1,720,961 research outputs found
Cyt D does not induce MET in normal fibroblasts.
No full text<p>(A) HT-1080 and Hs27 cells were treated overnight with various concentrations of Cyt D (0, 50, 100, 200 μM) and 1% DMSO as a vehicle control and cell lysates blotted for E-cadherin, N-cadherin, vimentin and β-actin. (B) For migration assays, cells plated on 8 μM cell inserts for 4–6 hrs were treated with the indicated concentrations of Cyt D for 12 hrs and the number of cell migrating through the filter counted. (C) To assess cytoxicity of Cyt D, cells were plated overnight and then treated with the indicated concentrations of Cyt D for 10 hrs. WST-1 reagent was added to each well and after two hours absorbance was read at 450 nm. *<i>p</i>>0.05, **<i>p</i>>0.01 and ***<i>p</i>>0.001; DMSO treated cells relative to untreated cells; Cyt D treated cells relative to DMSO treated cells.</p
Cyt D treatment induces nuclear-cytoplasmic distribution of SNAI 1 and SMAD1/2/3.
No full text<p>(A) Du145, MDA-MB-231, MDA-MB-435, HT-1080, U251 and U87 cells were untreated (Control) or treated with 100 μM Cyt D (Cyt D treated) for 12 h and immunofluorescently labelled for Smad1/2/3 and SNAI 1 as well as Hoechst 33342 for nuclear staining. Representative images for MDA-MB-231 cells are shown. (B) Cells were scored for nuclear distribution of Smad1/2/3 and SNAI 1 in control and Cyt D treated cells. Results are presented as percentage of total cell showing nuclear distribution for these two proteins. Scale: 10 μm; **, <i>p</i><0.01, * <i>p</i><0.05; relative to untreated control for each cell line.</p
Concentration-dependent induction of E-cadherin protein and mRNA by Cyt D.
No full text<p>(A) Du145, MDA-MB-231, MDA-MB-435, HT-1080, U251 and U87 cells were treated overnight with various concentration of Cyt D (0, 10, 50, 100, 200 μM) and cell lysates blotted for E-cadherin, N-cadherin, vimentin and β-actin. (B) Real-time PCR for E-cadherin mRNA was performed on total RNA isolated from the Du145, MDA-MB-231, MDA-MB-435, HT-1080, U251 and U87 cells treated with different concentrations of Cyt D. Cyt D treatment increased E-cadherin mRNA expression in all cells except for HT1080 and MDA-231 where there was no or minimal expression. **, <i>p</i><0.01, * <i>p</i><0.05; relative to control (untreated) cells.</p
Actin polymerization regulates E-cadherin expression and RhoA GTP levels in MCF-7 cells.
No full text<p>MCF-7 cells were treated with 100 μM Cyt D for 12 h (A) or 0–400 nM Jasplakinolide (JP) for 12 h. (B) and lysates were blotted for E-cadherin and β-actin. (C) RhoA GTP and total RhoA level were determined in MCF-7 cells treated with 100 μM Cyt D for 12 h. ***<i>p</i>>0.001; relative to control. (D) MCF-7 cells were transfected with dominant-negative (DN), wild type (WT) and dominant-active (DA) RhoA for 48 h after which cell lysates were probed for c-Myc, E-cadherin and β-actin. (E) Untransfected MCF-7 cells or MCF-7 cells transfected with dominant-active RhoA active were treated with 100 μM Cyt D for 12 h and cell lysates probed for c-Myc, E-cadherin and β-actin.</p
Time-dependent induction of E-cadherin protein and mRNA by Cyt D.
No full text<p>(A) Du145, MDA-MB-231, MDA-MB-435, HT-1080, U251 and U87 cells were treated with Cyt D for the indicated times and cell lysates blotted for E-cadherin, N-cadherin, vimentin and β-actin. (B) Real-time PCR for E-cadherin mRNA was performed on total RNA isolated from the Du145, MDA-MB-231, MDA-MB-435, HT-1080, U251 and U87 cells treated at different time interval with Cyt D. Cyt D treatment increased E-cadherin mRNA expression level in all cells except for HT1080 and MDA-231 where there was no or minimal expression. **, <i>p</i><0.01, * <i>p</i><0.05; relative to time 0.</p
Disruption of the actin cytoskeleton with Cyt D reduces cell size and F-actin content and increases E-cadherin expression in metastatic cancer cells.
No full text<p>(A) Du145, MDA-231, MDA-435, HT1080, U251 and U87 cells plated for 24 h were untreated (Control) or treated with 100 μM Cyt D (Cyt D treated) for 12 h, fixed and immunofluorescently labeled for E-cadherin (green) and F-actin (red). Scale: 10 μm. (B) Du145, MDA-MB-231, MDA-MB-435, HT-1080, U251 and U87 cells were treated with different concentrations of Cyt D (0, 10, 50, 100, 200 μM) and mean size and F-actin content of the cells were determined using the Morphology Explorer Bioapplication Software of a Cellomics ArrayScan VTI HCS Reader. **<i>p</i>>0.01 and * <i>p</i><0.05; relative to control (untreated) cells.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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