10 research outputs found
Evaluación del poder antiséptico del cemento de Grossman combinado con yodoformo sobre distintas cepas bacterianas encontradas en infecciones de origen endodóntico
No full textEl objetivo de este estudio fue evaluar in vitro el poder antiséptico del cemento de Grossman, un material de obturación muy utilizado en endodoncia, en combinación con 2 proporciones de yodoformo, sobre bacterias comúnmente encontradas en infecciones de origen endodóntico. Resultados: el yodoformo puro no evidenció capacidad antiséptica sobre ninguna de las cepas estudiadas. Sin embargo, al asociarse con CG incrementó su efecto inhibitorio sobre Streptococcus mutans y sobre Enterococcus faecalis, en forma concentración-dependiante.Fil: Buldo, Mauro. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia. Buenos Aires, Argentina.Fil: Elverdin, J.C. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología. Buenos Aires, Argentina.Fil: Fernández-Solari, J. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología. Buenos Aires, Argentina.Fil: Rodríguez, P. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia. Buenos Aires, Argentina
Utilización de membrana de fascia lata y hueso en polvo liofilizado en cirugía apical: caso clínico
No full textEn un paciente con patología apical y endodoncia previa en la pieza 1.2, se realizó cirugía apical que incluyó el agregado de una membrana biológica reabsorbible de fascia lata entre el colgajo de espesor total y el tejido óseo. En medio de ellos, se colocó hueso en polvo humano liofilizado con el objetivo de proveer una adecuada recuperación del tejido óseo perirradicular. Luego, se efectuó el control clínico y radiográfico del paciente durante 24 meses.Fil: Rodríguez, P.A. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia. Buenos Aires, Argentina.Fil: Lenarduzzi, A.L. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia. Buenos Aires, Argentina.Fil: Fernández-Solari, J. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología. Buenos Aires, Argentina.Fil: Elverdin, J.C. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología. Buenos Aires, Argentina
Hiposialia por activación de receptores de cannabinoides en la glándula submaxilar. Revisión
No full textEl presente trabajo aporta evidencia de la presencia de receptores de cannabinoides en la glándula submaxilar de la rata, cuya expresión se circunscribe a componentes acinares y ductales. A su vez, los resultados expuestos confirman la participación de los receptores de cannabinoides en el control de la secreción salival, y por ende aportan una explicación empírica a la hiposialia observada luego del consumo de marihuana.Fil: Fernández Solari, J. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología. Buenos Aires, Argentina / CONICET, Argentina.Fil: Ossola, C.A. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología. Buenos Aires, Argentina.Fil: Prestifilippo, J.P. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología. Buenos Aires, Argentina.Fil: Elverdin, J.C. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología. Buenos Aires, Argentina
Tratamiento endodóntico obturado con pasta lentamente reabsorbible: seguimiento de un caso a 6 años
No full textReporte de caso clínico de un tratamiento endodóntico de incisivo central superior con diagnóstico de necrosis pulpar y proceso apical preexistente, obturado con pasta lentamente reabsorbible. El seguimiento post tratamiento fue realizado mediante controles radiográficos inmediatos y a distancia, en los cuales se evaluó la calidad de la obturación y la reparación de los tejidos dañados.Fil: Buldo, M.A. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia. Buenos Aires, Argentina.Fil: Corominola, P.L. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia. Buenos Aires, Argentina.Fil: Martínez, P.A. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia. Buenos Aires, Argentina.Fil: Lenarduzzi, A.L. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia. Buenos Aires, Argentina.Fil: Fernández Solari, J. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología. Buenos Aires, Argentina.Fil: Elverdin, J.C. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología. Buenos Aires, Argentina.Fil: Rodríguez, P.A. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Endodoncia. Buenos Aires, Argentina
Endocannabinoids mediate hyposalivation induced by inflammogens in the submandibular glands and hypothalamus
