2,145 research outputs found
Clara Fry letter, September 28, 1914
No full textClara Fry wrote this letter on September 28, 1914, to request literature in support of women's suffrage to be sent to Burton, Ohio.
The Franklin County Woman Suffrage Association was formed in 1912, after the Ohio Constitutional Convention elected to bring to a vote the question of removing the words "white male" from the state constitution with regard to voting rights. Headquartered in the Chamber of Commerce building in Columbus, Ohio, the organization put out regular publications, organized public speeches and meetings, distributed literature and held parades in support of the suffrage movement. Women's suffrage in Ohio was defeated in a special election in 1912 and again in 1914 and 1916 before a resolution narrowly passed in 1917 allowing municipal voting by women in Columbus. In 1920, the 19th Amendment passed, extending the vote to women and prohibiting state and federal government from denying suffrage on the basis of sex
Spirit of light, so fondly courted, once heav'nly bright [first line]
No full textstrophicpiano and voice770Johns Hopkins University, Levy Sheet Music Collection, Box
128, Item 076Translated from the Italian by J.R. Fry. Music by G. Donizetti.R.M. Gaw, Engr
Spirit of light, so fondly courted, once heav'nly bright [first line]
No full textstrophicpiano and voice770Johns Hopkins University, Levy Sheet Music Collection, Box
128, Item 076Translated from the Italian by J.R. Fry. Music by G. Donizetti.R.M. Gaw, Engr
Hill Creek Spawning Channel Kokanee Fry Enumeration, Spring 1987
No full textWildland RecreationIn the spring of 1987, Kokanee fry enumeration was conducted at the Hill Creek spawning channel. An estimated 4, 000, 000 fry were produced resulting in an egg-fry survival rate of 68.7
Fishing gear for prawn and shrimp used in the Philippines today
No full textThe report describes the different gears used for collecting penaeids which have been traditionally used in the Philippines. Twenty gears are presently found throughout the Philippines: nine for collecting sugpo fry and 13 for capturing adult prawns. Thirty-six figures are included in this report. The illustrations were done by Mr. Panfilo Legazpi, Jr., a scientific illustrator at SIA, based on colored photographs and actual observations.
Also in: Compilation of JICA Expert Activities at SEAFDEC, Aquaculture Department (1974-1985) by S. Fukumoto, Japan International Cooperation Agency (JICA) 1989, pp 63-75.This paper describes various types of shrimping and prawning gear and devices, most of which have been traditionally used in the Philippines, with some ecological notes. This study provides basic information on prawn culture and fry collection, which will be useful for private fishpond operators and workers
Diel migration pattern of pink salmon fry in small streams
No full textDownstream migration is a critical stage in the anadromous salmonid life cycle, but previous studies have shown different results between rivers or surveys for the diel downstream migration pattern of the fry of the pink salmon Oncorhynchus gorbuscha. We investigated the diel migration pattern of pink salmon fry in three small streams. Our results showed that pink salmon fry migrate mainly within a few hours after sunset; 89.9% of migration occurred between 18:00 and 23:00. Therefore, the results indicated that sunset time influences the diel migration pattern of pink salmon fry in small streams. This pattern could be a predator-avoidance behaviour
Epigenetic regulation of Fry: a mammary carcinoma susceptibility gene
No full textFry is an evolutionarily-conserved gene involved in cellular differentiation, division, morphology, polarity, and adhesion. Decreased expression of the FRY protein in breast cancer cell lines reduced the expression of gene networks associated with epithelial differentiation, morphology, polarity and adhesion. Ectopic expression of the wild-type rat Fry gene in human breast cancer cells restored a gene expression profile associated with differentiation, suppression of epithelial-mesenchymal transition. Breast cancer cells expressing wild-type Fry at physiological levels reacquired a non-transformed morphology in vitro and a non-tumorigenic in vivo phenotype in the nude mouse xenograft model. We further determined that expression of FRY mRNA and protein correlated with breast cancer phenotypes such as Elston tumor grade, estrogen receptor, progesterone receptor, and Her2 receptor status. Despite these finding, significant gaps in knowledge remain in our understanding of how FRY expression is decreased during tumor progression. Based on preliminary data, we hypothesized modulation of FRY expression occurs through epigenetic mechanisms which include DNA methylation, histone modification and chromatin remodeling, and the activity/interaction of non-coding RNAs. To understand how DNA methylation regulates FRY transcription in breast tumors, we examined publicly available RNA-seq and DNA methylation data from The Cancer Genome Atlas (TCGA). Analysis of FRY promoter methylation revealed no significant changes in DNA methylation in normal vs. breast tumors. However, when we subdivided the tumors by hormone receptor status, we observed an increase in FRY promoter methylation in ER- and PR- breast tumors. Treatment of various breast cancer cell lines with the DNMT inhibitor, 5-Aza-2-deoxycytidine, resulted in upregulation of FRY mRNA and protein expression. These results indicate that loss of FRY expression may in part be due to increased methylation. To elucidate the role of chromatin in the role of FRY expression we searched publicly available chromatin immunoprecipitation sequencing data from the Encyclopedia of DNA elements (ENCODE) to identify key chromatin markers and transcription factors that bind to the FRY promoter. Using this data, we demonstrated that FRY has a canonical gene promoter characterized by low levels of