10 research outputs found
Pengembangan Budaya Organisasi di Lembaga Peradilan
No full textThis research concerns with organizational culture in a judiciary institution, with a case study at the Secretariat General and Clerk’s Office of the Indonesian Constitutional Court. The purpose of this research is to learn about the organizational culture growing in the institution, identify the appropriate culture and formulate choices of possible development. This research used qualitative approach. The data were collected through the method of observation, survey, and focus group discussion. The result of this research shows that in the dimension of external adaptation tasks, the culture developed in the Secretariat General and Clerk’s Office of the Indonesian Constitutional Court are the modern, rational, immediate, precise, and result-oriented culture. The development of organizational culture in the Secretariat General and Clerk’s Office of the Indonesian Constitutional Court is oriented to the shaping of impartial, integrated, immediate, precise, and professional culture
Adapting authoritarianism: institutions and co-optation in Egypt and Syria
No full textThis PhD thesis compares Egypt and Syria’s authoritarian political systems. While the tendency in social science political research treats Egypt and Syria as similarly authoritarian, this research emphasizes differences between the two systems with special reference to institutions and co-optation. Rather than reducibly understanding Egypt and Syria as sharing similar histories, institutional arrangements, or ascribing to the oft-repeated convention that “Syria is Egypt but 10 years behind,” this thesis focuses on how events and individual histories shaped each states current institutional strengthens and weaknesses. Specifically, it explains the how varying institutional politicization or de-politicization affects each state’s capabilities for co-opting elite and non-elite individuals.
Beginning with a theoretical framework that considers the limited utility of democratization and transition theoretical approaches, the work underscores the persistence and durability of authoritarianism. Chapter two details the politicized institutional divergence between Egypt and Syria that began in the 1970s. Chapter three and four examines how institutional politicization or de-politicization affects elite and non-elite individual co-optation in Egypt and Syria. Chapter five discusses the study’s general conclusions and theoretical implications.
This thesis’s argument is that Egypt and Syria co-opt elites and non-elites differently because of the varying degrees of institutional politicization in each governance system. Rather than view one country as more politically developed than the other, this work argues that Syria’s political institutions are more politicized than their Egyptian counterparts. Syria’s political arena is, thus, described as politicized-patrimonialism. Syria’s politicized-patrimonial arena produces uneven co-optation of elites and non-elites as they are diffused through competing institutions. Conversely, the Egyptian political arena remains highly personalized as weak institutions and individuals are manipulated and molded according to the president’s ruling clique. This is referred to as personalized-patrimonialism. As a consequence, Egypt’s political establishment demonstrates more flexibility in ad hoc altering and adapting its arena depending on the emergence of crises.
This study’s theoretical implications suggest that, contrary to modernization and democratization theory’s adage that institutions lead to a political development, politicized institutions within a patrimonial order actually hinder regime adaptation because consensus is harder to achieve and maintain. It is within this context that Egypt’s de-politicized institutional framework advantages its top political elite. In this reading of Egyptian and Syrian politics, Egypt’s personalized political arena is more adaptable than Syria’s. These conclusions do not indicate that political reform is a process underway in either state
Mapping Knowledge Domains of Global Digital Library Research Output: A Scientometric Assessment
No full textDigital Libraries have emerged as an essential component of worldwide information infrastructure, adopting the latest Information and Communication Technology (ICT) to promote an organizational structure that encourages communication and resource sharing between academicians and researchers across the countries. The study explored global digital library literature that has been carried out in the years 2007-2021 based on various scientometric indicators such as publication output, prolific author, collaboration network by author, productive institution, and country. The key term "digital library" was used in our search strategy from the Web of Science. The data was saved and imported into R Studio and VOS viewer for analysis and illustration of our data. We accessed 5855 records retrieved from the Web of Science. The United States had the most documents published, followed by China and England. The journals of Electronic Library were ranked first. The University of California System was the top organization with many scientific publications with 5549 citations. This research gives scholars and practitioners a thorough overview of the key research issues and trends in digital library research worldwide. Digital libraries worldwide have impacted the research society. It is an extension of a physical library into an electronic library. This study is very significant in the current scenario to know the global growth rate of scientific literature
Programmable Insight: A Computational Methodology to Explore Online News Use of Frames
Get PDFabstract: The Internet is a major source of online news content. Online news is a form of large-scale narrative text with rich, complex contents that embed deep meanings (facts, strategic communication frames, and biases) for shaping and transitioning standards, values, attitudes, and beliefs of the masses. Currently, this body of narrative text remains untapped due—in large part—to human limitations. The human ability to comprehend rich text and extract hidden meanings is far superior to known computational algorithms but remains unscalable. In this research, computational treatment is given to online news framing for exposing a deeper level of expressivity coined “double subjectivity” as characterized by its cumulative amplification effects. A visual language is offered for extracting spatial and temporal dynamics of double subjectivity that may give insight into social influence about critical issues, such as environmental, economic, or political discourse. This research offers benefits of 1) scalability for processing hidden meanings in big data and 2) visibility of the entire network dynamics over time and space to give users insight into the current status and future trends of mass communication.Dissertation/ThesisDoctoral Dissertation Computer Science 201
FOXM1 induces a global methylation signature that mimics the cancer epigenome in head and neck squamous cell carcinoma.