No full textObjective: The aim of this study was to investigate the factors that could participate on salivary glands hypofunction during inflammation and the participation of endocannabinoids in hyposalivation induced by the presence of inflammogens in the submandibular gland (SMG) or in the brain. Design: Salivary secretion was assessed in the presence of inflammogens and/or the cannabinoid receptor antagonist AM251 in the SMG or in the brain of rats. At the end of the experiments, some systemic and glandular inflammatory markers were measured and histopathological analysis was performed. Results: The inhibitory effect observed 1 h after lipopolysaccharide (LPS, 50 μg/50 μl) injection into the SMG (ig) was completely prevented by the injection of AM251 (5 μg/50 μl) by the same route (P < 0.05). The LPS (ig)-induced increase in PGE2 content was not altered by AM251 (ig), while the glandular production of TNFa induced by the endotoxin (P < 0.001) was partially blocked by it. Also, LPS injection produced no significant changes in the wet weight of the SMG neither damage to lipid membranes of its cells, nor significant microscopic changes in them, after hispopathological analysis, compared to controls. Finally, TNFα (100 ng/5 μl) injected intracerebro-ventricularly (icv) inhibited methacholine-induced salivary secretion evaluated 30 min after (P < 0.01), but the previous injection of AM251 (500 ng/5 μl, icv) prevented completely that effect. Conclusion: We conclude that endocannabinoids mediate the hyposialia induced by inflammogens in the SMG and in the brain. The hypofunction would be due to changes on signalling pathway produced by inflammatory compounds since anatomical changes were not observed. © 2013 Elsevier Ltd. All rights reserved.Fil: Prestifilippo, Juan Pablo. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; ArgentinaFil: Medina, Vanina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; ArgentinaFil: Mohn, Claudia Ester. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Rodriguez, P. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Unidad de Administración Territorial; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Elverdin, Juan Carlos. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología; ArgentinaFil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Fisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentin
Pharmacological targeting of histamine H4 receptor in periodontal disease
No full textObjective: The objective of this study was to investigate whether histamine H4 receptor (H4R) antagonists could prevent experimental periodontitis (EP)-induced histological, functional and inflammatory alterations in submandibular gland (SMG), periodontal bone and gingiva. Methods: Bilateral EP was induced for 2 weeks in anaesthetized male rats. The effect of systemic and local administration of H4R antagonists (JNJ7777120, JNJ10191584) on histopathology and functionality of SMG, bone loss and gingival inflammation was evaluated. Results: The subcutaneous administration of JNJ7777120 prevented periodontitis-induced SMG histological injury, reducing vacuolization and apoptosis and additionally reversed the increased prostaglandin E2 (PGE2) levels in SMG while it partially reversed the methacholine-induced salivation reduction produced by periodontitis. JNJ7777120 attenuated bone loss and the increased PGE2 levels and inflammatory infiltration in gingival tissue of rats with periodontitis. Finally, local administration of JNJ7777120 and JNJ10191584 was also beneficial for improving periodontal parameters. Conclusions: H4 receptor antagonists are able to ameliorate periodontitis-induced injury on SMG, gingival tissue and bone structure, suggesting that pharmacological targeting of H4R could be an attractive strategy to improve periodontal health.Fil: Prestifilippo, J. P.. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Fernández Solari, José Javier. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinel Lamas, Diego José. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Rios, Carlos Ezequiel. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mohn, Claudia Ester. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perazzo, Juan C.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Rivera, E. S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Elverdin, J. C.. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentin
