23 research outputs found

    Two new mathildids from the Mediterranean Sea (Gastropoda, Heterobranchia, Mathildidae)

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    Based on shell characters, two new gastropod species of the family Mathildidae Dall, 1889, are herein described from the Mediterranean Sea. The new taxa are compared with the similar Mathilda barbadensis (Dall, 1889), an amphi-atlantic mathildid recently reported from the Mediterranean Sea. The new species are clearly distinguishable from M. barbadensis, as well as from the other Mediterranean and Eastern Atlantic members of the family on the base of their teleoconch shape and sculpture, and protoconch morphology. The occurrence of M. barbadensis in the Mediterranean basin is also critically discussed

    DNA-barcoding of sympatric species of ectoparasitic gastropods of the genus Cerithiopsis (Mollusca: Gastropoda: Cerithiopsidae) from Croatia

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    The ectoparasitic gastropod genus Cerithiopsis Forbes & Hanley, 1850 was nominally based on Murex tubercularis Montagu, 1803. We have used the DNA barcode COI sequences to assay sympatric samples of morphotypes recently described as distinct species of the Cerithiopsis tubercularis-complex. Our results demonstrated that, in the Croatian waters, the gastropods usually called C. tubercularis in fact comprise a complex of cryptic species, which can be reliably diagnosed only by examining the soft parts. In the present study we have demonstrated that the colour pattern of the head-foot is diagnostic at the species level in this complex and, coupled with genetic data, may provide a sounding base for a revision of the cerithiopsids of the European coasts. Copyright © Marine Biological Association of the United Kingdom 2012

    First record of Calma gobioophaga Calado and Urgorri, 2002 (Gastropoda: Nudibranchia)in the Mediterranean sea

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    Specimens of the nudibranch genus Calma were observed under boulders at two Croatian localities while feeding on gobiid eggs. Some ambiguous morphological features compared with the original descriptions of the known species of the genus, C. glaucoides and C. gobioophaga hampered easy identification. Genetic data (COI and 16S sequences) confirmed the distinction between the two species of the genus Calma, and allowed unambiguous identification of the Croatian specimens as Calma gobioophaga. This is the first record of this species for the Mediterranean and extends its distribution range remarkably. Finally, the eggs consumed by the Croatian C. gobioophaga specimens have been taxonomically identified by using the 12S rDNA marker as Gobius cobitis

    First record of Calma gobioophaga Calado and Urgorri, 2002 (Gastropoda: Nudibranchia)in the Mediterranean sea

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    Specimens of the nudibranch genus Calma were observed under boulders at two Croatian localities while feeding on gobiid eggs. Some ambiguous morphological features compared with the original descriptions of the known species of the genus, C. glaucoides and C. gobioophaga hampered easy identification. Genetic data (COI and 16S sequences) confirmed the distinction between the two species of the genus Calma, and allowed unambiguous identification of the Croatian specimens as Calma gobioophaga. This is the first record of this species for the Mediterranean and extends its distribution range remarkably. Finally, the eggs consumed by the Croatian C. gobioophaga specimens have been taxonomically identified by using the 12S rDNA marker as Gobius cobitis

    Three new species of Raphitoma Bellardi, 1847 (Mollusca, Gastropoda, Raphitomidae) from Croatian waters (NE Adriatic Sea)

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    Three new species of the neogastropod family Raphitomidae Bellardi, 1875, are described from the Croatian waters of the Adriatic Sea: Raphitoma petanii Prkić, Giannuzzi-Savelli & Pusateri n. sp., R. pusaterii Prkić & Giannuzzi-Savelli n. sp. and R. stanici Prkić, Giannuzzi-Savelli & Pusateri n. sp. The new species are diagnosed by teleoconch characters and by genetic data (COI sequences) in an integrative taxonomy framework. They belong to a group of species with microgranules on the external surface of all teleoconch whorls and all three have a multispiral protoconch indicating a planktotrophic larval phase. The first two species are so far known only from material found in Croatia. R. petanii Prkić, Giannuzzi-Savelli & Pusateri n. sp. and R. stanici Prkić, Giannuzzi-Savelli & Pusateri n. sp. are predominantly shallow-water species, whereas R. pusaterii Prkić & Giannuzzi-Savelli n. sp. has been so far collected only from deeper bottoms with presence of the red coral, Corallium rubrum (Linnaeus, 1758). Syntypes of Murex echinatusBrocchi, 1814 are figured and commented