No full textPMCID: PMC3312909This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.The oncogene FOXM1 has been implicated in all major types of human cancer. We recently showed that aberrant FOXM1 expression causes stem cell compartment expansion resulting in the initiation of hyperplasia. We have previously shown that FOXM1 regulates HELLS, a SNF2/helicase involved in DNA methylation, implicating FOXM1 in epigenetic regulation. Here, we have demonstrated using primary normal human oral keratinocytes (NOK) that upregulation of FOXM1 suppressed the tumour suppressor gene p16(INK4A) (CDKN2A) through promoter hypermethylation. Knockdown of HELLS using siRNA re-activated the mRNA expression of p16(INK4A) and concomitant downregulation of two DNA methyltransferases DNMT1 and DNMT3B. The dose-dependent upregulation of endogenous FOXM1 (isoform B) expression during tumour progression across a panel of normal primary NOK strains (n = 8), dysplasias (n = 5) and head and neck squamous cell carcinoma (HNSCC) cell lines (n = 11) correlated positively with endogenous expressions of HELLS, BMI1, DNMT1 and DNMT3B and negatively with p16(INK4A) and involucrin. Bisulfite modification and methylation-specific promoter analysis using absolute quantitative PCR (MS-qPCR) showed that upregulation of FOXM1 significantly induced p16(INK4A) promoter hypermethylation (10-fold, P<0.05) in primary NOK cells. Using a non-bias genome-wide promoter methylation microarray profiling method, we revealed that aberrant FOXM1 expression in primary NOK induced a global hypomethylation pattern similar to that found in an HNSCC (SCC15) cell line. Following validation experiments using absolute qPCR, we have identified a set of differentially methylated genes, found to be inversely correlated with in vivo mRNA expression levels of clinical HNSCC tumour biopsy samples. This study provided the first evidence, using primary normal human cells and tumour tissues, that aberrant upregulation of FOXM1 orchestrated a DNA methylation signature that mimics the cancer methylome landscape, from which we have identified a unique FOXM1-induced epigenetic signature which may have clinical translational potentials as biomarkers for early cancer screening, diagnostic and/or therapeutic interventions
The Urgency of Modifying Regulations for the Safety of General Election Organizers in 2024
No full textGeneral elections in Indonesia have been held 12 times, namely in 1955, 1971, 1977, 1982, 1987, 1992, 1997, 1999, 2004, 2009, 2014 and 2019. And, the general election simultaneously and costing not a few lives occurred in 2019. Basically, the simultaneous general elections held in 2019 had many irregularities, especially the large number of members of ad hoc election organizers who fell ill and died. This happened in almost every region. Does the 2019 general election have anything to do with the increased workload of ad hoc election organizers due to the simultaneous implementation? Type of research uses normative legal research methods. It is called so, because normative research focuses on prescriptive research, using secondary data such as laws and regulations, legal theories, legal principles, and scientific works of legal scholars. The problems that occurred in the 2019 general election can be anticipated by making adequate regulatory modifications in order to create a rational, humane authority, and better election management than the previous period. There needs to be a guarantee of the quality of direct, general, free, secret, honest and fair elections. The implementation of simultaneous elections has provided a heavy workload, long implementation time and very tiring manual vote counting. Members of the voting and counting organizing group need to be protected by making some kind of regulation in their favor.___
References
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Indra, M. (2011). Dinamika Hukum Tata Negara Indonesia, Bandung: PT Refika Adiatma.