Participation of hypothalamic CB1 receptors in reproductive axis disruption during immune challenge
No full textImmune challenge inhibits reproductive function and endocannabinoids (eCB) modulate sexual hormones. However, no studies have been performed to assess whether the eCB system mediates the inhibition of hormones that control reproduction as a result of immune system activation during systemic infections. For that reason, we evaluated the participation of the hypothalamic cannabinoid receptor CB1 on the hypothalamic-pituitary-gonadal (HPG) axis activity in rats submitted to immune challenge. Male adult rats were treated i.c.v. administration with a CB1 antagonist/inverse agonist (AM251) (500 ng/5 μL), followed by an i.p. injection of lipopolysaccharide (LPS) (5 mg/kg) 15 minutes later. Plasmatic, hypothalamic and adenohypophyseal pro-inflammatory cytokines, hormones and neuropeptides were assessed 90 or 180 minutes post-LPS. The plasma concentration of tumour necrosis factor α and adenohypophyseal mRNA expression of Tnfα and Il1β increased 90 and 180 minutes post i.p. administration of LPS. However, cytokine mRNA expression in the hypothalamus increased only 180 minutes post-LPS, suggesting an inflammatory delay in this organ. CB1 receptor blockade with AM251 increased LPS inflammatory effects, particularly in the hypothalamus. LPS also inhibited the HPG axis by decreasing gonadotrophin-releasing hormone hypothalamic content and plasma levels of luteinising hormone and testosterone. These disruptor effects were accompanied by decreased hypothalamic Kiss1 mRNA expression and prostaglandin E2 content, as well as by increased gonadotrophin-inhibitory hormone (Rfrp3) mRNA expression. All these disruptive effects were prevented by the presence of AM251. In summary, our results suggest that, in male rats, eCB mediate immune challenge-inhibitory effects on reproductive axis at least partially via hypothalamic CB1 activation. In addition, this receptor also participates in homeostasis recovery by modulating the inflammatory process taking place after LPS administration.Fil: Surkin, Pablo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Di Rosso, María Emilia. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Correa, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Elverdín, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Genaro, Ana Maria. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: de Laurentiis, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Fernández Solari, José Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentin
Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission
No full textOur objective was to determine the effect of arachidonylethanolamide (anandamide, AEA) injected intracerebroventricularly (icv) into the lateral ventricle of the rat brain on submandibular gland (SMG) salivary secretion. Parasympathetic decentralization (PSD) produced by cutting the chorda tympani nerve strongly inhibited methacholine (MC)-induced salivary secretion while sympathetic denervation (SD) produced by removing the superior cervical ganglia reduced it slightly. Also, AEA (50 ng/5 µL, icv) significantly decreased MC-induced salivary secretion in intact rats (MC 1 µg/kg: control (C), 5.3 ± 0.6 vs AEA, 2.7 ± 0.6 mg; MC 3 µg/kg: C, 17.6 ± 1.0 vs AEA, 8.7 ± 0.9 mg; MC 10 µg/kg: C, 37.4 ± 1.2 vs AEA, 22.9 ± 2.6 mg). However, AEA did not alter the significantly reduced salivary secretion in rats with PSD, but decreased the slightly reduced salivary secretion in rats with SD (MC 1 µg/kg: C, 3.8 ± 0.8 vs AEA, 1.4 ± 0.6 mg; MC 3 µg/kg: C, 14.7 ± 2.4 vs AEA, 6.9 ± 1.2 mg; P < 0.05; MC 10 µg/kg: C, 39.5 ± 1.0 vs AEA, 22.3 ± 0.5 mg; P < 0.001). We showed that the inhibitory effect of AEA is mediated by cannabinoid type 1 CB1 receptors and involves GABAergic neurotransmission, since it was blocked by previous injection of the CB1 receptor antagonist AM251 (500 ng/5 µL, icv) or of the GABA A receptor antagonist, bicuculline (25 ng/5 µL, icv). Our results suggest that parasympathetic neurotransmission from the central nervous system to the SMG can be inhibited by endocannabinoid and GABAergic systems
Investigation of a role for noradrenaline in the neurochemical and behavioural effects of an established and putative target for antianxiety/antidepressant drugs: a microdialysis and behavioural study in rats and NK1 receptor knockout mice.