H3K4me1 and high levels H3K4me2/me3. Furthermore, using data from the ENCODE, we identified 28 transcription factors predicted to binds to the FRY gene promoter, the most significant of which were estrogen receptor 1 (ESR1) and progesterone receptor (PR). To determine if histone deacteylases (HDACs) impacted FRY expression, we dosed various breast cancer cell lines with commonly used the HDAC inhibitors, Trichostatin-A and Panobinostat (LBH589). Through treatment of our cell lines with these HDAC inhibitors, we were able to modulate FRY expression, indicating that its expression is in part regulated by protein acetylation. Next, we explored whether transcriptional repressor, enhancer of Zeste Homolog 2 (EZH2) can bind to the FRY promoter to down regulate its expression. Using Chromosome Immunoprecipitation DNA sequencing (ChIP-seq) data from the ENCODE database, we identified that EZH2 binds to the FRY promoter in both normal human mammary epithelial cells (HMECs) and in the breast cancer derived MCF-7 cell line. Furthermore, when we treated cells with EZH2 inhibitors, GSK343 and DZNep, we induce the expression of FRY mRNA and protein in the MDA-MB231, MDA-MB-468, MCF-7, T47-D and HCC1954 cell lines. Our results suggest that elevated expression of EZH2 contributes to decreased FRY expression in breast cancer cells. One unique feature of the FRY gene promoter is that it also encodes an antisense long coding RNA (lncRNA), FRY-AS1. Many lncRNAs have been shown to be biologically active and have been implicated various cellular processes such as transcriptional interference, the induction of chromatin remodeling, and modulation of protein activity. To date, no studies have focused on the functional characterization of FRY-AS1. In this present study, we analyzed publicly available RNA-Seq data. We found that FRY-AS1 is differentially expressed in a variety of human tissue, and that FRY mRNA expression is strongly correlated with FRY-AS1 expression. Next, using RNA-seq data from TCGA BRCA dataset, we found that FRY-AS1 expression was significantly decreased in basal-like tumors, and in estrogen negative, and progesterone negative tumors. In vitro studies indicated that FRY-AS1 was decreased in 4 of 5 cell lines examined relative to the levels non-tumorigenic mammary epithelial cell line. Cellular fractionation studies revealed that FRY-AS1 is primarily localized to the nucleus. Ectopic over-expression of FRY-AS1 in the MDA-MB-231 induced differentiation to a more differentiated epithelial phenotype in vitro and induced the formation the formation acinar structures in 3D-Matrigel cultures. Significantly, overexpression of FRY-AS1 also increased endogenous FRY expression, indicating that the lncRNA regulates FRY expression in trans. Lastly, we treated the MDA-MB-231, MDA-MB-468, MCF-7, HCC1954, and T47-D cell lines with HDAC, DNMT, or EZH2 inhibitors. In these studies, we were able to induce FRY-AS1 expression in all cancer cell lines examined, which indicates that the expression of this lncRNA is regulated in part through multiple epigenetic mechanisms. Taken together, our results demonstrate that FRY-AS1 is a bona-fide lncRNA with functional significance in regulation of the FRY carcinoma susceptibility gene, and hence may be a target therapeutic intervention.Ph.D.Includes bibliographical referencesby Brian Estrell
Garrett and Fry Lake Cave Inventory
No full textThe following report is compiled to provide an inventory of the caves in the Garrett and Fry Lakes region. For the purposes of the inventory all caves known or found in the inventory areas are physically located. Later all entrances are tied together in an interentrance survey and the cave entrances marked on a location map
An uncommon presentation of fungal infection in Atlantic salmon fry
No full textFungal infection in fry from several families of Atlantic salmon Salmo salar are reported. Moribund fry had a conspicuous cranial defect that corresponded with 18.3% mortality. The lesion was characterized by a domed, epithelium-covered swelling centered over the optic tectum. The swelling was an oedematous reaction to the extension of a fulminating necrotizing mycotic stomatitis and branchitis into the cranial tissues including the ventral meninges. The genus and species of the fungal pathogen were not identified, although histological examination was consistent with an oomycete. The % mortality of fry in each separately reared family ranged from 1.0 to 63.3%. An analysis of variance revealed significant differences in mortality among families (P http://upei-resolver.asin-risa.ca?sid=SP:CABI&id=pmid:&id=&issn=0899-7659&isbn=&volume=3&issue=3&spage=192&pages=192-197&date=1991&title=Journal%20of%20Aquatic%20Animal%20Health&atitle=An%20uncommon%20presentation%20of%20fungal%20infection%20in%20Atlantic%20salmon%20fry.&aulast=Hanke&pid=%3Cauthor%3EHanke%2c%20A%20R%3bBackman%2c%20S%3bSpeare%2c%20D%20J%3bFriars%2c%20G%20W%3C%2Fauthor%3E%3CAN%3E19941200171%3C%2FAN%3E%3CDT%3EJournal%20article%3C%2FDT%3
GENERATION Y AND VOCAL FRY
No full textIn this thesis, the author explores a vocal phenomenon called “vocal fry” and why this dysphonia has emerged as one of the primary means of communication for the population referred to as “Generation Y.” The first chapter defines what vocal fry is and why it is of modest value to the stage actor. The second chapter defines the physiological means by which the human body creates sound, what good sound is and how vocal fry is created. The third and fourth chapters define several generations as well as what defines Gen Y, and the communication physiologies associated with those generations. The last chapter discusses the rise of narcissism within Gen Y and how this may relate to the arrival of vocal fry as a default register for theatre students. The conclusion outlines several solutions that may alleviate the problems associated with this dysphonia
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