Ramadhanil, F., Pratama, H. M., Salabi, N. A., & Sadikin, U. H., (2019). Evaluasi Pemilu Serentak 2019: Dari Sistem Pemilu ke Manajemen Penyelengaraan Pemilu, Jakarta: Perkumpulan untuk Pemilu dan Demokrasi (Perludem).
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Suyanto. (2023). Metode Penelitian Hukum Pengantar Penelitian Normatif, Empiris dan Gabungan, Gresik: Unigres Press.
Journal articles:
Abbas, A., & Dompak, T. (2020). Dampak Demokrasi terhadap Kesejahteraan (Studi Kasus di Indonesia). Dialektika Publik, 5(1), 43-49. https://doi.org/10.33884/dialektikapublik.v5i1.2417
Carney, G. (2015). Comparative Approaches to Statutory Interpretation in Civil Law and Common Law Jurisdictions. Statute Law Review, 36(1), 46-58. https://doi.org/10.1093/slr/hmu019
Dwianto, A., & Makhali, I. (2024). Analisis Penerapan Asas Langsung, Umum, Bebas, Rahasia, Jujur, dan Adil terhadap Pemilihan Wakil Bupati Tulungagung Sisa Masa Jabatan 2018-2023. Mizan: Jurnal Ilmu Hukum, 13(1), 29-44. ttps://doi.org/10.32503/mizan.v13i1.5329
Hariati, S., Nasution, M. A., & Warjio, W. (2021). Analisis Faktor Eksternal yang Mempengaruhi Beban Kerja Kelompok Penyelenggara Pemungutan Suara pada Pemilihan Umum 2019. PERSPEKTIF, 10(2), 467-474. https://doi.org/10.31289/perspektif.v10i2.4616
Hofi, M. A., & Wicaksono, T. (2023). Menjaga Eksistensi Kedaulatan Rakyat Dalam Bidang Politik, Hukum, dan Ekonomi. Hakim: Jurnal Ilmu Hukum dan Sosial, 1(3), 141-153. https://doi.org/10.51903/hakim.v1i3.1235
Mustawa, M., Hamid, A. H., & Purwanda, S. (2022). Refund of State Financial Losses in Realizing the Welfare State of Law. Amsir Law Journal, 4(1), 51-61. https://doi.org/10.36746/alj.v4i1.125
Purwanda, S., Ambarwati, A., Darmawati, D., & Prayudi, P. (2024). Haluan Kesejahteraan Sosial Dalam Diskursus Teori-Teori Keadilan. Dinamika Hukum, 25(1), 152-161. https://doi.org/10.35315/dh.v25i1.9819
Putri, N. H., Laia, A., & Laia, B. (2023). Sistem Proporsional Pemilihan Umum Dalam Perspektif Politik Hukum. Jurnal Panah Keadilan, 2(2), 66-80. https://doi.org/10.57094/jpk.v2i2.1006
Ratama, R., Situmorang, T. P., & Ginting, B. (2023). Analisis Kinerja Kelompok Penyelenggara Pemungutan Suara (KPPS) dalam Pemilihan Umum Tahun 2019 di Kabupaten Tapanuli Utara. PERSPEKTIF, 12(3), 1030-1042. https://doi.org/10.31289/perspektif.v12i3.9731
Ridlwan, Z. (2011). Negara Hukum Indonesia Kebalikan Nachtwachterstaat. Fiat Justisia: Jurnal Ilmu Hukum, 5(2), 141-152. https://doi.org/10.25041/fiatjustisia.v5no2.56
Sumanto, D., & Nggilu, S. (2020). Kedudukan Peraturan Komisi Pemilihan Umum (PKPU) Dalam Tata Susunan Peraturan Perundang-undangan Republik Indonesia. Datuk Sulaiman Law Review (DaLRev), 1(1), 27-38. https://doi.org/10.24256/dalrev.v1i1.1594
Tarina, U., Febrian, M. R., Prihastuti, I. C., & Nurcahya, W. F. (2024). Efektivitas Penyerapan Anggaran Belanja Negara Dalam Pemilihan Umum. Journal of Law, Administration, and Social Science, 4(5), 785-797. https://doi.org/10.54957/jolas.v4i5.885
Wijaya, S., Amiruddin, S., & Budiati, A. (2023). Strategi Komisi Pemilihan Umum Kabupaten Pandeglang untuk Meningkatkan Partisipasi Pemilih Dalam Pilkada Serentak di Kabupaten Pandeglang Tahun 2020. Jurnal Cahaya Mandalika, 4(3), 335-349. https://doi.org/10.36312/jcm.v4i3.178
Caymanianness, history, culture, tradition, and globalisation : assessing the dynamic interplay between modern and traditional(ist) thought in the Cayman Islands
Get PDFThe research undertaken for this largely qualitative dissertation draws on newspaper articles, oral
histories, historical documentation, open-ended interviews, and to a lesser extent, questionnaires, in
the effort to ultimately confirm the extent to which the benefitting forces of globalization have
fractured any existing traditional-historical cultural body of knowledge and expression among the
Caymanian people. Indeed, by 2009 some Caymanians had long been verbally denouncing the social