No full textThe precise neurochemical effects of antidepressants that underlie their efficacy remain unclear. Many antidepressants modulate noradrenaline transmission in vivo. However, the selective serotonin reuptake inhibitors (SSRIs) and NK1 receptor antagonists target 5-HT and substance P transmission, respectively. Whether effects on noradrenaline neurones contribute to the efficacy of these drugs has been largely neglected. This study investigates modulation of central NA transmission by an SSRI (fluoxetine) in rats and by NK1 receptor ablation in mice. NK1 'knockout' mice are used as a model for lifelong inhibition of the NK1 receptor. Changes in noradrenaline transmission were determined using in vivo microdialysis to sample the extracellular noradrenaline concentration (efflux). Noradrenaline-related behavioural effects of these treatments were determined using a modified light/dark exploration test. Systemic fluoxetine increased noradrenaline efflux in two brain regions (frontal cortex and hypothalamus). The effect was inconsistent, echoing the 5-HT response to SSRIs. Fluoxetine reduced noradrenaline efflux after partial lesioning of noradrenergic axon terminals (by DSP-4), suggesting an inhibitory action upstream of these terminals. Locally infused (retrodialysis) fluoxetine augmented noradrenaline efflux in the terminal field. Whereas 50uM fluoxetine elevated efflux in both regions, 5uM infusion increased efflux in the frontal cortex only. This effect was 5-HT-dependent (i.e. 5-HT synthesis inhibition with pCPA blocked the response). However, this increase was not secondary to inhibition of 5-HT reuptake (since the more potent and selective SSRI, citalopram, did not change noradrenaline efflux). Thus, fluoxetine has opposing effects on noradrenaline efflux, probably at cell bodies and terminals. The balance of processes could underlie its variable effects. Fluoxetine reduced locomotor activity of DSP-4-pretreated rats in the novel compartment of the exploration box, suggesting a noradrenaline-related effect on behaviour. A 5-HT-related effect of fluoxetine was also found (reduced locomotor activity in the dark compartment), since this was abolished by pCPA
Co-designing crop diversification strategies from plot to sociotechnical system to manage root knot nematodes in Mediterranean market gardening systems
No full textThis is my presentation at the Cropdiversification Conference Session1 "Co-designing crop diversification: which actors to include beyond the farm?".
ABSTRACT
Co-designing crop diversification strategies from plot to sociotechnical system to manage root-knot nematodes in Mediterranean market gardening systems
Yann Boulestreau∗±1,3, Marion Casagrande 1,2, Mireille Navarrete 1
1 INRA UR767, Ecodéveloppement, 228 route de l’aérodrome, 84914 Avignon cedex 09, France
2 ITAB, 525 route de Gotheron 26320 Saint Marcel les Valence, France
3 ADEME, 20 Avenue du Grésillé, 49000 Angers, France
∗Speaker
± Corresponding author: [email protected]
1 Introduction
Root-knot nematodes (RKN) are causing major yield losses in Mediterranean sheltered market gardening systems on the dominant crops (e.g. tomatoes, melon, lettuce). Climate change and crop specialization are the main causes of increased RKN damages. With the ban of most synthetic nematicides due to their toxicity, no unique and simple technique to control RKN exists. Crop diversification has been identified as one of the main alternatives. Crop diversification consists in introducing in crop successions non-host, trap or allelopathic crops for commercial and non-commercial purposes. However, scientific literature (Magrini et al., 2016; Meynard et al., 2018) has shown that sociotechnical lock-in hinders crop diversification, calling for simultaneous and organized actions of multiple actors to unlock crop diversification. This communication shows a methodology to codesign such collective strategies to favor crop diversification, from plot to sociotechnical system. The design of strategies is based on previous analysis of the sociotechnical lock-in and on previous exploration of innovative strategies already implemented in other contexts. This communication shows how we applied this methodology in “Rhône-Durance-Vaucluse” territory, South East France, on sheltered market gardening systems.
2 Materials and Methods
First, we analysed the sociotechnical system (STS) on “Rhône-Durance-Vaucluse” territory responsible for the present soil pest and disease management implemented by market gardeners. We identified the actors with a strong influence on RKN management based on literature and on snowball sampling procedure (Salembier et al., 2016). To understand the main impediments to crop diversification and the sociotechnical lock-in around crop specialization, we interviewed 33 actors including 16 organic and conventional farmers, representatives of agricultural advisory services, wholesalers, input-suppliers and the head of the wholesale marketplace. We also reviewed white and grey literature, and led participatory observation of key meetings involving the STS actors. Then, we characterized existing innovative strategies in other contexts that could unlock crop diversification in ours, based on stakeholders interviews, literature review and key meeting observation.
