309 research outputs found
Depolarization and decreased surface expression of K+ channels contribute to NSAID-inhibition of intestinal restitution
No full textNon-steroidal anti-inflammatory drugs (NSAIDs) contribute to gastrointestinal ulcer formation by inhibiting epithelial cell migration and mucosal restitution; however, the drug-affected signaling pathways are poorly defined. We investigated whether NSAID inhibition of intestinal epithelial migration is associated with depletion of intracellular polyamines, depolarization of membrane potential (Em) and altered surface expression of K+ channels. Epithelial cell migration in response to the wounding of confluent IEC-6 and IEC-Cdx2 monolayers was reduced by indomethacin (100μM), phenylbutazone (100μM) and NS-398 (100μM) but not by SC-560 (1μM). NSAID-inhibition of intestinal cell migration was not associated with depletion of intracellular polyamines. Treatment of IEC-6 and IEC-Cdx2 cells with indomethacin, phenylbutazone and NS-398 induced significant depolarization of Em, whereas treatment with SC-560 had no effect on Em. The Em of IEC-Cdx2 cells was: −38.5±1.8mV under control conditions; −35.9±1.6mV after treatment with SC-560; −18.8±1.2mV after treatment with indomethacin; and −23.7±1.4mV after treatment with NS-398. Whereas SC-560 had no significant effects on the total cellular expression of Kv1.4 channel protein, indomethacin and NS-398 decreased not only the total cellular expression of Kv1.4, but also the cell surface expression of both Kv1.4 and Kv1.6 channel subunits in IEC-Cdx2. Both Kv1.4 and Kv1.6 channel proteins were immunoprecipitated by Kv1.4 antibody from IEC-Cdx2 lysates, indicating that these subunits co-assemble to form heteromeric Kv channels. These results suggest that NSAID inhibition of epithelial cell migration is independent of polyamine-depletion, and is associated with depolarization of Em and decreased surface expression of heteromeric Kv1 channels.ID: S0006295207001931; M3: Article; Accession Number: S0006295207001931; Author: L.C. Freeman (b); Author: D.F. Narvaez (a); Author: A. McCoy (a); Author: F.B. von Stein (c); Author: S. Young (b); Author: K. Silver (a); Author: S. Ganta (b); Author: D. Koch (b); Author: R. Hunter (b); Author: R.F. Gilmour (c); Author: J.D. Lillich (a, ⁎); Affiliation: Department of Clinical Sciences, Kansas State University, Manhattan, KS 66506, United States; Affiliation: Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, United States; Affiliation: Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853, United States; Keyword: Non-steroidal anti-inflammatory drugs; Keyword: Intestinal epithelial cells; Keyword: Membrane potential; Keyword: Potassium channels; Number of Pages: 12; Language: English;Source type: Electronic(1)http://search.ebscohost.com/login.aspx?direct=true&db=edselp&AN=S0006295207001931&site=eds-live&scope=sit
Porcine vas deferens luminal pH is acutely increased by systemic xylazine administration
No full textData are accumulating to demonstrate that pH regulation in the male reproductive tract has a vital role in modulating sperm cell fertilizing capacity and, therefore, male fertility. Bicarbonate uptake by sperm cells is required for the achievement of motility levels required for fertilization. Vas deferens epithelial cells can carry out measurable bicarbonate secretion, but the available literature to date reports that the vas deferens luminal content is typically acidic. This study aimed to determine pH in the boar vas deferens lumen and whether modulatory mechanisms exist for regulation of pH in this compartment of the male reproductive tract. A fiber-optic pH probe was used to assess pH in the vas deferens of anesthetized adult boars. The mean pH, derived from multiple measurements at variable positions along the vas deferens lumen, was 7.39 ± 0.09. Furthermore, administration of xylazine, an alpha-2 adrenergic receptor agonist, rapidly (< 10 min) alkalinized the vas deferens lumen in most cases. Since the duct was transected proximal to the site of measurements, the observations rule out the possibility that alkalinization resulted from secretion in more proximal portions of the duct. These results indicate that the boar vas deferens lumen can be alkaline, and suggest that porcine vas deferens epithelia increase net bicarbonate secretion in vivo, following systemic alpha-2 adrenergic stimulation. This secretory response greatly changes the luminal environment to which sperm cells are exposed, which will initiate or enhance motility, and is expected to modulate male fertility
