinical management for radioactive iodine refractory differentiated thyroid cancer difficult. The majority of approved molecular-targeted drugs failed to induce long-lasting improvement in treatment efficacy. Recently, reprogramming of cellular energy metabolism in cancer cells have been introduced as a potential therapeutic target. We applied liquid chromatography mass spectrometry to compare the metabolite levels in thyroid cancer, and to evaluate the association of changes in metabolites of cancer and clinicopathologic characteristics.
Methods: Thirty five fresh frozen thyroid cancer tissues with matched normal tissues and 8 nodular hyperplasia (NH) tissues were used to measure metabolites. Formalin-fixed, paraffin-embedded tissues, 160 classical papillary thyroid cancers (PTC) and 153 matched normal thyroid tissue, were also collected. The mRNA expression of enzymes was evaluated by quantitative real-time PCR and the degree of protein expression was evaluated by immunohistochemistry (IHC). shRNA-expressing lentiviral vector was used to control the expression of specific genes.
Results: Metabolites levels of PTC in the glycolytic pathway were significantly higher than those of matched normal tissue and NH, except 3-phosphoglycerate (3PG) level. Cancer tissue with low 3PG ratio was associated with high frequency of extrathyroidal invasion (p=0.01). 3PG level of PTC with BRAFV600E was significantly lower than PTC with BRAFwt (p=0.004). PHGDH, an enzyme for the first step of serine synthesis, showed higher expression in PTCs than surrounding normal tissue by real time PCR (p=0.004) and IHC staining (p<0.001). Positive expression of PHGDH was significantly associated with tumor size (p=0.019) and prevalence of cervical LN metastasis (p=0.037), distant metastasis (p=0.045). Suppression of PHGDH on BCPAP and 8505C cells markedly reduced cell viability.
Conclusion: The present study identified metabolic fingerprinting of thyroid cancer tissue. Pathway for serine synthesis, especially for PHGDH expression, was activated and PHGDH expression was associated with thyroid cancer aggressiveness. Suppression of PHGDH activity markedly decreased cancer cell survival. We had shown that investigation of changes in cell metabolism may reveal novel therapeutic target(s) in thyroid cancer and that PHGDH might be one of targets to improve treatment efficacy.Docto
암조직 대사체 분석을 통한 갑상선암 치료 표적의 탐색
No full text서론: 방사성 요오드 동위원소 치료 불응성 갑상선암의 경우 효과적인 치료 방법이 없어 환자 치료에 어려움이 있고, 승인된 분자 표적 치료 항암제의 대부분이 장기적으로 치료효과를 유지하지 못하는 실정이다. 최근 들어 암세포 특이적인 에너지대사 변화가 밝혀지고, 이러한 대사변화가 잠재적인 치료 표적이 될 수 있다는 연구결과가 보고되고 있다. 이번 연구에서 액체 색층 분석 매스 분석법을 이용하여 갑상선암 조직에서 대사체 변화를 분석하고, 이러한 변화와 갑상선암의 임상조직학적 특성과의 연관성을 알아보고자 한다.
재료 및 방법: 신선동결조직 (35개의 갑상선암 조직과 동일환자의 정상조직, 8개의 결절성 과증식 조직)과 포르말린고정 파라핀 조직 (갑상선 유두암 160개, 동일환자의 정상조직 153개)을 연구에 사용하였다. 효소의 mRNA 농도는 양적 실시간 효소 중합 반응으로 측정하였고, 면역조직화학염색을 시행하여 단백질 발현 정도를 평가하였다. shRNA 표현 렌티 바이러스 벡터를 이용해 특정 유전자를 억제하여 세포 생존 정도를 확인하였다.
결과: 갑상선 유두암 조직에서 해당경로의 대사체 농도는 3-phosphoglycerate (3PG)를 제외하고 정상조직과 결절성 과증식 조직보다 유의하게 높았다. 3PG 비율이 낮은 암 조직은 높은 조직에 비해 갑상선외 침윤이 많은 특징을 보였고 (p=0.01), BRAFV600E 유전자 변이가 있는 유두암의 경우 변이가 없는 유두암보다 3PG 농도가 낮았다 (p=0.004). PHGDH는 serine 합성의 첫 번째 단계에 작용하는 효소인데 양적 실시간 효소 중합 반응 및 면역조직화학염색으로 평가하였을 때 갑상선암조직에서 정상 갑상선 조직에 비해 발현이 유의하게 증가되어 있었고, PHGDH발현이 증가할 수록 종양크기, 경부림프절 전이율, 원격 전이율이 높았다. 그리고 BCPAP, 8505C 세포주에서 PHGDH활성을 억제하였을 때 세포 생존이 억제되었다.
결론: 이번 연구를 통해 갑상선암 조직에서도 다른 종양에서와 유사한 대사체 변화가 있음을 확인하였다. Serine 합성경로, 특히 PHGDH 발현이 갑상선 유두암에서 증가되어 있었고 이는 갑상선암의 공격성과 연관이 있었다. 또한 PHGDH 발현을 억제할 때 암세포 수명이 억제되었다. 따라서 갑상선암의 대사체 변화에 대한 연구는 새로운 치료 표적을 발견하는 밑거름이 될 것이다.
Background: The absence of effective therapy makes clinical management for radioactive iodine refractory differentiated thyroid cancer difficult. The majority of approved molecular-targeted drugs failed to induce long-lasting improvement in treatment efficacy. Recently, reprogramming of cellular energy metabolism in cancer cells have been introduced as a potential therapeutic target. We applied liquid chromatography mass spectrometry to compare the metabolite levels in thyroid cancer, and to evaluate the association of changes in metabolites of cancer and clinicopathologic characteristics.
Methods: Thirty five fresh frozen thyroid cancer tissues with matched normal tissues and 8 nodular hyperplasia (NH) tissues were used to measure metabolites. Formalin-fixed, paraffin-embedded tissues, 160 classical papillary thyroid cancers (PTC) and 153 matched normal thyroid tissue, were also collected. The mRNA expression of enzymes was evaluated by quantitative real-time PCR and the degree of protein expression was evaluated by immunohistochemistry (IHC). shRNA-expressing lentiviral vector was used to control the expression of specific genes.
Results: Metabolites levels of PTC in the glycolytic pathway were significantly higher than those of matched normal tissue and NH, except 3-phosphoglycerate (3PG) level. Cancer tissue with low 3PG ratio was associated with high frequency of extrathyroidal invasion (p=0.01). 3PG level of PTC with BRAFV600E was significantly lower than PTC with BRAFwt (p=0.004). PHGDH, an enzyme for the first step of serine synthesis, showed higher expression in PTCs than surrounding normal tissue by real time PCR (p=0.004) and IHC staining (p<0.001). Positive expression of PHGDH was significantly associated with tumor size (p=0.019) and prevalence of cervical LN metastasis (p=0.037), distant metastasis (p=0.045). Suppression of PHGDH on BCPAP and 8505C cells markedly reduced cell viability.
Conclusion: The present study identified metabolic fingerprinting of thyroid cancer tissue. Pathway for serine synthesis, especially for PHGDH expression, was activated and PHGDH expression was associated with thyroid cancer aggressiveness. Suppression of PHGDH activity markedly decreased cancer cell survival. We had shown that investigation of changes in cell metabolism may reveal novel therapeutic target(s) in thyroid cancer and that PHGDH might be one of targets to improve treatment efficacy.Docto
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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