976 research outputs found
Maynard Jackson, Valerie, and Their Children, 1988
No full textMayor Jackson with his family. Front row: Maynard III "Buzzy" and Brooke. Back Row: Valerie-Amanda, Valerie, Maynard Jr., Elizabeth, and Alexandra
Maynard Jackson's Speech to Vietnam Veterans, June 7, 1973
No full textA speech given by Maynard Jackson at the Vietnam Veterans Conference
Maynard Holbrook Jackson Sr., circa 1940
No full textPictured here in his senior class photo, the Reverend Maynard Holbrook Jackson, Sr. graduated from Morehouse College and then studied at the Garrett School of Divinity of Northwestern University. His father, A. S. Jackson, was a member of Atlanta University's charter class
Bill Cosby, Maynard Jackson, and Sidney Poitier, circa 1975
No full textMaynard Jackson with Bill Cosby (left) and Sidney Poitier (right) while in Atlanta to film "Let's Do It Again", circa 1975. While maintaining the common touch, Jackson benefitted from the support of some star entertainers over the years
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Optimized protocol for antibody yeast display
No full textYeast display is an established technology used to discover proteins that have increased binding affinities to their targets. There are many steps within the process that affect the final binding affinity between proteins. The Maynard Lab regularly utilizes display technologies to discover antibodies that bind strongly to targets of interest prior to antibody engineering, in vitro testing, and in vivo studies. For the purpose of efficiency and standardization, the goal of this work is to develop a protocol associated with yeast display for discovery of potent antibodies. In this work, the variable domain is expressed on the surface of yeast cells, in which each cell has a unique heavy chain genotype and matching phenotype based on error-prone mutagenesis. After artificially producing millions of yeast cells with different heavy chain regions, the cells are sorted based on their affinity towards a receptor. DNA sequences that encode for successful binders are used to recombinantly clone, express, and characterize antibodies as leading candidates with increased binding affinity to targets. This revised protocol is lab-specific, wherein details about execution of experiments are contextual to the work done in the Maynard Lab. Every bullet-point and step in this report is a lesson learned from significant testing and troubleshooting.Chemical Engineerin
Rural dress in Australia [Volume 7, Part 3]
No full text<b>The Clothing Habits of Rural Australia</b>\ud
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Despite the common perception that dress has not been a preoccupation of Australians, who prefer practical and comfortable clothes, in fact, the history of Australia reveals a strong preoccupation with clothing from the outset. Established as a penal colony by England, the early settlers were far from home with few material comforts or familiar accoutrements of everyday life. The climate, landscape, vegetation, fauna, and flora were exotic, harsh, and alien. Immediate priorities were establishing supplies of food and creating habitable shelter—clothing the prisoners and settlers ran a close third
Chapter 18 of the General Theory “Further Analysed”: The Theory of Economics as A Method
No full textIn 1987, Greenwald and Stiglitz accused Keynes’s summary of the General Theory in chapter 18 of relying upon “neoclassical and Marshallian tools”. A number of contributions have on the contrary emphasized the methodological importance of this chapter, which this paper revisits in the light of A Treatise on Probability. It thereby shows that the notions of cause and dependence used to discuss the relationships between independent and dependent variables of the General Theory are related to the concept of “independence for knowledge”, which concerns logical connections between arguments rather than material connections between events. We demonstrate that such logical connections established in chapter 18 are rediscussed in chapters 19-21, where Keynes allows for probable repercussions between the factors and removes the simplifying assumptions previously introduced. After stressing the methodological continuity this method provides with the analysis of credit cycles in A Treatise on Money, we argue that chapter 18 is an indispensable tool to decode the internal text structure of the General Theory. We thus characterize the latter as a vademecum to the complex economic world, the author providing an analytical method allowing – and requiring – the readers to emulate his efforts to grasp the complexity and interdependence of the economic material.John Maynard Keynes, The General Theory, complexity, economic methodology