and cultural ills of globalization – inclusive of multiculturalism – on their so-called traditional,
unassuming way of life, some of them clamoring for an extensive purge of the many foreign
nationals in “their” Cayman Islands. Yet, other Caymanians have become somewhat invested in the
idea of multicultural “oneness” ostensibly for the sake of peaceful coexistence, harmony and
prosperity as these work towards the promotion of a global, borderless cultural awareness.
This dissertation relies on theoretical frames centred both on the discrete natures of, and the
dualistic struggle between, these two opposing ideological-cultural forces. That this struggle is taking
place in the present age, I anticipate the ways in which more modern understandings, which are
potentially open to liberating subjectivities, must clash with “historical”, xenophobic and
nationalistic viewpoints, viewpoints which have constantly proven contradictory given their
adherents’ complacent acceptance of, and participation in, a localised economic prosperity
substantively dependent on foreign input. Thus in aggregate terms, this dissertation pinpoints the
various effects of an evolving scheme of values and counter-values on an ideologically torn
Caymanianness whose contradictory traditional half is especially fighting for its “cultural purity” in
an era where its ‘reinvented’ logic is being more and more regarded as anachronistic and somewhat
irrational
ANALISIS SIMPLISITAS DAN INTERAKTIVITAS DALAM MEMBANGUN KEPERCAYAAN MEREK : (Studi pada Pelanggan Telepon Seluler di DKI Jakarta dan Jawa Barat)
Get PDFABSTRACT
Tjahjono Djatmiko (1308209) “Analysis of Simplicity and Interactivity in developing Brand Trust (Study on Cell Phone Subscribers in Jakarta and West Java” Doctoral Dissertation Graduate School of Management Studies University of Indonesia, under guidance of Prof. Dr. Hj. Ratih Hurriyati, MP and Dr. Lili Adi Wibowo, S. Sos., S.Pd., MM.
Cellular telecommunications service industry in Indonesia is growing very rapidly. There are three major cellular telecom operators in Indonesia that dominating this service. The number of customers who served by each cellular operators is always fluctuating every year. The customer loyalty of each operators are not the same. The unstable loyalty of the customer is giving an indication that the service quality of the operators sometimes not meet the expectation of the customer that finally become the factors that influencing the customer loyalty. The service quality of the operator is supported by the simplicity and interactivity factors that examined by the author. The service quality factors of the operators will affect the customers in terms of brand trust, which in turn will affect the customer brand loyalty. The aim of the research are to measure the effect of simplicity and interactivity on the quality of service and its impact toward brand trust and brand loyalty. This research was conducted by using the marketing management approach. The primary data are used obtained from the respondents by using questionnaires and the number of customers that is a secondary data were collected from the annual report of the operators giving a picture of the customer loyalty indicator. The descriptive and verificative methods are used in this research. These measurements were performed by using the analytical techniques of SEM (Structural Equation Modeling).
The results showed that the Simplicity and Interactivity has a positive impact on the service quality of the operator. Quality of service also has a positive impact on brand trust and brand loyalty. The trust on cost that is an elements of brand trust need a special attention from the operator.The switching cost that is a supporting elements of brand loyalty is also need a special attention from the cellular operators.