Stained Glass before 1700 in American Collections, New En- gland and New York (Corpus Vitrearum Checklist, I), sous la direction de J. Hayward et M. Caviness, avec la collaboration de M. Parsons Lillich, L. Morey Papanicolaou, V. Chieffo Raguin, H. Jackson Zakin
No full textBrisac Catherine. Stained Glass before 1700 in American Collections, New En- gland and New York (Corpus Vitrearum Checklist, I), sous la direction de J. Hayward et M. Caviness, avec la collaboration de M. Parsons Lillich, L. Morey Papanicolaou, V. Chieffo Raguin, H. Jackson Zakin. In: Bulletin Monumental, tome 144, n°4, année 1986. pp. 372-373
Stained Glass before 1700 in American Collections, New En- gland and New York (Corpus Vitrearum Checklist, I), sous la direction de J. Hayward et M. Caviness, avec la collaboration de M. Parsons Lillich, L. Morey Papanicolaou, V. Chieffo Raguin, H. Jackson Zakin
No full textBrisac Catherine. Stained Glass before 1700 in American Collections, New En- gland and New York (Corpus Vitrearum Checklist, I), sous la direction de J. Hayward et M. Caviness, avec la collaboration de M. Parsons Lillich, L. Morey Papanicolaou, V. Chieffo Raguin, H. Jackson Zakin. In: Bulletin Monumental, tome 144, n°4, année 1986. pp. 372-373
Structural and functional basis for the long QT syndrome: relevance to veterinary patients
No full textLong QT syndrome (LQTS) is a condition characterized by prolongation of ventricular repolarization and is manifested clinically by lengthening of the QT interval on the surface ECG. Whereas inherited forms of LQTS associated with mutations in the genes that encode ion channel proteins are identified only in humans, the acquired form of LQTS occurs in humans and companion animal species. Often, acquired LQTS is associated with drug-induced block of the cardiac K+ current designated I(Kr). However, not all drugs that induce potentially fatal ventricular arrhythmias antagonize I(Kr), and not all drugs that block I(Kr), are associated with ventricular arrhythmias. In clinical practice, the extent of QT interval prolongation and risk of ventricular arrhythmia associated with antagonism of I(Kr) are modulated by pharmacokinetic and pharmacodynamic variables. Veterinarians can influence some of the potential risk factors (eg, drug dosage, route of drug administration, presence or absence of concurrent drug therapy, and patient electrolyte status) but not all (eg, patient gender/genetic background). Veterinarians need to be aware of the potential for acquired LQTS during therapy with drugs identified as blockers of HERG channels and I(Kr).Accession Number: 12892298. Language: English. Language Code: eng. Date Revised: 20071114. Date Created: 20030801. Date Completed: 20030912. Update Code: 20111122. Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review. Journal ID: 8708660. Publication Model: Print. Cited Medium: Print. NLM ISO Abbr: J. Vet. Intern. Med. Linking ISSN: 08916640. Subset: IM. Date of Electronic Publication: 20030701; ID: 1289229
MEASUREMENT OF ISOLATED PROMPT PHOTON PRODUCTION IN PHOTON-PHOTON COLLISIONS AT WITH THE OPAL DETECTOR AT LEP
No full textExpression and coassociation of ERG1, KCNQ1, and KCNE1 potassium channel proteins in horse heart
No full textIn dogs and in humans, potassium channels formed by ether-a-go-go-related gene 1 protein ERG1 (KCNH2) and KCNQ1 alpha-subunits, in association with KCNE beta-subunits, play a role in normal repolarization and may contribute to abnormal repolarization associated with long QT syndrome (LQTS). The molecular basis of repolarization in horse heart is unknown, although horses exhibit common cardiac arrhythmias and may receive drugs that induce LQTS. In horse heart, we have used immunoblotting and immunostaining to demonstrate the expression of ERG1, KCNQ1, KCNE1, and KCNE3 proteins and RT-PCR to detect KCNE2 message. Peptide N-glycosidase F-sensitive forms of horse ERG1 (145 kDa) and KCNQ1 (75 kDa) were detected. Both ERG1 and KCNQ1 coimmunoprecipitated with KCNE1. Cardiac action potential duration was prolonged by antagonists of either ERG1 (MK-499, cisapride) or KCNQ1/KCNE1 (chromanol 293B). Patch-clamp analysis confirmed the presence of a slow delayed rectifier current. These data suggest that repolarizing currents in horses are similar to those of other species, and that horses are therefore at risk for acquired LQTS. The data also provide unique evidence for coassociation between ERG1 and KCNE1 in cardiac tissue.Accession Number: 12063283. Language: English. Language Code: eng. Date Revised: 20081028. Date Created: 20020613. Date Completed: 20020716. Update Code: 20111122. Publication Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.. Journal ID: 100901228. Publication Model: Print. Cited Medium: Print. NLM ISO Abbr: Am. J. Physiol. Heart Circ. Physiol. Linking ISSN: 03636135. Subset: IM. Date of Electronic Publication: 20020701; ID: 1206328