Finally, we organized a collective design process of innovative strategies to unlock crop diversification with key STS actors. KCP methodology (Le Masson et al., 2009) was used as a 3-phase methodological framework for this collective design process. The Knowledge-phase consisted of sharing knowledge with participants about the RKN management techniques, the impediments for crop diversification, the lock-in of the STS and some existing innovative strategies in other contexts that could help them think “out-of-the-box” (e.g. potato farmers without land renting new fields from cereal farmers every year). A dynamic presentation of management technique posters by technical experts and simulations of the sociotechnical system evolution based on a serious game were used to share this knowledge. The Concept-phase was an oriented exploration of strategy prototypes with the same “serious game” (Fig.1.). The Project-phase will occur later in our research. K-phase and C-phase were started during two workshops with two different groups of farmers: (i) a mix of six organic and conventional farmers selling their products to various whole salers, (ii) a group of six organic farmers selling to the same wholesaler and the wholesaler’s crop planning manager.
3 Results
Besides market gardeners, the key stakeholders of the STS were the vegetable wholesalers and retailers, consumers, input-suppliers (seedlings, seeds), breeders, extension services (advisers, applied research stations), farmer cooperatives and agri-food chain coordinators. The main impediments identified for crop diversification were, from plot to agri-food chain level: unsuitable soils (e.g. too many stones for vegetable root crops), investment in tunnel or greenhouse limiting the species diversity that could be produced and a lack of local outlet for minor crops. The market-gardening sector was locked around the requirement to produce large volumes of a single product at low cost, especially for conventional production. Then, to satisfy brokers, middle size market gardeners needed to specialize in very few products. To unlock the system, innovative strategies were explored: coordination between farmers (e.g. exchange of fields between a RKN host plant producer and a non-host crop producer to diversify rotations without diversifying outlets) and coordination between farmers and brokers (e.g. the brokers open a new outlet enabling farmers to diversify their crops).
Thanks to the exchange with the STS stakeholders during the workshops, the understanding of the impediments for crop diversification and the lock-in was refined. Strategy prototypes were designed, integrating actions from plot to sociotechnical system. For instance, workshop participants proposed to insert new non-host crops such as spring garlic and artichoke in nematode infected plots in coordination with the brokers’ needs and other existing spring garlic or artichoke producers. Actions at farm level only were also proposed such as moving the shelter structure from a RKN infected plot to another healthy plot.
4 Discussion and Conclusions
To conclude, we engaged market-gardeners and other key STS actors from the “Rhône-Durance-Vaucluse” territory in a common effort to understand and to analyze STS lock-in and explore strategies from farm to STS levels that could unlock crop diversification. Realistic RKN management strategy prototypes adapted to “Rhône-Durance-Vaucluse” sheltered market-gardening systems were designed. Even if we focus on crop diversification in this communication, it needs to be combined with other RKN management techniques (e.g. fresh organic matter supply) to effectively manage RKN populations. The next challenge is now to evaluate how effective these agroecological strategies are to control RKN populations, how they would impact the STS, and to detail how they could be implemented. Another stakeholder workshop is planned in autumn 2019.
References
Le Masson, P., Hatchuel, A., Weil, B. (Eds.), 2009. Design theory and collective creativity: A theoretical framework to evaluate KCP process.
Magrini, M.-B., Anton, M., Cholez, C., Corre-Hellou, G., Duc, G., Jeuffroy, M.-H. et al, 2016. Why are grain-legumes rarely present in cropping systems despite their environmental and nutritional benefits?: Analyzing lock-in in the French agrifood system. Ecological Economics 126, 152–162. https://doi.org/10.1016/j.ecolecon.2016.03.024.
Meynard, J.-M., Charrier, F., Le Bail, M., Magrini, M.-B., Charlier, A., Messéan, A., 2018. Socio-technical lock-in hinders crop diversification in France. Agron. Sustain. Dev. 38, 54.
Meynard, J.-M., Messéan, A., Charlier, A., Charrier, F., Fares, M.'h., Le Bail, M. et al, 2013. Freins et leviers à la diversification des cultures: Étude au niveau des exploitations agricoles et des filières. OCL 20, D403. https://doi.org/10.1051/ocl/2013007.
Salembier, C., Elverdin, J.H., Meynard, J.-M., 2016. Tracking on-farm innovations to unearth alternatives to the dominant soybean-based system in the Argentinean Pampa. Agron. Sustain. Dev. 36, 1. https://doi.org/10.1007/s13593-015-0343-9.</p