Keywords: Simplicity, Interactivity, Service Quality, Brand Trust and Brand Loyalty
ABSTRAK
Tjahjono Djatmiko (1308209) “Analisis Simplisitas Dan Interaktivitas Dalam Membangun Kepercayaan Merek (Studi pada Pelanggan Telepon Seluler di DKI Jakarta dan Jawa Barat)” Disertasi Doktor Ilmu Manajemen Sekolah Pascasarjana Universitas Pendidikan Indonesia, dibawah bimbingan Prof. Dr. Hj. Ratih Hurriyati, MP dan Dr. Lili Adi Wibowo, S.Sos., S.Pd., MM.
Industri jasa telekomunikasi seluler di Indonesia berkembang sangat pesat. Terdapat tiga operator telekomunikasi seluler besar yang mendominasi pelayanan jasa ini. Perkembangan jumlah pelanggan yang dilayani oleh masing masing operator telekomunikasi seluler ini setiap tahun selalu berfluktuasi. Loyalitas pelanggan dari masing masing operator besar tersebut tidak sama. Loyalitas yang selalu tidak tetap dari pelanggan operator seluler tersebut menggambarkan adanya kualitas layanan dari operator telekomunikasi seluler yang belum memenuhi kebutuhan pelanggan yang pada akhirnya menjadi penyebab ketidak loyalan pelanggan tersebut. Kualitas layanan operator tersebut ditunjang oleh factor simplisitas dan interaktivitas yang kemudian diteliti oleh penulis. Faktor kualitas layanan operator tersebut akan mempengaruhi pelanggan dalam hal kepercayaan merek yang pada akhirnya akan mempengaruhi loyalitas merek pelanggan.
Penelitian ini bertujuan untuk mengukur pengaruh simplisitas dan interaktivitas terhadap kualitas layanan yang selanjutnya akan berpengaruh terhadap kepercayaan merek dan loyalitas merek
Penelitian ini dilakukan dengan menggunakan pendekatan ilmu manajemen pemasaran. Data yang dipergunakan adalah data primer berupa kuesioner yang diperoleh dari responden dan data sekunder jumlah pelanggan yang memberikan gambaran kondisi loyalitas pelanggan terhadap operator telekomunikasi tersebut. Metoda penelitian yang dipergunakan deskriptif dan verifikatif. Pengukuran ini dilakukan dengan mempergunakan teknik analisis SEM (Structural Equation Modelling). Hasil penelitian menunjukkan bahwa Simplisitas dan Interaktivitas berpengaruh positif terhadap kualitas layanan operator. Kualitas layanan juga mempunyai pengaruh positif terhadap kepercayaan merek dan loyalitas merek. Kepercayaan terhadap biaya yang merupakan unsur pendukung kepercayaan merek perlu mendapat perhaian dari operator. Biaya peralihan yang merupakan unsur pendukung loyalitas merek juga perlu mendapat perhatian dari operator telekomunikasi seluler.
Kata kunci: Simplisitas, Interaktivitas, Kualitas Layanan, Kepercayaan Merek dan Loyalitas Merek.
KATA PENGANTAR
Atas berkat rakhmat Allah SWT penulis dapat menyelesaikan tugas menyusun disertasi ini. Penulis mengucapkan puji syukur kehadirat Allah SWT karena hanya dengan rakhmat dan hidayahnya sajalah penulis dapat menyelesaikannya.. Disertasi ini merupakan salah satu persyaratan yang harus dipenuhi untuk memperoleh gelar Doktor Ilmu Manajemen Sekolah Pascasarjana Universitas Pendidikan Indonesia
Disertasi yang diselesaikan penulis berjudul Analisis Simplisitas Dan Interaktivitas Dalam Membangun Kepercayaan Merek (Studi pada Pelanggan Telepon Seluler di DKI Jakarta dan Jawa Barat). Disertasi ini berisi tentang permasalahan yang dihadapi oleh operator jasa telekomunikasi seluler di Indonesia terkait dengan loyalitas merek dari pelanggan telekomunikasi seluler. Disertasi ini membahas tentang simplisitas, interaktivitas, kualitas layanan, kepercayaan merek serta loyalitas merek dari pelanggan telekomunikasi seluler. Disertasi ini disusun menjadi lima bab yaitu (1) Pendahuluan (2) Kajian Pustaka (3) Metode Penelitian (4) Hasil Penelitian dan Pembahasan serta (5) Kesimpulan dan Rekomendasi.