    Candidate cases of poecilogony in Neogastropoda: Implications for the systematics of the genus Raphitoma Bellardi, 1847

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    Poecilogony is the intraspecific variation in developmental mode, with larvae of different types produced by the same individual, population or species. It is very rare among marine invertebrates, and in gastropods has long been described only in a few opisthobranchs. The physiological and regulatory mechanisms underlying larval evolutionary transitions, such as loss of planktotrophy that occurred repeatedly in many caenogastropod lineages, are still largely unknown. We have studied the inter- v. intraspecific variation in larval development in the north-east Atlantic neogastropod genus Raphitoma Bellardi, 1847, starting with an iterative taxonomy approach: 17 morphology-based Preliminary Species Hypotheses were tested against a COI molecular-distance-based method (ABGD), and the retained species hypotheses were eventually inspected for reciprocal monophyly on a multilocus dataset. We subsequently performed an ancestral state reconstruction on an ultrametric tree of the 10 Final Species Hypotheses, time-calibrated by fossils, revealing that the interspecific changes were planktotrophy > lecithotrophy, and all have occurred in the Pleistocene, after 2.5 million years ago. This is suggestive of a major role played by Pleistocene Mediterranean oceanographic conditions-enhanced oligotrophy, unpredictable availability of water column resources-likely to favour loss of planktotrophy. Within this group of species, which has diversified after the Miocene, we identified one pair of sibling species differing in their larval development, Raphitoma cordieri (Payraudeau, 1826) and R. horrida (Monterosato, 1884). However, we also identified two Final Species Hypotheses, each comprising individuals with both larval developmental types. Our working hypothesis is that they correspond to one or two poecilogonous species. If confirmed by other nuclear markers, this would be the first documentation of poecilogony in the Neogastropoda, and the second in the whole Caenogastropoda. Although sibling species with different developmental strategies may offer good models to study some evolutionary aspects, poecilogonous taxa are optimally suited for identifying regulatory and developmental mechanisms underlying evolutionary transitions

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    Simultaneous Determination Of Potassium, Sodium, Calcium, And Magnesium In Virgin Olive Oils By Capillary Electrophoresis With Capacitively Coupled Contactless Conductivity Detection