Books Received
No full textBooks Received
The Human Rights of Aliens in Contemporary International Law By Richard B. Lillich Dover, New Hampshire: Manchester University Press, 1985. Pp. xii, 126. 25.00
Emerging Standards of International Trade and Investment Edited by Seymour J. Rubin and Gary Clyde Hufbauer Totowa, New Jersey: Rowman & Allanheld, 1984. Pp. ix,196
The World of International Tax Planning By Milton Grundy New York: Cambridge University Press, 1984. Pp. x, 86.39.50
The United Nations and the Control of International Violence By John F. Murphy Totowa, New Jersey: Allenheld, Osmun & Co., 1982. Pp. xii, 206. 34.50
Consensus and Confrontation: The United States and the Law of the Sea Convention Edited by Jon M. Van Dyke Honolulu: The Law of the Sea Institute, 1985. Pp. x, 549. 75.0
Investigation of the decay of orbitally excited B mesons and first measurement of the branching ratio BR(B*(J) ---> B*pi(X))
No full textFrom about 4 million hadronic Z decays recorded by the OPAL detector on and near to the Z resonance, we select a sample of more than 570000 inclusively reconstructed B mesons. Orbitally-excited mesons B*J are reconstructed using Bpi+- combinations. Independently, B* mesons are reconstructed using the decay B* -> Bgamma. The selected B* candidates are used to obtain samples enriched or depleted in the decay B*J -> B*pi+-(X), where (X) refers to decay modes with or without additional accompanying decay particles. From the number of signal candidates in the Bpi+- mass spectra of these two samples, we perform the first measurement of the branching ratio of orbitally-excited B mesons decaying into B*pi(X): BR(B*J ->B*pi(X)) = 0.85 +0.36-0.37 +- 0.12, where the first error is statistical and the second systematic. If B*J decay modes other than single pion transitions can be neglected the measured ratio corresponds to the branching ratio BR(B*J->B*pi). In the framework of Heavy Quark Symmetry, a simultaneous fit to the Bpi+- mass spectra of the samples enriched or depleted in B*J->B*pi+-(X) decays yields the mass and the width of the B1(3/2) state, as well as the branching ratio of B*J mesons decaying into B*pi: M(B1(3/2)) = (5.738 +0.005-0.006 +-0.007) GeV/c2 G(B1(3/2)) = (18 +15-13 +29-23) MeV/c BR(B*J->B*pi) = 0.74 +0.12-0.10 +0.21-0.15, where the uncertainties are statistical and systematic, respectively.From about 4 million hadronic Z decays recorded by the OPAL detector on and near to the Z resonance, we select a sample of more than 570000 inclusively reconstructed B mesons. Orbitally-excited mesons B*J are reconstructed using Bpi+- combinations. Independently, B* mesons are reconstructed using the decay B* -> Bgamma. The selected B* candidates are used to obtain samples enriched or depleted in the decay B*J -> B*pi+-(X), where (X) refers to decay modes with or without additional accompanying decay particles. From the number of signal candidates in the Bpi+- mass spectra of these two samples, we perform the first measurement of the branching ratio of orbitally-excited B mesons decaying into B*pi(X): BR(B*J ->B*pi(X)) = 0.85 +0.36-0.37 +- 0.12, where the first error is statistical and the second systematic. If B*J decay modes other than single pion transitions can be neglected the measured ratio corresponds to the branching ratio BR(B*J->B*pi). In the framework of Heavy Quark Symmetry, a simultaneous fit to the Bpi+- mass spectra of the samples enriched or depleted in B*J->B*pi+-(X) decays yields the mass and the width of the B1(3/2) state, as well as the branching ratio of B*J mesons decaying into B*pi: M(B1(3/2)) = (5.738 +0.005-0.006 +-0.007) GeV/c2 G(B1(3/2)) = (18 +15-13 +29-23) MeV/c BR(B*J->B*pi) = 0.74 +0.12-0.10 +0.21-0.15, where the uncertainties are statistical and systematic, respectively
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