Penulis berharap dengan adanya disertasi ini dapat memberi manfaat bagi khasanah Ilmu Pengetahuan dan dunia bisnis pada umumnya serta pada cabang ilmu manajemen pemasaran pada khususnya
Bandung Juni 2017
Tjahjono Djatmiko
UCAPAN TERIMAKASIH
Puji Syukur penulis panjatkan kehadirat Allah SWT karena berkat rakhmat dan hidayahNya penulis dapat menyelesaikan penyusunan disertasi ini. Disertasi ini berjudul “ANALISIS SIMPLISITAS DAN INTERAKTIVITAS DALAM MEMBANGUN KEPERCAYAAN MEREK (Studi pada Pelanggan Telepon Seluler di DKI Jakarta dan Jawa Barat).
Pada kesempatan ini penulis mengucapkan terimakasih yang sebesar besarnya dan penghargaan yang setingi tingginya kepada :
1. Bapak Prof. Dr H. Asep Kadarohman M.Si, selaku Rektor Universitas Pendidikan Indonesia
2. Bapak Prof. Dr. H. Yaya Sukjaya Kusumah, M. Sc, selaku Direktur Sekolah Pascasarjana Universitas Pendidikan Indonesia
3. Bapak Prof. Dr. H. Agus Rahayu, M.P. selaku Dekan Fakultas Pendidikan Ekonomi dan Bisnis Universitas Pendidikan Indonesia.
4. Bapak Prof. Dr. H Disman M.S, Selaku Pimpinan Sidang Terbuka Ujian Promosi Doktor mewakili Direktur Sekolah Pascasarjana Universitas Pendidikan Indonesia
5. Bapak Prof. Dr. H. Eeng Ahman, MS selaku Ketua Program Studi Magister Manajemen Bisnis dan Doktor Ilmu Manajemen Universitas Pendidikan Indonesia.
6. Ibu Prof. Dr Hj. Ratih Hurriyati, M.P, selaku Ketua Promotor yang telah banyak membimbing dan memberikan pengarahan kepada penulis dalam menyelesaikan penulisan disertasi ini.
7. Bapak Dr. Lili Adi Wibowo, S.Sos., S.Pd., M.M, selaku Ko Promotor yang telah banyak membimbing dan memberikan pengarahan kepada penulis dalam menyelesaikan penulisan disertasi ini.
8. Ibu Prof Dr. Hj. Tjutju Yuniarsih SE, M.Pd selaku ketua Komisi Pascasarjana yang telah banyak memberikan masukan untuk perbaikan disertasi ini
9. Bapak Prof. Dr. H. Eeng Ahman, MS, Selaku penguji yang telah memberikan banyak masukan bagi kesempurnaan disertasi ini
10. Ibu Dr. Vanessa Gaffar MBA Selaku penguji yang telah memberikan banyak masukan bagi kesempurnaan disertasi ini
11. Bapak Dr. Husni Amani, M.M., M.Sc. Selaku penguji yang telah memberikan banyak masukan bagi kesempurnaan disertasi ini
12. Bapak dan Ibu Dosen program studi Magister Manajemen dan Doktor Ilmu Manajemen yang telah memberikan perkuliahan kepada penulis pada perioda perkuliahan 2013-2015
13. Bapak Ir Dodie Tricahyono MM, PhD. selaku Dekan Fakultas Ekonomi dan Bisnis Universitas Telkom dan jajarannya yang telah memberikan kesempatan kepada penulis untuk melanjutkan studi S3 ini.
14. Bapak dan ibu dosen Universitas Telkom yang telah banyak memberikan support serta masukan dalam penyelesaian tugas akademis bagi penulis.
15. Bapak dan ibu rekan-rekan satu angkatan DIM UPI 2013/2014 yang sangat kompak dalam menyelesaikan tugas tugas akademis dari Universitas Pendidikan Indonesia
16. Keluarga besar penulis, terutama Isteri dan anakku yang telah banyak memberikan support untuk penyelesaian tugas ini.