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    Analysis of trace elements in virgin olive oil (VOO) is important for nutritional information, geographical characterization, and adulteration detection. In this study, Na+, K+, Ca2+, and Mg2+ in VOO were extracted to an aqueous solution with the aid of ultrasound energy, which was analyzed by capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D). The metal ions were separated in less than 3 min with good peak resolutions. The CE-C4D method exhibited good linearity, with coefficients of determination (R2) ranging from 0.9978 to 0.9995. The limits of quantification for Na+, K+, Ca2+, and Mg 2+ were 0.029, 0.029, 0.033, and 0.044 mg kg-1, respectively. The results of the recovery tests at three concentration levels ranged from 80.5% to 119.6% with a relative standard deviation of 0.6 to 18.1%. The proposed CE-C4D method was successfully applied for determination of the target analytes in five commercial samples of VOO. © the Partner Organisations 2014.61136293633http://www.internationaloliveoil.org, International Olive Oil Council, (accessed January 2014)Garcia-Gonzalez, D.L., Aparicio-Ruiz, R., Aparicio, R., (2008) Eur. J. Lipid Sci. Technol., 110, pp. 602-607Covas, M.I., Ruiz-Gutierrez, V., De La Torre, R., Kafatos, A., Lamuela-Raventos, R.M., Osada, J., Owen, R.W., Visioli, F., (2006) Nutr. Rev., 64, pp. 20-S30Azadmard-Damirchi, S., (2010) Food Addit. Contam., Part A, 27, pp. 1-10Dais, P., Hatzakis, E., (2013) Anal. Chim. Acta, 765, pp. 1-27Escuderos, M.E., Uceda, M., Sanchez, S., Jimenez, A., (2007) Eur. J. Lipid Sci. Technol., 109, pp. 536-546Navas, M.J., Jimenez, A.M., (2007) J. Am. Oil Chem. Soc., 84, pp. 405-411Monasterio, R.P., Fernandez, M.D., Silva, M.F., (2013) J. Agric. Food Chem., 61, pp. 4477-4496Brkljaca, M., Giljanovic, J., Prkic, A., (2013) Anal. Lett., 46, pp. 2912-2926Arvanitoyannis, I.S., Vlachos, A., (2007) Crit. Rev. Food Sci. Nutr., 47, pp. 441-498Yasar, S.B., Baran, E.K., Alkan, M., (2012) Olive Oil - Constituents, Quality, Health Properties and Bioconversions, 1. , in, ed. D. Boskou, InTech, 1st edn, ch. 5, 89-108Mendil, D., Uluozlu, O.D., Tuzen, M., Soylak, M., (2009) J. Hazard. Mater., 165, pp. 724-728Baran, E.K., Yasar, S.B., (2012) Eur. J. Lipid Sci. Technol., 114, pp. 1320-1326Ieggli, C.V.S., Bohrer, D., Do Nascimento, P.C., De Carvalho, L.M., (2011) Food Addit. Contam., Part A, 28, pp. 640-648Cindric, I.J., Zeiner, M., Steffan, I., (2007) Microchem. J., 85, pp. 136-139Robaina, N.F., Brum, D.M., Cassella, R.J., (2012) Talanta, 99, pp. 104-112Zeiner, M., Steffan, I., Cindric, I.J., (2005) Microchem. J., 81, pp. 171-176Bakircioglu, D., Kurtulus, Y.B., Yurtsever, S., (2013) Food Chem., 138, pp. 770-775Zhu, F.K., Fan, W.X., Wang, X.J., Qu, L., Yao, S.W., (2011) Food Chem. Toxicol., 49, pp. 3081-3085Llorent-Martinez, E.J., Ortega-Barrales, P., Fernandez-De Cordova, M.L., Dominguez-Vidal, A., Ruiz-Medina, A., (2011) Food Chem., 127, pp. 1257-1262Castillo, J.R., Jimenez, M.S., Ebdon, L., (1999) J. Anal. At. Spectrom., 14, pp. 1515-1518Dugo, G., Pellicano, T.M., La Pera, L., Lo Turco, V., Tamborrino, A., Clodoveo, M.L., (2007) Food Chem., 102, pp. 599-605Buldini, P.L., Ferri, D., Sharma, J.L., (1997) J. Chromatogr. A, 789, pp. 549-555Nogueira, T., Do Lago, C.L., (2011) Microchem. J., 99, pp. 267-272De Caland, L.B., Silveira, E.L.C., Tubino, M., (2012) Anal. Chim. Acta, 718, pp. 116-120Da Silva, J.A.F., Ricelli, N.L., Carvalho, A.Z., Do Lago, C.L., (2003) J. Braz. Chem. Soc., 14, pp. 265-268Ribani, M., Bottoli, C.B.G., Collins, C.H., Jardim, I.C.S.F., Melo, L.F.C., (2004) Quim. Nova, 27, pp. 771-780Benincasa, C., Lewis, J., Perri, E., Sindona, G., Tagarelli, A., (2007) Anal. Chim. Acta, 585, pp. 366-370Danzer, K., Currie, L.A., Chem, C.G.A.A., (1998) Pure Appl. Chem., 70, pp. 993-1014Velasco, J., Dobarganes, C., (2002) Eur. J. Lipid Sci. Technol., 104, pp. 661-67
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