Maynard Participation in Alaska Forum on the Environment Panel Discussion on Increasing Input to the US National Climate Assessment (NCA) and the Intergovernmental Panel on Climate Change (IPCC) Processes from Alaska, with Emphasis on Indigenous Peoples Issues
No full textDr. Nancy Maynard was invited by the Alaska Forum on the Environment to participate in a Panel Discussion to discuss (1) background about what the US NCA and International IPCC assessments are, (2) the impact the assessments have on policy-making, (3) the process for participation in both assessments, (4) how we can increase participation by Indigenous Peoples such as Native Americans and Alaska Natives, (5) How we can increase historical and current impacts input from Native communities through stories, oral history, "grey" literature, etc. The session will be chaired by Dr. Bull Bennett, a cochair of the US NCA's chapter on "Native and Tribal Lands and Resources" and Dr. Maynard is the other co-chair of that chapter and they will discuss the latest activities under the NCA process relevant to Native Americans and Alaska Natives. Dr. Maynard is also a Lead Author of the "Polar Regions" chapter of the IPCC WG2 (5th Assessment) and she will describes some of the latest approaches by the IPCC to entrain more Indigenous peoples into the IPCC process
Antibodies Recognizing Protective Pertussis Toxin Epitopes Are Preferentially Elicited by Natural Infection versus Acellular Immunization
No full textDespite more than 50 years of vaccination, disease caused by the bacterium Bordetella pertussis persists, with rates increasing in industrialized countries over the past decade. This rise may be attributed to several factors, including increased surveillance, emergence of vaccine escape variants, waning immunity in adults, and the introduction of acellular subunit vaccines, which include chemically detoxified pertussis toxin (PTd). Two potently protective epitopes on pertussis toxin (PTx) are recognized by the monoclonal antibodies 1B7 and 11E6, which inhibit catalytic and cell-binding activities, respectively. In order to determine whether the PTx exposure route affects antibody responses to these epitopes, we analyzed sera from 30 adults with confirmed pertussis exposure and from 30 recently vaccinated adults for specific anti-PTx antibody responses and in vitro CHO cell neutralization titers. While overall titers against PTx and the genetically detoxified variant, PTg, containing the R9K and E129G substitutions, were similar in the two groups, titers against specific epitopes depended on the exposure route. Natural infection resulted in significantly higher titers of anti-PTx-subunit 1, 1B7-like, and 11E6-like antibodies, while acellular vaccination resulted in significantly higher titers of anti-bodies recognizing PTd. We also observed a correlation between in vitro protection and the presence of 1B7-like and 11E6-like antibodies. Notably, chemical detoxification, as opposed to genetic inactivation, alters the PTx tertiary and quaternary structure, thereby affecting conformational epitopes and recognition of PTx by 1B7 an
A real-time assay for CpG-specific cytosine-C5 methyltransferase activity
No full textA real-time assay for CpG-specific cytosine-C5 methyltransferase activity has been developed. The assay applies a break light oligonucleotide in which the methylation of an unmethylated 5?-CG-3? site is enzymatically coupled to the development of a fluorescent signal. This sensitive assay can measure rates of DNA methylation down to 0.34 ± 0.06 fmol/s. The assay is reproducible, with a coefficient of variation over six independent measurements of 4.5%. Product concentration was accurately measured from fluorescence signals using a linear calibration curve, which achieved a goodness of fit (R2) above 0.98. The oligonucleotide substrate contains three C5-methylated cytosine residues and one unmethylated 5?-CG-3? site. Methylation yields an oligonucleotide containing the optimal substrate for the restriction enzyme GlaI. Cleavage of the fully methylated oligonucleotide leads to separation of fluorophore from quencher, giving a proportional increase in fluorescence. This method has been used to assay activity of DNMT1, the principle maintenance methyltransferase in human cells, and for the kinetic characterization of the bacterial cytosine-C5 methyltransferase M.SssI. The assay has been shown to be suitable for the real-time monitoring of DNMT1 activity in a high-throughput format, with low background signal and the ability to obtain linear rates of methylation over long periods, making this a promising method of high-throughput screening for inhibitors. <br/
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