17. Responden yang telah dengan rela memberikan masukannya dalam pengumpulan data baik di DKI Jakarta maupun di Jawa Barat
18. Bapak/ibu yang tidak dapat penulis sebutkan satu persatu dalam kesempatan ini yang telah banyak memberikan bantuannya baik materiel maupun moril
Kepada beliau yang disebutkan diatas semoga Allah SWT berkenan memberi balasan atas kebaikan kebaikan dan support yang telah disampaikan kepada penulis. Aamiin Ya Rabbal Aalamiin
Bandung, Juni 2017
Tjahjono Djatmik
Efecto de los periodontopatógenos sobre el perfil inflamatorio de células endoteliales de la arteria aorta
No full textIntroducción: Se han encontrado respuestas variables en la expresión de citoquinas proinflamatorias como IL-1B e IL-6 en diferentes tejidos expuestos a periodontopatógenos como P. gingivalis. La mayoría de los estudios actuales han utilizado cepas de P. gingivalis, mientras que unos pocos evaluaron periodontopatógenos diferentes. Este estudio tuvo como objetivo evaluar el efecto de algunos periodontopatógenos sobre el perfil inflamatorio de células endoteliales de la arteria aorta. Objetivo: Evaluar el efecto de algunos periodontopatógenos sobre el perfil inflamatorio de células endoteliales de la arteria aorta. Metodología: Estudio experimental in vitro unifactorial multivariado. Las células endoteliales de la arteria aorta se sembraron en placas de fondo redondo de 96 pocillos a 5x105 células/pocillo en 200 ul de RPMI (Sigma-Aldrich) suplementada con suero bovino fetal (FBS) al 10%. Posteriormente, las células fueron estimuladas con tres bacterias orales, Streptoccocus sanguinis spp y Aggregatibacter actinomicetencomitans spp y LPS de Porphyromonas gingivalis. Las bacterias se añadirán al cultivo celular a una MOI de 100. Se incluyó LPS a 50 ng/ml como control positivo. Las células se incubaron a 37 °C, CO2 al 5 % durante 18 horas. Posteriormente se realizó la cuantificación del ARNm de las interleucinas IL1B, IL6, IL8 y de la quimiocina RANTES/CCL5 por RT-PCR en tiempo real. También se hizo la medición de la expresión a nivel de proteínas de IL1-B e IL-6 mediante pruebas ELISA. Resultados: En cuanto a la expresión de ARNm, después de la exposición con A. actinomycetemcomitans el nivel de expresión de IL1B fue 47,9 veces significativamente mayor comparado con el control (p = 0.023) y el nivel de expresión de IL8 fue significativamente mayor comparado con el control 4,6 veces (p = 0.0003). Después de la exposición a S. sanguinis la expresión de IL6 fue significativamente menor, 3,33 veces (p = 0.0007) y la expresión de RANTES fue significativamente mayor 30,2 veces (p = 0.0004). Cuando las células fueron expuestas a LPS de P. gingivalis la expresión de IL1B aumentó significativamente 17,9 veces (p = 0.032), la expresión de IL8 fue significativamente mayor 10,5 veces (p = 0.002) y la expresión de RANTES fue significativamente mayor 27,2 veces (p = 0.002). En cuanto al nivel de la expresión de proteínas en el control la expresión de Il-1B fue de 26,7 pg/ml y después de la exposición a A. actinomicetencomitans spp, S. sanguinis spp y LPS de P. gingivalis el nivel aumento 5.9, 6.3 y 8.9 veces, respectivamente. A nivel de proteína la expresión de IL-6 en el control fue de 48,3 pg/ml ml y después de la exposición a A. actinomicetencomitans spp, S. sanguinis spp y LPS de P. gingivalis el nivel aumento 5.4, 5.7 y 8.1veces, respectivamente. Conclusión: Nuestro estudio demostró que los periodontopatógenos S. sanguinis, A. actinomycetecomitans y el LPS de P. gingivalis pueden estimular a las células endoteliales de las arterias coronarias para incrementar los niveles del ARNm de las citoquinas proinflamatorias estudiadas (IL-1B, IL-6, IL-8 y RANTES). Además, se observó un aumento en la expresión de las proteínas proinflamatorias IL-1B e IL-6 con la interacción con todos los periodontopatógenos estudiados respecto al grupo control, lo que sugiere que estas bacterias orales pueden favorecer la inflamación de los tejidos endoteliales y podrían relacionarse con la formación y agravamiento de las placas de ateroma.0000-0003-0336-96820009-0007-0205-99530009-0003-6994-2012sandra.guauque@campusucc.edu.covanessa.oquendop@[email protected]
