1,721,035 research outputs found
Stabilization of quinonoid intermediate E-Q by Glu32 of D-amino acid transaminase
No full textThe stable anchorage of pyridoxal 5'-phosphate (PLP) in the active site Of D-amino acid transaminase (D-AT) is crucial for the enzyme catalysis. The three-dimensional structure Of D-AT revealed that Glu32 is one of the active site groups that may play a role in PLP binding. To prove the role of Glu32 in PLP stability, we firstly checked the rate of the potential rate-limiting step. The kinetic analysis showed that the rate of the alpha-deprotonation step reduced to 26-folds in E32A mutant enzyme. Spectral analyses of the reaction Of D-AT with D-serine revealed that the E32A mutant enzyme failed to stabilize the key enzyme-substrate intermediate, namely a quinonoid intermediate (E-Q). Finally, analysis of circular dichroism (CD) on the wild-type and E32A mutant enzymes showed that the optical activity of PLP in the enzyme active site was lost by the removal of the carboxylic group, proving that Glu32 is indeed involved in the cofactor anchorage. The results suggested that the electrostatic interaction network through the groups from PLP, Glu32, His47, and Arg50, which was observed from the three-dimensional structure of the enzyme, plays a crucial role in the stable anchorage of the cofactor to give necessary torsion to the plane of the cofactor-substrate complex
A report of 35 unrecorded bacterial species belonging to the classes Alphaproteobacteria and Betaproteobacteria in Korea
No full textJung, Hye Su, Yoon, Jung-Hoon, Joh, Kiseong, Seong, Chi-Nam, Kim, Won-Yong, Im, Wan-Taek, Kim, Myung-Kyum, Cha, Chang-Jun, Kim, Seung-Bum, Jeon, Che-Ok (2021): A report of 35 unrecorded bacterial species belonging to the classes Alphaproteobacteria and Betaproteobacteria in Korea. Journal of Species Research 10 (1): 12-22, DOI: 10.12651/JSR.2021.10.1.01
Report on 31 unrecorded bacterial species in Korea that belong to the phylum Actinobacteria
No full textChoi, Jung-Hye, Cha, Ju-Hee, Bae, Jin-Woo, Cho, Jang-Cheon, Chun, Jongsik, Im, Wan-Taek, Jahng, Kwang Yeop, Jeon, Che Ok, Joh, Kiseong, Kim, Seung Bum, Seong, Chi Nam, Yoon, Jung-Hoon, Cha, Chang-Jun (2016): Report on 31 unrecorded bacterial species in Korea that belong to the phylum Actinobacteria. Journal of Species Research 5 (1): 1-13, DOI: 10.12651/JSR.2016.5.1.001, URL: http://dx.doi.org/10.12651/jsr.2016.5.1.00
Streptomyces atratus MA
No full textDescription of <i>Streptomyces atratus</i> MA 10 <p>Cells are Gram-staining-negative, non-flagellated and rod­shaped. Colonies are circle and yellow­colored after 2 days on MA at 25° C. Strain MA10 (= NIBRBA00 00113970) was isolated from a forest soil sample, Gwanak Mountain, Seoul, Korea.</p>Published as part of <i>Choi, Jung-Hye, Cha, Ju-Hee, Bae, Jin-Woo, Cho, Jang-Cheon, Chun, Jongsik, Im, Wan-Taek, Jahng, Kwang Yeop, Jeon, Che Ok, Joh, Kiseong, Kim, Seung Bum, Seong, Chi Nam, Yoon, Jung-Hoon & Cha, Chang-Jun, 2016, Report on 31 unrecorded bacterial species in Korea that belong to the phylum Actinobacteria, pp. 1-13 in Journal of Species Research 5 (1)</i> on page 11, DOI: 10.12651/JSR.2016.5.1.001, <a href="http://zenodo.org/record/8120360">http://zenodo.org/record/8120360</a>
Ramlibacter henchirensis BT
No full textDescription of Ramlibacter henchirensis BT 69 Cells are Gram-staining-negative, non-flagellated, and rod shaped. Colonies are circular, smooth, and white colored after 3 days of incubation at 25℃ on R2A. Positive for gelatinase activity. Negative for nitrate reduction, indole production, glucose fermentation, esculin hydrolysis, and arginine dihydrolase, urease, and β -galactosidase activities. Does not utilize D- glucose, L- arabinose, D- mannose, D- mannitol, N -acetylglucosamine, D- maltose, potassium gluconate, capric acid, adipic acid, malic acid, trisodium citrate, and phenylacetic acid. Strain BT69 (= NIBRBAC000502987) was isolated from a soil sample, Pyeongchang, Korea.Published as part of Jung, Hye Su, Yoon, Jung-Hoon, Joh, Kiseong, Seong, Chi-Nam, Kim, Won-Yong, Im, Wan-Taek, Kim, Myung-Kyum, Cha, Chang-Jun, Kim, Seung-Bum & Jeon, Che-Ok, 2021, A report of 35 unrecorded bacterial species belonging to the classes Alphaproteobacteria and Betaproteobacteria in Korea, pp. 12-22 in Journal of Species Research 10 (1) on page 21, DOI: 10.12651/JSR.2021.10.1.012, http://zenodo.org/record/812000
Fig. 2. A in A report of 35 unrecorded bacterial species belonging to the classes Alphaproteobacteria and Betaproteobacteria in Korea
No full textFig. 2. A neighbor-joining phylogenetic tree, based on 16S rRNA gene sequences, showing the phylogenetic relationships between the strains isolated in this study and their relatives in the orders Caulobacterales, Rhizobiales, Rhodobacterales, Rhodospirillales, and Sphingomonadales of the class of Alphaproteobacteria. Bootstrap values (>70%) are shown above nodes. Scale bar: 0.02 changes per nucleotide.Published as part of Jung, Hye Su, Yoon, Jung-Hoon, Joh, Kiseong, Seong, Chi-Nam, Kim, Won-Yong, Im, Wan-Taek, Kim, Myung-Kyum, Cha, Chang-Jun, Kim, Seung-Bum & Jeon, Che-Ok, 2021, A report of 35 unrecorded bacterial species belonging to the classes Alphaproteobacteria and Betaproteobacteria in Korea, pp. 12-22 in Journal of Species Research 10 (1) on page 17, DOI: 10.12651/JSR.2021.10.1.012, http://zenodo.org/record/812000
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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